##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR841050.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3995449 Sequences flagged as poor quality 0 Sequence length 24-50 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 37.273683383269315 39.0 37.0 39.0 34.0 39.0 2 37.81048563002556 39.0 38.0 39.0 35.0 39.0 3 38.07378620024933 39.0 38.0 39.0 35.0 39.0 4 38.76058085086307 39.0 38.0 41.0 35.0 41.0 5 39.32378939138004 40.0 39.0 41.0 35.0 41.0 6 39.27019391312466 40.0 39.0 41.0 35.0 41.0 7 39.28305129160702 40.0 39.0 41.0 35.0 41.0 8 39.266176842702784 40.0 39.0 41.0 35.0 41.0 9 39.27552547911386 40.0 39.0 41.0 35.0 41.0 10 39.24070411110241 40.0 39.0 41.0 35.0 41.0 11 39.145728052091265 40.0 39.0 41.0 35.0 41.0 12 39.12746652503886 40.0 38.0 41.0 35.0 41.0 13 38.98903452402971 40.0 38.0 41.0 35.0 41.0 14 38.93898132600366 40.0 38.0 41.0 35.0 41.0 15 38.90468155143515 40.0 38.0 41.0 35.0 41.0 16 38.84577778367338 40.0 38.0 41.0 35.0 41.0 17 38.83515895209775 40.0 38.0 41.0 35.0 41.0 18 38.77572908576733 40.0 38.0 41.0 35.0 41.0 19 38.74258012053213 40.0 38.0 41.0 35.0 41.0 20 38.655010738467695 40.0 38.0 41.0 35.0 41.0 21 38.580416619008275 40.0 38.0 41.0 35.0 41.0 22 38.495969038773865 40.0 38.0 41.0 35.0 41.0 23 38.37246001638364 40.0 37.0 41.0 34.0 41.0 24 38.216996137355274 40.0 37.0 41.0 34.0 41.0 25 38.12388514387955 40.0 37.0 41.0 34.0 41.0 26 37.9758940014035 39.0 36.0 41.0 33.0 41.0 27 37.88544503481297 39.0 36.0 41.0 33.0 41.0 28 37.74622512213179 39.0 36.0 41.0 33.0 41.0 29 37.716629693449036 39.0 36.0 41.0 33.0 41.0 30 37.607834751287406 39.0 36.0 41.0 33.0 41.0 31 37.572402203830805 39.0 36.0 41.0 33.0 41.0 32 37.4625985916439 39.0 35.0 41.0 33.0 41.0 33 37.33441157723608 38.0 35.0 40.0 32.0 41.0 34 37.29698501433401 38.0 35.0 40.0 32.0 41.0 35 37.12356120121404 38.0 35.0 40.0 32.0 41.0 36 37.02946039535069 38.0 35.0 40.0 32.0 41.0 37 37.02865659462155 38.0 35.0 40.0 32.0 41.0 38 36.748019555067046 38.0 35.0 40.0 31.0 41.0 39 36.716286298357154 38.0 35.0 40.0 31.0 41.0 40 36.471226562192754 37.0 34.0 40.0 31.0 41.0 41 36.41190794396847 37.0 34.0 40.0 31.0 41.0 42 36.27330058960143 37.0 34.0 40.0 31.0 41.0 43 36.06693812590373 37.0 34.0 40.0 30.0 41.0 44 35.84802017175802 37.0 34.0 40.0 30.0 41.0 45 35.581616519333835 36.0 33.0 40.0 29.0 41.0 46 35.203027622370655 36.0 33.0 40.0 28.0 41.0 47 35.29362085539224 36.0 33.0 39.0 29.0 41.0 48 36.26627108047791 37.0 34.0 40.0 31.0 41.0 49 36.74967664945564 38.0 35.0 40.0 32.0 41.0 50 36.66921521107542 38.0 35.0 40.0 32.0 41.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 19 2.0 20 17.0 21 37.0 22 97.0 23 231.0 24 479.0 25 795.0 26 1400.0 27 2767.0 28 5524.0 29 11554.0 30 23192.0 31 44990.0 32 82902.0 33 144816.0 34 225892.0 35 239043.0 36 403727.0 37 669035.0 38 697561.0 39 628163.0 40 813225.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 0.0 39.18630922331883 20.551983018679504 40.26170775800167 2 20.875175731188158 17.800352350887223 38.930943681173254 22.393528236751365 3 20.317015684595148 25.959810774708924 28.78812869342094 24.935044847274987 4 18.896824862487293 26.47424607347009 32.668743863330505 21.96018520071211 5 16.04785845095257 26.736169076366632 31.891259280246096 25.324713192434693 6 18.909113844276327 23.87909343855972 27.948097948440836 29.263694768723113 7 21.720887940254023 22.6140541400979 23.65889290540312 32.00616501424496 8 22.671769806096886 22.165018249513384 32.43415195638838 22.72905998800135 9 23.898815877764925 29.5962481313114 23.750221814869867 22.754714176053806 10 30.12064476357976 20.125472756628852 26.033419523062364 23.72046295672902 11 25.099081479953817 20.681805724463008 24.562195638087232 29.656917157495943 12 23.150038956823128 23.9677192726024 23.787814586045272 29.0944271845292 13 24.2087685263909 21.90937739413017 24.05229049350899 29.829563585969936 14 20.90390842180691 23.63949583638785 22.484606861456623 32.97198888034862 15 21.539206231890333 34.08500521468301 22.983149077863338 21.39263947556332 16 20.346198887784578 33.41038766856991 22.25044043860903 23.99297300503648 17 21.44922886013562 34.441836199135565 21.209606229487598 22.899328711241214 18 20.59966226574285 25.258187502831348 20.681255097987737 33.460895133438065 19 22.588725322235373 23.58691100799935 22.056720033217793 31.767643636547483 20 22.07381448242738 22.46583550434507 25.354947591622366 30.10540242160518 21 23.376496609016908 32.120695321101586 22.106526700753783 22.396281369127724 22 24.744828428544576 23.890080939589016 21.33692608765623 30.028164544210174 23 25.52551665657602 23.565236347654544 29.348966786961867 21.56028020880757 24 24.72951100114155 25.861323721063638 22.246586053282122 27.16257922451269 25 24.62220598951632 24.187092332486397 22.426640285165714 28.764061392831568 26 24.426644553268716 33.59117400184874 22.0662602537138 19.91592119116874 27 24.536624623695214 26.023798446218834 21.575461008359387 27.86411592172657 28 25.08168164013967 23.94541406968493 21.52099232241256 29.451911967762847 29 23.47573181175645 26.743347400352878 28.052560438313197 21.728360349577475 30 25.79315710245546 26.820274678775817 27.957546291571656 19.429021927197063 31 25.594577584272045 31.69607706187981 21.909580364957815 20.799764988890338 32 21.570506969737437 26.231205257904914 24.12438714014363 28.073900632214016 33 22.130896422537923 23.798898626240344 33.652058517476966 20.418146433744763 34 23.31417595381948 31.992370137971026 24.29937068048325 20.394083227726245 35 21.109355421366434 24.122246134692674 24.62963569676505 30.138762747175846 36 22.009800676346646 24.324403197717228 24.93819521099016 28.727600914945967 37 21.15208252064809 35.85563667618165 20.813688535188955 22.178592267981305 38 29.276332501694867 25.49503611809303 21.100636266247406 24.127995113964698 39 21.833975671857875 34.17064113915057 22.51775627822801 21.477626910763554 40 19.34674633300893 26.72797658974954 24.877905440623298 29.04737163661823 41 20.633078796190357 33.203162235212 22.953767642633093 23.20999132596455 42 20.116335912123056 34.05116006069112 22.2021559892443 23.63034803794153 43 20.43705013280876 24.726147940907666 23.350793798490326 31.48600812779325 44 20.847390823154207 25.80722254663205 21.79583838141553 31.54954824879821 45 21.299153306886165 24.648037099495134 32.377693642307015 21.675115951311682 46 20.72686144566312 26.399529135937183 28.89029936846476 23.98331004993494 47 20.398857870138713 32.825240532499926 25.18817334544461 21.58772825191675 48 27.48011012673454 24.431078123798102 22.800704786320992 25.28810696314637 49 19.096737359899823 25.813894288283013 21.249759050504487 33.83960930131268 50 19.395721743572995 28.65051097533964 27.251209736743824 24.70255754434354 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 77012.0 1 65894.0 2 54260.5 3 51148.0 4 43906.5 5 29256.0 6 14539.0 7 10222.5 8 10365.0 9 9755.0 10 8841.5 11 7779.0 12 7000.5 13 6406.5 14 6145.5 15 6358.5 16 6521.5 17 6924.0 18 8107.5 19 9598.0 20 10760.0 21 12052.5 22 13588.5 23 15906.0 24 18170.0 25 20172.0 26 22811.5 27 25535.5 28 28935.0 29 35150.0 30 45144.5 31 68715.0 32 104035.5 33 114788.0 34 112073.0 35 120911.5 36 124657.5 37 127020.0 38 130436.0 39 126921.0 40 126053.0 41 139674.5 42 158766.0 43 175274.5 44 189153.5 45 199767.0 46 222803.5 47 246608.5 48 304851.5 49 376488.0 50 453667.0 51 519876.0 52 462633.0 53 371589.0 54 335175.5 55 319013.5 56 312210.0 57 307273.0 58 295744.5 59 268190.5 60 232845.0 61 201617.5 62 172967.0 63 142996.0 64 117457.0 65 101781.0 66 79701.5 67 56716.5 68 41826.5 69 31360.0 70 24486.5 71 17450.0 72 12084.0 73 8057.5 74 5091.5 75 3552.0 76 2879.0 77 2351.5 78 1498.5 79 830.0 80 489.5 81 399.5 82 347.5 83 293.0 84 248.5 85 177.5 86 116.0 87 53.5 88 4.0 89 3.0 90 1.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 >>END_MODULE >>Sequence Length Distribution warn #Length Count 24 19936.0 25 22555.0 26 23962.0 27 25426.0 28 26890.0 29 25709.0 30 28173.0 31 26457.0 32 26322.0 33 22638.0 34 23615.0 35 23001.0 36 19553.0 37 20166.0 38 23037.0 39 27283.0 40 33383.0 41 30201.0 42 43608.0 43 68236.0 44 186305.0 45 387870.0 46 405010.0 47 519072.0 48 577835.0 49 740683.0 50 618523.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 10.610507200051188 #Duplication Level Percentage of deduplicated Percentage of total 1 71.5756495581784 7.594539449853925 2 12.045113140498511 2.556095194053813 3 4.326760428203003 1.3772736802903358 4 2.0652136928990674 0.8765185903259944 5 1.187117938321881 0.629796171593712 6 0.7602004671463661 0.48396675180832766 7 0.5648589613302126 0.4195408053345361 8 0.41097122872797837 0.3488490545145679 9 0.35613059184373297 0.3400853588024778 >10 3.3390174297370545 8.21098214736846 >50 1.4151318122745649 11.045143089733058 >100 1.7912635150044514 33.09884814082419 >500 0.096019670859679 6.890297268723228 >1k 0.059929518708972056 12.342340540647118 >5k 0.004304330073020093 3.0986804520309494 >10k+ 0.0023177161931646657 10.687043304095315 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGC 115977 2.9027275783022133 No Hit CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTA 76932 1.9254907270747295 No Hit CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCT 42822 1.0717694056412683 No Hit ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATCAT 26616 0.6661579211748166 No Hit ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATC 16209 0.40568656989489793 No Hit TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCTA 14141 0.3539276812193073 No Hit CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACC 11503 0.2879025611389358 No Hit ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTA 9478 0.23721989693774093 No Hit TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTC 9217 0.23068746466292026 No Hit TCAGTTCTTTGACCATTCGTTGGAGCTCCGGTTTTCAGCCTCTTTCCG 8188 0.2049331627058686 No Hit ATAGGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACATGC 8084 0.20233020118639983 No Hit TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 7911 0.1980002748126681 No Hit TCAGTTCTTTGACCATTCGTTGGAGCTCCGGTTTTCAGCCTCTTTCCGG 7429 0.18593654930897627 No Hit CTGATGTTCGCCGACCGTTGACTATTCTCTACAAACCACAAAGACATTGG 6026 0.15082159727229655 No Hit CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTT 5789 0.1448898484250456 No Hit CCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCTAG 5446 0.13630508110602838 No Hit TCAGTTCTTTGACCATTCGTTGGAGCTCCGGTTTTCAGCCTCTTTC 5219 0.13062361702026482 No Hit AAGATAAAATTTGAAATCTGGTTAGGCTGGTGTTAGGGTTCTTTGTTTTT 5210 0.13039836073492617 No Hit TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACT 5135 0.12852122502377078 No Hit CCTTTCCGGCGGTGACGACCTACGCACACGAGAACATGCCTCTCGCAAA 5070 0.1268943740741028 No Hit TCAGTTCTTTGACCATTCGTTGGAGCTCCGGTTTTCAGCCTCTTTCC 4802 0.12018674246624098 No Hit TCCCTGGTGTGATTCCGTCCTGCGCGGTTGTTCTCTGGAGCAGCGTTCT 4616 0.11553144590257566 No Hit CTTTTTCGCAACGGGTTTGCCGCCAGAACACAGGTGTCGTGAAAACTAC 4581 0.11465544923736981 No Hit CTCTCGGCCTTAGCGCCATTTTTTTGGAAACCTCTGCGCCATGAGAGCC 4475 0.11200243076560357 No Hit TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 4232 0.10592051106146018 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.05088289201038482 0.0 0.0 0.0 0.0 2 0.09358147232013223 0.0 0.0 0.0 0.0 3 0.1236156436986181 0.0 0.0 0.0 0.0 4 0.14498996232964054 0.0 0.0 0.0 0.0 5 0.16326074991821946 0.0 0.0 0.0 0.0 6 0.19349514910589524 0.0 0.0 0.0 0.0 7 0.21912430868220317 0.0 0.0 0.0 0.0 8 0.24955893567906887 0.0 0.0 0.0 0.0 9 0.2740117568763861 0.0 0.0 0.0 0.0 10 0.29746343902775385 0.0 0.0 0.0 0.0 11 0.3185374159449914 0.0 0.0 0.0 0.0 12 0.347069878754553 0.0 0.0 0.0 0.0 13 0.369920877478351 0.0 0.0 0.0 0.0 14 0.3919959934415381 0.0 0.0 0.0 0.0 15 0.4185512066353494 0.0 0.0 0.0 2.5028476148738227E-5 16 0.4435796827840876 0.0 0.0 0.0 2.5028476148738227E-5 17 0.4746900786369692 0.0 0.0 0.0 2.5028476148738227E-5 18 0.5085035499139146 0.0 0.0 0.0 2.5028476148738227E-5 19 0.5324307731121083 0.0 0.0 0.0 2.5028476148738227E-5 20 0.5569586797378717 0.0 0.0 0.0 2.5028476148738227E-5 21 0.5799348208424134 0.0 0.0 0.0 2.5028476148738227E-5 22 0.598330750811736 0.0 0.0 0.0 2.5028476148738227E-5 23 0.62185751839155 0.0 0.0 0.0 2.5028476148738227E-5 24 0.6427312674995976 0.0 0.0 0.0 2.5028476148738227E-5 25 0.6625287921332496 0.0 0.0 0.0 2.5028476148738227E-5 26 0.6877074391388802 0.0 0.0 0.0 2.5028476148738227E-5 27 0.7119350040508589 0.0 0.0 0.0 2.5028476148738227E-5 28 0.7376392490556131 0.0 0.0 0.0 2.5028476148738227E-5 29 0.7645198324393578 0.0 0.0 0.0 2.5028476148738227E-5 30 0.7889225966843777 0.0 0.0 0.0 2.5028476148738227E-5 31 0.8116234245512832 0.0 0.0 0.0 2.5028476148738227E-5 32 0.836601843747724 0.0 0.0 0.0 2.5028476148738227E-5 33 0.86107969342119 0.0 0.0 0.0 2.5028476148738227E-5 34 0.883455151098162 0.0 0.0 0.0 2.5028476148738227E-5 35 0.9040285584924248 0.0 0.0 0.0 2.5028476148738227E-5 36 0.9232754566508045 0.0 0.0 0.0 2.5028476148738227E-5 37 0.9414461303347884 0.0 0.0 0.0 2.5028476148738227E-5 38 0.9536600266953726 0.0 0.0 0.0 2.5028476148738227E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTATCAT 4360 0.0 169.28645 44 ATTAGCG 15 0.004477453 169.09253 44 CGCCTAC 30 9.908654E-7 169.09253 44 AATCGCA 645 0.0 157.29538 44 ACTTCTA 3345 0.0 125.87159 44 ACATTGG 1375 0.0 118.057335 44 GGTTACG 65 2.8830982E-9 117.064064 44 GACCGAT 55 1.4571378E-7 115.29037 44 CTAGATA 45 7.450668E-6 112.72836 44 TGCGAAT 90 1.8189894E-12 112.72836 44 ACGAACA 500 0.0 109.06469 44 ATTATGC 295 0.0 90.27822 44 CCTTAGC 16860 0.0 86.319496 43 AACTACC 775 0.0 85.09173 44 GTATAGA 60 3.1089538E-5 84.546265 44 CATGACC 830 0.0 80.981064 44 CCTATCA 4390 0.0 78.46407 43 TATGCGC 50 0.001597547 76.091644 44 GACATTG 995 0.0 74.978165 43 ACTACCC 220 0.0 74.93874 44 >>END_MODULE