##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR841046.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2002295
Sequences flagged as poor quality	0
Sequence length	24-50
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	35.00782052594648	35.0	34.0	39.0	27.0	39.0
2	35.92020806124972	37.0	35.0	39.0	33.0	39.0
3	36.30796211347479	37.0	35.0	39.0	33.0	39.0
4	36.460864158378264	37.0	35.0	39.0	34.0	39.0
5	37.298306693069705	38.0	35.0	41.0	34.0	41.0
6	37.247685281139894	38.0	35.0	41.0	34.0	41.0
7	37.28230805151089	38.0	35.0	41.0	34.0	41.0
8	37.224201728516526	38.0	35.0	41.0	34.0	41.0
9	37.27633190913427	38.0	35.0	41.0	34.0	41.0
10	37.23309802002203	38.0	35.0	41.0	34.0	41.0
11	37.171796363672684	38.0	35.0	41.0	34.0	41.0
12	37.123028824423976	38.0	35.0	40.0	34.0	41.0
13	37.00350697574533	38.0	35.0	40.0	33.0	41.0
14	36.953883918203864	37.0	35.0	40.0	33.0	41.0
15	36.90289093265478	37.0	35.0	40.0	33.0	41.0
16	36.884093502705646	37.0	35.0	40.0	33.0	41.0
17	36.87269608124677	37.0	35.0	40.0	33.0	41.0
18	36.843615950696574	37.0	35.0	40.0	33.0	41.0
19	36.83863466671994	36.0	35.0	40.0	33.0	41.0
20	36.81330772938053	36.0	35.0	40.0	33.0	41.0
21	36.7239217997348	36.0	35.0	40.0	33.0	41.0
22	36.61338064570905	36.0	35.0	40.0	33.0	41.0
23	36.57379007588792	36.0	35.0	40.0	33.0	41.0
24	36.45460234381048	36.0	35.0	40.0	33.0	41.0
25	36.378299472365775	36.0	35.0	40.0	33.0	41.0
26	36.2745774188504	35.0	35.0	40.0	32.0	41.0
27	36.16644689272278	35.0	35.0	40.0	32.0	41.0
28	36.076956572926235	35.0	34.0	40.0	32.0	41.0
29	36.03269961464594	35.0	34.0	40.0	32.0	41.0
30	36.009675505191645	35.0	34.0	40.0	32.0	41.0
31	35.964161208521915	35.0	34.0	40.0	32.0	41.0
32	35.86088866822696	35.0	34.0	40.0	31.0	41.0
33	35.8218926763898	35.0	34.0	40.0	31.0	41.0
34	35.79169591810884	35.0	34.0	39.0	31.0	41.0
35	35.69838376670334	35.0	34.0	39.0	31.0	41.0
36	35.6786907085928	35.0	34.0	39.0	31.0	41.0
37	35.66129379517769	35.0	34.0	39.0	31.0	41.0
38	35.53479325713903	35.0	34.0	39.0	31.0	41.0
39	35.37185028760016	35.0	34.0	39.0	31.0	41.0
40	35.2700751586683	35.0	34.0	39.0	30.0	41.0
41	35.14464111122349	35.0	34.0	39.0	30.0	41.0
42	35.07001722134676	35.0	34.0	38.0	30.0	41.0
43	34.92871204123633	35.0	33.0	38.0	30.0	41.0
44	34.69229280590556	35.0	33.0	38.0	29.0	40.0
45	34.55994478182085	35.0	33.0	38.0	29.0	40.0
46	34.279679223881445	35.0	32.0	38.0	28.0	40.0
47	34.13729279032889	35.0	32.0	37.0	28.0	40.0
48	34.88674985089322	35.0	34.0	38.0	30.0	40.0
49	35.62903255574911	35.0	34.0	39.0	32.0	40.0
50	35.64720516955792	36.0	34.0	39.0	31.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	2.0
20	8.0
21	31.0
22	102.0
23	255.0
24	664.0
25	1506.0
26	2935.0
27	5967.0
28	11084.0
29	19553.0
30	35422.0
31	64995.0
32	124740.0
33	228175.0
34	396220.0
35	120542.0
36	138535.0
37	212120.0
38	214787.0
39	212424.0
40	212228.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	45.58189477574483	18.9089519776057	35.509153246649475
2	22.58063871707216	21.78220491985447	35.10451756609291	20.532638796980464
3	21.831148756801568	24.89528266314404	27.702711138968034	25.570857441086353
4	19.071865034872484	26.566115382598472	32.72749519925885	21.634524383270197
5	19.12090875720111	27.67294529527367	31.185264908517475	22.02088103900774
6	20.799132994888367	28.41913903795395	26.764687521069575	24.017040446088114
7	24.482805980137794	26.886497743838945	23.491992938103525	25.138703337919736
8	24.158578031708615	25.50787970803503	28.71425039766867	21.61929186258768
9	23.421623686819377	29.404258613241307	25.621249616065565	21.552868083873754
10	27.590489912825035	23.570852446817277	26.941734359822107	21.896923280535585
11	25.54873282907863	24.433312773592302	26.255671616819697	23.762282780509366
12	23.99721319785546	26.29417743139747	25.843944074174885	23.86466529657218
13	24.119822503677028	24.150687086568162	26.165674888065944	25.56381552168886
14	19.745342219802776	25.698161359839585	26.924104589982996	27.632391830374647
15	22.238031858442437	31.42209314811254	26.370090321356244	19.969784672088778
16	22.005498690252935	34.85100846778322	22.226045612659473	20.917447229304372
17	22.16456616033102	34.97211949288192	21.31688887002165	21.546425476765414
18	21.330573167290535	27.612914180977327	23.626288833563486	27.430223818168653
19	22.61849527667002	24.956462459327923	23.414631710112648	29.01041055388941
20	22.714335300242972	25.652763453936604	24.931690884709795	26.70121036111063
21	23.820316187175216	29.883209017652245	24.171962672832926	22.124512122339617
22	23.39710182565506	27.354410813591407	23.648613216334258	25.599874144419278
23	25.028379934025708	26.882801984722533	27.074731745322246	21.014086335929523
24	23.89557982215408	27.569713753467894	25.113232565630938	23.42147385874709
25	23.785642440642405	27.131160966120277	24.246706933401125	24.836489659836193
26	25.22060858972951	31.513336717255115	23.20300848527665	20.063046207738722
27	24.498114839279467	27.24008799116417	24.533248075320763	23.7285490942356
28	24.309568083869245	26.562587558730222	23.315183667048306	25.812660690352228
29	23.416843995901722	27.525386079966154	27.37138963899501	21.686380285137112
30	24.845566964859366	28.355817056009595	26.201433121850876	20.59718285728016
31	24.284310800364768	30.765150594278694	24.00536526815027	20.94517333720626
32	22.348378673530974	27.971054194699065	25.907417720271443	23.773149411498522
33	22.785715009717265	26.201454361938843	30.326503241666174	20.686327386677718
34	22.763525974207205	31.14387630450698	25.25209912642849	20.84049859485733
35	22.683270227488876	26.318458500008912	25.260375740352796	25.73789553214942
36	22.402414117414388	27.749745043077805	25.025821689349726	24.82201915015808
37	21.946550702843066	32.9757551382626	23.217701022297316	21.85999313659702
38	26.433698698937974	27.91510813426156	23.423182829520044	22.228010337280423
39	22.54088731767587	31.868585659517894	23.241465282058225	22.34906174074801
40	20.795846787662846	30.470938648257434	23.999331922948446	24.733882641131277
41	22.61061101558725	33.003101716058126	23.031084589191195	21.355202679163433
42	21.584465970970378	33.02858108464431	23.15990448588581	22.227048458499503
43	22.808373650079506	26.9109048173088	23.835095954524892	26.445625578086805
44	22.48453421999323	27.816069911581515	23.27645933176605	26.422936536659204
45	21.060225770116066	27.563054018316276	29.235883245996376	22.140836965571282
46	21.807223436151936	28.196204529394635	28.270194280795856	21.726377753657573
47	22.525292037208064	30.75760059600424	25.806480409931453	20.91062695685624
48	24.085241742324552	29.213094888714274	24.618428651222676	22.083234717738502
49	19.870492545725842	28.083610534938757	25.44121551306787	26.604681406267527
50	21.45405819462661	30.07980865128399	26.414328457229153	22.05180469686025
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	54390.0
1	47118.5
2	32060.0
3	23328.0
4	20808.5
5	14548.5
6	7232.0
7	4735.5
8	4876.0
9	4668.0
10	4188.5
11	3783.0
12	3597.5
13	3678.0
14	4237.0
15	4739.0
16	4707.5
17	4554.0
18	4600.5
19	5083.0
20	5842.5
21	7469.5
22	9740.0
23	12566.5
24	15235.0
25	17783.5
26	20865.0
27	24397.0
28	28019.0
29	33111.5
30	39893.0
31	48423.5
32	58810.5
33	64378.5
34	69912.5
35	77928.5
36	80096.0
37	80263.5
38	83014.5
39	90465.5
40	100489.5
41	110456.0
42	116213.0
43	112842.5
44	110848.0
45	113901.5
46	120335.5
47	128035.0
48	143403.5
49	161531.5
50	180180.0
51	193575.5
52	180751.5
53	156750.5
54	143417.0
55	137845.5
56	134509.0
57	134942.0
58	133739.5
59	119941.5
60	102332.5
61	90254.5
62	76952.5
63	62190.0
64	48110.5
65	39324.5
66	31200.5
67	22914.5
68	17548.5
69	13276.0
70	10220.0
71	6893.0
72	4379.5
73	3147.5
74	2152.0
75	1337.5
76	836.0
77	553.0
78	373.5
79	333.0
80	277.0
81	242.5
82	166.5
83	138.0
84	159.0
85	143.5
86	120.5
87	61.5
88	15.5
89	13.5
90	7.5
91	2.5
92	1.5
93	0.5
94	0.5
95	1.0
96	1.0
97	1.0
98	1.5
99	2.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
24	11901.0
25	14383.0
26	14905.0
27	15983.0
28	16507.0
29	14194.0
30	14051.0
31	13120.0
32	12752.0
33	11343.0
34	11505.0
35	11144.0
36	10511.0
37	10175.0
38	10886.0
39	12735.0
40	16540.0
41	16151.0
42	19610.0
43	33510.0
44	76869.0
45	160343.0
46	240971.0
47	175916.0
48	351929.0
49	246140.0
50	458221.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	12.05717474675237
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.84862923709464	8.301199537860143
2	11.247207334258649	2.7121908848422285
3	4.261958149879867	1.541615225293409
4	2.116683288619148	1.0208488117764618
5	1.339931672357713	0.8077895161162543
6	0.8913901633012848	0.6448588179875834
7	0.7295042732779969	0.6157032350990782
8	0.5789989534178063	0.5584873247636181
9	0.4746316774348891	0.5150445367658996
>10	5.2849713353954195	15.613014489389892
>50	2.530570687805652	21.87391673819864
>100	1.6074323042676932	31.49520553287762
>500	0.05267292296470407	4.223380882288941
>1k	0.03405576915821384	7.128683249377065
>5k	0.0	0.0
>10k+	0.0013622307663285534	2.9480612173631746
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGC	26618	1.3293745427122377	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTA	16944	0.8462289522772618	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCT	10285	0.5136605744907718	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAA	4196	0.20955953043882147	No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATCAT	4027	0.20111921569998426	No Hit
ATAGGTGTATGAACATGAGGGTGTTTTCTCGTGTGAATGAGGGTTTTATG	3912	0.19537580626231402	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAA	3880	0.1937776401579188	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAA	3841	0.19182987521818715	No Hit
TCAGTTCTTTGACCATTCGTTGGAGCTCCGGTTTTCAGCCTCTTTCCG	3170	0.15831832971665014	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACC	3164	0.15801867357207605	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA	3121	0.155871137869295	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3052	0.1524250922066928	No Hit
AAGATAAAATTTGAAATCTGGTTAGGCTGGTGTTAGGGTTCTTTGTTTTT	2762	0.13794171188561127	No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2472	0.1234583315645297	No Hit
AAAAAAAAAAAAAAAAAAAAAAAA	2427	0.12121091048022395	No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATC	2386	0.11916326015896758	No Hit
CTCTTTTCCGTGGCGCCTCGGAGGCGTTCAGCTGCTTCAAGATGAAGCT	2232	0.11147208578156564	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGCC	2151	0.10742672782981529	No Hit
CTCTCGGCCTTAGCGCCATTTTTTTGGAAACCTCTGCGCCATGAGAGCCA	2081	0.10393073947645078	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTT	2058	0.10278205758891672	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.05508678791087227	0.0	0.0	0.0	0.0
2	0.09234403521958552	0.0	0.0	0.0	0.0
3	0.11581709987789011	0.0	0.0	0.0	0.0
4	0.14438431899395443	0.0	0.0	0.0	0.0
5	0.1734010223268799	0.0	0.0	0.0	0.0
6	0.2138046591536212	0.0	0.0	0.0	0.0
7	0.24272147710502198	0.0	0.0	0.0	0.0
8	0.2779810167832412	0.0	0.0	0.0	0.0
9	0.3070976054976914	0.0	0.0	0.0	0.0
10	0.33531522577841927	0.0	0.0	0.0	0.0
11	0.3628336483884742	0.0	0.0	0.0	0.0
12	0.3917005236491126	0.0	0.0	0.0	0.0
13	0.4166219263395254	0.0	0.0	0.0	0.0
14	0.4446397758572039	0.0	0.0	0.0	0.0
15	0.4736564791901293	0.0	0.0	0.0	0.0
16	0.5015744433262831	0.0	0.0	0.0	0.0
17	0.5261462471813594	0.0	0.0	0.0	0.0
18	0.5522662744500686	0.0	0.0	0.0	0.0
19	0.5753897402730367	0.0	0.0	0.0	0.0
20	0.5994621172204895	0.0	0.0	0.0	0.0
21	0.6209374742483	0.0	0.0	0.0	0.0
22	0.639466212521132	0.0	0.0	0.0	0.0
23	0.663139047942486	0.0	0.0	0.0	0.0
24	0.6861126856931671	0.0	0.0	0.0	0.0
25	0.7090863234438481	0.0	0.0	0.0	0.0
26	0.7352562934033197	0.0	0.0	0.0	0.0
27	0.7597282118768713	0.0	0.0	0.0	0.0
28	0.7847994426395711	0.0	0.0	0.0	0.0
29	0.8114188968159037	0.0	0.0	0.0	0.0
30	0.8374390387030882	0.0	0.0	0.0	0.0
31	0.8631095817549362	0.0	0.0	0.0	0.0
32	0.8897789786220313	0.0	0.0	0.0	0.0
33	0.9152497509108298	0.0	0.0	0.0	0.0
34	0.9400213255289556	0.0	0.0	0.0	0.0
35	0.9619961094643896	0.0	0.0	0.0	0.0
36	0.9837711226367744	0.0	0.0	0.0	0.0
37	1.0035983708694274	0.0	0.0	0.0	0.0
38	1.017981865808984	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGCTTA	35	3.6379788E-12	173.36017	44
TACCGGC	15	6.033992E-5	173.36017	44
TCGTAGG	130	0.0	146.68938	44
CGCTAAA	20	7.39407E-6	112.903404	43
TGTCGCG	25	4.613136E-4	104.016106	44
CTTACGG	25	4.613136E-4	104.016106	44
AATTACG	30	9.521824E-4	86.680084	44
GTATAGC	30	9.521824E-4	86.680084	44
CGGTAAT	50	7.9659003E-7	86.680084	44
TCCCGTA	20	0.0010450509	84.67755	43
CAGGTCG	105	0.0	82.55247	44
CACGACT	55	1.404258E-6	78.80008	44
CCGCACG	55	1.404258E-6	78.80008	44
CGAGAAA	290	0.0	77.71318	44
CGTGCGC	170	0.0	76.48243	44
ACGCTAA	30	6.049413E-8	75.52764	42
CGTCTTA	20	5.440675E-5	75.52763	42
ACGTCCA	45	1.8189894E-12	75.52763	42
CGTCCAA	75	0.0	75.26894	43
CCTTAGC	4705	0.0	73.18924	43
>>END_MODULE