FastQCFastQC Report
Tue 24 May 2016
ERR841044.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR841044.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences522015
Sequences flagged as poor quality0
Sequence length24-50
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAA57971.110504487418944No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAA57901.1091635297836269No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAA52060.997289349922895No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGC48950.9377125178395257No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAA44180.8463358332614964No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA42740.8187504190492612No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTA31480.603047805139699No Hit
AAAAAAAAAAAAAAAAAAAAAAAA30270.5798683945863624No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAA28990.5553480263977089No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA21140.4049692058657318No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCT20680.39615719854793446No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA15860.3038226870875358No Hit
CCTTTCCGGCGGTGACGACCTACGCACACGAGAACATGCCTCTCGCAAA14430.2764288382517744No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACACA14370.27527944599293125No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATCAT13820.26474335028686913No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA13560.25976265049854885No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCTA13530.2591879543691273No Hit
CCCTGGTGTGATTCCGTCCTGCGCGGTTGTTCTCTGGAGCAGCGTTCTT13040.24980125092190836No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA11110.21282913326245412No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10200.19539668400333327No Hit
CTCTCGGCCTTAGCGCCATTTTTTTGGAAACCTCTGCGCCATGAGAGCC10120.19386416099154238No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTC9660.185052153673745No Hit
CACTTAGGAGATTTCAACTTAACTTGACCGCTCTGACCAAAAAAAAAAAA9230.1768148424853692No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACA9040.1731751003323659No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8960.17164257732057508No Hit
CCTTTCCGGCGGTGACGACCTACGCACACGAGAACATGCCTCTCGCA8790.16838596592051952No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATC8410.161106481614513No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7970.15267760504966332No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7960.15248603967318947No Hit
CTCTTTTCCGTGGCGCCTCGGAGGCGTTCAGCTGCTTCAAGATGAAGCT7940.15210290892024175No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAA7020.134478894284647No Hit
ATCAAAAGGGACAAGCATCAAGCACGCAGCAATGCAGCTCAAAACGCTT6680.1279656714845359No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAA6680.1279656714845359No Hit
CCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCTAG6640.12719940997864046No Hit
CTCTCGGCCTTAGCGCCATTTTTTTGGAAACCTCTGCGCCATGAGAG5830.1116826144842581No Hit
CCCTGGTGTGATTCCGTCCTGCGCGGTTGTTCTCTGGAGCAGCGTTC5820.11149104910778426No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5800.11110791835483654No Hit
CCTTTCCGGCGGTGACGACCTACGCACACGAGAACATGCCTCTCG5740.10995852609599341No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAA5740.10995852609599341No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAA5730.10976696071951955No Hit
TTACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5710.10938382996657184No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTA5400.10344530329588231No Hit
CCCTGGTGTGATTCCGTCCTGCGCGGTTGTTCTCTGGAGCAGCGT5250.10057182264877447No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGTAT151.7058614E-5237.7960744
CTACCGC151.7058614E-5237.7960744
TGATAGG201.802382E-7237.7960544
TCCGCGT201.802382E-7237.7960544
AATTGTA100.0016115028237.7960544
TCGAATA100.0016115028237.7960544
GGCTATC100.0016115028237.7960544
TAGTACT100.0016115028237.7960544
TTAGAAT205.3733092E-5178.3470544
ACTCGGC351.430999E-8169.8543244
AGACTCG150.005421735158.5307244
TCTAATT150.005421735158.5307244
GCATGAT150.005421735158.5307244
CCCGCAC150.005421735158.5307244
CCAAGGG403.177047E-8148.6225444
GCCAGTC403.177047E-8148.6225444
TAACAGG251.3074503E-4142.6776344
ATAAGAA251.3074503E-4142.6776344
AGGGCTT352.927438E-6135.8834744
CCCAATC456.4177584E-8132.1089344
Produced by FastQC (version 0.11.5)