FastQCFastQC Report
Tue 24 May 2016
ERR841053.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR841053.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6558049
Sequences flagged as poor quality0
Sequence length24-50
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGC1309361.996569406541488No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTA867651.3230306757390804No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCT494450.7539589899374036No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCTA271770.41440678470075476No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATCAT199780.30463328346586005No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACACA191730.2923582913149932No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAGAA186890.2849780475870186No Hit
CGGCAAGTTTGAATTTCGTGGAGGCTCGGG177570.2707665038794312No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTC174760.2664816929547187No Hit
AGCTTTAATTTATTAATGCAAACAGTACCTAACAAACCCACAGGTCCT174300.265780264831812No Hit
ATAGGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACATGC172280.26270008046600446No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACC137690.20995573531091336No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACA124350.18961431974661977No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAG114690.17488432916557958No Hit
ACAACCTTAGCCAAACCATTTACCCAAATAAAGTATAGGCGATAGAAAT112740.17191088386195344No Hit
TAGCTGTTAGTTGGTAGAGGGAAATTCAGGCTACCGTCGCGAAACCTG108980.1661774713790641No Hit
AGCTTTAATTTATTAATGCAAACAGTACCTAACAAACCCACAGGTC105890.16146570420562578No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAGAAT100090.1526216104820199No Hit
AGGTTTTCTGTTTCCTGTGATACCTTGGGCTGGAATCTGAAGCAATGAAG97480.14864176830639722No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACT94270.143747019883505No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTT88140.13439972772390082No Hit
TAGCTGTTAGTTGGTAGAGGGAAATTCAGGCTACCGTCGCGAAACCTGC87230.13301211991554193No Hit
CGCAATAGATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAG82580.12592159649920273No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCC81540.12433575900393547No Hit
CCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCTAG80170.12224672307266993No Hit
AGGTTTTCTGTTTCCTGTGATACCTTGGGCTGGAATCTGAAGCAATGAA77110.11758070121159508No Hit
AAGCAGTGGTATCAACGCAGAGTGCAG73490.11206076685306865No Hit
AAGCAGTGGTATCAACGCAGAGTGCAGTGCT73020.11134409029270748No Hit
ACAACCTTAGCCAAACCATTTACCCAAATAAAGTATAGGCGATAGAA72650.11077989810689125No Hit
AGCTTTAATTTATTAATGCAAACAGTACCTAACAAACCCACAGG70070.10684580124363206No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAA70040.10680005593126857No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAG69010.1052294668734558No Hit
AACACAAAGCACCCAACTTACACTTAGGAGATTTCAACTTAACTTGACCG68300.10414682781418681No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGCC65780.10030422157565459No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGTAC301.0966687E-8177.835344
ACTTCTA54250.0117.0270944
CTATCAT48600.098.3582944
CCTTAGC203950.084.4495343
CGTGCGC2350.081.7285644
CCAGAAT29050.079.337244
AATCGCA7600.078.6219244
GAACACA56700.073.956944
TCCGGGC10700.072.8948843
TCGCAAA4300.070.38998443
GTACGTA1251.6916601E-1068.2887544
AACGACA5900.068.07271643
TCCAGAA52250.068.0661143
TGAACAC36100.064.4954143
AATGAAG35800.063.18617644
CCGGGCT13600.062.765444
TCGTGCG5500.062.4403543
CCCTATC47500.061.90883342
CAATGAA36650.061.14525643
GGTACGC200.00446000158.73507742
Produced by FastQC (version 0.11.5)