##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR841054.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1825226
Sequences flagged as poor quality	0
Sequence length	24-50
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	35.950214932287835	39.0	35.0	39.0	31.0	39.0
2	36.744133055303834	39.0	35.0	39.0	33.0	39.0
3	37.04900653398538	39.0	35.0	39.0	33.0	39.0
4	37.60140442882142	39.0	35.0	40.0	34.0	41.0
5	38.23382145553482	40.0	35.0	41.0	34.0	41.0
6	38.27342531828935	40.0	35.0	41.0	35.0	41.0
7	38.297585614055464	40.0	35.0	41.0	35.0	41.0
8	38.22101317864199	40.0	35.0	41.0	34.0	41.0
9	38.228007928881134	40.0	35.0	41.0	34.0	41.0
10	38.22377502840744	40.0	35.0	41.0	34.0	41.0
11	38.10529654957797	40.0	35.0	41.0	34.0	41.0
12	38.06363431158662	40.0	35.0	41.0	34.0	41.0
13	37.99062307900501	40.0	35.0	41.0	34.0	41.0
14	37.936436912470015	39.0	35.0	41.0	34.0	41.0
15	37.8838647926339	40.0	35.0	41.0	34.0	41.0
16	37.873140641213745	39.0	35.0	41.0	34.0	41.0
17	37.863113389793924	39.0	35.0	41.0	34.0	41.0
18	37.823388446143106	39.0	35.0	41.0	34.0	41.0
19	37.80914308693828	39.0	35.0	41.0	34.0	41.0
20	37.70447002179456	39.0	35.0	41.0	34.0	41.0
21	37.59447104084645	39.0	35.0	41.0	34.0	41.0
22	37.52833128609827	39.0	35.0	41.0	33.0	41.0
23	37.43709600893259	38.0	35.0	41.0	33.0	41.0
24	37.28608238103117	38.0	35.0	41.0	33.0	41.0
25	37.201553150536725	38.0	35.0	40.0	33.0	41.0
26	37.06269274342483	38.0	35.0	40.0	33.0	41.0
27	36.9973717061264	38.0	35.0	40.0	33.0	41.0
28	36.82136173952722	38.0	35.0	40.0	33.0	41.0
29	36.7819339635461	38.0	35.0	40.0	32.0	41.0
30	36.76626822547877	38.0	35.0	40.0	33.0	41.0
31	36.69986777097587	38.0	35.0	40.0	32.0	41.0
32	36.68842652388759	38.0	35.0	40.0	32.0	41.0
33	36.565106756173655	37.0	35.0	40.0	32.0	41.0
34	36.53475719857332	37.0	35.0	40.0	32.0	41.0
35	36.43133922301105	37.0	34.0	40.0	31.0	41.0
36	36.349537719972574	37.0	34.0	40.0	31.0	41.0
37	36.29304687928923	36.0	34.0	40.0	31.0	41.0
38	36.09444514154764	36.0	34.0	40.0	31.0	41.0
39	36.08357470359169	36.0	34.0	40.0	31.0	41.0
40	35.82342868139069	36.0	34.0	40.0	31.0	41.0
41	35.69616943013839	36.0	34.0	39.0	30.0	41.0
42	35.50316520888765	35.0	34.0	39.0	30.0	41.0
43	35.409001279379865	35.0	34.0	39.0	30.0	41.0
44	35.1753712786087	35.0	33.0	39.0	29.0	41.0
45	34.94611905339381	35.0	33.0	39.0	29.0	41.0
46	34.695732940292665	35.0	33.0	39.0	28.0	41.0
47	34.80156801906002	35.0	33.0	38.0	29.0	40.0
48	35.6633304904292	36.0	34.0	39.0	31.0	40.0
49	36.435484575150845	37.0	35.0	39.0	32.0	41.0
50	36.77428857419251	38.0	35.0	40.0	32.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	4.0
21	26.0
22	83.0
23	185.0
24	379.0
25	883.0
26	1727.0
27	3371.0
28	6520.0
29	11994.0
30	22467.0
31	42150.0
32	79739.0
33	139402.0
34	231953.0
35	115587.0
36	157160.0
37	254267.0
38	230581.0
39	232002.0
40	294746.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	48.91591507024336	16.18725571518267	34.89682921457398
2	26.291538691646956	23.52322397336001	30.44691451907873	19.7383228159143
3	24.69792781825374	24.386897841691933	22.10361894910548	28.811555390948847
4	18.814601589063493	27.578064305461353	27.606992230003296	26.00034187547186
5	15.947668946201732	31.020596901424806	27.7813815932931	25.25035255908035
6	21.128725976947514	30.257239377479827	25.020791945764525	23.593242699808133
7	24.44815053040007	27.67843543758417	21.388529420466288	26.484884611549475
8	20.68291817013345	26.690612559759725	27.860221145217086	24.76624812488974
9	21.4910920620241	30.92488272685136	25.963962818850927	21.620062392273613
10	29.328861193079653	22.62344498708653	26.455299234177026	21.59239458565679
11	23.75826336026333	24.826569422088003	24.633935742751856	26.781231474896806
12	21.6008866847174	26.7729585267797	24.88831520041902	26.73783958808389
13	22.36391548224713	27.01320274859113	24.382131308670818	26.24075046049092
14	18.856788145687165	26.757179658847726	24.98863154480596	29.39740065065915
15	19.63921180171661	32.752437232430395	26.89935383344309	20.708997132409905
16	19.54004599978304	36.41779155019707	21.566151260172713	22.476011189847174
17	21.230466802467202	36.68811423900383	19.539717273367792	22.54170168516118
18	21.380256472349178	30.01951539151864	21.46572534031402	27.13450279581816
19	23.478900695037215	24.86755064852243	23.607323147927982	28.046225508512368
20	23.096153572215165	25.485775460134803	24.87450869097854	26.54356227667149
21	22.048118972664206	33.42824395444728	22.17270628404373	22.350930788844778
22	23.867674468805507	28.570489353099287	21.340973665726874	26.22086251236833
23	26.097370955706307	25.713856804582008	25.903805884860287	22.2849663548514
24	21.55952194413185	29.903091452784476	22.38495397282309	26.15243263026058
25	21.50623761209027	26.65101433499442	24.710319851835365	27.132428201079943
26	22.380352198078718	33.55830689519857	25.053264250542618	19.008076656180094
27	23.931920941455758	28.374752760768363	21.985577850934686	25.707748446841194
28	23.25264481730446	25.980686905105767	22.891816772856473	27.874851504733307
29	24.493651821788994	29.19601444023699	24.86201828130379	21.44831545667023
30	26.518972734085384	28.42408199435249	26.759291183099638	18.297654088462494
31	23.711746489016924	30.228303925099027	23.416579042131797	22.64337054375225
32	21.19471581383377	28.871487238231275	25.4826098147474	24.451187133187556
33	20.48216759963121	26.400570814315433	30.850131463628394	22.26713012242496
34	21.133503802687592	31.053716631093515	25.926134642613114	21.88664492360578
35	20.602187088740564	27.183577359559514	23.391403903239322	28.8228316484606
36	20.72124588548515	28.14651679790571	25.42086033058587	25.711376986023264
37	19.57966423744471	32.48250888579633	21.914617582538245	26.023209294220713
38	24.142558069863348	27.048737919685305	23.27765646848056	25.531047541970782
39	21.371413870073148	30.834134545700508	25.10780131221676	22.686650272009583
40	18.921957172539955	28.616231173772505	25.540397680077753	26.921413973609788
41	21.059657080515805	32.3755577724485	22.97764487340777	23.58714027362793
42	19.854627496922284	34.89610221992772	22.20864689646956	23.040623386680434
43	20.159972753481846	26.074716789535728	24.114173970526505	29.651136486455915
44	20.434461757804684	27.472011354373148	23.53909274389416	28.554434143928002
45	19.810489126588617	26.56120420686381	29.946112182576844	23.68219448397073
46	18.45613151935491	29.98067481417942	27.41412336772439	24.149070298741275
47	21.763605889767707	30.7014992219929	25.099109331929686	22.435785556309707
48	22.901230732735005	28.5569531598597	23.570424006913342	24.971392100491954
49	18.269692550841317	28.845559407280636	23.300647031600636	29.584101010277415
50	20.443247165045413	32.52337829848982	25.64861342505509	21.384761111409684
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	46211.0
1	39765.5
2	24575.5
3	14888.0
4	12487.0
5	8413.0
6	4262.5
7	2847.5
8	2953.0
9	2909.0
10	2685.0
11	2372.5
12	2218.0
13	2064.5
14	2047.0
15	2232.5
16	2408.0
17	2950.5
18	3874.0
19	4254.5
20	4074.5
21	4649.5
22	5892.0
23	7136.0
24	8569.0
25	10166.5
26	12345.5
27	15231.5
28	17985.0
29	20730.0
30	24598.0
31	32193.0
32	43546.5
33	53271.5
34	70474.0
35	88333.0
36	84594.5
37	73777.5
38	71310.5
39	73658.0
40	81268.0
41	94170.0
42	105549.0
43	113040.5
44	118889.0
45	124092.0
46	132019.5
47	136672.0
48	151531.5
49	181073.0
50	212370.5
51	223098.0
52	193753.5
53	157588.0
54	133934.0
55	120963.0
56	115950.0
57	109620.0
58	98091.0
59	87409.0
60	79434.0
61	68350.0
62	57889.0
63	47467.5
64	37810.5
65	30782.5
66	22477.5
67	14704.5
68	10281.5
69	8043.5
70	6464.0
71	5097.5
72	3865.0
73	2676.0
74	1777.0
75	1321.5
76	1101.0
77	605.0
78	282.5
79	237.5
80	218.5
81	210.0
82	182.5
83	113.0
84	63.0
85	40.0
86	19.0
87	13.0
88	9.5
89	9.0
90	13.0
91	15.5
92	14.0
93	14.0
94	7.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
24	9948.0
25	11066.0
26	11414.0
27	13280.0
28	13342.0
29	11421.0
30	19130.0
31	10629.0
32	12355.0
33	9109.0
34	9042.0
35	9905.0
36	8877.0
37	10085.0
38	9799.0
39	13700.0
40	14220.0
41	16320.0
42	20188.0
43	37105.0
44	98860.0
45	160230.0
46	212306.0
47	197650.0
48	312630.0
49	274903.0
50	297712.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	10.96414441188375
#Duplication Level	Percentage of deduplicated	Percentage of total
1	67.62545205547458	7.414552222551441
2	11.883190684276999	2.6057803747272934
3	4.409489863315384	1.4503885093238222
4	2.2423769534782996	0.9834297897526403
5	1.4340911107166574	0.78617910188481
6	0.9764848304362636	0.6423792418150206
7	0.7610824428099613	0.5841232468622368
8	0.6088589552433809	0.5340494009405674
9	0.49037455934459845	0.4838883736111231
>10	5.205883667400222	13.737739611537425
>50	2.4928278077223127	19.57709166694329
>100	1.7697906093260516	31.95902816255401
>500	0.05856707792595237	4.389012547608574
>1k	0.03620510271786146	8.048789945323866
>5k	0.003194567886870129	2.356237460386046
>10k+	0.0021297119245800864	4.447330344177849
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGC	31256	1.7124454725058704	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTA	21002	1.1506520288446473	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCT	12325	0.6752588446581409	No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATCAT	11601	0.6355925238847135	No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACACA	8318	0.45572438700741713	No Hit
CGGCAAGTTTGAATTTCGTGGAGGCTCGGG	7800	0.427344339824219	No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATC	6826	0.3739810850820665	No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAGAA	6718	0.36806400960757735	No Hit
AGCTTTAATTTATTAATGCAAACAGTACCTAACAAACCCACAGGTCCT	5458	0.2990314624052035	No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACA	5243	0.28725209919210004	No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCTA	4686	0.2567353303097808	No Hit
TAGCTGTTAGTTGGTAGAGGGAAATTCAGGCTACCGTCGCGAAACCTG	4456	0.24413415105855382	No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTA	4424	0.24238094351055703	No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAG	3874	0.2122476887793621	No Hit
AGGTTTTCTGTTTCCTGTGATACCTTGGGCTGGAATCTGAAGCAATGAA	3329	0.1823883727275417	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACC	3255	0.1783340802727991	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAA	3202	0.1754303302714294	No Hit
ACAACCTTAGCCAAACCATTTACCCAAATAAAGTATAGGCGATAGAAAT	3155	0.1728553066853091	No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAA	3119	0.1708829481938127	No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTC	3013	0.16507544819107334	No Hit
AGCTTTAATTTATTAATGCAAACAGTACCTAACAAACCCACAGGTC	3007	0.16474672177582392	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAA	2972	0.16282915102020243	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAA	2898	0.15877485856545984	No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCC	2796	0.15318650950622006	No Hit
AACACAAAGCACCCAACTTACACTTAGGAGATTTCAACTTAACTTGACCG	2722	0.14913221705147747	No Hit
CAAACAGCCGGGGCTCCAGCGGGAGAACGATAATGCAAAGTGCTATGT	2643	0.14480398591736038	No Hit
CTGATGTTCGCCGACCGTTGACTATTCTCTACAAACCACAAAGACATTGG	2525	0.13833903308412218	No Hit
CACTTAGGAGATTTCAACTTAACTTGACCGCTCTGACCAAAAAAAAAAAA	2360	0.12929905666476368	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA	2348	0.1286416038342649	No Hit
AGCTTTAATTTATTAATGCAAACAGTACCTAACAAACCCACAGG	2311	0.1266144576068936	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2309	0.12650488213514383	No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAGAAT	2297	0.12584742930464501	No Hit
CCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCTAG	2261	0.12387507081314862	No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2239	0.12266974062390082	No Hit
TAGCTGTTAGTTGGTAGAGGGAAATTCAGGCTACCGTCGCGAAACC	2174	0.11910853779203232	No Hit
TTGAGTTCCAGCCAGGAGGACCAGGGGCTGGG	2149	0.11773884439515983	No Hit
ACAACCTTAGCCAAACCATTTACCCAAATAAAGTATAGGCGATAGAA	2099	0.11499945760141483	No Hit
CGGCAAGTTTGAATTTCGTGGAGGCTCGGGTTGTGAGGG	2060	0.11286273590229373	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTT	1981	0.10853450476817665	No Hit
ATAGGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACATGC	1954	0.10705523589955436	No Hit
AGGTTTTCTGTTTCCTGTGATACCTTGGGCTGGAATCTGAAGCAATG	1898	0.10398712269055997	No Hit
AGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTACTTTGAAAAAATTAGAGT	1890	0.10354882080356076	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.05511646229014928	0.0	0.0	0.0	0.0
2	0.09308436325145489	0.0	0.0	0.0	0.0
3	0.12250537741627612	0.0	0.0	0.0	0.0
4	0.14770773591873007	0.0	0.0	0.0	0.0
5	0.17192391517543582	0.0	0.0	0.0	0.0
6	0.20660455198424743	0.0	0.0	0.0	0.0
7	0.23279308973244958	0.0	0.0	0.0	0.0
8	0.2644604010681417	0.0	0.0	0.0	0.0
9	0.2937170520253382	0.0	0.0	0.0	0.0
10	0.32001516524529017	0.0	0.0	0.0	0.0
11	0.3470255190316158	0.0	0.0	0.0	0.0
12	0.3765013209323119	0.0	0.0	0.0	0.0
13	0.401648891698891	0.0	0.0	0.0	0.0
14	0.42646773605022065	0.0	0.0	0.0	0.0
15	0.4523275473831734	0.0	0.0	0.0	0.0
16	0.4791187502259994	0.0	0.0	0.0	0.0
17	0.5066769813710741	0.0	0.0	0.0	0.0
18	0.5416315568592601	0.0	0.0	0.0	0.0
19	0.5665051889464647	0.0	0.0	0.0	0.0
20	0.5898447644291721	0.0	0.0	0.0	0.0
21	0.6106093163257591	0.0	0.0	0.0	0.0
22	0.6312095050147215	0.0	0.0	0.0	0.0
23	0.6546586559691786	0.0	0.0	0.0	0.0
24	0.6776147173007616	0.0	0.0	0.0	0.0
25	0.7002968399529702	0.0	0.0	0.0	0.0
26	0.7285673116644186	0.0	0.0	0.0	0.0
27	0.7516329484677514	0.0	0.0	0.0	0.0
28	0.7765065805549559	0.0	0.0	0.0	0.0
29	0.8034621466054067	0.0	0.0	0.0	0.0
30	0.827952264541487	0.0	0.0	0.0	0.0
31	0.8525519579493169	0.0	0.0	0.0	0.0
32	0.8808224296607653	0.0	0.0	0.0	0.0
33	0.9066274532578431	0.0	0.0	0.0	0.0
34	0.9344596230822922	0.0	0.0	0.0	0.0
35	0.9562103542246275	0.0	0.0	0.0	0.0
36	0.975660000460217	0.0	0.0	0.0	0.0
37	0.9922058966944367	0.0	0.0	0.0	0.0
38	1.0039304721716653	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGCGAT	20	4.8163027E-5	183.42805	44
GTCTTAT	15	0.004991222	163.04715	44
CTTAACG	25	1.17202006E-4	146.74245	44
GGTCGAG	50	5.966285E-10	146.74245	44
AGCGATA	55	1.1586963E-9	133.40222	44
CTATCAT	2205	0.0	133.09972	44
CACACGT	15	2.1072538E-4	126.65766	43
AGGTACG	30	2.4223758E-4	122.285355	44
GAACACA	1790	0.0	107.939026	44
TCGCAAA	130	0.0	102.30042	43
AATCGCA	195	0.0	100.33671	44
CTTCGTT	85	0.0	96.85585	43
ACTTCTA	1140	0.0	96.54108	44
AATGTGC	90	3.255991E-10	95.11084	44
ACGTTAA	20	6.618082E-4	94.99325	43
GGTCCGT	20	6.618082E-4	94.99325	43
AGGTCGA	40	1.2314558E-8	94.99325	43
ACATTGG	755	0.0	89.08206	44
GACACGA	55	2.4165241E-5	88.934814	44
TTACGAG	65	0.0	87.68608	43
>>END_MODULE