FastQCFastQC Report
Tue 24 May 2016
ERR841054.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR841054.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1825226
Sequences flagged as poor quality0
Sequence length24-50
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGC312561.7124454725058704No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTA210021.1506520288446473No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCT123250.6752588446581409No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATCAT116010.6355925238847135No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACACA83180.45572438700741713No Hit
CGGCAAGTTTGAATTTCGTGGAGGCTCGGG78000.427344339824219No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATC68260.3739810850820665No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAGAA67180.36806400960757735No Hit
AGCTTTAATTTATTAATGCAAACAGTACCTAACAAACCCACAGGTCCT54580.2990314624052035No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACA52430.28725209919210004No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCTA46860.2567353303097808No Hit
TAGCTGTTAGTTGGTAGAGGGAAATTCAGGCTACCGTCGCGAAACCTG44560.24413415105855382No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTA44240.24238094351055703No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAG38740.2122476887793621No Hit
AGGTTTTCTGTTTCCTGTGATACCTTGGGCTGGAATCTGAAGCAATGAA33290.1823883727275417No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACC32550.1783340802727991No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAA32020.1754303302714294No Hit
ACAACCTTAGCCAAACCATTTACCCAAATAAAGTATAGGCGATAGAAAT31550.1728553066853091No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAA31190.1708829481938127No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTC30130.16507544819107334No Hit
AGCTTTAATTTATTAATGCAAACAGTACCTAACAAACCCACAGGTC30070.16474672177582392No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAA29720.16282915102020243No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAA28980.15877485856545984No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCC27960.15318650950622006No Hit
AACACAAAGCACCCAACTTACACTTAGGAGATTTCAACTTAACTTGACCG27220.14913221705147747No Hit
CAAACAGCCGGGGCTCCAGCGGGAGAACGATAATGCAAAGTGCTATGT26430.14480398591736038No Hit
CTGATGTTCGCCGACCGTTGACTATTCTCTACAAACCACAAAGACATTGG25250.13833903308412218No Hit
CACTTAGGAGATTTCAACTTAACTTGACCGCTCTGACCAAAAAAAAAAAA23600.12929905666476368No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA23480.1286416038342649No Hit
AGCTTTAATTTATTAATGCAAACAGTACCTAACAAACCCACAGG23110.1266144576068936No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAA23090.12650488213514383No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAGAAT22970.12584742930464501No Hit
CCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCTAG22610.12387507081314862No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA22390.12266974062390082No Hit
TAGCTGTTAGTTGGTAGAGGGAAATTCAGGCTACCGTCGCGAAACC21740.11910853779203232No Hit
TTGAGTTCCAGCCAGGAGGACCAGGGGCTGGG21490.11773884439515983No Hit
ACAACCTTAGCCAAACCATTTACCCAAATAAAGTATAGGCGATAGAA20990.11499945760141483No Hit
CGGCAAGTTTGAATTTCGTGGAGGCTCGGGTTGTGAGGG20600.11286273590229373No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTT19810.10853450476817665No Hit
ATAGGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACATGC19540.10705523589955436No Hit
AGGTTTTCTGTTTCCTGTGATACCTTGGGCTGGAATCTGAAGCAATG18980.10398712269055997No Hit
AGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTACTTTGAAAAAATTAGAGT18900.10354882080356076No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCGAT204.8163027E-5183.4280544
GTCTTAT150.004991222163.0471544
CTTAACG251.17202006E-4146.7424544
GGTCGAG505.966285E-10146.7424544
AGCGATA551.1586963E-9133.4022244
CTATCAT22050.0133.0997244
CACACGT152.1072538E-4126.6576643
AGGTACG302.4223758E-4122.28535544
GAACACA17900.0107.93902644
TCGCAAA1300.0102.3004243
AATCGCA1950.0100.3367144
CTTCGTT850.096.8558543
ACTTCTA11400.096.5410844
AATGTGC903.255991E-1095.1108444
ACGTTAA206.618082E-494.9932543
GGTCCGT206.618082E-494.9932543
AGGTCGA401.2314558E-894.9932543
ACATTGG7550.089.0820644
GACACGA552.4165241E-588.93481444
TTACGAG650.087.6860843
Produced by FastQC (version 0.11.5)