FastQCFastQC Report
Tue 24 May 2016
ERR841047.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR841047.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1506866
Sequences flagged as poor quality0
Sequence length24-50
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGC88830.5895016544271355No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTA56490.3748840308295495No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAGAA49420.3279654594370037No Hit
CACTTAGGAGATTTCAACTTAACTTGACCGCTCTGACCAAAAAAAAAAAA38170.25330719519851136No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCT33640.22324480079847844No Hit
AGCTTTAATTTATTAATGCAAACAGTACCTAACAAACCCACAGGTCCT32940.21859939769030556No Hit
TCAGTTCTTTGACCATTCGTTGGAGCTCCGGTTTTCAGCCTCTTTCCG29460.19550510795253193No Hit
CAGAGTGTTGCTTAACACAAAGCACCCAACTTACACTTAGGAGATTTCAA29450.1954387450509866No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACACA26520.17599441489820594No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAG26130.17340626173793822No Hit
CACTTAGGAGATTTCAACTTAACTTGACCGCTCTGACCAAAAAAAAAA22160.14706018982444358No Hit
CTTTTTCGCAACGGGTTTGCCGCCAGAACACAGGTGTCGTGAAAACTAC21570.14314477863326933No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCC20180.13392033531846895No Hit
AGCTTTAATTTATTAATGCAAACAGTACCTAACAAACCCACAGGTC19990.13265944018910772No Hit
AACACAAAGCACCCAACTTACACTTAGGAGATTTCAACTTAACTTGACCG19790.1313321821582012No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAA19310.12814676288402552No Hit
CCCGAAGCGTTTACTTTGAAAAAATTAGAGTGTTCAAAGCAGGCCC19210.12748313386857227No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAA18930.12562497262530312No Hit
CAGAGTGTTGCTTAACACAAAGCACCCAACTTACACTTAGGAGATTTC18390.12204137594185548No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAA18270.12124502112331155No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA16910.11221966651314716No Hit
TCAGTTCTTTGACCATTCGTTGGAGCTCCGGTTTTCAGCCTCTTTC16710.11089240848224063No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACA16550.10983060205751541No Hit
CTCTTTTTCCGGCTGGAACCATGGAGGG16080.10671154568488506No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATCAT15980.1060479166694318No Hit
ATAGGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACATGC15280.10140251356125893No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA15240.10113706195507763No Hit
CACTTAGGAGATTTCAACTTAACTTGACCGCTCTGACCAAAAAAAA15240.10113706195507763No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATATACG400.0304.3489744
CGAGATA205.2821633E-8304.3489744
CGCCCGA205.2821633E-8304.3489744
CCGATCT202.0124837E-5228.2617344
CACTCGA401.6719277E-6152.1744844
AAACTAT658.076313E-10140.4687544
ATAAGTC351.872688E-4130.4352744
GCTATAT100.009773159130.2349143
CCTGATA301.371518E-9130.2349143
CTATCAT4500.0118.3579444
GGTCATC651.3273166E-7117.0572944
GTTCATC651.3273166E-7117.0572944
ACGCCCC403.186352E-4114.1308744
GTTCGGC1200.0113.9555443
ATGCGAC855.2241376E-9107.4172844
TAGGCAG4900.0105.5904644
ACATTGG1650.0101.4496644
TGTACTC205.923688E-497.6761843
CGATGAA501.7702798E-491.3046944
GTACGTA202.6643153E-587.2188342
Produced by FastQC (version 0.11.5)