##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR841048.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3396314
Sequences flagged as poor quality	0
Sequence length	24-50
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	37.015740594067566	39.0	37.0	39.0	33.0	39.0
2	37.684363695465144	39.0	37.0	39.0	35.0	39.0
3	37.76485095312153	39.0	37.0	39.0	35.0	39.0
4	38.20742634514948	39.0	38.0	39.0	35.0	41.0
5	39.06624181391944	40.0	38.0	41.0	35.0	41.0
6	38.96430041509707	40.0	38.0	41.0	35.0	41.0
7	39.07121897445289	40.0	38.0	41.0	35.0	41.0
8	38.97145699720344	40.0	38.0	41.0	35.0	41.0
9	39.06309575616389	40.0	38.0	41.0	35.0	41.0
10	39.04540893450959	40.0	38.0	41.0	35.0	41.0
11	39.008152073100426	40.0	38.0	41.0	35.0	41.0
12	38.96731721507493	40.0	38.0	41.0	35.0	41.0
13	38.824291570214065	40.0	38.0	41.0	35.0	41.0
14	38.775961233266415	40.0	38.0	41.0	35.0	41.0
15	38.60378810675338	40.0	38.0	41.0	35.0	41.0
16	38.44003587418596	40.0	37.0	41.0	35.0	41.0
17	38.29089830916694	40.0	37.0	41.0	35.0	41.0
18	38.29972405378301	40.0	37.0	41.0	35.0	41.0
19	38.28323617898698	40.0	37.0	41.0	35.0	41.0
20	38.2511169461952	40.0	36.0	41.0	35.0	41.0
21	38.106453643567704	40.0	36.0	41.0	34.0	41.0
22	38.04878406413541	40.0	36.0	41.0	34.0	41.0
23	37.960292540677926	40.0	36.0	41.0	34.0	41.0
24	37.757091658780666	39.0	35.0	41.0	33.0	41.0
25	37.68773174193694	39.0	35.0	41.0	33.0	41.0
26	37.5889931634687	39.0	35.0	41.0	33.0	41.0
27	37.498561680667	39.0	35.0	41.0	33.0	41.0
28	37.40785836007608	39.0	35.0	41.0	33.0	41.0
29	37.40605738063685	39.0	35.0	41.0	33.0	41.0
30	37.41842944413027	39.0	35.0	40.0	33.0	41.0
31	37.3903639084427	39.0	35.0	40.0	33.0	41.0
32	37.33395668484516	38.0	35.0	40.0	33.0	41.0
33	37.24431712909644	38.0	35.0	40.0	33.0	41.0
34	37.14030091454287	38.0	35.0	40.0	33.0	41.0
35	37.0097420755637	38.0	35.0	40.0	32.0	41.0
36	36.91694840915747	38.0	35.0	40.0	32.0	41.0
37	36.837357527533975	38.0	35.0	40.0	32.0	41.0
38	36.715161070099704	38.0	35.0	40.0	32.0	41.0
39	36.578306421714295	38.0	35.0	40.0	31.0	41.0
40	36.44168131924561	37.0	34.0	40.0	31.0	41.0
41	36.316293949173875	37.0	34.0	40.0	31.0	41.0
42	36.15452862961752	37.0	34.0	40.0	31.0	41.0
43	35.996666311484745	37.0	34.0	40.0	30.0	41.0
44	35.82759858257257	36.0	34.0	40.0	30.0	41.0
45	35.5510230002029	36.0	33.0	40.0	29.0	41.0
46	35.227746542096554	36.0	33.0	40.0	28.0	41.0
47	35.094313544166624	36.0	33.0	39.0	28.0	41.0
48	35.9068499288064	37.0	34.0	40.0	30.0	41.0
49	36.73103641529599	38.0	35.0	40.0	32.0	41.0
50	36.431153051035764	38.0	35.0	40.0	31.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	6.0
20	28.0
21	71.0
22	150.0
23	300.0
24	599.0
25	1142.0
26	1975.0
27	3544.0
28	6836.0
29	13110.0
30	24842.0
31	46044.0
32	79566.0
33	136658.0
34	196410.0
35	217134.0
36	403320.0
37	637676.0
38	597278.0
39	481771.0
40	547854.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	52.71088008941458	17.11923573615396	30.169884174431456
2	24.506008572823358	30.835841444577856	28.298031336325202	16.360118646273577
3	20.456235789741466	28.890909379992546	27.719904578905247	22.93295025136074
4	20.137066242991665	31.52261540010729	29.057943405703952	19.28237495119709
5	18.449236436913665	31.75180504511656	26.839185069460598	22.95977344850918
6	17.293777901572117	37.824594545733994	25.45250527483619	19.4291222778577
7	26.99794541965201	33.31644247263357	20.423494411883	19.26211769583142
8	21.13196836334921	31.691445490611294	27.02803686584927	20.14854928019023
9	24.046304316974226	34.2980066036297	24.17497322096838	17.480715858427693
10	21.60957437975405	30.100573739648333	24.145912303750478	24.143939576847135
11	21.239643919849577	32.65166294989215	26.83897896366473	19.26971416659355
12	21.86061123912571	31.04886650645376	27.712131446032373	19.37839080838815
13	19.749646234123226	33.16993069545396	25.34639023364742	21.7340328367754
14	19.835533463631457	31.1722355471255	28.086095690798906	20.906135298444138
15	18.10468643358653	34.95686794566109	28.11789487073339	18.82055075001899
16	22.90577372999081	38.1956438656732	20.090604107865172	18.80797829647082
17	20.7059182395974	36.31698953630318	23.731610210363353	19.24548201373607
18	22.547767962561764	33.167104101682	22.24049366460227	22.044634271153964
19	23.240754535652474	32.17296751713769	22.03856887201831	22.547709075191516
20	22.381970571625594	32.77679861167136	23.272877596123326	21.568353220579723
21	21.438889337087208	36.71397874283709	22.4213662223222	19.425765697753505
22	21.258487878329273	37.025169050918144	21.043696195345895	20.67264687540669
23	22.770362222103138	35.16556478582369	22.144742800577333	19.919330191495842
24	21.840618976926162	37.2704349480054	21.49492066987917	19.39402540518927
25	21.28121387059441	37.313750215175645	21.53227694252234	19.872758971707604
26	22.306913224412394	39.24857057503526	20.387863578683845	18.0566526218685
27	22.81164941922118	36.614693490725934	21.045558499995852	19.528098590057034
28	22.086835558104273	35.18851599994491	22.107933423400976	20.616715018549844
29	21.444550046275737	35.35724900879894	23.143615049718882	20.054585895206447
30	23.43925860215805	34.17677483103614	23.92759476089426	18.45637180591155
31	21.292699625133515	35.51360793670562	22.0705360478701	21.123156390290767
32	20.614918716606763	34.24918909876833	24.247562964389658	20.888329220235242
33	20.966726106151096	33.40308477810062	26.004600703522286	19.625588412225994
34	21.00298977353854	34.9967420002899	24.50967443032419	19.49059379584737
35	21.157228910112547	33.458211614260215	23.6239360919843	21.760623383642937
36	20.20152092823094	34.199883407418206	25.24470005595894	20.353895608391916
37	21.365617342899974	36.469905336221004	22.628654856394814	19.53582246448421
38	22.548489698824927	34.142456230591314	21.961110663882934	21.347943406700825
39	20.83876340431747	36.35333050595155	21.8807050853551	20.92720100437589
40	19.61993600674284	35.56002953534946	23.580293476547904	21.239740981359795
41	20.593061008474603	35.96842349297283	23.443847285858688	19.99466821269388
42	20.885088047930886	36.09577481527	22.986557119740983	20.032580017058134
43	21.21641234416393	34.02048787298694	22.817707165978963	21.94539261687016
44	21.424170295302382	33.010353190543036	22.860573758841575	22.704902755313007
45	20.66829249878495	32.91165770388045	26.19519108114952	20.224858716185075
46	21.21560654569463	33.727068847167565	24.78079712992704	20.276527477210763
47	20.80457973526738	34.937261787951485	23.234806573515552	21.023351903265585
48	22.043808611970345	32.67822948887907	23.961432709775618	21.316529189374968
49	21.012620046845733	31.771126196744632	22.80145303696642	24.414800719443214
50	19.35013430463949	34.952395685375556	25.648248414361458	20.049221595623496
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	293650.0
1	279284.5
2	169439.5
3	72390.0
4	67440.5
5	49545.0
6	25199.5
7	15185.0
8	13821.0
9	11259.5
10	9123.0
11	8174.0
12	7897.0
13	8178.5
14	9371.5
15	11028.0
16	13206.5
17	17350.0
18	23818.5
19	33222.0
20	44783.0
21	57352.0
22	65115.5
23	65988.0
24	65711.0
25	66666.0
26	67004.0
27	69057.0
28	73834.0
29	79284.0
30	85225.0
31	92454.0
32	102155.0
33	108847.0
34	121998.0
35	143268.5
36	155546.0
37	155960.0
38	154869.5
39	155940.0
40	157683.0
41	162720.5
42	168468.0
43	171238.5
44	171757.5
45	171681.5
46	180183.5
47	189147.0
48	198992.5
49	212671.5
50	231916.0
51	241322.0
52	227759.0
53	217822.0
54	211551.0
55	194657.5
56	177413.5
57	169108.5
58	162493.5
59	148588.5
60	128529.5
61	112100.0
62	100441.5
63	87357.0
64	72197.5
65	60672.5
66	45963.0
67	31136.0
68	22050.5
69	16193.0
70	12752.0
71	10177.0
72	7641.0
73	5207.0
74	3456.0
75	2288.0
76	1658.5
77	1296.0
78	985.0
79	825.5
80	580.5
81	326.5
82	192.5
83	147.0
84	117.5
85	87.5
86	66.0
87	46.5
88	21.5
89	5.0
90	4.0
91	4.0
92	3.5
93	3.0
94	2.0
95	1.0
96	1.0
97	1.0
98	0.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
24	38592.0
25	47125.0
26	54366.0
27	61595.0
28	62325.0
29	53694.0
30	43403.0
31	34232.0
32	27089.0
33	21161.0
34	22145.0
35	21311.0
36	20209.0
37	19540.0
38	21191.0
39	24601.0
40	32926.0
41	30835.0
42	38364.0
43	55089.0
44	123413.0
45	252992.0
46	377167.0
47	283589.0
48	555196.0
49	363094.0
50	711070.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	13.992769864172692
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.4283153976118	10.2746551887267
2	11.500443322507405	3.2184611349561534
3	4.00326555099862	1.6805032068088255
4	1.9069766770602878	1.067355431137975
5	1.0466221065258017	0.7322571135685589
6	0.7014230405353441	0.5888910710183606
7	0.545816671868711	0.5346240954241274
8	0.41847412602056405	0.46844897116132433
9	0.3506500369747931	0.44159087432267313
>10	3.282808719102042	10.494890031133755
>50	1.3602449778576688	13.924832071277041
>100	1.3697150081645464	31.327243263227494
>500	0.042469414707416046	4.023343343665255
>1k	0.03758084898569909	9.675356867837037
>5k	0.0021387475032511676	1.7350820476618178
>10k+	0.003055353576073097	9.81246528807288
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAA	33931	0.9990536799600979	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAA	33194	0.9773536840233265	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAA	30562	0.8998579047755891	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAA	27331	0.8047253581382641	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA	24923	0.7338249643584192	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGC	19564	0.576036255776115	No Hit
AAAAAAAAAAAAAAAAAAAAAAAA	19409	0.5714724845818143	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	17618	0.5187388445237985	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTA	12909	0.3800885312724324	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	10075	0.29664512762954187	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCT	7165	0.21096400391718786	No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	6100	0.17960647925957376	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAA	5619	0.1654440667146795	No Hit
AGTGCAGTGCTTATTGTGGGAAAAAAAAAAAAAAAAAAAAAAAAA	5589	0.1645607561609439	No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACACA	5570	0.16400132614357799	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAA	5461	0.1607919644650053	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	5080	0.14957392043256307	No Hit
AGTGCAGTGCTTATTGTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA	4950	0.14574624136637543	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAA	4699	0.13835587640012084	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	4503	0.13258491411571485	No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATCAT	4484	0.13202548409834897	No Hit
AGTGCAGTGCTTATTGTGGGAAAAAAAAAAAAAAAAAAAAAAAAAA	3987	0.11739197259146238	No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACA	3826	0.11265153928641462	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAA	3686	0.10852942336898178	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.1673284625626488	0.0	0.0	0.0	0.0
2	0.32155448524488606	0.0	0.0	0.0	0.0
3	0.4134776702036384	0.0	0.0	0.0	0.0
4	0.48708688301493913	0.0	0.0	0.0	0.0
5	0.5465925706515946	0.0	0.0	0.0	0.0
6	0.6512059838990152	0.0	0.0	0.0	0.0
7	0.7195447770730268	0.0	0.0	0.0	0.0
8	0.7914462561471054	0.0	0.0	0.0	0.0
9	0.8654382368650249	0.0	0.0	0.0	0.0
10	0.9417562687077814	0.0	0.0	0.0	0.0
11	1.0214897680249824	0.0	0.0	0.0	0.0
12	1.0966300524627581	0.0	0.0	0.0	0.0
13	1.1776296302403135	0.0	0.0	0.0	0.0
14	1.2417874201266432	0.0	0.0	0.0	0.0
15	1.3044141383864978	0.0	0.0	0.0	0.0
16	1.3524073451394658	0.0	0.0	0.0	0.0
17	1.3982217191932196	0.0	0.0	0.0	0.0
18	1.4358507487823564	0.0	0.0	0.0	0.0
19	1.474363088925229	0.0	0.0	0.0	0.0
20	1.5195885892764922	0.0	0.0	0.0	0.0
21	1.5670812533823433	0.0	0.0	0.0	0.0
22	1.6170471870386542	0.0	0.0	0.0	0.0
23	1.6618015884279251	0.0	0.0	0.0	0.0
24	1.7043182697477324	0.0	0.0	0.0	0.0
25	1.7494259953584974	0.0	0.0	0.0	0.0
26	1.7948576015056323	0.0	0.0	0.0	0.0
27	1.839376453413907	0.0	0.0	0.0	0.0
28	1.8884885202016068	0.0	0.0	0.0	0.0
29	1.9425765697753505	0.0	0.0	0.0	0.0
30	1.999579544176422	0.0	0.0	0.0	0.0
31	2.0514004299955775	0.0	0.0	0.0	0.0
32	2.1058712474759402	0.0	0.0	0.0	0.0
33	2.1594881980876917	0.0	0.0	0.0	0.0
34	2.205567565307566	0.0	0.0	0.0	0.0
35	2.244344898616559	0.0	0.0	0.0	0.0
36	2.2750546622014336	0.0	0.0	0.0	0.0
37	2.3017600846093735	0.0	0.0	0.0	0.0
38	2.3182191045939806	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTCGTAA	40	5.456968E-12	162.75473	44
CGTGCGT	30	2.491106E-8	155.00452	44
GCGATAT	65	1.8189894E-12	114.46487	44
TCGACGC	45	3.3287506E-10	94.90226	43
CTATCAT	1205	0.0	83.355125	44
GTCCGCA	60	3.6379788E-11	81.344795	43
TACGATT	30	3.9119186E-8	80.43323	42
TTCGACG	55	0.0	80.43323	42
TCGTGCG	325	0.0	75.087494	43
CCCCCGT	50	5.8595462E-5	74.40216	44
GTACGGG	30	1.1979137E-7	68.4383	41
CGTTAAC	20	8.86735E-5	68.43829	41
TCGTCTA	30	3.676516E-6	67.02769	42
ACCTTAG	2945	0.0	63.090237	42
ACGCCCC	45	0.0035983084	62.0018	44
TATCGAC	90	2.3704888E-7	62.0018	44
TCGCAAA	935	0.0	61.33484	43
CGCGATA	70	1.1008524E-8	61.008595	43
TTGCGAC	35	3.4794357E-7	58.661396	41
CGCAAAT	110	0.0	58.49689	42
>>END_MODULE