##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR841055.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2376381 Sequences flagged as poor quality 0 Sequence length 24-50 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.95668160955672 39.0 37.0 39.0 32.0 39.0 2 37.65268111468657 39.0 37.0 39.0 35.0 39.0 3 37.88783743010906 39.0 38.0 39.0 35.0 39.0 4 38.31512034475953 39.0 38.0 40.0 35.0 41.0 5 39.04135616300585 40.0 38.0 41.0 35.0 41.0 6 38.96689966802462 40.0 38.0 41.0 35.0 41.0 7 39.01665852403297 40.0 38.0 41.0 35.0 41.0 8 38.99491369439497 40.0 38.0 41.0 35.0 41.0 9 39.009712247320614 40.0 38.0 41.0 35.0 41.0 10 38.99676019964812 40.0 38.0 41.0 35.0 41.0 11 38.87368103010418 40.0 38.0 41.0 35.0 41.0 12 38.81188875016254 40.0 38.0 41.0 35.0 41.0 13 38.698388011013385 40.0 38.0 41.0 35.0 41.0 14 38.61310539008686 40.0 38.0 41.0 35.0 41.0 15 38.541309663728164 40.0 38.0 41.0 35.0 41.0 16 38.522938030559914 40.0 38.0 41.0 35.0 41.0 17 38.52824568114288 40.0 38.0 41.0 35.0 41.0 18 38.51077415616435 40.0 38.0 41.0 35.0 41.0 19 38.48552652121019 40.0 38.0 41.0 35.0 41.0 20 38.41343454605975 40.0 37.0 41.0 35.0 41.0 21 38.335216028069574 40.0 37.0 41.0 34.0 41.0 22 38.25446130060794 40.0 37.0 41.0 34.0 41.0 23 38.17450821227741 40.0 37.0 41.0 34.0 41.0 24 37.96315195248573 39.0 36.0 41.0 33.0 41.0 25 37.856127032986194 39.0 36.0 41.0 33.0 41.0 26 37.71661725239617 39.0 36.0 41.0 33.0 41.0 27 37.59540662857221 39.0 36.0 41.0 33.0 41.0 28 37.42536832649259 39.0 35.0 40.0 33.0 41.0 29 37.42172283763747 39.0 35.0 40.0 33.0 41.0 30 37.36068643908083 38.0 35.0 40.0 33.0 41.0 31 37.31780736774586 38.0 35.0 40.0 33.0 41.0 32 37.21748702115332 38.0 35.0 40.0 33.0 41.0 33 37.06513219895112 38.0 35.0 40.0 32.0 41.0 34 36.97123614158464 38.0 35.0 40.0 32.0 41.0 35 36.808296984398055 38.0 35.0 40.0 32.0 41.0 36 36.769863177287924 38.0 35.0 40.0 32.0 41.0 37 36.63155143190473 37.0 35.0 40.0 32.0 41.0 38 36.44436426919077 37.0 34.0 40.0 31.0 41.0 39 36.397675339279886 37.0 34.0 40.0 31.0 41.0 40 36.11145294796087 36.0 34.0 40.0 31.0 41.0 41 35.939323237617636 36.0 34.0 39.0 31.0 41.0 42 35.81758412763868 36.0 34.0 39.0 30.0 41.0 43 35.67470347456342 36.0 34.0 39.0 30.0 41.0 44 35.30275407140236 36.0 33.0 39.0 29.0 41.0 45 35.07729517189514 36.0 33.0 39.0 29.0 41.0 46 34.69023935711264 35.0 33.0 39.0 28.0 40.0 47 34.74150350784938 35.0 33.0 38.0 29.0 40.0 48 35.520318008074035 36.0 34.0 39.0 31.0 40.0 49 36.17299523295448 37.0 35.0 39.0 32.0 41.0 50 36.001487666658726 37.0 34.0 39.0 31.0 40.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 20 11.0 21 27.0 22 78.0 23 158.0 24 350.0 25 582.0 26 941.0 27 1976.0 28 4121.0 29 8520.0 30 17309.0 31 32639.0 32 58057.0 33 99833.0 34 149952.0 35 178916.0 36 288545.0 37 411619.0 38 393194.0 39 374028.0 40 355525.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 0.0 45.65383244521817 18.355894951188382 35.990272603593446 2 23.003213710259423 24.57871864823023 33.68508669274834 18.732980948762005 3 25.219062094840854 27.306564056857884 23.13875594864628 24.33561789965498 4 18.58266835158167 29.079974970343557 28.762980347006646 23.574376331068127 5 16.624059862454715 30.69120650266098 27.793775493071188 24.89095814181312 6 21.702580520547844 27.22909331458213 24.994392734161735 26.07393343070829 7 21.681035153874735 26.017797651134224 22.583247383311008 29.717919811680026 8 19.38211928137786 25.978157542919256 28.954405880201872 25.685317295501015 9 22.003247795702794 31.524448310266745 25.651358094514304 20.820945799516156 10 30.16658524032973 23.23886615824651 24.404462079102636 22.190086522321124 11 22.054039314402868 26.454512134207437 23.551526459772234 27.939922091617465 12 21.007279556603088 26.366058304623714 25.17369899860334 27.45296314016986 13 20.91411267805962 27.305512037000803 23.036036729800482 28.744338555139098 14 18.305187594076873 27.275929238619568 24.07795719625767 30.34092597104589 15 19.03592058680826 35.27464661600981 23.942541200253665 21.74689159692827 16 18.94986536249869 37.705359536202316 21.159064981583338 22.18571011971565 17 20.245995907221946 36.65851561681397 19.342352930780038 23.753135545184044 18 20.058273483923664 29.68433933784187 21.95948376964805 28.297903408586418 19 21.34190603274475 26.484557821325787 23.879377928034266 28.294158217895195 20 20.40148444209914 26.80007961686278 26.1248932725855 26.673542668452576 21 20.29539875971067 35.239130425634606 22.68516706706542 21.780303747589297 22 22.988779997820217 29.228183527809726 20.571196285444127 27.211840188925933 23 23.794795531524617 26.513383165409927 27.236667857553144 22.455153445512316 24 21.280047265148138 28.632740288699498 23.342763639332244 26.74444880682012 25 20.544192462728674 28.03943156175753 24.262711150561298 27.153664824952493 26 21.440638977635782 34.65972310969116 24.473099041533548 19.42653887113951 27 23.189370138155276 30.161615607293278 20.903745764107068 25.745268490444374 28 22.650205092729223 27.650855392018126 21.305403446975117 28.393536068277538 29 22.26221478093626 29.402976108073943 27.082478993023358 21.25233011796644 30 23.608341405048144 29.851641151282198 27.333353786547338 19.20666365712232 31 21.88145278972708 33.35842797609128 22.953154652993256 21.80696458118839 32 19.441702660919454 29.0965583471807 25.032864317761682 26.428874674138164 33 20.030490387058524 25.729826669946938 31.738727317621198 22.500955625373344 34 20.55328665757305 32.69011089436038 24.467958068422032 22.288644379644538 35 18.231393152259436 27.586575730876472 25.04400849894307 29.138022617921024 36 19.711577467690457 27.549051156506987 25.5864579335568 27.152913442245758 37 16.90894032446235 36.79468694739777 22.319993836536135 23.976378891603748 38 24.67720489380726 27.29250731496664 23.635393088913638 24.39489470231246 39 19.81159703467452 32.24148661700323 26.068242585642036 21.878673762680208 40 18.511572305041526 27.721102911807648 26.31627194332222 27.4510528398286 41 18.730527498784074 33.02673145301809 24.80679784486219 23.435943203335647 42 19.431509585710455 33.8854305105633 23.67011689704974 23.012943006676498 43 19.350471528985977 26.771151425896917 23.309389911998903 30.568987133118203 44 18.6613872693984 27.621808469929803 22.094777968998667 31.622026291673127 45 17.761440462245275 26.368189998406155 32.40034228928396 23.470027250064604 46 18.814693743748855 29.448606582969877 28.608464445025433 23.128235228255832 47 18.71959586092141 32.99691358234653 26.406388075012803 21.877102481719252 48 18.21273188088732 32.97922050045819 24.03635793245218 24.771689686202315 49 18.119746242767555 28.78252325905799 22.23481034921944 30.86292014895501 50 18.31735589447311 29.31071444812331 25.940835475327905 26.431094182075675 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 61409.0 1 54796.0 2 37925.0 3 25963.5 4 21605.0 5 15027.5 6 8317.5 7 5504.5 8 5344.0 9 4994.5 10 4541.0 11 4263.5 12 4104.0 13 3946.0 14 4094.0 15 4324.5 16 4261.5 17 4055.0 18 4059.5 19 4520.5 20 5209.0 21 6298.0 22 7741.5 23 9523.0 24 11368.5 25 13183.5 26 15262.5 27 17263.5 28 19337.5 29 23259.0 30 31052.0 31 45388.0 32 66749.0 33 78818.0 34 86866.0 35 100613.5 36 103858.0 37 102702.5 38 104860.0 39 108472.5 40 119505.0 41 138037.5 42 152755.0 43 162205.5 44 169494.0 45 171150.5 46 172596.5 47 179975.5 48 228189.5 49 286757.0 50 279647.0 51 251964.5 52 235223.0 53 201794.0 54 171816.0 55 156346.0 56 144199.0 57 133362.5 58 119708.5 59 101344.0 60 85048.5 61 69589.5 62 54266.0 63 42959.0 64 34346.0 65 28096.5 66 20162.0 67 12607.5 68 8250.0 69 5728.0 70 4139.5 71 3392.5 72 2914.0 73 2232.0 74 1549.5 75 931.0 76 591.0 77 413.5 78 264.0 79 226.5 80 189.0 81 113.5 82 47.0 83 82.5 84 126.5 85 116.5 86 110.5 87 56.0 88 2.5 89 1.5 90 1.0 91 1.0 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 >>END_MODULE >>Sequence Length Distribution warn #Length Count 24 13908.0 25 14973.0 26 15052.0 27 18129.0 28 17225.0 29 15464.0 30 19527.0 31 14985.0 32 15595.0 33 12384.0 34 12747.0 35 12458.0 36 13342.0 37 13118.0 38 12837.0 39 16515.0 40 20392.0 41 17677.0 42 22238.0 43 39451.0 44 99653.0 45 206028.0 46 262694.0 47 258699.0 48 386880.0 49 362613.0 50 461797.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 11.24758088533613 #Duplication Level Percentage of deduplicated Percentage of total 1 70.63065630852991 7.944240198145668 2 11.274564696876158 2.536231567501396 3 4.115081774705283 1.3885414533231066 4 2.0455844198153157 0.9203150487862457 5 1.1980151058104984 0.6737385902229052 6 0.8509012534656952 0.5742348404273585 7 0.620803873029642 0.4887779243081588 8 0.45243296537851374 0.4071021098629852 9 0.3933099843831771 0.39814072761240665 >10 4.349070047922041 11.864369357941685 >50 2.1814935728380265 17.794294671033327 >100 1.7795086198800159 33.15246282272906 >500 0.06340587304183569 4.835336232542731 >1k 0.039784077202720425 9.105083182235983 >5k 0.003315339766893369 2.57964460859887 >10k+ 0.0020720873543083555 5.337486664728114 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAAC 43101 1.8137243144091795 No Hit CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTA 28472 1.1981243748372 No Hit CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCT 16410 0.6905458341907295 No Hit ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATCAT 16306 0.686169431585255 No Hit CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAACC 10220 0.4300657175764324 No Hit ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATC 9570 0.40271320129221705 No Hit CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAA 7468 0.3142593717084929 No Hit TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCTA 7407 0.31169244325720497 No Hit CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACACA 6943 0.2921669547097035 No Hit ATAGGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACATGC 6889 0.2898945918183995 No Hit ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTA 6078 0.2557670676545554 No Hit CAAAGCACCCAACTTACACTTAGGAGATTTCAACTTAACTTGACCGCTCT 5506 0.23169685332444587 No Hit CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTT 5482 0.2306869142616441 No Hit AACACAAAGCACCCAACTTACACTTAGGAGATTTCAACTTAACTTGACCG 4749 0.19984169205190583 No Hit TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTC 4661 0.1961385821549659 No Hit CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACC 4608 0.19390830005794527 No Hit CGGCAAGTTTGAATTTCGTGGAGGCTCGGG 4513 0.18991062460102145 No Hit ATAGGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACAT 4470 0.18810115044683492 No Hit CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACA 4083 0.1718158830591559 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAA 3986 0.16773404601366532 No Hit CCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCTAG 3750 0.15780297856278097 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAA 3729 0.1569192818828294 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAA 3659 0.1539736262829908 No Hit TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 3638 0.15308992960303924 No Hit CAAAGCACCCAACTTACACTTAGGAGATTTCAACTTAACTTGACCGCT 3567 0.15010219320891724 No Hit AACACAAAGCACCCAACTTACACTTAGGAGATTTCAACTTAACTTGAC 3283 0.1381512476324293 No Hit TAGCTGTTAGTTGGTAGAGGGAAATTCAGGCTACCGTCGCGAAACCTGC 3276 0.13785668207244545 No Hit AGGTTTTCTGTTTCCTGTGATACCTTGGGCTGGAATCTGAAGCAATGAAG 3268 0.13752003571817817 No Hit AGCTTTAATTTATTAATGCAAACAGTACCTAACAAACCCACAGGTCCT 3120 0.13129207816423377 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAA 3086 0.12986133115859788 No Hit ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAGAA 3076 0.1294405232157638 No Hit AAAAAAAAAAAAAAAAAAAAAAAAA 3050 0.12834642256439519 No Hit ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAGAAT 2901 0.12207638421616737 No Hit CAGAGTGTTGCTTAACACAAAGCACCCAACTTACACTTAGGAGATTTCAA 2828 0.11900448623347855 No Hit ACAACCTTAACCAAACCATTTACCCAAATAAAGTATAGGCGATAGAAAT 2682 0.11286069026810094 No Hit TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACT 2658 0.11185075120529915 No Hit AATGCCCCAACTAAATACTACCGTATGGCCCACCATAATTACCCCCATAC 2619 0.11020960022824623 No Hit CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAA 2551 0.10734810621697446 No Hit ATAGGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTACAC 2515 0.10583319762277177 No Hit AAAAAAAAAAAAAAAAAAAAAAAA 2463 0.10364499632003454 No Hit CTGATGTTCGCCGACCGTTGACTATTCTCTACAAACCACAAAGACATTGG 2463 0.10364499632003454 No Hit CCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCT 2444 0.10284546122864978 No Hit AGGTTTTCTGTTTCCTGTGATACCTTGGGCTGGAATCTGAAGCAATGAA 2389 0.10053101754306233 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.027983728198466493 0.0 0.0 0.0 0.0 2 0.0627003834822783 0.0 0.0 0.0 0.0 3 0.09741703876609012 0.0 0.0 0.0 0.0 4 0.13024005830714855 0.0 0.0 0.0 0.0 5 0.15759257459136392 0.0 0.0 0.0 0.0 6 0.19533904706358113 0.0 0.0 0.0 0.0 7 0.2267313195990037 0.0 0.0 0.0 0.0 8 0.2617425404427994 0.0 0.0 0.0 0.0 9 0.2918303083554363 0.0 0.0 0.0 0.0 10 0.32482165107362837 0.0 0.0 0.0 0.0 11 0.35339451039206254 0.0 0.0 0.0 0.0 12 0.38693290343593895 0.0 0.0 0.0 0.0 13 0.42072378124551574 0.0 0.0 0.0 0.0 14 0.45165316504382086 0.0 0.0 0.0 0.0 15 0.4830875183735268 0.0 0.0 0.0 0.0 16 0.5137644174061314 0.0 0.0 0.0 0.0 17 0.5481865071299594 0.0 0.0 0.0 0.0 18 0.5866483531049945 0.0 0.0 0.0 0.0 19 0.6162311514862305 0.0 0.0 0.0 0.0 20 0.6427420518847777 0.0 0.0 0.0 0.0 21 0.6693791946661751 0.0 0.0 0.0 0.0 22 0.6918924196077986 0.0 0.0 0.0 0.0 23 0.7215172987833179 0.0 0.0 0.0 0.0 24 0.748112360770432 0.0 0.0 0.0 0.0 25 0.7767273008831497 0.0 0.0 0.0 0.0 26 0.8091715932756574 0.0 0.0 0.0 0.0 27 0.835724574468488 0.0 0.0 0.0 0.0 28 0.8651811304668738 0.0 0.0 0.0 0.0 29 0.8968679685622802 0.0 0.0 0.0 0.0 30 0.928807291423387 0.0 0.0 0.0 0.0 31 0.9594421096617083 0.0 0.0 0.0 0.0 32 0.9878466458030088 0.0 0.0 0.0 0.0 33 1.0178923329213623 0.0 0.0 0.0 0.0 34 1.047433050508315 0.0 0.0 0.0 0.0 35 1.073102335021194 0.0 0.0 0.0 0.0 36 1.0951526712256998 0.0 0.0 0.0 0.0 37 1.116403472338821 0.0 0.0 0.0 0.0 38 1.1299114073037952 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTTTCGC 20 3.994337E-7 202.90834 44 CATGCGA 25 4.980393E-9 202.90831 44 CGATCAG 45 5.456968E-12 157.81758 44 CCGCCGT 210 0.0 135.27222 44 CGTCGCC 15 0.008725307 135.2722 44 CTATCAT 3245 0.0 122.87052 44 ATGCGAA 70 1.8189894E-12 115.94762 44 TGCCCGG 80 5.456968E-12 101.45417 44 TATACAC 40 1.2573866E-5 101.45417 44 ACTTCTA 1630 0.0 98.96449 44 CTTAACC 2360 0.0 94.57591 44 CCTTAAC 6900 0.0 94.20602 43 CCGATAA 100 0.0 91.30874 44 ACATTGG 765 0.0 90.18147 44 ACCAGCG 45 2.25658E-5 90.18147 44 ATCGGCT 45 2.25658E-5 90.18147 44 CCAGAAT 715 0.0 89.39317 44 AATGCGA 55 2.910383E-11 83.15618 43 TAATTCC 550 0.0 79.3187 44 ATGCGTT 25 1.4603793E-6 78.29546 42 >>END_MODULE