FastQCFastQC Report
Tue 24 May 2016
ERR841043.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR841043.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4221195
Sequences flagged as poor quality0
Sequence length24-50
%GC27

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAA2510365.947036325021706No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAA2381865.642620158509616No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAA2275645.390985254175654No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA2005774.751663924552171No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAA1701204.030138384983399No Hit
AAAAAAAAAAAAAAAAAAAAAAAA1553053.6791714194677096No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1052142.492516929447704No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA585071.3860293116048892No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA288950.684521800106368No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAAC285690.6767988685668396No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAG202370.4794140048019578No Hit
ATCGGAAGAGCTCGTATGCCGTCTTCTGCTTG192520.4560793803650388Illumina Single End Adapter 1 (100% over 32bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAG191120.4527627839983701No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTA184360.4367483615421699No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAA181950.43103907779669026No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAA181890.4308969379524044No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAG173510.41104473970048766No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAG169810.4022794493028633No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAA156960.3718378326516543No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAA151450.3587846569514083No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA150190.3557997202214065No Hit
AAAAAAAAAAAAAAAAAAAAAAAAG145330.34428639283425666No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAA119350.282739840258505No Hit
AGTGCAGTGCTTAAGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAA118140.27987335339874136No Hit
AGTGCAGTGCTTAAGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAA114010.2700893941170688No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAG113210.26819419619325807No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA109780.26006853509491984No Hit
AAAAAAAAAAAAAAAAAAAAAAAG109600.2596421155620624No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATCAT108630.257344188079442No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCT105860.2507820652682475No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA104540.24765498869395988No Hit
AGTGCAGTGCTTAAGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA92230.21849263064132313No Hit
TCGGAAGAGCTCGTATGCCGTCTTCTGCTTG89510.21204895770036686Illumina Single End Adapter 1 (100% over 31bp)
TAAAAAAAAAAAAAAAAAAAAAAAA89500.21202526772631922No Hit
ATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA89440.2118831278820334Illumina Single End Adapter 1 (100% over 32bp)
AGTGCAGTGCTTAAGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAA85900.20349687706917116No Hit
ATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAA83560.19795342314202496Illumina Single End Adapter 1 (100% over 32bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA81440.19293114864392666No Hit
AGTGCAGTGCTTAAGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA79250.18774304432749495No Hit
ATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA74490.17646661668082142Illumina Single End Adapter 1 (100% over 32bp)
AGTGCAGTGCTTAAGGGGGAAAAAAAAAAAAAAAAAAAAAAAAA74310.176040197147964No Hit
AGTGCAGTGCTTAAGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA73920.1751162881601063No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAG71860.17023615350629384No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA71840.17018877355819859No Hit
AGTGCAGTGCTTAAGGGGGGAAAAAAAAAAAAAAAAAAAAAAAA69160.16383986051343283No Hit
ATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGGA68740.1628448816034322Illumina Single End Adapter 1 (100% over 32bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA66900.15848592637866765No Hit
ATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAA66450.15741987754652415Illumina Single End Adapter 1 (100% over 32bp)
TAAAAAAAAAAAAAAAAAAAAAAA62530.14813340771985184No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATC60480.143276963040087No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA60380.14304006329961066No Hit
ATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA59550.14107379545365709Illumina Single End Adapter 1 (100% over 32bp)
AGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTG57470.13614628085174935Illumina Single End Adapter 2 (100% over 34bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA50320.11920794940769143No Hit
AGTGCAGTGCTTAAGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA49750.11785762088697632No Hit
TCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA49300.11679157205483282Illumina Single End Adapter 1 (100% over 31bp)
ATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGA48570.11506220394935558Illumina Single End Adapter 1 (100% over 32bp)
TCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA48540.11499113402721267Illumina Single End Adapter 1 (100% over 31bp)
AGTGCAGTGCTTAAGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA48460.11480161423483161No Hit
AGTGCAGTGCTTAAGGGGGAAAAAAAAAAAAAAAAAAAAAAAA47860.1133802157919736No Hit
ATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAA45220.10712606264339838Illumina Single End Adapter 1 (100% over 32bp)
TCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAA44930.106439053396017Illumina Single End Adapter 1 (100% over 31bp)
TCGGAAGAGCTCGTATGCCGTCTTCTGCTTGGA44740.10598894388911197Illumina Single End Adapter 1 (100% over 31bp)
CGGCAAGTTTGAATTTCGTGGAGGCTCGGG44510.1054440744860164No Hit
AGTGCAGTGCTTAAGGGGGGAAAAAAAAAAAAAAAAAAAAAAA44440.10527824466768296No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACGAAA104.173811E-4373.5071744
TACGGTA400.0326.818844
TCCGGGG407.2759576E-12280.1303744
CACCGTC503.092282E-11224.1043144
ACGACAC1500.0224.1043144
GATAGTC202.7182796E-7219.2080243
TCGTAGG600.0217.879244
TATACAC353.103396E-7213.4327144
CCAGAAT5000.0212.899144
CTATCAT17700.0212.0761144
GAACACA8100.0207.5039844
ACGGTGC701.8189894E-12186.7536244
CCGAACG304.4840406E-5186.753644
CGATCTG304.4840406E-5186.753644
GACGCAT406.0371167E-7186.7535944
AACGACA2250.0180.237743
CCTTAAC36600.0176.0851343
ATTAGTG551.4446414E-8169.77644
CGTGCGC1350.0152.169644
CCAGATA501.8341925E-6149.4028844
Produced by FastQC (version 0.11.5)