FastQCFastQC Report
Tue 24 May 2016
ERR841046.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR841046.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2002295
Sequences flagged as poor quality0
Sequence length24-50
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGC266181.3293745427122377No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTA169440.8462289522772618No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCT102850.5136605744907718No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAA41960.20955953043882147No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATCAT40270.20111921569998426No Hit
ATAGGTGTATGAACATGAGGGTGTTTTCTCGTGTGAATGAGGGTTTTATG39120.19537580626231402No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAA38800.1937776401579188No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAA38410.19182987521818715No Hit
TCAGTTCTTTGACCATTCGTTGGAGCTCCGGTTTTCAGCCTCTTTCCG31700.15831832971665014No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACC31640.15801867357207605No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA31210.155871137869295No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAA30520.1524250922066928No Hit
AAGATAAAATTTGAAATCTGGTTAGGCTGGTGTTAGGGTTCTTTGTTTTT27620.13794171188561127No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA24720.1234583315645297No Hit
AAAAAAAAAAAAAAAAAAAAAAAA24270.12121091048022395No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATC23860.11916326015896758No Hit
CTCTTTTCCGTGGCGCCTCGGAGGCGTTCAGCTGCTTCAAGATGAAGCT22320.11147208578156564No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGCC21510.10742672782981529No Hit
CTCTCGGCCTTAGCGCCATTTTTTTGGAAACCTCTGCGCCATGAGAGCCA20810.10393073947645078No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTT20580.10278205758891672No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCTTA353.6379788E-12173.3601744
TACCGGC156.033992E-5173.3601744
TCGTAGG1300.0146.6893844
CGCTAAA207.39407E-6112.90340443
TGTCGCG254.613136E-4104.01610644
CTTACGG254.613136E-4104.01610644
AATTACG309.521824E-486.68008444
GTATAGC309.521824E-486.68008444
CGGTAAT507.9659003E-786.68008444
TCCCGTA200.001045050984.6775543
CAGGTCG1050.082.5524744
CACGACT551.404258E-678.8000844
CCGCACG551.404258E-678.8000844
CGAGAAA2900.077.7131844
CGTGCGC1700.076.4824344
ACGCTAA306.049413E-875.5276442
CGTCTTA205.440675E-575.5276342
ACGTCCA451.8189894E-1275.5276342
CGTCCAA750.075.2689443
CCTTAGC47050.073.1892443
Produced by FastQC (version 0.11.5)