FastQCFastQC Report
Tue 24 May 2016
ERR841045.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR841045.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1858401
Sequences flagged as poor quality0
Sequence length24-50
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGC109830.5909919333878964No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTA73240.39410224165828583No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAA57680.3103743487008455No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAA55270.2974062110384142No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAA50220.270232312617137No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAA46030.2476860483824535No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCT42990.23132789962984307No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA41450.22304120585384962No Hit
AAAAAAAAAAAAAAAAAAAAAAAA33730.18150011757419415No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAA31000.16681006951675123No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATCAT27720.14916048796788206No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA22950.12349326114224002No Hit
ATAGGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACATGC19910.10713511238962958No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAATCGA251.1763987E-6163.3440744
GATCGGT252.405289E-4122.5080544
TCGAAAT356.2755917E-6116.6743444
CCCCCGG401.21855355E-5102.0900444
CTATCAT8450.093.028844
CTATCCG1400.087.5057544
CCGTATA359.168144E-487.5057544
CAATGCG203.342478E-583.324442
CGTGCGC753.5104676E-881.6720344
AACGACA2450.081.5043343
CCGTGGG555.9163E-574.2473144
GGCGATC555.9163E-574.2473144
CCTATCA6700.074.0288543
CCCCCCG356.174805E-573.6168143
GTAGACT1253.6379788E-1273.5048444
CCTTAGC24950.071.77270543
GTTCGGC1350.071.571943
CTCGTCA301.0699114E-769.5599641
CGGGTTC1054.62569E-968.0600344
GACCATA2400.068.0600344
Produced by FastQC (version 0.11.5)