##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR841044.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	522015
Sequences flagged as poor quality	0
Sequence length	24-50
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	35.7631064241449	39.0	35.0	39.0	27.0	39.0
2	36.70648544582052	39.0	35.0	39.0	32.0	39.0
3	37.13394634253805	39.0	35.0	39.0	33.0	39.0
4	37.70643563882264	39.0	35.0	39.0	34.0	41.0
5	38.43430361196517	40.0	36.0	41.0	34.0	41.0
6	38.44564619790619	40.0	36.0	41.0	34.0	41.0
7	38.46759575874256	40.0	36.0	41.0	35.0	41.0
8	38.41735007614724	40.0	36.0	41.0	35.0	41.0
9	38.42233652289685	40.0	36.0	41.0	35.0	41.0
10	38.335676177887606	40.0	36.0	41.0	34.0	41.0
11	38.25660373744049	40.0	36.0	41.0	34.0	41.0
12	38.2235778665364	40.0	36.0	41.0	34.0	41.0
13	38.064331484727454	40.0	35.0	41.0	34.0	41.0
14	37.9627098838156	39.0	36.0	41.0	34.0	41.0
15	37.74706090821145	39.0	35.0	41.0	34.0	41.0
16	37.55529055678477	39.0	35.0	40.0	34.0	41.0
17	37.524914035037305	39.0	35.0	40.0	34.0	41.0
18	37.53035640738293	39.0	35.0	40.0	33.0	41.0
19	37.51191632424356	39.0	35.0	41.0	33.0	41.0
20	37.42471576487266	39.0	35.0	41.0	33.0	41.0
21	37.33219351934331	38.0	35.0	40.0	33.0	41.0
22	37.20966255758934	38.0	35.0	40.0	33.0	41.0
23	37.13077018859612	38.0	35.0	40.0	33.0	41.0
24	36.91694683102976	38.0	35.0	40.0	33.0	41.0
25	36.84707034089698	38.0	35.0	40.0	33.0	41.0
26	36.67183821155497	38.0	35.0	40.0	32.0	41.0
27	36.57737675350701	38.0	35.0	40.0	32.0	41.0
28	36.485909732305366	38.0	35.0	40.0	32.0	41.0
29	36.50218794842499	38.0	35.0	40.0	32.0	41.0
30	36.528095815516835	38.0	35.0	40.0	32.0	41.0
31	36.54107830690806	38.0	35.0	40.0	32.0	41.0
32	36.459490284673514	37.0	35.0	40.0	32.0	41.0
33	36.3490842093855	37.0	34.0	40.0	31.0	41.0
34	36.317891751359014	37.0	34.0	40.0	31.0	41.0
35	36.242803718153866	37.0	34.0	40.0	31.0	41.0
36	36.16503462288576	36.0	34.0	40.0	31.0	41.0
37	36.10813531006655	36.0	34.0	40.0	31.0	41.0
38	35.98273647150208	36.0	34.0	40.0	31.0	41.0
39	35.88163347610836	36.0	34.0	40.0	31.0	41.0
40	35.705863620824495	35.0	34.0	40.0	31.0	41.0
41	35.5096033894036	35.0	34.0	39.0	30.0	41.0
42	35.427262895996265	35.0	34.0	39.0	30.0	41.0
43	35.36196036558487	35.0	34.0	39.0	30.0	41.0
44	35.193001625134066	35.0	33.0	39.0	29.0	41.0
45	34.85626917865807	35.0	33.0	39.0	29.0	41.0
46	34.63262803382509	35.0	33.0	39.0	28.0	41.0
47	34.600212732516276	35.0	33.0	38.0	29.0	40.0
48	35.45682165446664	36.0	34.0	39.0	30.0	40.0
49	36.11963643074652	36.0	34.0	39.0	32.0	41.0
50	36.51460834422917	37.0	35.0	40.0	32.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	2.0
21	13.0
22	41.0
23	78.0
24	199.0
25	401.0
26	588.0
27	1114.0
28	2019.0
29	3816.0
30	6874.0
31	12759.0
32	22569.0
33	37679.0
34	53921.0
35	35542.0
36	62013.0
37	84631.0
38	70511.0
39	64632.0
40	62613.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	45.09084987979273	20.438876277501606	34.470273842705666
2	21.079279331053705	29.043035161824854	30.71559246381809	19.16209304330335
3	20.560520291562504	30.00431022097066	24.44374203806404	24.991427449402796
4	16.079231439709588	31.71364807524688	30.055266611112707	22.151853873930826
5	15.7501221229275	34.17756194745362	28.311830119824144	21.760485809794737
6	18.31824755993602	38.45215175809124	22.76811969004722	20.46148099192552
7	22.582492840244054	34.79018802141701	21.006676053370114	21.620643084968822
8	22.655670814057068	33.59596946447899	22.707585031081482	21.04077469038246
9	22.600883116385546	36.31370746051359	21.58175531354463	19.50365410955624
10	24.23455264695459	30.863289369079432	22.67827552848098	22.223882455484993
11	22.979033169544934	34.09442257406396	23.94682145149086	18.97972280490024
12	21.338467285422833	33.47394231966515	23.877666350583794	21.309924044328227
13	18.68260490598929	33.71397373638689	24.592971466337175	23.01044989128665
14	17.876114671034358	35.48422938038179	22.123119067459747	24.516536881124107
15	18.80041761252071	36.16122142084039	24.79986207292894	20.23849889370995
16	17.812898096797987	41.40359951342394	20.62201277741061	20.16148961236746
17	19.807093665890825	42.30031704069807	19.578747737133988	18.31384155627712
18	18.582416214093463	37.10333994233882	19.644454661264525	24.66978918230319
19	19.205195253009972	33.639263239562084	22.071396415811805	25.08414509161614
20	18.94083503347605	31.604264245280305	25.41823510818655	24.036665613057096
21	19.364386080859745	39.996743388599945	22.232886028179266	18.405984502361044
22	19.852686225491606	37.09261228125629	20.683888394011664	22.370813099240443
23	23.49798377441261	33.28774077373255	22.386329894734825	20.827945557120007
24	21.667959732957865	35.51947740965298	22.813712249648	19.998850607741154
25	21.257936007909354	33.25630761178261	22.705657707257025	22.780098673051015
26	22.56935421637413	39.50355531407243	21.001354405360924	16.925736064192513
27	22.61062124248497	33.673947895791585	21.301603206412825	22.413827655310623
28	21.833779831898404	32.211292664471664	23.031759340681813	22.92316816294812
29	23.613133121257253	32.57630665950927	23.236743330151498	20.573816889081982
30	24.482098410874976	32.79000999851086	24.442317101708255	18.285574488905905
31	24.13657371862111	32.06588620010252	23.132838638934366	20.664701442342004
32	21.403928161749896	30.930990096408962	24.160033624924477	23.505048116916665
33	20.509463132820052	29.125481340254755	28.571870928928632	21.79318459799656
34	22.001355672194894	33.096908264694996	24.201424347694243	20.700311715415864
35	21.947311170643125	29.12741149607149	22.597943148923928	26.32733418436146
36	21.283164692584563	31.703101675987778	22.97908582896725	24.03464780246041
37	20.844478323184926	33.54702805342292	22.748331674897983	22.86016194849417
38	24.367268239657854	29.980213128279136	22.252302148518744	23.400216483544263
39	22.166518328975915	34.01962693070776	22.199662531753535	21.614192208562795
40	19.35285219891635	32.63696689566679	22.945672178591998	25.064508726824858
41	20.971578339562924	34.662021998807155	21.905897951958103	22.460501709671824
42	20.489080823821553	35.16800863225487	22.566939716320597	21.775970827602983
43	21.173211994161615	28.9864619966106	24.72718278263766	25.11314322659013
44	22.916902147839476	31.19930875286659	21.833814342875655	24.049974756418273
45	21.924854469798802	27.517659092491336	28.964392628968945	21.593093808740914
46	19.03240961062098	31.460872960671622	27.95908530707374	21.547632121633658
47	22.939656338295876	32.05900951448007	23.200271946721838	21.801062200502226
48	22.428221164096367	29.79532371544311	24.538945348589298	23.237509771871224
49	18.181758262038784	31.201966806839003	25.182905126616483	25.43336980450573
50	18.300249613693094	34.51563057173422	25.392844407464636	21.791275407108046
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	56563.0
1	51193.0
2	29217.0
3	11857.0
4	10259.0
5	7556.0
6	4179.0
7	2571.5
8	2173.5
9	1715.5
10	1486.5
11	1311.5
12	1140.5
13	967.5
14	879.0
15	998.5
16	1109.5
17	1143.0
18	1304.5
19	1594.0
20	1909.5
21	2351.0
22	2989.0
23	3621.5
24	3913.0
25	4149.0
26	5029.0
27	6466.5
28	7594.0
29	8581.5
30	9858.0
31	12144.0
32	15691.0
33	17465.5
34	18000.5
35	19365.5
36	19480.0
37	18848.5
38	19234.5
39	19971.5
40	20532.5
41	21956.5
42	23828.5
43	23641.0
44	23611.0
45	25628.0
46	28463.5
47	30252.0
48	33012.0
49	37390.5
50	42342.5
51	44937.0
52	42201.5
53	38730.0
54	35710.5
55	32738.0
56	31093.5
57	31072.0
58	31376.5
59	28945.5
60	24660.0
61	23079.0
62	21768.0
63	16477.0
64	10935.0
65	8494.0
66	6446.5
67	4482.5
68	3793.0
69	3143.0
70	2239.0
71	1467.5
72	974.0
73	690.0
74	489.5
75	361.0
76	250.0
77	165.0
78	114.5
79	80.0
80	57.0
81	46.0
82	32.0
83	17.5
84	9.5
85	5.0
86	4.0
87	4.5
88	4.0
89	3.5
90	2.0
91	1.0
92	1.5
93	2.0
94	1.5
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
24	6170.0
25	7873.0
26	8972.0
27	10010.0
28	10231.0
29	8689.0
30	7697.0
31	5569.0
32	4681.0
33	3637.0
34	3534.0
35	3481.0
36	3307.0
37	3027.0
38	3689.0
39	4374.0
40	6233.0
41	5654.0
42	6855.0
43	10211.0
44	24659.0
45	45658.0
46	54221.0
47	48447.0
48	73418.0
49	67588.0
50	84130.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	14.970451040678908
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.02871474637867	10.783023476336886
2	10.087270307621436	3.0202197254868155
3	3.8990121308286843	1.7510991063475188
4	2.0614731023186774	1.2344472859975288
5	1.257869683165276	0.9415438253690027
6	0.9315657470440701	0.8367575644378036
7	0.7396222552080668	0.7750735132132218
8	0.5515176332087833	0.6605174180818559
9	0.49137533910016895	0.6620499410936467
>10	4.822900138199314	17.46424911161557
>50	1.9168756718022213	20.498644674961447
>100	1.155499820852741	27.435226957079777
>500	0.029431335414853866	3.0868844764997174
>1k	0.02303321902032042	7.633305556353745
>5k	0.00383886983672007	3.2169573671254654
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAA	5797	1.110504487418944	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAA	5790	1.1091635297836269	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAA	5206	0.997289349922895	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGC	4895	0.9377125178395257	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAA	4418	0.8463358332614964	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA	4274	0.8187504190492612	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTA	3148	0.603047805139699	No Hit
AAAAAAAAAAAAAAAAAAAAAAAA	3027	0.5798683945863624	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2899	0.5553480263977089	No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2114	0.4049692058657318	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCT	2068	0.39615719854793446	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1586	0.3038226870875358	No Hit
CCTTTCCGGCGGTGACGACCTACGCACACGAGAACATGCCTCTCGCAAA	1443	0.2764288382517744	No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACACA	1437	0.27527944599293125	No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATCAT	1382	0.26474335028686913	No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1356	0.25976265049854885	No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCTA	1353	0.2591879543691273	No Hit
CCCTGGTGTGATTCCGTCCTGCGCGGTTGTTCTCTGGAGCAGCGTTCTT	1304	0.24980125092190836	No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1111	0.21282913326245412	No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1020	0.19539668400333327	No Hit
CTCTCGGCCTTAGCGCCATTTTTTTGGAAACCTCTGCGCCATGAGAGCC	1012	0.19386416099154238	No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTC	966	0.185052153673745	No Hit
CACTTAGGAGATTTCAACTTAACTTGACCGCTCTGACCAAAAAAAAAAAA	923	0.1768148424853692	No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACA	904	0.1731751003323659	No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	896	0.17164257732057508	No Hit
CCTTTCCGGCGGTGACGACCTACGCACACGAGAACATGCCTCTCGCA	879	0.16838596592051952	No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATC	841	0.161106481614513	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	797	0.15267760504966332	No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	796	0.15248603967318947	No Hit
CTCTTTTCCGTGGCGCCTCGGAGGCGTTCAGCTGCTTCAAGATGAAGCT	794	0.15210290892024175	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAA	702	0.134478894284647	No Hit
ATCAAAAGGGACAAGCATCAAGCACGCAGCAATGCAGCTCAAAACGCTT	668	0.1279656714845359	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAA	668	0.1279656714845359	No Hit
CCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCTAG	664	0.12719940997864046	No Hit
CTCTCGGCCTTAGCGCCATTTTTTTGGAAACCTCTGCGCCATGAGAG	583	0.1116826144842581	No Hit
CCCTGGTGTGATTCCGTCCTGCGCGGTTGTTCTCTGGAGCAGCGTTC	582	0.11149104910778426	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	580	0.11110791835483654	No Hit
CCTTTCCGGCGGTGACGACCTACGCACACGAGAACATGCCTCTCG	574	0.10995852609599341	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAA	574	0.10995852609599341	No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAA	573	0.10976696071951955	No Hit
TTACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	571	0.10938382996657184	No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTA	540	0.10344530329588231	No Hit
CCCTGGTGTGATTCCGTCCTGCGCGGTTGTTCTCTGGAGCAGCGT	525	0.10057182264877447	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.15670047795561431	0.0	0.0	0.0	0.0
2	0.2461615087689051	0.0	0.0	0.0	0.0
3	0.32776835914676783	0.0	0.0	0.0	0.0
4	0.39098493338314033	0.0	0.0	0.0	0.0
5	0.4430907157840292	0.0	0.0	0.0	0.0
6	0.5174180818558853	0.0	0.0	0.0	0.0
7	0.5748876947980422	0.0	0.0	0.0	0.0
8	0.6331235692460945	0.0	0.0	0.0	0.0
9	0.6980642318707316	0.0	0.0	0.0	0.0
10	0.7676024635307415	0.0	0.0	0.0	0.0
11	0.8428876564849669	0.0	0.0	0.0	0.0
12	0.9091692767449211	0.0	0.0	0.0	0.0
13	0.9764087238872446	0.0	0.0	0.0	0.0
14	1.0334952060764537	0.0	0.0	0.0	0.0
15	1.083685334712604	0.0	0.0	0.0	0.0
16	1.1227646715132706	0.0	0.0	0.0	0.0
17	1.1628018351963065	0.0	0.0	0.0	0.0
18	1.1947932530674406	0.0	0.0	0.0	0.0
19	1.2216124057737805	0.0	0.0	0.0	0.0
20	1.2417267703035353	0.0	0.0	0.0	0.0
21	1.2631820924686072	0.0	0.0	0.0	0.0
22	1.2857868068925222	0.0	0.0	0.0	0.0
23	1.307433694434068	0.0	0.0	0.0	0.0
24	1.327164928210875	0.0	0.0	0.0	0.0
25	1.344597377469996	0.0	0.0	0.0	0.0
26	1.371416530176336	0.0	0.0	0.0	0.0
27	1.3894236755648783	0.0	0.0	0.0	0.0
28	1.4149018706359013	0.0	0.0	0.0	0.0
29	1.443253546354032	0.0	0.0	0.0	0.0
30	1.4731377450839536	0.0	0.0	0.0	0.0
31	1.4936352403666562	0.0	0.0	0.0	0.0
32	1.5148989971552542	0.0	0.0	0.0	0.0
33	1.5398024960968555	0.0	0.0	0.0	0.0
34	1.5666216488031952	0.0	0.0	0.0	0.0
35	1.5850119249446855	0.0	0.0	0.0	0.0
36	1.6041684625920711	0.0	0.0	0.0	0.0
37	1.6194936927099797	0.0	0.0	0.0	0.0
38	1.632328572933728	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGGTAT	15	1.7058614E-5	237.79607	44
CTACCGC	15	1.7058614E-5	237.79607	44
TGATAGG	20	1.802382E-7	237.79605	44
TCCGCGT	20	1.802382E-7	237.79605	44
AATTGTA	10	0.0016115028	237.79605	44
TCGAATA	10	0.0016115028	237.79605	44
GGCTATC	10	0.0016115028	237.79605	44
TAGTACT	10	0.0016115028	237.79605	44
TTAGAAT	20	5.3733092E-5	178.34705	44
ACTCGGC	35	1.430999E-8	169.85432	44
AGACTCG	15	0.005421735	158.53072	44
TCTAATT	15	0.005421735	158.53072	44
GCATGAT	15	0.005421735	158.53072	44
CCCGCAC	15	0.005421735	158.53072	44
CCAAGGG	40	3.177047E-8	148.62254	44
GCCAGTC	40	3.177047E-8	148.62254	44
TAACAGG	25	1.3074503E-4	142.67763	44
ATAAGAA	25	1.3074503E-4	142.67763	44
AGGGCTT	35	2.927438E-6	135.88347	44
CCCAATC	45	6.4177584E-8	132.10893	44
>>END_MODULE