##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR841053.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 6558049 Sequences flagged as poor quality 0 Sequence length 24-50 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 37.319516063390196 39.0 37.0 39.0 34.0 39.0 2 37.85720219534804 39.0 38.0 39.0 35.0 39.0 3 38.048105770481435 39.0 38.0 39.0 35.0 39.0 4 38.5243710438882 39.0 38.0 40.0 35.0 41.0 5 39.2094070965313 40.0 38.0 41.0 36.0 41.0 6 39.13256915280749 40.0 38.0 41.0 35.0 41.0 7 39.13358378383571 40.0 38.0 41.0 35.0 41.0 8 39.130780816062824 40.0 38.0 41.0 35.0 41.0 9 39.18687417553605 40.0 39.0 41.0 35.0 41.0 10 39.10120677658858 40.0 39.0 41.0 35.0 41.0 11 39.03303512980766 40.0 38.0 41.0 35.0 41.0 12 39.00493820646964 40.0 38.0 41.0 35.0 41.0 13 38.9196570504429 40.0 38.0 41.0 35.0 41.0 14 38.86977666681051 40.0 38.0 41.0 35.0 41.0 15 38.83342103726276 40.0 38.0 41.0 35.0 41.0 16 38.79122662852931 40.0 38.0 41.0 35.0 41.0 17 38.807098269622564 40.0 38.0 41.0 35.0 41.0 18 38.780968089747425 40.0 38.0 41.0 35.0 41.0 19 38.77934901065851 40.0 38.0 41.0 35.0 41.0 20 38.72788507679647 40.0 38.0 41.0 35.0 41.0 21 38.61334476152892 40.0 38.0 41.0 35.0 41.0 22 38.54569735602769 40.0 38.0 41.0 35.0 41.0 23 38.39145544658175 40.0 38.0 41.0 34.0 41.0 24 38.29035937364908 40.0 37.0 41.0 34.0 41.0 25 38.19808170069495 40.0 37.0 41.0 34.0 41.0 26 38.077535129197216 40.0 37.0 41.0 34.0 41.0 27 37.9860471766787 39.0 37.0 41.0 33.0 41.0 28 37.81148956812538 39.0 36.0 41.0 33.0 41.0 29 37.80314408637003 39.0 36.0 41.0 33.0 41.0 30 37.75124298196902 39.0 36.0 41.0 33.0 41.0 31 37.74215646011652 39.0 36.0 41.0 33.0 41.0 32 37.682238881646704 39.0 36.0 41.0 33.0 41.0 33 37.555463956956125 39.0 36.0 41.0 33.0 41.0 34 37.53013923583177 39.0 36.0 40.0 33.0 41.0 35 37.38795243759296 38.0 35.0 40.0 33.0 41.0 36 37.34732064025952 38.0 35.0 40.0 32.0 41.0 37 37.27845131632828 38.0 35.0 40.0 32.0 41.0 38 37.060467150826355 38.0 35.0 40.0 32.0 41.0 39 37.03398420314017 38.0 35.0 40.0 32.0 41.0 40 36.79653959484904 38.0 35.0 40.0 31.0 41.0 41 36.7259552493595 38.0 35.0 40.0 31.0 41.0 42 36.56588493768353 38.0 34.0 40.0 31.0 41.0 43 36.429756533341205 38.0 34.0 40.0 31.0 41.0 44 36.161226750048414 37.0 34.0 40.0 30.0 41.0 45 35.91647641263388 37.0 34.0 40.0 29.0 41.0 46 35.575925358233256 37.0 33.0 40.0 28.0 41.0 47 35.66137423744353 37.0 34.0 40.0 29.0 41.0 48 36.54503757188483 38.0 35.0 40.0 31.0 41.0 49 37.28983183061718 38.0 35.0 40.0 33.0 41.0 50 36.93219705134989 38.0 35.0 40.0 32.0 41.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 18 1.0 19 2.0 20 14.0 21 45.0 22 133.0 23 233.0 24 494.0 25 1004.0 26 1702.0 27 3620.0 28 7925.0 29 17582.0 30 36404.0 31 71105.0 32 131113.0 33 223481.0 34 337937.0 35 387415.0 36 645061.0 37 1074826.0 38 1124386.0 39 1072953.0 40 1420613.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 0.0 45.82335386637093 17.783459684427488 36.393186449201586 2 24.102961109317725 23.265821893066065 32.414015204826924 20.21720179278929 3 24.077602957830905 27.33302236686551 22.333532427098366 26.25584224820522 4 21.206017216400795 27.08380190510928 26.579124370677924 25.131056507812005 5 19.292124837737564 29.8938297045356 26.385789432192407 24.428256025534424 6 22.49188745006327 26.994735781937585 25.15574372805083 25.35763303994831 7 22.68854654791387 24.568251929804124 24.495837100332736 28.24736442194927 8 21.387778590858346 22.526333670272972 32.11763132602394 23.968256412844735 9 24.782843190101204 29.277457365750088 24.698214362228764 21.24148508191994 10 30.963690573217733 20.940755398442434 27.221708773447713 20.873845254892117 11 24.6066932406269 24.47102789259428 24.6698217716885 26.252457095090325 12 21.931339640798658 24.272645721311324 26.89867062597428 26.89734401191574 13 22.027374299887054 25.111614750057527 24.105294120248264 28.75571682980716 14 19.57281807439987 27.09479602851397 23.966563836287285 29.365822060798873 15 18.36996033423965 34.90137081927872 25.471874333357373 21.25679451312425 16 19.54149778386834 34.86477456938794 22.26665278042296 23.32707486632076 17 22.782095711697185 35.10425127961075 19.940366410802966 22.173286597889096 18 20.470203867034233 27.590751456721353 21.704046432101986 30.234998244142425 19 21.840809667631333 25.861685388444034 24.02392845799109 28.273576485933543 20 23.03139241564069 24.30250216184722 25.97460006779455 26.69150535471754 21 21.2116438898215 33.177412977548656 23.720713279208496 21.890229853421346 22 24.801796997857135 27.7399116719012 21.37818732369947 26.080104006542186 23 24.47790493788625 25.342338857181456 28.035197663207455 22.14455854172483 24 22.48694695632802 28.473605488461583 22.14783695577755 26.89161059943285 25 22.02701254203027 25.306949203926337 25.479678334930433 27.186359919112963 26 22.411470785952698 34.09827637382909 24.652206383898807 18.8380464563194 27 24.212690550427634 28.161611579907674 21.72952237900068 25.896175490664007 28 23.797854353061556 26.415805662661125 23.11126605023771 26.675073934039606 29 24.56493362505269 27.345564314255366 26.122576150749303 21.966925909942642 30 24.617897550488443 27.54759183694098 27.139492585383547 20.695018027187032 31 22.701870822371077 31.16990263334747 23.751649617103972 22.37657692717748 32 21.769646002467958 27.803161505148367 25.325260586217336 25.101931906166346 33 20.863720235480187 25.40022522056885 31.932606976539844 21.803447567411112 34 20.590234686218384 31.629876737524754 25.849327530784766 21.9305610454721 35 19.726092894836366 26.56167024939303 25.450077957774127 28.262158897996482 36 20.86649579899683 27.39271797655635 24.76174009165377 26.979046132793044 37 18.876926938166257 32.8068570598259 23.83848724621217 24.477728755795674 38 25.652259619464502 27.007986459338575 22.814277413171688 24.52547650802524 39 21.630928518416173 31.396313175542833 24.950773605335634 22.021984700705357 40 18.928832986663732 26.76978951890468 26.35802475365601 27.94335274077558 41 20.33823182183334 31.763974906639454 24.89456229102436 23.003230980502845 42 19.409677880508053 33.515974645222585 24.766873259629282 22.30747421464008 43 19.873459714739504 26.074493223202495 24.97112977019349 29.080917291864512 44 19.713377011662857 27.393351375223656 22.822147027159744 30.07112458595374 45 18.819767267520184 26.169909592468272 30.817247658737017 24.193075481274523 46 18.721027536009743 27.765366021384214 29.524447684895584 23.989158757710456 47 20.75671852899576 31.23011574010587 25.23408083784239 22.779084893055984 48 24.245455707712363 27.580684860961924 24.21988603573302 23.953973395592694 49 18.518278169538384 27.727035334016353 23.52384529394639 30.23084120249887 50 20.214240921828765 30.504713006329737 25.137666816908034 24.14337925493347 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 73518.0 1 63618.5 2 52919.5 3 49421.0 4 42134.5 5 28819.0 6 15595.5 7 11461.5 8 11531.0 9 10580.0 10 9324.0 11 8920.5 12 9234.0 13 9294.0 14 9277.0 15 9159.0 16 8775.0 17 8953.5 18 10734.5 19 13146.0 20 14905.5 21 17596.5 22 20782.5 23 24274.5 24 28508.5 25 33281.5 26 39344.5 27 47741.5 28 57972.0 29 67598.5 30 79414.5 31 115263.0 32 175863.5 33 208610.5 34 252566.0 35 314701.5 36 310395.0 37 292561.0 38 294527.5 39 295476.0 40 313197.5 41 345461.5 42 387665.5 43 428476.0 44 453213.0 45 466167.0 46 481695.0 47 497081.0 48 576873.5 49 685195.0 50 797913.5 51 868873.0 52 755698.0 53 588447.5 54 493164.5 55 439723.0 56 395503.5 57 352546.0 58 314640.0 59 278470.0 60 251460.0 61 222390.5 62 185086.5 63 145574.5 64 114980.0 65 96226.0 66 73950.5 67 52880.0 68 38243.5 69 28394.5 70 21952.0 71 16738.0 72 12953.5 73 9636.5 74 6389.0 75 4264.5 76 3151.0 77 2077.5 78 1315.5 79 877.5 80 664.5 81 417.0 82 235.5 83 464.5 84 695.5 85 678.0 86 640.0 87 319.0 88 21.5 89 21.0 90 13.5 91 6.0 92 5.0 93 4.0 94 12.0 95 19.5 96 19.0 97 19.0 98 9.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 >>END_MODULE >>Sequence Length Distribution warn #Length Count 24 36856.0 25 32431.0 26 33581.0 27 44061.0 28 43356.0 29 36216.0 30 56607.0 31 40632.0 32 43045.0 33 33510.0 34 33926.0 35 33735.0 36 33853.0 37 34673.0 38 32402.0 39 44023.0 40 49718.0 41 47532.0 42 57325.0 43 104910.0 44 283231.0 45 574566.0 46 744228.0 47 735916.0 48 1097901.0 49 1025119.0 50 1224696.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 9.790874608068282 #Duplication Level Percentage of deduplicated Percentage of total 1 70.05039323352992 6.858546163953664 2 12.968859842839798 2.5395296106171314 3 4.59642312464334 1.3500900737702504 4 2.1265976625887664 0.8328500422487086 5 1.2223667701359722 0.5984019885735364 6 0.7936987962590548 0.46626032344482843 7 0.5546528299877838 0.3801375416594424 8 0.43891541045235255 0.34378925978302444 9 0.3357116660719526 0.29582197442785607 >10 3.1782090369530995 7.097514459028336 >50 1.5665738032418948 11.41059921595006 >100 2.013111575336934 33.580069231100516 >500 0.08287169270371131 5.710166626644991 >1k 0.059878390681872326 11.750750412167136 >5k 0.00742492044455217 5.001680948575683 >10k+ 0.004311244129094808 11.783792128054852 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGC 130936 1.996569406541488 No Hit CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTA 86765 1.3230306757390804 No Hit CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCT 49445 0.7539589899374036 No Hit TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCTA 27177 0.41440678470075476 No Hit ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATCAT 19978 0.30463328346586005 No Hit CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACACA 19173 0.2923582913149932 No Hit ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAGAA 18689 0.2849780475870186 No Hit CGGCAAGTTTGAATTTCGTGGAGGCTCGGG 17757 0.2707665038794312 No Hit TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTC 17476 0.2664816929547187 No Hit AGCTTTAATTTATTAATGCAAACAGTACCTAACAAACCCACAGGTCCT 17430 0.265780264831812 No Hit ATAGGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACATGC 17228 0.26270008046600446 No Hit CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACC 13769 0.20995573531091336 No Hit CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACA 12435 0.18961431974661977 No Hit ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAG 11469 0.17488432916557958 No Hit ACAACCTTAGCCAAACCATTTACCCAAATAAAGTATAGGCGATAGAAAT 11274 0.17191088386195344 No Hit TAGCTGTTAGTTGGTAGAGGGAAATTCAGGCTACCGTCGCGAAACCTG 10898 0.1661774713790641 No Hit AGCTTTAATTTATTAATGCAAACAGTACCTAACAAACCCACAGGTC 10589 0.16146570420562578 No Hit ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAGAAT 10009 0.1526216104820199 No Hit AGGTTTTCTGTTTCCTGTGATACCTTGGGCTGGAATCTGAAGCAATGAAG 9748 0.14864176830639722 No Hit TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACT 9427 0.143747019883505 No Hit CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTT 8814 0.13439972772390082 No Hit TAGCTGTTAGTTGGTAGAGGGAAATTCAGGCTACCGTCGCGAAACCTGC 8723 0.13301211991554193 No Hit CGCAATAGATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAG 8258 0.12592159649920273 No Hit ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCC 8154 0.12433575900393547 No Hit CCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCTAG 8017 0.12224672307266993 No Hit AGGTTTTCTGTTTCCTGTGATACCTTGGGCTGGAATCTGAAGCAATGAA 7711 0.11758070121159508 No Hit AAGCAGTGGTATCAACGCAGAGTGCAG 7349 0.11206076685306865 No Hit AAGCAGTGGTATCAACGCAGAGTGCAGTGCT 7302 0.11134409029270748 No Hit ACAACCTTAGCCAAACCATTTACCCAAATAAAGTATAGGCGATAGAA 7265 0.11077989810689125 No Hit AGCTTTAATTTATTAATGCAAACAGTACCTAACAAACCCACAGG 7007 0.10684580124363206 No Hit CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAA 7004 0.10680005593126857 No Hit CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAG 6901 0.1052294668734558 No Hit AACACAAAGCACCCAACTTACACTTAGGAGATTTCAACTTAACTTGACCG 6830 0.10414682781418681 No Hit CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGCC 6578 0.10030422157565459 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.03842606238532222 0.0 0.0 0.0 0.0 2 0.09165835753895708 0.0 0.0 0.0 0.0 3 0.11588812465414637 0.0 0.0 0.0 0.0 4 0.13346957303917675 0.0 0.0 0.0 0.0 5 0.20094390877530802 0.0 0.0 0.0 0.0 6 0.23345357742828698 0.0 0.0 0.0 0.0 7 0.2617394289063714 0.0 0.0 0.0 0.0 8 0.2996165475433319 0.0 0.0 0.0 0.0 9 0.32190976310180053 0.0 0.0 0.0 0.0 10 0.34205294897918576 0.0 0.0 0.0 0.0 11 0.37293103482453394 0.0 0.0 0.0 0.0 12 0.3982739378739012 0.0 0.0 0.0 0.0 13 0.4223817174894546 0.0 0.0 0.0 0.0 14 0.4478923533508213 0.0 0.0 0.0 0.0 15 0.48612018604923507 0.0 0.0 0.0 0.0 16 0.5096790219164267 0.0 0.0 0.0 0.0 17 0.5805080138925464 0.0 0.0 0.0 0.0 18 0.6104254481782615 0.0 0.0 0.0 0.0 19 0.6922028182467073 0.0 0.0 0.0 0.0 20 0.7167832994233498 0.0 0.0 0.0 0.0 21 0.7589909742973863 0.0 0.0 0.0 0.0 22 0.780262544546404 0.0 0.0 0.0 0.0 23 0.8074810054026739 0.0 0.0 0.0 0.0 24 0.8380388740614777 0.0 0.0 0.0 0.0 25 0.8614604739915789 0.0 0.0 0.0 0.0 26 0.9704715533537489 0.0 0.0 0.0 0.0 27 0.9965311329634774 0.0 0.0 0.0 0.0 28 1.0360398344080686 0.0 0.0 0.0 0.0 29 1.0724683514868523 0.0 0.0 0.0 0.0 30 1.1017758482743878 0.0 0.0 0.0 0.0 31 1.126066609139395 0.0 0.0 0.0 0.0 32 1.1520651949993055 0.0 0.0 0.0 0.0 33 1.1752885652424983 0.0 0.0 0.0 0.0 34 1.199564077670051 0.0 0.0 0.0 0.0 35 1.2214455854172483 0.0 0.0 0.0 0.0 36 1.2407653556720908 0.0 0.0 0.0 0.0 37 1.2582248165574854 0.0 0.0 0.0 0.0 38 1.2703778212087162 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCGGTAC 30 1.0966687E-8 177.8353 44 ACTTCTA 5425 0.0 117.02709 44 CTATCAT 4860 0.0 98.35829 44 CCTTAGC 20395 0.0 84.44953 43 CGTGCGC 235 0.0 81.72856 44 CCAGAAT 2905 0.0 79.3372 44 AATCGCA 760 0.0 78.62192 44 GAACACA 5670 0.0 73.9569 44 TCCGGGC 1070 0.0 72.89488 43 TCGCAAA 430 0.0 70.389984 43 GTACGTA 125 1.6916601E-10 68.28875 44 AACGACA 590 0.0 68.072716 43 TCCAGAA 5225 0.0 68.06611 43 TGAACAC 3610 0.0 64.49541 43 AATGAAG 3580 0.0 63.186176 44 CCGGGCT 1360 0.0 62.7654 44 TCGTGCG 550 0.0 62.44035 43 CCCTATC 4750 0.0 61.908833 42 CAATGAA 3665 0.0 61.145256 43 GGTACGC 20 0.004460001 58.735077 42 >>END_MODULE