FastQCFastQC Report
Tue 24 May 2016
ERR841048.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR841048.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3396314
Sequences flagged as poor quality0
Sequence length24-50
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAA339310.9990536799600979No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAA331940.9773536840233265No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAA305620.8998579047755891No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAA273310.8047253581382641No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA249230.7338249643584192No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGC195640.576036255776115No Hit
AAAAAAAAAAAAAAAAAAAAAAAA194090.5714724845818143No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAA176180.5187388445237985No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTA129090.3800885312724324No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA100750.29664512762954187No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCT71650.21096400391718786No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA61000.17960647925957376No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAA56190.1654440667146795No Hit
AGTGCAGTGCTTATTGTGGGAAAAAAAAAAAAAAAAAAAAAAAAA55890.1645607561609439No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACACA55700.16400132614357799No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAA54610.1607919644650053No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA50800.14957392043256307No Hit
AGTGCAGTGCTTATTGTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA49500.14574624136637543No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAA46990.13835587640012084No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAA45030.13258491411571485No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATCAT44840.13202548409834897No Hit
AGTGCAGTGCTTATTGTGGGAAAAAAAAAAAAAAAAAAAAAAAAAA39870.11739197259146238No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACA38260.11265153928641462No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAA36860.10852942336898178No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGTAA405.456968E-12162.7547344
CGTGCGT302.491106E-8155.0045244
GCGATAT651.8189894E-12114.4648744
TCGACGC453.3287506E-1094.9022643
CTATCAT12050.083.35512544
GTCCGCA603.6379788E-1181.34479543
TACGATT303.9119186E-880.4332342
TTCGACG550.080.4332342
TCGTGCG3250.075.08749443
CCCCCGT505.8595462E-574.4021644
GTACGGG301.1979137E-768.438341
CGTTAAC208.86735E-568.4382941
TCGTCTA303.676516E-667.0276942
ACCTTAG29450.063.09023742
ACGCCCC450.003598308462.001844
TATCGAC902.3704888E-762.001844
TCGCAAA9350.061.3348443
CGCGATA701.1008524E-861.00859543
TTGCGAC353.4794357E-758.66139641
CGCAAAT1100.058.4968942
Produced by FastQC (version 0.11.5)