##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR841047.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1506866
Sequences flagged as poor quality	0
Sequence length	24-50
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	35.96251292417507	38.0	35.0	39.0	30.0	39.0
2	37.149707405967085	39.0	37.0	39.0	34.0	39.0
3	37.430946746426024	39.0	37.0	39.0	34.0	39.0
4	37.94359352457352	39.0	37.0	40.0	34.0	41.0
5	38.36782699987922	40.0	37.0	41.0	34.0	41.0
6	38.29490080737106	40.0	37.0	41.0	34.0	41.0
7	38.34297542050852	40.0	37.0	41.0	34.0	41.0
8	38.31477516912585	40.0	37.0	41.0	34.0	41.0
9	38.340910207012435	40.0	37.0	41.0	34.0	41.0
10	38.277074404757954	40.0	37.0	41.0	34.0	41.0
11	38.16893008402871	40.0	37.0	41.0	34.0	41.0
12	38.135495790601155	39.0	37.0	40.0	34.0	41.0
13	38.03462816202635	39.0	37.0	40.0	34.0	41.0
14	37.99843051737845	39.0	37.0	40.0	34.0	41.0
15	37.89236269183856	39.0	36.0	40.0	34.0	41.0
16	37.802604876611454	39.0	36.0	40.0	34.0	41.0
17	37.91306393534661	39.0	36.0	40.0	34.0	41.0
18	37.928349302459544	39.0	36.0	40.0	34.0	41.0
19	37.96196343935028	39.0	36.0	40.0	34.0	41.0
20	37.884427679700785	39.0	36.0	40.0	34.0	41.0
21	37.76242081246773	39.0	36.0	40.0	33.0	41.0
22	37.65540200654869	39.0	35.0	40.0	33.0	41.0
23	37.61672968930217	39.0	35.0	40.0	33.0	41.0
24	37.439007848076734	39.0	35.0	40.0	33.0	41.0
25	37.334268727070004	38.0	35.0	40.0	33.0	41.0
26	37.0838995771329	38.0	35.0	40.0	33.0	41.0
27	36.94705596153221	38.0	35.0	40.0	32.0	41.0
28	36.848402701023446	38.0	35.0	40.0	32.0	41.0
29	36.76642041305234	38.0	35.0	40.0	32.0	41.0
30	36.72983792472454	38.0	35.0	40.0	32.0	41.0
31	36.654188915807964	38.0	35.0	40.0	32.0	41.0
32	36.486052994168396	38.0	35.0	40.0	31.0	41.0
33	36.41380010848716	38.0	34.0	40.0	31.0	41.0
34	36.32755301554501	37.0	34.0	40.0	31.0	41.0
35	36.17972788801033	37.0	34.0	40.0	31.0	41.0
36	36.14122247118402	37.0	34.0	40.0	31.0	41.0
37	36.04660644423931	37.0	34.0	40.0	31.0	41.0
38	35.875127809128536	36.0	34.0	39.0	31.0	41.0
39	35.77515508951984	36.0	34.0	39.0	31.0	41.0
40	35.59700939448997	36.0	34.0	39.0	30.0	41.0
41	35.46399351645909	36.0	34.0	39.0	30.0	41.0
42	35.24580941525605	35.0	33.0	39.0	29.0	41.0
43	35.06235156928267	35.0	33.0	39.0	29.0	41.0
44	34.79265361457794	35.0	33.0	39.0	29.0	40.0
45	34.55328251100337	35.0	32.0	38.0	28.0	40.0
46	34.26272279998822	35.0	32.0	38.0	28.0	40.0
47	34.46631478408318	35.0	33.0	38.0	28.0	40.0
48	35.1880926283815	35.0	34.0	39.0	30.0	40.0
49	35.55355198542319	36.0	34.0	39.0	31.0	40.0
50	35.91072072778606	36.0	34.0	39.0	31.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	4.0
20	10.0
21	28.0
22	62.0
23	127.0
24	258.0
25	548.0
26	957.0
27	1945.0
28	3954.0
29	8082.0
30	15668.0
31	30268.0
32	54264.0
33	89811.0
34	133284.0
35	133847.0
36	205860.0
37	258671.0
38	228698.0
39	218412.0
40	122107.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	42.96208156531503	20.71013613685623	36.32778229782873
2	23.34918964260923	22.321029208967484	30.68102936823845	23.648751780184835
3	21.19465168103866	22.988839087218107	28.66990163690733	27.146607594835903
4	18.8880099491262	24.792649114121627	34.61130584935887	21.708035087393306
5	18.614860246365637	24.801873557436426	33.85795419101632	22.725312005181618
6	21.392944030856096	25.940063681840325	29.847312236124512	22.81968005117907
7	27.84587348841901	25.171514919043897	24.198302967881684	22.78430862465541
8	25.74734581575269	24.544916402652923	27.918408139808054	21.78932964178633
9	25.229582457896054	25.970988793960448	25.444000992789007	23.35542775535449
10	25.280217351775143	21.778711577539077	26.495985708085524	26.445085362600256
11	24.025892149666923	24.384649995420958	26.489349417930992	25.100108436981127
12	23.72480366535578	25.613823657843497	27.27263074487048	23.388741931930245
13	23.447008559487042	25.145699750342764	27.176139086023575	24.23115260414662
14	22.398474715070883	26.962384180146078	24.81023528303114	25.828905821751903
15	22.057236675324813	29.189987696318052	24.49640512162329	24.256370506733845
16	22.635390273587696	31.139928832424385	23.499103437200123	22.725577456787796
17	22.97503560369668	28.795592972434175	23.10285055207298	25.126520871796167
18	21.96890765336798	27.484527489504707	23.35290596509577	27.193658892031543
19	23.219118355580388	26.169214780876338	24.361953883092458	26.249712980450816
20	25.070776034498092	26.847310908866483	24.917345006125295	23.16456805051013
21	24.54398732203129	29.98156438595071	22.41552998076803	23.058918311249972
22	26.374873412765304	29.08752337633207	21.250330155435186	23.28727305546744
23	27.115947934321966	26.660499341016386	22.773756923309705	23.449795801351943
24	25.392569744091382	28.064738337715493	22.598558863230043	23.944133054963082
25	26.086802669310604	26.410351423621197	24.566070111132255	22.936775795935947
26	26.274653797733947	30.83910337110085	23.05587387154739	19.830368959617807
27	26.078518534580898	29.66671364912775	22.58620829854824	21.66855951774311
28	26.416318239016924	27.34509409084478	23.372642944194844	22.865944725943454
29	24.81155150810513	27.684671246942514	24.074307769436	23.429469475516353
30	26.97205953513207	27.642552824637225	25.907780058178016	19.477607582052695
31	25.13587899739497	29.125052153272772	24.923198827559368	20.815870021772895
32	23.32579020779296	27.3222328499936	26.70881485267576	22.64316208953768
33	23.878936312619796	25.361760485725952	28.92423077114983	21.835072430504425
34	23.163461159005376	27.776737377977245	25.759653295735436	23.300148167281936
35	21.162603823268842	28.00550929947205	26.340723868765366	24.491163008493743
36	22.299732707686417	27.937395526401392	25.490721179841223	24.27215058607096
37	22.186689524814277	28.86353978929477	23.72492563775697	25.224845048133982
38	23.43040806773239	28.56710025322241	23.82015679885589	24.18233488018931
39	23.1185837186443	30.698890245025108	23.951568119118257	22.230957917212336
40	22.357523639692975	29.305583317050747	24.49080426985521	23.84608877340107
41	23.27497440906486	30.770585771591264	23.406293328817434	22.54814649052644
42	21.680040913959136	32.524609914602046	24.059349703503756	21.73599946793506
43	22.882052228991988	27.64711952275621	25.678775949432893	23.792052298818916
44	23.00681845368635	29.332908010686232	23.947605566410143	23.712667969217275
45	22.758109176368215	27.41785050835177	28.099421290051275	21.72461902522874
46	21.909082874230325	29.76774789107229	24.980604739317876	23.34256449537951
47	21.53710335948351	31.365986264450722	24.478152919649716	22.618757456416045
48	23.020196641753966	29.336968145459725	24.04463818243573	23.598197030350583
49	20.116436681998977	30.025331644557028	21.084594360375974	28.77363731306802
50	21.351527984733224	32.543329934907064	24.496902203748725	21.608239876610984
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	30259.0
1	27340.5
2	22595.0
3	19620.5
4	16759.5
5	10892.0
6	4991.0
7	3429.0
8	3726.5
9	3772.0
10	3456.5
11	2848.0
12	2299.5
13	2028.5
14	2046.5
15	2311.5
16	2785.5
17	3252.5
18	3321.5
19	3328.0
20	3524.0
21	4010.5
22	4993.5
23	6484.5
24	7912.5
25	8820.5
26	10284.5
27	12869.0
28	15595.0
29	18357.5
30	22141.0
31	26944.5
32	32151.5
33	38224.0
34	50727.5
35	61092.5
36	61401.5
37	61263.0
38	60354.0
39	60509.0
40	66242.5
41	77578.0
42	88245.5
43	91423.0
44	94200.0
45	93884.5
46	95428.5
47	103676.5
48	116829.5
49	129263.5
50	141048.5
51	150442.0
52	147087.5
53	139043.5
54	131707.0
55	127028.5
56	120620.0
57	113107.5
58	106849.5
59	97134.0
60	82967.5
61	67224.0
62	55141.0
63	44683.0
64	33880.5
65	26120.0
66	18960.5
67	12864.5
68	9629.0
69	7704.5
70	5942.0
71	4614.0
72	4024.5
73	3146.5
74	2214.0
75	1602.5
76	1235.5
77	841.0
78	484.5
79	262.5
80	199.0
81	128.0
82	65.0
83	35.0
84	13.5
85	10.5
86	7.0
87	7.5
88	7.5
89	7.0
90	4.5
91	1.5
92	1.0
93	1.0
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
24	9101.0
25	10773.0
26	11264.0
27	12727.0
28	12870.0
29	11787.0
30	12259.0
31	11552.0
32	11602.0
33	9236.0
34	9853.0
35	9691.0
36	9211.0
37	8425.0
38	9551.0
39	11181.0
40	13049.0
41	14623.0
42	19210.0
43	34951.0
44	86353.0
45	149307.0
46	160807.0
47	183262.0
48	224191.0
49	258765.0
50	191265.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	12.220240678383707
#Duplication Level	Percentage of deduplicated	Percentage of total
1	66.73361792523606	8.15500872385686
2	11.822553600631654	2.889489008656214
3	4.740205139790028	1.7377934301943687
4	2.3923176494994935	1.1693878982411605
5	1.5277694898169516	0.9334855433327318
6	1.1143515714423016	0.8170586642016013
7	0.8464437689647081	0.7240622604226863
8	0.6325659219260262	0.6184086248703775
9	0.5280743218603645	0.5807875778287126
>10	5.552847815733952	16.00999233474179
>50	2.383515985860987	20.950058030957432
>100	1.6355435072899853	32.97769202667409
>500	0.05744796302388428	4.833321924373353
>1k	0.03159637966313635	6.583266957037404
>5k	0.0011489592604776854	1.020186994611224
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGC	8883	0.5895016544271355	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTA	5649	0.3748840308295495	No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAGAA	4942	0.3279654594370037	No Hit
CACTTAGGAGATTTCAACTTAACTTGACCGCTCTGACCAAAAAAAAAAAA	3817	0.25330719519851136	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCT	3364	0.22324480079847844	No Hit
AGCTTTAATTTATTAATGCAAACAGTACCTAACAAACCCACAGGTCCT	3294	0.21859939769030556	No Hit
TCAGTTCTTTGACCATTCGTTGGAGCTCCGGTTTTCAGCCTCTTTCCG	2946	0.19550510795253193	No Hit
CAGAGTGTTGCTTAACACAAAGCACCCAACTTACACTTAGGAGATTTCAA	2945	0.1954387450509866	No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACACA	2652	0.17599441489820594	No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAG	2613	0.17340626173793822	No Hit
CACTTAGGAGATTTCAACTTAACTTGACCGCTCTGACCAAAAAAAAAA	2216	0.14706018982444358	No Hit
CTTTTTCGCAACGGGTTTGCCGCCAGAACACAGGTGTCGTGAAAACTAC	2157	0.14314477863326933	No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCC	2018	0.13392033531846895	No Hit
AGCTTTAATTTATTAATGCAAACAGTACCTAACAAACCCACAGGTC	1999	0.13265944018910772	No Hit
AACACAAAGCACCCAACTTACACTTAGGAGATTTCAACTTAACTTGACCG	1979	0.1313321821582012	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAA	1931	0.12814676288402552	No Hit
CCCGAAGCGTTTACTTTGAAAAAATTAGAGTGTTCAAAGCAGGCCC	1921	0.12748313386857227	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAA	1893	0.12562497262530312	No Hit
CAGAGTGTTGCTTAACACAAAGCACCCAACTTACACTTAGGAGATTTC	1839	0.12204137594185548	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAA	1827	0.12124502112331155	No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1691	0.11221966651314716	No Hit
TCAGTTCTTTGACCATTCGTTGGAGCTCCGGTTTTCAGCCTCTTTC	1671	0.11089240848224063	No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACA	1655	0.10983060205751541	No Hit
CTCTTTTTCCGGCTGGAACCATGGAGGG	1608	0.10671154568488506	No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATCAT	1598	0.1060479166694318	No Hit
ATAGGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACATGC	1528	0.10140251356125893	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA	1524	0.10113706195507763	No Hit
CACTTAGGAGATTTCAACTTAACTTGACCGCTCTGACCAAAAAAAA	1524	0.10113706195507763	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.052957595433170565	0.0	0.0	0.0	0.0
2	0.1083042553219729	0.0	0.0	0.0	0.0
3	0.14228206091318008	0.0	0.0	0.0	0.0
4	0.1716144633962144	0.0	0.0	0.0	0.0
5	0.19709781758961978	0.0	0.0	0.0	0.0
6	0.23220379250709752	0.0	0.0	0.0	0.0
7	0.25755442089741226	0.0	0.0	0.0	0.0
8	0.28589137985726665	0.0	0.0	0.0	0.0
9	0.31183927436148934	0.0	0.0	0.0	0.0
10	0.3332081286590845	0.0	0.0	0.0	0.0
11	0.3597532892772151	0.0	0.0	0.0	0.0
12	0.3827148532118981	0.0	0.0	0.0	0.0
13	0.4083972961099394	0.0	0.0	0.0	0.0
14	0.4332833841894369	0.0	0.0	0.0	0.0
15	0.4566431255333918	0.0	0.0	0.0	0.0
16	0.4813964878097986	0.0	0.0	0.0	0.0
17	0.5074107452155666	0.0	0.0	0.0	0.0
18	0.5282486963007992	0.0	0.0	0.0	0.0
19	0.5527366069710247	0.0	0.0	0.0	0.0
20	0.5754327192995263	0.0	0.0	0.0	0.0
21	0.5989915493481173	0.0	0.0	0.0	0.0
22	0.6223512906920722	0.0	0.0	0.0	0.0
23	0.6453128546267551	0.0	0.0	0.0	0.0
24	0.6717252894417951	0.0	0.0	0.0	0.0
25	0.6956159339981126	0.0	0.0	0.0	0.0
26	0.7232892639425138	0.0	0.0	0.0	0.0
27	0.7460517391725608	0.0	0.0	0.0	0.0
28	0.7737914320185073	0.0	0.0	0.0	0.0
29	0.8040529151231762	0.0	0.0	0.0	0.0
30	0.8327880514923026	0.0	0.0	0.0	0.0
31	0.8644431555294233	0.0	0.0	0.0	0.0
32	0.894041009618639	0.0	0.0	0.0	0.0
33	0.9187280089935004	0.0	0.0	0.0	0.0
34	0.9432159196637259	0.0	0.0	0.0	0.0
35	0.9649165884690477	0.0	0.0	0.0	0.0
36	0.9874799749944587	0.0	0.0	0.0	0.0
37	1.003805248774609	0.0	0.0	0.0	0.0
38	1.0180069097053088	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATATACG	40	0.0	304.34897	44
CGAGATA	20	5.2821633E-8	304.34897	44
CGCCCGA	20	5.2821633E-8	304.34897	44
CCGATCT	20	2.0124837E-5	228.26173	44
CACTCGA	40	1.6719277E-6	152.17448	44
AAACTAT	65	8.076313E-10	140.46875	44
ATAAGTC	35	1.872688E-4	130.43527	44
GCTATAT	10	0.009773159	130.23491	43
CCTGATA	30	1.371518E-9	130.23491	43
CTATCAT	450	0.0	118.35794	44
GGTCATC	65	1.3273166E-7	117.05729	44
GTTCATC	65	1.3273166E-7	117.05729	44
ACGCCCC	40	3.186352E-4	114.13087	44
GTTCGGC	120	0.0	113.95554	43
ATGCGAC	85	5.2241376E-9	107.41728	44
TAGGCAG	490	0.0	105.59046	44
ACATTGG	165	0.0	101.44966	44
TGTACTC	20	5.923688E-4	97.67618	43
CGATGAA	50	1.7702798E-4	91.30469	44
GTACGTA	20	2.6643153E-5	87.21883	42
>>END_MODULE