##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR841042.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	9775804
Sequences flagged as poor quality	0
Sequence length	24-50
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	37.13286364988496	39.0	37.0	39.0	34.0	39.0
2	37.77602711756496	39.0	37.0	39.0	35.0	39.0
3	38.00512090872526	39.0	38.0	39.0	35.0	39.0
4	38.46029983825371	39.0	38.0	40.0	35.0	41.0
5	39.09968100833446	40.0	38.0	41.0	35.0	41.0
6	39.05830047329099	40.0	38.0	41.0	35.0	41.0
7	39.07983905978475	40.0	38.0	41.0	35.0	41.0
8	39.02678470231195	40.0	38.0	41.0	35.0	41.0
9	39.08141079751599	40.0	38.0	41.0	35.0	41.0
10	39.072477721525516	40.0	38.0	41.0	35.0	41.0
11	38.96535701820536	40.0	38.0	41.0	35.0	41.0
12	38.906489021261066	40.0	38.0	41.0	35.0	41.0
13	38.86232252610629	40.0	38.0	41.0	35.0	41.0
14	38.79873778156763	40.0	38.0	41.0	35.0	41.0
15	38.73559780863037	40.0	38.0	41.0	35.0	41.0
16	38.69792438555437	40.0	38.0	41.0	35.0	41.0
17	38.68682637254184	40.0	38.0	41.0	35.0	41.0
18	38.66486275706837	40.0	38.0	41.0	35.0	41.0
19	38.6793165042998	40.0	38.0	41.0	35.0	41.0
20	38.58373439156513	40.0	38.0	41.0	35.0	41.0
21	38.49873534698527	40.0	38.0	41.0	35.0	41.0
22	38.43493629782267	40.0	38.0	41.0	35.0	41.0
23	38.31402399229772	40.0	37.0	41.0	34.0	41.0
24	38.157594812661955	40.0	37.0	41.0	34.0	41.0
25	38.08057546057418	40.0	37.0	41.0	34.0	41.0
26	37.933005365718586	39.0	36.0	41.0	33.0	41.0
27	37.820845130908	39.0	36.0	41.0	33.0	41.0
28	37.69894055372846	39.0	36.0	41.0	33.0	41.0
29	37.63838152413895	39.0	36.0	41.0	33.0	41.0
30	37.62174947430987	39.0	36.0	41.0	33.0	41.0
31	37.54966931852499	39.0	36.0	40.0	33.0	41.0
32	37.50960502874683	39.0	36.0	40.0	33.0	41.0
33	37.34609827397064	38.0	35.0	40.0	32.0	41.0
34	37.29026396700028	38.0	35.0	40.0	32.0	41.0
35	37.16895754623459	38.0	35.0	40.0	32.0	41.0
36	37.153790067256075	38.0	35.0	40.0	32.0	41.0
37	37.07122447693632	38.0	35.0	40.0	32.0	41.0
38	36.88592596753784	38.0	35.0	40.0	31.0	41.0
39	36.8259463247274	38.0	35.0	40.0	31.0	41.0
40	36.61837262422326	38.0	34.0	40.0	31.0	41.0
41	36.51230967509685	38.0	34.0	40.0	31.0	41.0
42	36.35840374443778	37.0	34.0	40.0	31.0	41.0
43	36.208440795241295	37.0	34.0	40.0	30.0	41.0
44	35.949459530403196	37.0	34.0	40.0	29.0	41.0
45	35.755827342630845	37.0	34.0	40.0	29.0	41.0
46	35.460158151109425	36.0	33.0	40.0	28.0	41.0
47	35.600281078812046	36.0	34.0	40.0	29.0	41.0
48	36.48428103023055	37.0	35.0	40.0	31.0	41.0
49	37.176002231148324	38.0	35.0	40.0	32.0	41.0
50	37.04981407887309	38.0	35.0	40.0	32.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	7.0
20	28.0
21	66.0
22	202.0
23	412.0
24	799.0
25	1569.0
26	2782.0
27	5922.0
28	12647.0
29	27189.0
30	57617.0
31	114828.0
32	210138.0
33	356032.0
34	545776.0
35	617535.0
36	1028610.0
37	1658352.0
38	1618016.0
39	1621040.0
40	1896237.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	50.527639465766704	18.026343408685364	31.446017125547936
2	25.674062205011477	23.726150810715925	31.18301062500844	19.41677635926416
3	25.988757548739727	23.1171369638753	24.819472649001558	26.074632838383422
4	20.604474066787752	25.952770738856874	29.348092494489457	24.094662699865914
5	18.78500223613321	26.65897352279158	28.738260300636142	25.81776394043907
6	23.069519397074657	26.50700648253586	27.080197188896175	23.343276931493307
7	25.6625746588209	24.784907717053247	24.837281925865128	24.715235698260727
8	21.81284526571932	24.079779013572693	29.533253735447236	24.574121985260753
9	23.04672843277136	27.031853339121774	26.869258017038806	23.052160211068063
10	27.158451621984238	22.283098147221445	26.809764189216562	23.748686041577756
11	23.75681836501632	23.91284645232249	26.49449600257943	25.83583918008176
12	21.98568015479852	25.71818133833289	26.530278225709107	25.76586028115948
13	24.328853156221218	24.766484679930162	25.101454570897697	25.803207592950923
14	20.836168564754367	25.929591059722558	26.076484348499623	27.157756027023456
15	21.006016487237265	29.4081284772076	27.112194557092185	22.473660478462946
16	22.474775476267734	31.01396059086291	22.922022577375735	23.589241355493627
17	23.62061473409246	31.805926141727063	21.353609380875476	23.219849743305
18	22.693581008784548	27.04946825856983	23.315616802464532	26.941333930181088
19	24.469997557234166	23.89643859471814	25.077170123296256	26.556393724751437
20	24.79676351939953	24.530452942796316	26.095521145882223	24.577262391921934
21	22.966244004073733	29.121052345157494	24.023210776320802	23.889492874447974
22	25.4251005850772	26.94482213432266	22.230795543773176	25.399281736826968
23	27.257123813038802	25.30907943735369	25.091061563836593	22.34273518577091
24	23.375693702533315	28.62009099200434	23.86640525935258	24.137810046109763
25	23.108690808893417	25.94568158337608	25.69380551844944	25.251822089281063
26	24.2997914508815	30.255746315299763	26.05327020666487	19.391192027153867
27	25.143163653004088	28.28175242974694	22.838077878491294	23.737006038757677
28	25.18795454628676	25.93096362897358	23.39743646748714	25.48364535725252
29	25.572120302458366	27.999316164241435	24.927708264885716	21.500855268414483
30	26.461294691072286	27.808962920119345	25.832065229302113	19.897677159506262
31	24.137457593346003	29.298158238699777	23.173439924183292	23.39094424377092
32	22.608475093759324	27.830112709281714	26.12736196202774	23.434050234931224
33	22.17614042678819	25.75170318496755	29.460241910800423	22.611914477443833
34	22.603779106658006	28.294197103784068	26.293900522683273	22.808123266874652
35	22.05975184199496	26.024632682371056	25.061209541376904	26.854405934257073
36	23.046755611677455	26.52695274180965	25.487840965110102	24.938450681402788
37	21.06259605304701	30.774182141192934	24.118456821151323	24.04476498460873
38	24.07240089398298	27.394421424437354	23.260857985530293	25.272319696049365
39	22.127643572645564	29.20830487210772	26.468697755801163	22.19535379944555
40	20.78347268378192	27.666963722117284	27.043226732765763	24.506336861335036
41	21.685663375705236	29.79092971549706	24.978136208842656	23.545270699955047
42	21.281567186365567	30.93241804480084	24.687934643279792	23.098080125553796
43	21.885002895965265	26.167385974507795	25.26451315593146	26.683097973595483
44	21.00665674977874	27.599948145640152	23.536679625088684	27.856715479492422
45	20.255146655525397	26.26157962802897	28.990635380289138	24.492638336156496
46	20.33592839737063	28.295537750226714	26.5788900315121	24.78964382089056
47	21.990605519991604	29.353661454507296	25.27600377794102	23.37972924756008
48	22.442305601492357	27.67083108871993	25.349648777608746	24.537214532178968
49	19.996574123688358	28.409574025673667	23.619500162172574	27.974351688465404
50	19.501695179851964	30.241114870635776	26.206733885610145	24.050456063902114
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	117131.0
1	104862.5
2	100627.0
3	104538.0
4	91592.5
5	60686.5
6	29639.0
7	21367.5
8	21620.5
9	20386.0
10	18377.0
11	16032.0
12	14184.5
13	12923.0
14	12849.0
15	13514.5
16	14076.5
17	15353.0
18	17179.5
19	19583.0
20	22582.5
21	26732.5
22	31546.0
23	38445.5
24	45561.0
25	51709.0
26	61499.5
27	77276.5
28	93665.5
29	107284.0
30	124329.5
31	158127.0
32	211296.0
33	253648.0
34	332504.0
35	429997.0
36	438843.0
37	423292.5
38	431671.0
39	445485.5
40	485005.0
41	544828.0
42	593827.0
43	628084.0
44	664725.0
45	694847.0
46	734437.5
47	748322.5
48	785942.5
49	887527.0
50	1006972.5
51	1056811.5
52	970966.0
53	847622.0
54	744875.0
55	682859.0
56	639413.0
57	582972.0
58	514956.0
59	464912.5
60	435013.5
61	391651.0
62	344905.5
63	289674.0
64	238937.0
65	206681.0
66	167159.5
67	123816.5
68	89296.0
69	67587.5
70	54516.0
71	43591.5
72	35432.5
73	26559.0
74	17493.0
75	11501.5
76	8487.0
77	5576.0
78	3620.5
79	2571.5
80	1833.0
81	1305.5
82	901.0
83	681.5
84	575.5
85	481.5
86	375.5
87	335.5
88	298.5
89	257.0
90	226.0
91	117.5
92	36.5
93	35.5
94	19.0
95	2.0
96	1.0
97	1.0
98	0.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
24	49195.0
25	51670.0
26	53258.0
27	63386.0
28	58938.0
29	54154.0
30	100561.0
31	55966.0
32	71973.0
33	52262.0
34	55567.0
35	55565.0
36	55026.0
37	62095.0
38	54221.0
39	72164.0
40	76185.0
41	75656.0
42	99503.0
43	173436.0
44	457739.0
45	850002.0
46	1121657.0
47	1062429.0
48	1658987.0
49	1466255.0
50	1767954.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	9.868664985429195
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.13221204900866	6.921113053987474
2	12.874491100846194	2.541080790642812
3	4.512197233790884	1.3358808854538773
4	2.1980656573069015	0.8676789435175611
5	1.1532254608838313	0.5690397863064856
6	0.8021303656305552	0.4749573511828668
7	0.5764419832677177	0.3982098971483841
8	0.43444938737987554	0.34299483657415536
9	0.37127019592008154	0.32975470643489513
>10	3.2407221463895506	7.229438391742447
>50	1.5320133973406016	11.326959350066854
>100	2.0163590227977926	34.155772372166865
>500	0.08772874042519714	5.9517297683675325
>1k	0.056609866463051745	11.432891458136565
>5k	0.00761419256520579	5.194233918720932
>10k+	0.004469199983925138	10.928264489550289
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGC	102877	1.052363570300714	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTA	67586	0.6913600149921173	No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATCAT	50322	0.5147607296545634	No Hit
CGGCAAGTTTGAATTTCGTGGAGGCTCGGG	38114	0.38988097551873996	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCT	37698	0.3856255710527748	No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACACA	31971	0.32704215428214395	No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATC	29431	0.30105963662937596	No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAGAA	29287	0.29958661200654185	No Hit
ATAGGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACATGC	28093	0.2873727828422092	No Hit
TAGCTGTTAGTTGGTAGAGGGAAATTCAGGCTACCGTCGCGAAACCTG	21716	0.22214029659350779	No Hit
AGCTTTAATTTATTAATGCAAACAGTACCTAACAAACCCACAGGTCCT	20698	0.21172683085708346	No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACA	20175	0.2063768872616513	No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTA	17994	0.18406670182830998	No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAG	17264	0.17659928533755384	No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCTA	17104	0.1749625913121826	No Hit
CAGAGTGTTGCTTAACACAAAGCACCCAACTTACACTTAGGAGATTTCAA	12982	0.13279726148355675	No Hit
AGCTTTAATTTATTAATGCAAACAGTACCTAACAAACCCACAGGTC	12538	0.12825543556315164	No Hit
TAGCTGTTAGTTGGTAGAGGGAAATTCAGGCTACCGTCGCGAAACC	12258	0.12539122101875202	No Hit
TAGCTGTTAGTTGGTAGAGGGAAATTCAGGCTACCGTCGCGAAACCTGC	11902	0.12174957681230106	No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCC	11881	0.12153476072147108	No Hit
TTGAGTTCCAGCCAGGAGGACCAGGGGCTGGG	11667	0.11934568246253709	No Hit
CTGATGTTCGCCGACCGTTGACTATTCTCTACAAACCACAAAGACATTGG	11652	0.11919224239765853	No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAA	11518	0.11782151115141015	No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTC	10835	0.11083487353060678	No Hit
AACACAAAGCACCCAACTTACACTTAGGAGATTTCAACTTAACTTGACCG	10735	0.10981193976474979	No Hit
AATGCCCCAACTAAATACTACCGTATGGCCCACCATAATTACCCCCATAC	10618	0.10861510725869708	No Hit
ATAGGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTACAC	10085	0.10316287028667923	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACC	9940	0.10167961632618658	No Hit
TAGCTGTTAGTTGGTAGAGGGAAATTCAGGCTACCGTCGCGAAA	9780	0.10004292230081535	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.06230689567835034	0.0	0.0	0.0	0.0
2	0.11308532781549221	0.0	0.0	0.0	0.0
3	0.13813697574133033	0.0	0.0	0.0	0.0
4	0.1636898612124384	0.0	0.0	0.0	0.0
5	0.1898974242936949	0.0	0.0	0.0	0.0
6	0.22547506067020165	0.0	0.0	0.0	0.0
7	0.24970836158335416	0.0	0.0	0.0	0.0
8	0.27713321584598055	0.0	0.0	0.0	0.0
9	0.30092665523981454	0.0	0.0	0.0	0.0
10	0.3230527125953016	0.0	0.0	0.0	0.0
11	0.34615055702835285	0.0	0.0	0.0	0.0
12	0.3723581201096094	0.0	0.0	0.0	0.0
13	0.3966630263863719	0.0	0.0	0.0	0.0
14	0.4202825670400102	0.0	0.0	0.0	0.0
15	0.4460195805889725	0.0	0.0	0.0	0.0
16	0.4694652225024151	0.0	0.0	0.0	0.0
17	0.5004396569325653	0.0	0.0	0.0	0.0
18	0.5296239572724658	0.0	0.0	0.0	0.0
19	0.5560463364445523	0.0	0.0	0.0	0.0
20	0.5822845875387845	0.0	0.0	0.0	0.0
21	0.6066406405038399	0.0	0.0	0.0	0.0
22	0.6274471133013714	0.0	0.0	0.0	0.0
23	0.6527135773180395	0.0	0.0	0.0	0.0
24	0.6765070167118735	0.0	0.0	0.0	0.0
25	0.7016609580142973	0.0	0.0	0.0	0.0
26	0.7340470410413302	0.0	0.0	0.0	0.0
27	0.7570221334224786	0.0	0.0	0.0	0.0
28	0.7842117129189579	0.0	0.0	0.0	0.0
29	0.8122707861164156	0.0	0.0	0.0	0.0
30	0.839409218924602	0.0	0.0	0.0	0.0
31	0.8641846747336588	0.0	0.0	0.0	0.0
32	0.8926426920998007	0.0	0.0	0.0	0.0
33	0.9189934659082772	0.0	0.0	0.0	0.0
34	0.9432881428473812	0.0	0.0	0.0	0.0
35	0.967275939656728	0.0	0.0	0.0	0.0
36	0.9872538361039154	0.0	0.0	0.0	0.0
37	1.004582334097533	0.0	0.0	0.0	1.0229337658570078E-5
38	1.0168063925995243	0.0	0.0	0.0	1.0229337658570078E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTATCAT	11225	0.0	106.374245	44
ACATTGG	3085	0.0	95.15883	44
CGTGCGC	705	0.0	88.89528	44
ACTTCTA	4520	0.0	86.84058	44
AACGACA	1560	0.0	83.1702	43
GAACACA	9875	0.0	76.825424	44
CCTTAGC	17870	0.0	73.781685	43
TGAACAC	6430	0.0	68.75519	43
TCGTGCG	1410	0.0	67.73554	43
CCCTATC	10540	0.0	64.436806	42
ACACATG	6000	0.0	63.070736	43
TCCAGAA	8055	0.0	60.328365	43
CCTATCA	11240	0.0	59.586403	43
CCAGAAT	3345	0.0	59.165615	44
CATGACC	3175	0.0	58.87056	44
CCCATAC	4370	0.0	57.61652	44
ACCTTAG	15665	0.0	57.323906	42
TACACAT	7145	0.0	56.508293	42
GACATTG	2945	0.0	56.294037	43
AACGCTT	2060	0.0	54.235584	43
>>END_MODULE