FastQCFastQC Report
Tue 24 May 2016
ERR841051.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR841051.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences93089
Sequences flagged as poor quality0
Sequence length24-50
%GC31

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAA31303.362373642428214No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAA30623.289325269365875No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAA27272.9294546079558272No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAA24182.5975142068343198No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA22672.435303849004716No Hit
AAAAAAAAAAAAAAAAAAAAAAAA17421.8713274393322519No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAA15951.713414044623962No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA9421.0119348150694496No Hit
CTAGGCAGGTGCTGGGGGCTTCCGAGACAATC7590.8153487522693336No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAA6540.7025534703348408No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAA6080.6531383944397298No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAA6080.6531383944397298No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5130.551085520308522No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAA4850.5210067784593239No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4600.4941507589511113No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAA4460.47911138802651226No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAA4400.47266594334454126No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4070.4372159975937006No Hit
CTAGGCTTGTGTGGTGTAGATGTTCGCGATTGTCTCGGAAGCCCCC3880.4168054227674591No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3750.4028402926231886No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAA3630.3899494032592465No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAA3340.35879642062971995No Hit
TAAAAAAAAAAAAAAAAAAAAAAAA3300.35449945750840595No Hit
CTAGGCAGGTGCTGGGGGCTTCCGAGACAATCGCG3150.33838584580347836No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2770.2975646961509953No Hit
CTAGGCTTGTGTGGTGTAGATGTTCGCGATTGTCTCGG2640.28359956600672476No Hit
TAAAAAAAAAAAAAAAAAAAAAAA2530.2717829174231112No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGC2400.2578177872788407No Hit
CAAAAAAAAAAAAAAAAAAAAAAAA2370.25459506493785516No Hit
ATCGGAAGAGCTCGTATGCCGTCTTCTGCTTG2330.25029810181654116Illumina Single End Adapter 1 (100% over 32bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAG2300.24707537947555566No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAG2290.24600113869522716No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAG2280.24492689791489863No Hit
ACATAAAAAGTAAAAATGTCTACAGTAGAGTCAATAGCAAGGCCACGACG2240.2406299347935846No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2120.22773904542964257No Hit
TTAGCACAAAGCCTAGAAAAGAGTGGAGGATGGAGTCAGAGAGGTGACAA2090.22451632308865707No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2060.22129360074767157No Hit
CTAGGCAGGTGCTGGGGGCTTCCGAGACAATCGC1860.19980878514110154No Hit
TCGGGCGAGGCCTATATTACGGATCATTTCTCTACTCAGAAACCTGAAAC1820.1955118220197875No Hit
CTAGGCTTGTGTGGTGTAGATGTTCGCGATTGTCTCGGAAG1770.190140618118145No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTA1750.187992136557488No Hit
CTTTTTGTCCGACATCTTGACGAGGCTGCGGTGTCTGCTGCTATTCTCCG1720.1847694142165025No Hit
CAAAAAAAAAAAAAAAAAAAAAAA1710.183695173436174No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAG1710.183695173436174No Hit
CAGACATGGTGGAACATGCCTGCAGTTCCATCTTCTTGGGATGCTGAGAT1690.18154669187551697No Hit
TGCTCGAGTAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAA1660.17832396953453147No Hit
TGCTCGAGTAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA1630.17510124719354597No Hit
ACATAAAAAGTAAAAATGTCTACAGTAGAGTCAATAGCAAGGCCACGA1630.17510124719354597No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAG1620.17402700641321744No Hit
ATACCCGCTGAACTTAAGCATATCAATAAGCGGAGGAAAAGAAACCAACA1610.17295276563288894No Hit
AGAATCGTTTGAACCTGGGAGGCGGAGGTTGCAGTGAGCCAAGATTGTGC1610.17295276563288894No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1510.1622103578296039No Hit
TGCTCGAGTAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA1510.1622103578296039No Hit
TGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAA1490.1600618762689469No Hit
TGCTCGAGTAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA1470.1579133947082899No Hit
TGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA1460.1568391539279614No Hit
ATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA1430.1536164315869759Illumina Single End Adapter 1 (100% over 32bp)
TGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1400.1503937092459904No Hit
AAAAAAAAAAAAAAAAAAAAAAAAG1370.14717098690500488No Hit
TGGATGAGTGTTTAGATTGATGGATGGATGAGTGGATGAACAAGTGGA1310.14072554222303385No Hit
TGCTCGAGTAGGGAAAAAAAAAAAAAAAAAAAAAAAAAA1310.14072554222303385No Hit
CTAGGCTTGTGTGGTGTAGATGTTCGCGATTGTCTCGGAA1300.13965130144270538No Hit
TGCTCGAGTAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA1290.13857706066237688No Hit
TGCTCGAGTAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1270.13642857910171985No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1260.13535433832139135No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1260.13535433832139135No Hit
TTAGCACAAAGCCTAGAAAAGAGTGGAGGATGGAGTCAGAGAGGTGAC1260.13535433832139135No Hit
TGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAA1260.13535433832139135No Hit
CTATCGCGAACATCTACACCACACAAGCAGGTGCTGGGGGCTTCCGAGAC1240.13320585676073435No Hit
TGCTCGAGTAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1230.13213161598040585No Hit
ACCTGCGTGCTGCCGAGAGAGGAAGCGAAGGGCACCATCTTTGTATTTTG1200.12890889363942035No Hit
ATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA1190.12783465285909182Illumina Single End Adapter 1 (100% over 32bp)
CTTTTTGTCCGACATCTTGACGAGGCTGCGGTGTCTGCTGCTATTCTC1190.12783465285909182No Hit
ATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAA1190.12783465285909182Illumina Single End Adapter 1 (100% over 32bp)
CTAGGCAGGTGCTGGGGGCTTCCGAGACAATCGCGAACATCTACAC1180.12676041207876332No Hit
AAAAAAAAAAAAAAAAAAAAAAAG1180.12676041207876332No Hit
CAGACATGGTGGAACATGCCTGCAGTTCCATCTTCTTGGGATGCTGAG1150.12353768973777783No Hit
ATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGGA1150.12353768973777783Illumina Single End Adapter 1 (100% over 32bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1140.12246344895744932No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1110.11924072661646382No Hit
TGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA1090.11709224505580682No Hit
AGTAGGGCTAGGCAGGTGCTGGGGGCTTCCGAGACAATC1090.11709224505580682No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1080.1160180042754783No Hit
TGCTCGAGTAGGGAAAAAAAAAAAAAAAAAAAAAAAAA1070.1149437634951498No Hit
AATTCGTGGAGAAAGAAATGGCTC1060.11386952271482129No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1060.11386952271482129No Hit
TGCTCGAGTAGGGGAAAAAAAAAAAAAAAAAAAAAAAA1050.1127952819344928No Hit
AGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTG1040.1117210411541643Illumina Single End Adapter 2 (100% over 34bp)
ACATAAAAAGTAAAAATGTCTACAGTAGAGTCAATAGCAAGGCCAC1040.1117210411541643No Hit
CTAGGCTTGTGTGGTGTAGATGTTCGCGATTGTCTCGGAAGCCCCCAG1020.10957255959350728No Hit
CTATCGCGAACATCTACACCACACAAGCAGGTGCTGGGGGCTTCCGAG990.10634983725252178No Hit
TCGGGCGAGGCCTATATTACGGATCATTTCTCTACTCAGAAACCTGAA970.10420135569186477No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA940.10097863335087925No Hit
ATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA940.10097863335087925Illumina Single End Adapter 1 (100% over 32bp)
TGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA940.10097863335087925No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGAGAT254.0017767E-11444.2857444
ATTGTGC254.0017767E-11444.2857444
CACGACG207.4305717E-9444.285744
CTGAAAC102.42706E-4444.285744
TTCTCCG151.3489716E-6444.285744
CATAGTT102.42706E-4444.285744
GTGACAA300.0444.285744
ACCAACA204.255977E-6333.214344
CTATCAT158.177827E-4296.1904644
CCTATCA100.0013144298253.6051643
CCACGAC201.2600867E-7253.6051643
TATAGAC100.0013144298253.6051643
TAAGGTG100.0013144298253.6051643
GATTGTG251.2314558E-9253.6051343
ATTCTCC151.2905737E-5253.6051343
CCCAGCA200.0019352528222.1428544
CTTAACA200.0019352528222.1428544
GGTGACA408.913048E-11190.2038743
GCTGAGA408.913048E-11190.2038743
ATATTGG204.0662286E-5190.2038743
Produced by FastQC (version 0.11.5)