FastQCFastQC Report
Tue 24 May 2016
ERR841050.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR841050.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3995449
Sequences flagged as poor quality0
Sequence length24-50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGC1159772.9027275783022133No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTA769321.9254907270747295No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCT428221.0717694056412683No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATCAT266160.6661579211748166No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATC162090.40568656989489793No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCTA141410.3539276812193073No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACC115030.2879025611389358No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTA94780.23721989693774093No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTC92170.23068746466292026No Hit
TCAGTTCTTTGACCATTCGTTGGAGCTCCGGTTTTCAGCCTCTTTCCG81880.2049331627058686No Hit
ATAGGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACATGC80840.20233020118639983No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA79110.1980002748126681No Hit
TCAGTTCTTTGACCATTCGTTGGAGCTCCGGTTTTCAGCCTCTTTCCGG74290.18593654930897627No Hit
CTGATGTTCGCCGACCGTTGACTATTCTCTACAAACCACAAAGACATTGG60260.15082159727229655No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTT57890.1448898484250456No Hit
CCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCTAG54460.13630508110602838No Hit
TCAGTTCTTTGACCATTCGTTGGAGCTCCGGTTTTCAGCCTCTTTC52190.13062361702026482No Hit
AAGATAAAATTTGAAATCTGGTTAGGCTGGTGTTAGGGTTCTTTGTTTTT52100.13039836073492617No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACT51350.12852122502377078No Hit
CCTTTCCGGCGGTGACGACCTACGCACACGAGAACATGCCTCTCGCAAA50700.1268943740741028No Hit
TCAGTTCTTTGACCATTCGTTGGAGCTCCGGTTTTCAGCCTCTTTCC48020.12018674246624098No Hit
TCCCTGGTGTGATTCCGTCCTGCGCGGTTGTTCTCTGGAGCAGCGTTCT46160.11553144590257566No Hit
CTTTTTCGCAACGGGTTTGCCGCCAGAACACAGGTGTCGTGAAAACTAC45810.11465544923736981No Hit
CTCTCGGCCTTAGCGCCATTTTTTTGGAAACCTCTGCGCCATGAGAGCC44750.11200243076560357No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA42320.10592051106146018No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATCAT43600.0169.2864544
ATTAGCG150.004477453169.0925344
CGCCTAC309.908654E-7169.0925344
AATCGCA6450.0157.2953844
ACTTCTA33450.0125.8715944
ACATTGG13750.0118.05733544
GGTTACG652.8830982E-9117.06406444
GACCGAT551.4571378E-7115.2903744
CTAGATA457.450668E-6112.7283644
TGCGAAT901.8189894E-12112.7283644
ACGAACA5000.0109.0646944
ATTATGC2950.090.2782244
CCTTAGC168600.086.31949643
AACTACC7750.085.0917344
GTATAGA603.1089538E-584.54626544
CATGACC8300.080.98106444
CCTATCA43900.078.4640743
TATGCGC500.00159754776.09164444
GACATTG9950.074.97816543
ACTACCC2200.074.9387444
Produced by FastQC (version 0.11.5)