FastQCFastQC Report
Tue 24 May 2016
ERR841042.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR841042.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9775804
Sequences flagged as poor quality0
Sequence length24-50
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGC1028771.052363570300714No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTA675860.6913600149921173No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATCAT503220.5147607296545634No Hit
CGGCAAGTTTGAATTTCGTGGAGGCTCGGG381140.38988097551873996No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCT376980.3856255710527748No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACACA319710.32704215428214395No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATC294310.30105963662937596No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAGAA292870.29958661200654185No Hit
ATAGGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACATGC280930.2873727828422092No Hit
TAGCTGTTAGTTGGTAGAGGGAAATTCAGGCTACCGTCGCGAAACCTG217160.22214029659350779No Hit
AGCTTTAATTTATTAATGCAAACAGTACCTAACAAACCCACAGGTCCT206980.21172683085708346No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACA201750.2063768872616513No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTA179940.18406670182830998No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAG172640.17659928533755384No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCTA171040.1749625913121826No Hit
CAGAGTGTTGCTTAACACAAAGCACCCAACTTACACTTAGGAGATTTCAA129820.13279726148355675No Hit
AGCTTTAATTTATTAATGCAAACAGTACCTAACAAACCCACAGGTC125380.12825543556315164No Hit
TAGCTGTTAGTTGGTAGAGGGAAATTCAGGCTACCGTCGCGAAACC122580.12539122101875202No Hit
TAGCTGTTAGTTGGTAGAGGGAAATTCAGGCTACCGTCGCGAAACCTGC119020.12174957681230106No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCC118810.12153476072147108No Hit
TTGAGTTCCAGCCAGGAGGACCAGGGGCTGGG116670.11934568246253709No Hit
CTGATGTTCGCCGACCGTTGACTATTCTCTACAAACCACAAAGACATTGG116520.11919224239765853No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAA115180.11782151115141015No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTC108350.11083487353060678No Hit
AACACAAAGCACCCAACTTACACTTAGGAGATTTCAACTTAACTTGACCG107350.10981193976474979No Hit
AATGCCCCAACTAAATACTACCGTATGGCCCACCATAATTACCCCCATAC106180.10861510725869708No Hit
ATAGGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTACAC100850.10316287028667923No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACC99400.10167961632618658No Hit
TAGCTGTTAGTTGGTAGAGGGAAATTCAGGCTACCGTCGCGAAA97800.10004292230081535No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATCAT112250.0106.37424544
ACATTGG30850.095.1588344
CGTGCGC7050.088.8952844
ACTTCTA45200.086.8405844
AACGACA15600.083.170243
GAACACA98750.076.82542444
CCTTAGC178700.073.78168543
TGAACAC64300.068.7551943
TCGTGCG14100.067.7355443
CCCTATC105400.064.43680642
ACACATG60000.063.07073643
TCCAGAA80550.060.32836543
CCTATCA112400.059.58640343
CCAGAAT33450.059.16561544
CATGACC31750.058.8705644
CCCATAC43700.057.6165244
ACCTTAG156650.057.32390642
TACACAT71450.056.50829342
GACATTG29450.056.29403743
AACGCTT20600.054.23558443
Produced by FastQC (version 0.11.5)