##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR841052.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	76128603
Sequences flagged as poor quality	0
Sequence length	24-50
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	37.35146490209468	39.0	37.0	39.0	34.0	39.0
2	37.90766951286365	39.0	38.0	39.0	35.0	39.0
3	38.091162673771905	39.0	38.0	39.0	35.0	39.0
4	38.54388194933775	39.0	38.0	40.0	35.0	41.0
5	39.28486106595178	40.0	39.0	41.0	36.0	41.0
6	39.27366833462056	40.0	39.0	41.0	36.0	41.0
7	39.29329890632565	40.0	39.0	41.0	36.0	41.0
8	39.225548864991	40.0	39.0	41.0	35.0	41.0
9	39.279727949296536	40.0	39.0	41.0	36.0	41.0
10	39.25644956337896	40.0	39.0	41.0	35.0	41.0
11	39.19085484860401	40.0	39.0	41.0	35.0	41.0
12	39.10940713045792	40.0	38.0	41.0	35.0	41.0
13	39.03693421249304	40.0	38.0	41.0	35.0	41.0
14	39.02568131980564	40.0	38.0	41.0	35.0	41.0
15	38.99303682217839	40.0	38.0	41.0	35.0	41.0
16	38.947738644304295	40.0	38.0	41.0	35.0	41.0
17	38.8985640259286	40.0	38.0	41.0	35.0	41.0
18	38.909452049185774	40.0	38.0	41.0	35.0	41.0
19	38.88322988141527	40.0	38.0	41.0	35.0	41.0
20	38.81811224094051	40.0	38.0	41.0	35.0	41.0
21	38.73189291283855	40.0	38.0	41.0	35.0	41.0
22	38.652586584309184	40.0	38.0	41.0	35.0	41.0
23	38.56253581850175	40.0	38.0	41.0	35.0	41.0
24	38.43764176258429	40.0	38.0	41.0	34.0	41.0
25	38.32029548060073	40.0	37.0	41.0	34.0	41.0
26	38.19506918929691	40.0	37.0	41.0	34.0	41.0
27	38.112038380102135	40.0	37.0	41.0	34.0	41.0
28	37.99789933623978	39.0	37.0	41.0	33.0	41.0
29	37.94869142947786	39.0	36.0	41.0	33.0	41.0
30	37.92365788927402	39.0	36.0	41.0	33.0	41.0
31	37.83819932647876	39.0	36.0	41.0	33.0	41.0
32	37.764396006261656	39.0	36.0	41.0	33.0	41.0
33	37.65176475673496	39.0	36.0	41.0	33.0	41.0
34	37.563377346141806	39.0	36.0	41.0	33.0	41.0
35	37.49660749153978	39.0	35.0	41.0	33.0	41.0
36	37.45455760504942	39.0	35.0	41.0	33.0	41.0
37	37.36674784962233	38.0	35.0	41.0	33.0	41.0
38	37.244041190674956	38.0	35.0	41.0	32.0	41.0
39	37.139193540668686	38.0	35.0	40.0	32.0	41.0
40	36.956356841817644	38.0	35.0	40.0	32.0	41.0
41	36.83388588349528	38.0	35.0	40.0	31.0	41.0
42	36.6883184958345	38.0	35.0	40.0	31.0	41.0
43	36.57719056330126	38.0	34.0	40.0	31.0	41.0
44	36.3922368128125	37.0	34.0	40.0	31.0	41.0
45	36.1830790064627	37.0	34.0	40.0	30.0	41.0
46	35.84079569136235	37.0	34.0	40.0	29.0	41.0
47	35.869777062075656	37.0	34.0	40.0	29.0	41.0
48	36.71071842225742	38.0	35.0	40.0	31.0	41.0
49	37.40720353408583	39.0	35.0	40.0	33.0	41.0
50	37.22368152678611	38.0	35.0	40.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	3.0
19	31.0
20	158.0
21	525.0
22	1356.0
23	2882.0
24	5887.0
25	11825.0
26	21086.0
27	43164.0
28	90741.0
29	190140.0
30	384776.0
31	755607.0
32	1390869.0
33	2439180.0
34	3839521.0
35	4001505.0
36	6830651.0
37	1.2179029E7
38	1.2824668E7
39	1.2730878E7
40	1.838412E7
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	57.40768683224097	19.07513132744601	23.51718184031303
2	27.728545077859895	24.612273523527026	30.092357008048605	17.566824390564477
3	25.693378348214267	21.748916107129933	27.254795415068894	25.302910129586902
4	21.22640159310424	24.973849841957563	30.311844813440224	23.487903751497974
5	20.26923572996604	26.895276142135433	27.77406673284153	25.061421395056993
6	22.949466181587493	29.806275310214218	26.148280955582493	21.095977552615803
7	27.332403564531454	27.80453097241256	24.09689167683794	20.76617378621804
8	23.369086386623962	26.44722903952408	27.32021103815605	22.863473535695906
9	24.275693066376117	26.851856193919648	26.87980758033876	21.992643159365475
10	24.91130436217252	24.99648785095925	27.216916091314587	22.87529169555364
11	25.334982174833815	26.222163829802575	27.061643309020134	21.381210686343476
12	24.801421878186837	26.073062183999358	26.77863798446426	22.346877953349544
13	23.554387304335535	25.799666913630347	27.24950305471913	23.39644272731499
14	20.993649390886628	27.803721815307707	27.643864685130236	23.55876410867542
15	21.044054886965416	29.73217438391717	26.87113541279616	22.35263531632125
16	22.76180347089779	31.830930878897124	23.978153125967648	21.42911252423744
17	22.68123979629575	31.67650797427611	22.99700547506435	22.645246754363797
18	23.831549884082335	28.723711112891433	24.223657433987064	23.22108156903917
19	24.11061582201896	26.857293046609566	26.206072637376522	22.826018493994958
20	24.8582113085669	25.8343857958355	26.642511750806726	22.664891144790875
21	25.17115281886888	27.919315687429595	25.1125795648713	21.796951928830218
22	25.1990358998181	29.193673762803712	24.194894263329644	21.41239607404854
23	26.023619532332674	28.4428114358016	24.788944307831315	20.74462472403441
24	25.843861866216038	28.59633454721348	24.431103773177078	21.128699813393396
25	23.810201016935146	28.791687453983666	25.933609364356215	21.464502164724976
26	24.230966806901687	30.675169474041358	25.718805155926518	19.375058563130438
27	25.40036468153643	29.341354051322945	23.716465068772223	21.541816198368398
28	24.937920558899098	28.86336208476928	24.714996066582287	21.483721289749337
29	25.429401138057955	29.361100257035055	24.70058600156816	20.50891260333883
30	26.305091461508546	29.356537900359776	25.25742300125608	19.080947636875603
31	23.785396866622115	29.52946343915146	25.029487596544858	21.655652097681568
32	23.52840380974703	29.357627647747908	26.77362717612739	20.340341366377668
33	23.47713124009589	28.646502437214373	26.83263900065778	21.043727322031955
34	22.90452841856772	28.54354304933709	27.028588198982366	21.523340333112824
35	23.339952022892973	27.82168252724319	26.662954283432384	22.175411166431452
36	23.394242507171406	29.0209421398727	25.4607889990892	22.12402635386669
37	22.52713776976874	30.502507162064163	26.114653516462727	20.85570155170437
38	23.03043106629802	29.969618436354445	24.97538052530586	22.02456997204168
39	22.68271585769111	29.559631373083032	26.36604245241895	21.391610316806908
40	21.953483996718067	29.387846698937032	27.120288121583368	21.538381182761537
41	22.33606204856557	29.632290204219235	26.985164144329705	21.046483602885484
42	22.516051127110423	30.349856835257818	25.574901744192413	21.559190293439347
43	21.875820804346837	29.094797098596377	26.5670135983794	22.46236849867739
44	21.43486572961202	29.40033167944324	25.56609570528386	23.598706885660885
45	21.756213901913778	28.682049064202715	27.001441361286428	22.560295672597082
46	22.315237446474878	29.7366002093315	26.46708906366368	21.481073280529948
47	22.805110434151686	29.42722675408057	26.84448229833582	20.923180513431923
48	22.69944249130319	29.081690690275053	26.199419365432576	22.01944745298918
49	21.234236735344474	29.813646044389923	25.181495365497263	23.770621854768333
50	21.02467905212361	31.07063795970974	26.960001519611552	20.944681468555096
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	999526.0
1	904388.0
2	667327.0
3	486022.5
4	388952.5
5	278336.0
6	183072.5
7	141588.5
8	138040.5
9	133834.0
10	134240.5
11	139081.0
12	146655.5
13	154308.5
14	170082.0
15	190857.0
16	214176.5
17	250897.0
18	298910.0
19	358348.0
20	418699.5
21	500718.0
22	593377.5
23	719046.0
24	860120.0
25	979097.0
26	1121628.0
27	1295088.0
28	1467616.5
29	1651948.5
30	1862075.5
31	2089009.5
32	2380649.0
33	2653188.5
34	3003898.5
35	3387898.0
36	3605496.0
37	3759819.0
38	3893887.5
39	4094683.5
40	4359831.5
41	4696482.0
42	5013450.5
43	5100369.5
44	5135244.0
45	5196854.0
46	5320129.5
47	5430854.0
48	5570381.5
49	5803249.0
50	6127838.0
51	6334204.0
52	6230600.0
53	5895282.0
54	5379414.5
55	5010690.0
56	4729132.0
57	4389052.0
58	3883585.0
59	3338793.0
60	3023354.5
61	2728472.5
62	2354293.0
63	1936069.0
64	1551081.0
65	1279629.5
66	984569.5
67	705167.5
68	512795.5
69	376555.0
70	286761.0
71	210634.5
72	153936.5
73	105750.0
74	67092.5
75	44778.5
76	34036.0
77	25983.0
78	18362.5
79	12677.0
80	9005.5
81	6298.0
82	4285.0
83	3153.0
84	2333.0
85	1769.0
86	1359.0
87	848.5
88	431.5
89	391.5
90	449.0
91	461.5
92	406.0
93	403.0
94	206.5
95	8.5
96	7.0
97	7.0
98	7.5
99	8.0
100	8.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
24	355236.0
25	363293.0
26	359935.0
27	428011.0
28	384784.0
29	340684.0
30	709956.0
31	348482.0
32	379680.0
33	331508.0
34	334877.0
35	347309.0
36	358498.0
37	362867.0
38	342947.0
39	518085.0
40	572211.0
41	502703.0
42	649344.0
43	1133136.0
44	3191771.0
45	6662785.0
46	9296328.0
47	7617850.0
48	1.2983561E7
49	1.1196027E7
50	1.6056735E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	7.736354896221121
#Duplication Level	Percentage of deduplicated	Percentage of total
1	65.98819441732023	5.1050809097322665
2	12.147777280677891	1.8795903248715222
3	4.058504146997051	0.9419408528686307
4	1.872950077477973	0.5795922600909778
5	1.072529085195891	0.41487328197973955
6	0.7715545713500211	0.35814119914593145
7	0.5326996983874223	0.2884807743884534
8	0.4398144880586913	0.27220487744974736
9	0.39028578764824656	0.27174504277782263
>10	5.588214926814033	11.441674388170256
>50	4.280776663767652	24.283862340772018
>100	2.7349474229472324	33.41905865630726
>500	0.07438007688357609	3.905836254397105
>1k	0.03922438217756175	5.983960854215324
>5k	0.004472442962924396	2.4141710071981284
>10k+	0.0036745313335883405	8.439786975634886
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGGCAAGTTTGAATTTCGTGGAGGCTCGGG	341466	0.44853837656787154	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGC	192654	0.2530638845428439	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTA	119644	0.15716037768353638	No Hit
CAAAGCACCCAACTTACACTTAGGAGATTTCAACTTAACTTGACCGCTCT	106815	0.14030863012158518	No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATCAT	101188	0.13291719013942763	No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAGAA	98204	0.12899750702111268	No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACACA	84295	0.11072710739221103	No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	81490	0.10704255271832584	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCT	77476	0.10176989586949337	No Hit
CGGCAAGTTTGAATTTCGTGGAGGCTCGGGTTGTGAGGG	76647	0.10068094905143603	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.050012739626917886	0.0	0.0	0.0	0.0
2	0.09233585962427289	0.0	0.0	0.0	0.0
3	0.11682599771336931	0.0	0.0	0.0	0.0
4	0.13855764567228432	0.0	0.0	0.0	0.0
5	0.16116412907248542	0.0	0.0	0.0	0.0
6	0.19761166509255398	0.0	0.0	0.0	0.0
7	0.2257889849889929	0.0	0.0	0.0	0.0
8	0.25490681866315085	0.0	0.0	0.0	0.0
9	0.2800038245808872	0.0	0.0	0.0	0.0
10	0.3036256950623408	0.0	0.0	0.0	0.0
11	0.32822223205645845	0.0	0.0	0.0	0.0
12	0.3528279640176768	1.3135667286578213E-6	0.0	0.0	0.0
13	0.37759526468652	1.3135667286578213E-6	0.0	0.0	0.0
14	0.40289981414738424	1.3135667286578213E-6	0.0	0.0	0.0
15	0.4307539966285734	1.3135667286578213E-6	0.0	0.0	0.0
16	0.45604803755560835	1.3135667286578213E-6	0.0	3.5466301673761176E-5	0.0
17	0.485750408424019	1.3135667286578213E-6	0.0	3.677986840241899E-5	1.3135667286578213E-6
18	0.5111731788904625	1.3135667286578213E-6	0.0	3.9801071878331983E-4	1.3135667286578213E-6
19	0.540657497681916	1.3135667286578213E-6	0.0	3.9801071878331983E-4	1.3135667286578213E-6
20	0.5660723867479874	1.3135667286578213E-6	0.0	3.9801071878331983E-4	1.3135667286578213E-6
21	0.5924054589573908	1.3135667286578213E-6	0.0	3.9801071878331983E-4	1.3135667286578213E-6
22	0.6175353040433436	1.3135667286578213E-6	0.0	3.9801071878331983E-4	1.3135667286578213E-6
23	0.6452266042501791	1.3135667286578213E-6	0.0	3.9801071878331983E-4	1.3135667286578213E-6
24	0.6711839963751863	1.3135667286578213E-6	0.0	3.9801071878331983E-4	2.6271334573156425E-6
25	0.699492672944491	2.6271334573156425E-6	0.0	3.9801071878331983E-4	2.6271334573156425E-6
26	0.734656854270661	2.6271334573156425E-6	0.0	3.9801071878331983E-4	2.6271334573156425E-6
27	0.7616375148772926	2.6271334573156425E-6	0.0	3.9801071878331983E-4	2.6271334573156425E-6
28	0.7907711113521944	2.6271334573156425E-6	0.0	3.9801071878331983E-4	2.6271334573156425E-6
29	0.8223479419423998	3.940700185973464E-6	0.0	3.9801071878331983E-4	2.6271334573156425E-6
30	0.8522617970541243	3.940700185973464E-6	0.0	3.9801071878331983E-4	2.6271334573156425E-6
31	0.8827194687915132	3.940700185973464E-6	0.0	3.9801071878331983E-4	2.6271334573156425E-6
32	0.9133636670043715	5.254266914631285E-6	0.0	3.9801071878331983E-4	2.6271334573156425E-6
33	0.9448130816218971	5.254266914631285E-6	0.0	3.9801071878331983E-4	2.6271334573156425E-6
34	0.9743328667150243	5.254266914631285E-6	0.0	3.9801071878331983E-4	2.6271334573156425E-6
35	1.001471155329095	5.254266914631285E-6	0.0	3.9801071878331983E-4	2.6271334573156425E-6
36	1.0245255649837683	5.254266914631285E-6	0.0	3.9801071878331983E-4	2.6271334573156425E-6
37	1.0446638565008213	5.254266914631285E-6	0.0	3.9801071878331983E-4	2.6271334573156425E-6
38	1.0590920734483988	5.254266914631285E-6	0.0	3.9801071878331983E-4	2.6271334573156425E-6
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTGCGC	8580	0.0	83.86189	44
TCGTGCG	14855	0.0	78.16883	43
CTATCAT	28045	0.0	72.00138	44
CTATACG	11515	0.0	67.70781	44
CCCTATC	23365	0.0	53.8833	42
CCTTAGC	46715	0.0	51.18056	43
AACGACA	9930	0.0	49.252087	43
CTCGTGC	19820	0.0	49.017147	42
ACCTTAG	36155	0.0	46.537785	42
CCTATCA	26545	0.0	45.690586	43
AACCTTA	51050	0.0	43.81683	41
TATGTTC	27950	0.0	43.3938	43
ACGACAC	5390	0.0	42.8457	44
TCGCAAA	6105	0.0	42.492477	43
GAACACA	41960	0.0	40.82344	44
CCCCTAT	27510	0.0	40.26015	41
ATGTTCT	26790	0.0	39.325745	44
ACTCGTG	28080	0.0	38.79406	41
TGCGGTC	7045	0.0	37.727406	42
GACAACC	59490	0.0	37.29387	38
>>END_MODULE