FastQCFastQC Report
Tue 24 May 2016
ERR841041.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR841041.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1589339
Sequences flagged as poor quality0
Sequence length24-50
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGC407582.5644623330831244No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATCAT304571.9163312546914157No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTA257841.6223096519999824No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATC177291.1154951838468696No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCT154940.9748706852345536No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTA112430.7074010012967655No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCTA88390.5561431513352406No Hit
AGCTTTAATTTATTAATGCAAACAGTACCTAACAAACCCACAGGTCCT69800.43917628649394497No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACACA67290.42338355756701374No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTC57460.3615339458730957No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAGAA57350.3608418342468158No Hit
CGGCAAGTTTGAATTTCGTGGAGGCTCGGG52160.32818674933415715No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACC45540.28653421327986034No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACA43120.27130775750170355No Hit
AGCTTTAATTTATTAATGCAAACAGTACCTAACAAACCCACAGGTC42370.26658881459524997No Hit
CGCAATAGATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAG39240.24689509286565042No Hit
AACACAAAGCACCCAACTTACACTTAGGAGATTTCAACTTAACTTGACCG34850.21927354705320892No Hit
AGGTTTTCTGTTTCCTGTGATACCTTGGGCTGGAATCTGAAGCAATGAA34390.21637926207058408No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACT31950.2010269678149218No Hit
AGCTTTAATTTATTAATGCAAACAGTACCTAACAAACCCACAGG31520.1983214405485551No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCT30540.1921553551507891No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAG30120.1895127471231751No Hit
ACAACCTTAGCCAAACCATTTACCCAAATAAAGTATAGGCGATAGAAAT30070.18919815092941153No Hit
CACTTAGGAGATTTCAACTTAACTTGACCGCTCTGACCAAAAAAAAAAAA29910.1881914431093681No Hit
AATGCCCCAACTAAATACTACCGTATGGCCCACCATAATTACCCCCATAC29450.18529715812674324No Hit
ATAGGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACATGC28140.1770547378501377No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA27760.17466380677753457No Hit
CCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCTAG27360.172147037227426No Hit
TAGCTGTTAGTTGGTAGAGGGAAATTCAGGCTACCGTCGCGAAACCTG25910.16302374760828242No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTT25620.16119908968445373No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAA24940.15692058144926915No Hit
CGCAATAGATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCA22560.1419458026261232No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCC22310.14037282165730533No Hit
AACACAAAGCACCCAACTTACACTTAGGAGATTTCAACTTAACTTGAC22270.14012114470229448No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGCC22110.13911443688225106No Hit
AGGTTTTCTGTTTCCTGTGATACCTTGGGCTGGAATCTGAAGCAATG19900.12520928511790122No Hit
ATAGGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACAT19420.12218916165777094No Hit
CACTTAGGAGATTTCAACTTAACTTGACCGCTCTGACCAAAAAAAAAA18440.11602307626000495No Hit
ACAACCTTAGCCAAACCATTTACCCAAATAAAGTATAGGCGATAGAA18370.11558264158873595No Hit
TCAGTTCTTTGACCATTCGTTGGAGCTCCGGTTTTCAGCCTCTTTCCG17600.11073786020477695No Hit
CCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCT17380.10935363695221724No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA17310.10891320228094824No Hit
TAGCTGTTAGTTGGTAGAGGGAAATTCAGGCTACCGTCGCGAAACCTGC16760.10545264414954895No Hit
AGGTTTTCTGTTTCCTGTGATACCTTGGGCTGGAATCTGAAGCAATGAAG16630.10463469404576367No Hit
TTGAGTTCCAGCCAGGAGGACCAGGGGCTGGG16510.10387966318073111No Hit
AATGCCCCAACTAAATACTACCGTATGGCCCACCATAATTACCCCCAT16480.10369090546447296No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGATTC100.0018840465225.7966244
ACGATAG100.0018840465225.7966244
TGCTCTA100.0018840465225.7966244
GTCTATC152.1025604E-5225.796644
GGCTCGA302.910383E-11225.796644
CTATCAT45300.0173.2104344
GACGACT206.621654E-5169.3474644
GGTAGTA404.3550244E-8141.1228844
AATCGCA1850.0140.3600644
ACTTCTA17300.0133.7812344
GAACACA12250.0122.5753144
AACGTTC152.568636E-4120.5106343
TATCGAA152.568636E-4120.5106343
GCGCAAA501.6487684E-7112.89831544
CCCCCAG603.7016434E-9112.898344
TCGTCAC1250.0108.3823844
CCCATAC8200.0106.0142744
ATCGTCA700.0103.2948343
TCGTGCG2700.0102.6572143
CAGTTAT451.3264329E-5100.3540644
Produced by FastQC (version 0.11.5)