##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR841045.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1858401
Sequences flagged as poor quality	0
Sequence length	24-50
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	35.316153510464105	37.0	35.0	39.0	30.0	39.0
2	36.75601659706382	38.0	37.0	39.0	33.0	39.0
3	36.788681237257194	38.0	35.0	39.0	33.0	39.0
4	37.09597067586597	38.0	36.0	39.0	33.0	40.0
5	37.628211026576075	38.0	36.0	40.0	33.0	41.0
6	37.46007078127918	38.0	36.0	40.0	33.0	41.0
7	37.50261165378193	38.0	36.0	40.0	33.0	41.0
8	37.457089185810815	38.0	36.0	40.0	33.0	41.0
9	37.48678353057279	38.0	36.0	40.0	33.0	41.0
10	37.595154651767835	39.0	36.0	40.0	33.0	41.0
11	37.5175373883247	39.0	36.0	40.0	33.0	41.0
12	37.49832678738335	39.0	36.0	40.0	33.0	41.0
13	37.32904685264375	38.0	36.0	40.0	33.0	41.0
14	37.31339038237711	38.0	36.0	40.0	33.0	41.0
15	37.208946831173684	38.0	36.0	40.0	33.0	41.0
16	37.062362213537334	38.0	35.0	40.0	32.0	41.0
17	37.185082767389815	38.0	35.0	40.0	33.0	41.0
18	37.296677089605524	38.0	36.0	40.0	33.0	41.0
19	37.35502832811648	38.0	36.0	40.0	33.0	41.0
20	37.35930942783608	38.0	36.0	40.0	33.0	41.0
21	37.295427090278146	38.0	35.0	40.0	33.0	41.0
22	37.23683855099088	38.0	35.0	40.0	33.0	41.0
23	37.155973334065145	38.0	35.0	40.0	33.0	41.0
24	37.01391895505868	38.0	35.0	40.0	32.0	41.0
25	36.928835203285715	38.0	35.0	40.0	32.0	41.0
26	36.753300098628365	38.0	35.0	40.0	32.0	41.0
27	36.65412524768137	38.0	35.0	40.0	31.0	41.0
28	36.60285944883837	38.0	35.0	40.0	31.0	40.0
29	36.510094380578316	38.0	35.0	40.0	31.0	40.0
30	36.538176339359076	38.0	35.0	40.0	31.0	40.0
31	36.5098768088435	38.0	35.0	40.0	31.0	40.0
32	36.41028995037257	38.0	35.0	40.0	31.0	40.0
33	36.3846619134796	38.0	34.0	40.0	31.0	40.0
34	36.33189130728674	38.0	34.0	40.0	31.0	40.0
35	36.19716700437596	38.0	34.0	40.0	31.0	40.0
36	36.22235086312193	38.0	34.0	40.0	31.0	40.0
37	36.179259935761955	37.0	34.0	40.0	31.0	40.0
38	36.121818290375664	37.0	34.0	40.0	31.0	41.0
39	36.01041603953442	37.0	34.0	40.0	30.0	41.0
40	35.90088703373766	37.0	34.0	39.0	30.0	41.0
41	35.74915181326321	37.0	34.0	39.0	30.0	40.0
42	35.63399401500136	36.0	34.0	39.0	30.0	40.0
43	35.50554754036409	36.0	33.0	39.0	29.0	40.0
44	35.28604304669096	36.0	33.0	39.0	29.0	40.0
45	35.02406763887652	36.0	33.0	39.0	28.0	40.0
46	34.632338030055195	36.0	32.0	39.0	27.0	40.0
47	34.489211590563734	35.0	32.0	39.0	28.0	40.0
48	35.25616075661051	36.0	33.0	39.0	29.0	40.0
49	35.764770771272204	37.0	34.0	39.0	30.0	40.0
50	35.35095614320241	36.0	33.0	39.0	29.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	3.0
20	15.0
21	50.0
22	117.0
23	259.0
24	494.0
25	783.0
26	1247.0
27	2383.0
28	4902.0
29	9969.0
30	20958.0
31	41405.0
32	72261.0
33	115599.0
34	160753.0
35	214934.0
36	304328.0
37	351170.0
38	286691.0
39	205262.0
40	64817.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	49.36830102868004	18.165293712175142	32.46640525914483
2	24.077419243747716	24.632251058840367	32.365135404038206	18.92519429337371
3	20.61847792806827	23.920940636601035	29.66065988987307	25.79992154545763
4	18.347762404346533	25.358359148536834	35.08666859305392	21.207209854062715
5	19.33920612397432	26.685683014591575	30.89112629620841	23.083984565225695
6	18.644361469887286	29.49320410395819	29.409045733401996	22.453388692752533
7	26.330915663519338	28.5940978292629	23.293950014017426	21.781036493200336
8	24.439989001297352	27.11680632974261	26.853138800506454	21.59006586845358
9	22.727979591057043	27.580000226000738	28.271347249597905	21.420672933344314
10	24.633381062537097	25.73610324144251	25.440795608698014	24.18972008732238
11	23.34915876605749	26.595013670354245	26.617721363688464	23.438106199899806
12	24.97464217894846	26.96683869627707	25.83398308545895	22.224536039315517
13	22.63139117983686	26.279581209868052	27.346950415975886	23.742077194319204
14	20.511342815678642	27.40888538049646	27.394518190638077	24.68525361318682
15	21.31838069394065	31.054761593434353	25.91432096732621	21.71253674529878
16	22.57516004349976	33.78329004342981	23.610297239400968	20.03125267366946
17	22.698707114341847	33.73572226876762	21.78254316479597	21.78302745209457
18	22.937945039848774	28.08672616943275	23.787384961587946	25.18794382913053
19	22.662331757247227	27.26962587729989	25.116054070138794	24.95198829531409
20	22.81595845030217	28.074834225767205	24.517367349673187	24.59183997425744
21	24.250202189947164	29.508324629614386	23.580540475387174	22.66093270505128
22	24.427989438232114	29.916794061131046	21.87708680742208	23.77812969321476
23	23.930034475874688	28.242989537780062	24.236211667987696	23.590764318357554
24	25.44542324288461	29.968505182681245	23.36067404182413	21.225397532610025
25	24.43224053833778	30.41789811307026	23.288441082518084	21.86142026607387
26	24.299516298379082	32.256800390342214	23.723443200323164	19.72024011095554
27	25.21826487059516	29.364714632210507	24.587530734690933	20.8294897625034
28	25.968793136783226	27.90907721568504	23.231644806949546	22.890484840582186
29	22.485662880276934	30.392984639085157	25.324315732261336	21.79703674837657
30	25.357021151704945	28.59928696676029	25.783537961841247	20.260153919693522
31	23.184101011267206	30.72659283477271	23.812797489412393	22.276508664547688
32	22.71237618945115	29.685928870305222	25.65947270491586	21.94222223532777
33	23.25545639177322	27.769255890233318	28.1242550797601	20.851032638233356
34	23.909753203250567	29.141303531461705	25.572829825905398	21.376113439382333
35	23.221473577661303	27.80230443354955	25.91580509834835	23.060416890440795
36	23.068544646929713	28.570239053703666	25.657941058522088	22.70327524084454
37	22.589206650889984	31.886815156722935	24.47506533336213	21.048912859024952
38	23.855778667572217	29.88241528190852	23.63660562909006	22.625200421429202
39	22.203366445916114	30.810254866546256	24.660219747140278	22.32615894039735
40	20.903932632271825	30.537062060715765	25.425811004534854	23.133194302477555
41	23.84537779837609	30.833604326328484	24.573922194710388	20.747095680585037
42	21.645809853225035	32.348593784410504	23.809671861438215	22.19592450092625
43	23.02745919305283	28.95874461762944	24.442638019300755	23.57115817001697
44	23.150784222924678	29.270273353870106	23.996013449574164	23.58292897363105
45	22.735057966279566	28.968855058136	27.815611082117364	20.480475893467073
46	21.440072953833106	28.98607563858518	26.209094996221577	23.364756411360137
47	21.831210021657103	31.941468755833828	24.319793856115204	21.907527366393865
48	22.853707322149123	29.52815899920223	24.653663386941993	22.96447029170665
49	21.200348874595214	28.842372276193572	23.75402564690125	26.203253202309963
50	19.000749084844458	31.41463966192246	25.896004409707007	23.68860684352607
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	68085.0
1	62399.5
2	43910.5
3	30490.5
4	28024.0
5	19911.0
6	10337.5
7	7191.5
8	7285.5
9	6745.0
10	5841.0
11	5183.0
12	4834.0
13	4520.0
14	4367.5
15	4560.0
16	5112.0
17	5773.5
18	5846.0
19	5904.0
20	6829.5
21	9066.5
22	11479.0
23	14227.0
24	17447.5
25	20137.5
26	22995.5
27	27565.0
28	32162.0
29	34888.5
30	37194.5
31	41338.0
32	47671.5
33	52935.5
34	61160.5
35	70466.0
36	75345.5
37	80243.5
38	85139.0
39	88601.5
40	92087.0
41	97592.0
42	102173.0
43	102391.0
44	104273.0
45	109775.0
46	118419.0
47	125281.0
48	132672.5
49	140540.0
50	152666.5
51	162102.0
52	155191.0
53	145905.0
54	140258.5
55	134075.5
56	131403.0
57	127395.5
58	119874.5
59	109953.0
60	93256.0
61	80612.5
62	73418.0
63	61029.0
64	45991.0
65	36584.5
66	28910.0
67	21520.5
68	15970.0
69	11856.0
70	9448.0
71	7675.0
72	5904.5
73	4217.5
74	3046.0
75	2254.5
76	1677.5
77	1116.5
78	739.0
79	655.0
80	477.5
81	354.0
82	188.5
83	73.0
84	67.0
85	59.5
86	50.0
87	25.0
88	5.0
89	3.0
90	3.0
91	3.0
92	2.5
93	2.0
94	1.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
24	16732.0
25	19678.0
26	20281.0
27	22486.0
28	23990.0
29	20953.0
30	19304.0
31	19342.0
32	17603.0
33	13707.0
34	16458.0
35	14552.0
36	12780.0
37	12673.0
38	13302.0
39	14575.0
40	17829.0
41	18296.0
42	19671.0
43	34179.0
44	75912.0
45	159606.0
46	220651.0
47	168931.0
48	301957.0
49	209188.0
50	353765.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	13.112016200514448
#Duplication Level	Percentage of deduplicated	Percentage of total
1	67.90519348434982	8.90373997065863
2	11.175285236623052	2.930610421359428
3	4.47720657430804	1.7611561540613045
4	2.3706344240736343	1.2433518789580293
5	1.4607492746249606	0.9576684076886105
6	1.0230264081267033	0.8048363302146863
7	0.7867142053926015	0.722078658439785
8	0.6810409796396834	0.7143856286599808
9	0.5275621301023078	0.6225662877011434
>10	5.684533419000984	18.048878964378208
>50	2.468411075072169	23.123058874279682
>100	1.3632535794905116	28.195683642509145
>500	0.049268779895379894	4.526477057777586
>1k	0.02486039352519169	5.3934384111330544
>5k	0.001808028620013941	1.4011370901474312
>10k+	4.5200715500348524E-4	0.6509322220332996
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGC	10983	0.5909919333878964	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTA	7324	0.39410224165828583	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAA	5768	0.3103743487008455	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAA	5527	0.2974062110384142	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAA	5022	0.270232312617137	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAA	4603	0.2476860483824535	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCT	4299	0.23132789962984307	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA	4145	0.22304120585384962	No Hit
AAAAAAAAAAAAAAAAAAAAAAAA	3373	0.18150011757419415	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3100	0.16681006951675123	No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATCAT	2772	0.14916048796788206	No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2295	0.12349326114224002	No Hit
ATAGGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACATGC	1991	0.10713511238962958	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.07097499409438544	0.0	0.0	0.0	0.0
2	0.15195859235977596	0.0	0.0	0.0	0.0
3	0.19979541552119268	0.0	0.0	0.0	0.0
4	0.2371393472130073	0.0	0.0	0.0	0.0
5	0.2685642119219695	0.0	0.0	0.0	0.0
6	0.31736960968058026	0.0	0.0	0.0	0.0
7	0.3531530600769156	0.0	0.0	0.0	0.0
8	0.3919498536645213	0.0	0.0	0.0	0.0
9	0.42665711006397433	0.0	0.0	0.0	0.0
10	0.4600191239673246	0.0	0.0	0.0	0.0
11	0.494403522167713	0.0	0.0	0.0	0.0
12	0.5292722076666985	0.0	0.0	0.0	0.0
13	0.5603742141765959	0.0	0.0	0.0	0.0
14	0.5897543102914817	0.0	0.0	0.0	0.0
15	0.6161748729149414	0.0	0.0	0.0	5.38096998441133E-5
16	0.6389901856488455	0.0	0.0	0.0	5.38096998441133E-5
17	0.6693388563609254	0.0	0.0	0.0	1.076193996882266E-4
18	0.6925308369937382	0.0	0.0	0.0	1.076193996882266E-4
19	0.714323765430604	0.0	0.0	0.0	1.076193996882266E-4
20	0.7390762273588962	0.0	0.0	0.0	1.076193996882266E-4
21	0.7615148721938915	0.0	0.0	0.0	1.076193996882266E-4
22	0.7838997073290426	0.0	0.0	0.0	1.076193996882266E-4
23	0.8099436020535934	0.0	0.0	0.0	1.076193996882266E-4
24	0.8336736796848474	0.0	0.0	0.0	1.076193996882266E-4
25	0.8586951901123601	0.0	0.0	0.0	1.076193996882266E-4
26	0.8829633647420552	0.0	0.0	0.0	1.076193996882266E-4
27	0.9081463042691001	0.0	0.0	0.0	1.076193996882266E-4
28	0.9335982922953657	0.0	0.0	0.0	1.076193996882266E-4
29	0.9663683995004307	0.0	0.0	0.0	1.076193996882266E-4
30	0.9931118203229551	0.0	0.0	0.0	1.076193996882266E-4
31	1.0216847709401793	0.0	0.0	0.0	1.076193996882266E-4
32	1.047298188065977	0.0	0.0	0.0	1.076193996882266E-4
33	1.0725887469927105	0.0	0.0	0.0	1.076193996882266E-4
34	1.0980945447188202	0.0	0.0	0.0	1.076193996882266E-4
35	1.1197798537559978	0.0	0.0	0.0	1.076193996882266E-4
36	1.1396894426983197	0.0	0.0	0.0	1.076193996882266E-4
37	1.1556709235520213	0.0	0.0	0.0	1.076193996882266E-4
38	1.1672400090185058	0.0	0.0	0.0	1.076193996882266E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAATCGA	25	1.1763987E-6	163.34407	44
GATCGGT	25	2.405289E-4	122.50805	44
TCGAAAT	35	6.2755917E-6	116.67434	44
CCCCCGG	40	1.21855355E-5	102.09004	44
CTATCAT	845	0.0	93.0288	44
CTATCCG	140	0.0	87.50575	44
CCGTATA	35	9.168144E-4	87.50575	44
CAATGCG	20	3.342478E-5	83.3244	42
CGTGCGC	75	3.5104676E-8	81.67203	44
AACGACA	245	0.0	81.50433	43
CCGTGGG	55	5.9163E-5	74.24731	44
GGCGATC	55	5.9163E-5	74.24731	44
CCTATCA	670	0.0	74.02885	43
CCCCCCG	35	6.174805E-5	73.61681	43
GTAGACT	125	3.6379788E-12	73.50484	44
CCTTAGC	2495	0.0	71.772705	43
GTTCGGC	135	0.0	71.5719	43
CTCGTCA	30	1.0699114E-7	69.55996	41
CGGGTTC	105	4.62569E-9	68.06003	44
GACCATA	240	0.0	68.06003	44
>>END_MODULE