FastQCFastQC Report
Tue 24 May 2016
ERR841049.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR841049.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2118323
Sequences flagged as poor quality0
Sequence length24-50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTGCAG1111375.246461469756973No Hit
AAGCAGTGGTATCAACGCAGAGTGCAGTG685293.2350590537892474No Hit
AAGCAGTGGTATCAACGCAGAGTG622402.9381732625288968No Hit
AAGCAGTGGTATCAACGCAGAGTGCAGT612712.892429530340746No Hit
AAGCAGTGGTATCAACGCAGAGTGCAGA466762.2034411182808284No Hit
AAGCAGTGGTATCAACGCAGAGTGCAGTGCTTT452952.1382480386607705No Hit
AAGCAGTGGTATCAACGCAGAGTGCAGTGC452342.1353684022691533No Hit
AAGCAGTGGTATCAACGCAGAGTGCAGTGCT448012.1149277046040664No Hit
AAGCAGTGGTATCAACGCAGAGTGCA433972.048648860442907No Hit
AAGCAGTGGTATCAACGCAGAGTGCAGTGCTTTCC294811.391714105922468No Hit
AAGCAGTGGTATCAACGCAGAGTGCAGTGCTT226081.0672593367489283No Hit
AAGCAGTGGTATCAACGCAGAGTGCAGTGCTTTCCG225341.0637660073558188No Hit
AAGCAGTGGTATCAACGCAGAGTGC197380.9317748048810309No Hit
AAGCAGTGGTATCAACGCAGAGTGCAGG182600.8620026313267618No Hit
AAGCAGTGGTATCAACGCAGAGTGCAGTGCTTTCCGCGGGAAGCA178940.8447248129770577No Hit
AAGCAGTGGTATCAACGCAGAGTGCAGTGCTTTCCGCGGG163970.7740557034975308No Hit
AAGCAGTGGTATCAACGCAGAGTGCAGTGCTTTC158140.7465339327383029No Hit
AAGCAGTGGTATCAACGCAGAGTGCAGTGCTTTCCGCGGGAAGCAG157080.7415299744184433No Hit
AAGCAGTGGTATCAACGCAGAGTGCAGTGCTTTCCGC114870.5422685775493161No Hit
AAGCAGTGGTATCAACGCAGAGTGCAGATCGGAAGAGCTCGTATGCCGTC106110.5009151106795329Illumina Single End Adapter 2 (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTGCAGTGCTTTCCGCG93170.4398290534540767No Hit
AAGCAGTGGTATCAACGCAGAGTGCAGATCGG88420.41740565532263024No Hit
AAGCAGTGGTATCAACGCAGATCGGAAGAGCTCGTATGCCGTCTTCTGCT87660.4138179116215988Illumina Single End Adapter 2 (100% over 32bp)
AAGCAGTGGTATCAACGCAGAGTGCAGTGCTTTCCGCGGGAAGCAGTGGT82660.3902143346411288No Hit
AAGCAGTGGTATCAACAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTG80630.38063128238705807Illumina Single End Adapter 2 (100% over 34bp)
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGC75690.3573109483303538No Hit
AAGCAGTGGTATCAACGCAGAGTGCAGTGCTTTCCGCGG74640.3523541971644551No Hit
AAGCAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAA68270.3222832400913364Illumina Single End Adapter 2 (100% over 34bp)
AAGCAGTGGTATCAACGCAGATCGGAAGAGCTCGTATGCCGTCTTCTG63750.3009456065009916Illumina Single End Adapter 2 (100% over 30bp)
AAGCAGTGGTATCAACGCAGAGTGCAGATCGGAAGAGCTCGTATGCCG56910.26865591319170873Illumina Single End Adapter 2 (100% over 23bp)
AAGCAGTGGTATCAACGCAGAGTGCAGTGCTTTCCGCGGGAAGCACTG53350.2518501663816141No Hit
AAGCAGTGGTATCAACGCAGAGTGCAGGATCGGAAGAGCTCGTATGCCGT52790.2492065657598015Illumina Single End Adapter 2 (95% over 24bp)
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATCAT52070.24580765067461385No Hit
AAGCAGTGGTATCAACGCAGAGTGCAGTGCTTTCCGCGGGAAGCAGTGG51120.24132297104832454No Hit
AAGCAGTGGTATCAACGCAGAGTGCAGATCGGAAGAGCTCGTATGC49820.23518604103340235Illumina Single End Adapter 2 (100% over 21bp)
AAGCAGTGGTATCAACAGATCGGAAGAGCTCGTATGCCGTCTTCTGCT48880.23074856856107404Illumina Single End Adapter 2 (100% over 32bp)
AAGCAGTGGTATCAACGCAGAGTGCAGTGCTTTCCGCGGGAAGCACTGCA48880.23074856856107404No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTA46720.22055182330551099No Hit
AAGCAGTGGTATCAACGCAGAGTGCAGATCG45330.2139900289049404No Hit
AAGCAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTG43830.2069089558107994Illumina Single End Adapter 2 (100% over 34bp)
AAGCAGTGGTATCAACGCAGAGTGCAGTGCTTTCCGCGGGAAGCAC42040.19845887525179115No Hit
AAGCAGTGGTATCAACGCAGATCGGAAGAGCTCGTATGCCGTCTTC35780.16890719687224282Illumina Single End Adapter 2 (100% over 28bp)
AAGCAGTGGTATCAACGCAGAGATCGGAAGAGCTCGTATGCCGTCTTCTG35650.1682935038707506Illumina Single End Adapter 2 (100% over 30bp)
AAGCAGTGGTATCAACAGATCGGAAGAGCTCGTATGCCGTCTTCTG34250.161684502316219Illumina Single End Adapter 2 (100% over 30bp)
AAGCAGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAA33840.15974900900382047Illumina Single End Adapter 2 (97% over 34bp)
AAGCAGTGGTATCAACGCAGAGTGCAGTGCTTTCCGCGGGAAGCAGTG32730.15450901491415617No Hit
AAGCAGTGGTATCAACGCAGAGTGCAGTA31060.14662542020267919No Hit
AAGCAGTGGTATCAACGCAGAGTGCAGTGCTTTCCGCGGAAAGCACTGCA29480.1391666898768507No Hit
AAGCAGTGGTATCAACGCAGAGTGCAGTGCTTTCCGCGGGAAG29470.13911948272288974No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCT29220.13793930387386627No Hit
AAGCAGTGGTATCAACGCAGAGTGCAGGATCGGAAGAGCTCGTATG28540.13472921740452234No Hit
AAGCAGTGGTATCAACGCAGATCGGAAG28520.13463480309660047No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATC28490.13449318163471766No Hit
AAGCAGTGGTATCAACGCAGATCGGAAGAGCT27830.13137750947329563No Hit
AAGCAGTGGTATCAACGCAGAGTGCAGGATCGGAAGAGCTCGTATGCC25850.12203049298902954Illumina Single End Adapter 2 (95% over 22bp)
AAGCAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAA25500.12037824260039663Illumina Single End Adapter 2 (100% over 34bp)
AAGCAGTGGTATCAACGCAGGATCGGAAGAGCTCGTATGCCGTCTTCTGC25410.11995337821474818Illumina Single End Adapter 2 (96% over 31bp)
AAGCAGTGGTATCAACGCAGAGTGCG24630.11627122020579488No Hit
AAGCAGTGGTATCAACGCAGAGTGCAGAG24280.11461896981716196No Hit
AAGCAGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTG23270.10985104726710705Illumina Single End Adapter 2 (97% over 34bp)
AAGCAGTGGTATCAACGCAGAGTGCAGTAGAT22890.10805717541659134No Hit
AAGCAGTGGTATCAACGCAGAGTGCAGATC22210.10484708894724744No Hit
AAGCAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA21950.103619702944263Illumina Single End Adapter 2 (100% over 34bp)
AAGCAGTGGTATCAACGCAGAGTGCAGTGCTTTCCGCGGGAA21860.10319483855861453No Hit
AAGCAGTGGTATCAACGCAGAGATCGGAAGAGCTCGTATGCCGTCTTC21350.10078727370660659Illumina Single End Adapter 2 (100% over 28bp)
AAGCAGTGGTATCAACGCAGAGAG21210.10012637355115343No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATAGT100.0017327104232.2046544
GAGACGT100.004024943175.2158543
CTACGGG208.296065E-7175.2158543
ACGTCTA208.296065E-7175.2158543
GTCCCGT301.7462298E-10175.2158443
CCGCATA301.7462298E-10175.2158443
CGAGGAT550.0168.8761144
CTAATTC450.0155.7474243
GGGGTAA301.5366604E-6154.803144
TCGGGGC303.5563062E-8146.013243
TGGCCGA500.0140.1726743
GGCGTGC201.8192979E-4131.4118843
CCATAAG201.8192979E-4131.4118843
CTATCAT8600.0129.6025844
CAATGTC457.444396E-8129.002644
AACGACA1050.0125.1541843
GGGCCGA306.240267E-6116.8105643
GGCTAGT406.4292326E-6116.10232544
GGGGCCG206.4878204E-6115.9097942
GGGTAGG559.276846E-11111.5009943
Produced by FastQC (version 0.11.5)