##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR841041.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1589339
Sequences flagged as poor quality	0
Sequence length	24-50
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.61930022481044	39.0	37.0	39.0	32.0	39.0
2	37.41858911157406	39.0	37.0	39.0	35.0	39.0
3	37.67611755578891	39.0	37.0	39.0	35.0	39.0
4	38.154284894537916	39.0	38.0	40.0	35.0	41.0
5	38.71197271318454	40.0	38.0	41.0	35.0	41.0
6	38.70204342811697	40.0	38.0	41.0	35.0	41.0
7	38.72295526630882	40.0	38.0	41.0	35.0	41.0
8	38.651609883102346	40.0	38.0	41.0	35.0	41.0
9	38.6496147140415	40.0	38.0	41.0	35.0	41.0
10	38.61982560045403	40.0	38.0	41.0	35.0	41.0
11	38.44090782394442	40.0	38.0	41.0	34.0	41.0
12	38.41094505325799	40.0	38.0	41.0	34.0	41.0
13	38.238614921045794	40.0	37.0	41.0	34.0	41.0
14	38.16636601757083	39.0	37.0	41.0	34.0	41.0
15	38.04535910840922	39.0	37.0	40.0	34.0	41.0
16	38.091823707843325	39.0	37.0	41.0	34.0	41.0
17	38.035818035044755	39.0	36.0	40.0	34.0	41.0
18	37.99040167012827	39.0	36.0	40.0	34.0	41.0
19	38.01820442334832	39.0	36.0	41.0	34.0	41.0
20	37.82953353563966	39.0	36.0	41.0	34.0	41.0
21	37.754857837126	39.0	36.0	40.0	34.0	41.0
22	37.64479761712259	39.0	35.0	40.0	33.0	41.0
23	37.512545781611095	39.0	35.0	40.0	33.0	41.0
24	37.232761544264626	38.0	35.0	40.0	33.0	41.0
25	37.160901913437044	38.0	35.0	40.0	33.0	41.0
26	36.98112769909553	38.0	35.0	40.0	32.0	41.0
27	36.86739818562171	38.0	35.0	40.0	32.0	41.0
28	36.61942744994142	37.0	35.0	40.0	32.0	41.0
29	36.60469319214918	37.0	35.0	40.0	32.0	41.0
30	36.483256327777234	37.0	35.0	40.0	32.0	41.0
31	36.41912497910295	37.0	34.0	40.0	32.0	41.0
32	36.37117943926342	37.0	34.0	40.0	32.0	41.0
33	36.17381942647908	37.0	34.0	39.0	31.0	41.0
34	35.93584836461841	36.0	34.0	39.0	31.0	41.0
35	35.73285572870698	36.0	34.0	39.0	31.0	41.0
36	35.708773565116985	36.0	34.0	39.0	31.0	41.0
37	35.54427729886227	35.0	34.0	39.0	31.0	41.0
38	35.27717438081225	35.0	34.0	38.0	30.0	40.0
39	35.20551728405247	35.0	34.0	38.0	31.0	40.0
40	34.98317399962031	35.0	33.0	38.0	30.0	40.0
41	34.88011372025182	35.0	33.0	38.0	30.0	40.0
42	34.77359759379631	35.0	33.0	37.0	30.0	40.0
43	34.580550594929456	35.0	33.0	37.0	29.0	40.0
44	34.16278382360903	35.0	32.0	37.0	29.0	40.0
45	34.007309984806575	35.0	32.0	37.0	29.0	39.0
46	33.80372518688126	35.0	32.0	37.0	28.0	39.0
47	33.88686753008787	35.0	32.0	36.0	29.0	39.0
48	34.5979769952706	35.0	34.0	36.0	30.0	39.0
49	35.140227391626794	35.0	34.0	37.0	31.0	39.0
50	35.52011607390233	36.0	34.0	38.0	31.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	5.0
21	26.0
22	47.0
23	126.0
24	244.0
25	465.0
26	980.0
27	2026.0
28	4320.0
29	8980.0
30	17125.0
31	31798.0
32	55502.0
33	93419.0
34	137532.0
35	167757.0
36	232054.0
37	274131.0
38	244860.0
39	214240.0
40	103702.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	46.25331663037275	17.37376355830946	36.372919811317786
2	23.61786881212882	23.530033554830027	33.55294244965989	19.299155183381266
3	26.497430692885533	23.708724192887733	20.26288916335659	29.53095595087014
4	21.77660020926939	28.515061921968822	26.562426266517086	23.145911602244706
5	14.521131111738905	29.165080577523106	27.38194935127119	28.9318389594668
6	19.456138684069288	30.04752290102993	24.276129887959712	26.220208526941075
7	21.510640587061665	25.176630032988555	21.35585925972999	31.956870120219786
8	19.196848501169352	26.85411985737467	29.349371027829807	24.599660613626167
9	18.529212458764306	31.50385160120025	29.12103711039621	20.84589882963924
10	32.32752735571203	20.94279445731842	25.654627489792926	21.075050697176625
11	19.93130477512979	22.795766038585853	25.598503528825507	31.674425657458855
12	17.28152395429798	28.01686738952483	25.95726902819348	28.744339627983713
13	22.82722565796221	25.1498264372799	25.195568723853125	26.827379180904764
14	16.63012107549113	26.423060152679824	22.753169713950264	34.19364905787878
15	20.284847977681288	35.41893831334914	23.698971710881064	20.597241998088514
16	18.07682313213229	38.03927293044467	20.5517513884703	23.33215254895274
17	19.04672319750538	39.723243436422315	18.790516057304327	22.439517308767982
18	18.275396249635854	31.135522377541857	20.21368631865197	30.37539505417032
19	24.098634715438305	22.823639261353303	23.186934945911478	29.890791077296914
20	19.5503287844821	23.868413220842125	28.35562457096944	28.22563342370633
21	18.316042077870108	38.38381868185453	21.415129182635045	21.885010057640315
22	24.734936976944503	28.305855453116042	19.198295643660668	27.760911926278787
23	28.086393148346577	23.550671065140918	26.51221671399242	21.850719072520086
24	19.532774316870093	28.41294399747316	25.874970663904932	26.17931102175181
25	20.96613715810034	24.62859599107442	23.53887873979911	30.866388111026126
26	20.28457787699642	33.80701550365429	27.629447601690103	18.278959017659183
27	22.1483092596166	31.45706154548022	20.07432331408373	26.32030588081945
28	20.9966959856676	25.526500921497185	20.874001175503736	32.602801917331476
29	22.23715162511364	31.34846993736979	25.576804449680512	20.83757398783606
30	23.181921564804885	31.297137472788123	27.285765344250578	18.235175618156415
31	23.37715977146004	32.58339971219437	20.706402200172423	23.33303831617317
32	20.20537542229201	31.088467334514576	23.768607370399923	24.93754987279349
33	19.008311609055873	23.91799831512474	30.257241978600756	26.816448097218633
34	22.22211849431534	32.865668656019736	23.786150101690396	21.12606274797453
35	18.64797471852088	26.928973637405	21.241445637040158	33.181606007033956
36	19.08249232622465	27.026257767357976	27.05472037511289	26.836529531304485
37	15.87353917203242	38.21454058028822	21.20300048027221	24.708919767407153
38	24.536488666838803	25.62567711385896	21.94579810291994	27.892036116382297
39	17.543313257982014	32.744960536809394	28.26856969996975	21.443156505238843
40	15.912885868653962	28.148925172971424	29.370152608393806	26.568036349980805
41	18.50284195669926	32.12100305012662	22.21365440714957	27.162500586024557
42	17.93852256946161	34.72981774052501	21.41100281561998	25.9206568743934
43	18.341363706907217	24.40730550630689	22.882309314685035	34.369021472100854
44	18.15907730713457	26.16876722110768	21.18691934778187	34.485236123975874
45	16.078538314698665	24.76551482332775	34.944797226226186	24.2111496357474
46	16.64228640702712	31.697987270876656	28.732325771657113	22.92740055043911
47	18.636051305542832	31.96851705326282	27.915816432765585	21.47961520842877
48	22.624321243707463	27.042107641418667	21.36314826144461	28.970422853429255
49	15.339448978692335	31.760090910658818	21.56636918849206	31.334090922156786
50	16.576621899470666	29.53358997914753	32.29435525030258	21.595432871079225
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	24854.0
1	19915.5
2	14494.0
3	12962.0
4	10191.0
5	6668.0
6	3552.5
7	2163.0
8	2048.0
9	2030.5
10	1874.5
11	1588.5
12	1438.0
13	1296.5
14	1285.0
15	1485.5
16	1596.5
17	1608.0
18	1707.5
19	2100.0
20	2734.5
21	3413.5
22	4065.5
23	5319.0
24	6831.5
25	8093.0
26	9716.5
27	12622.0
28	15827.0
29	17516.5
30	20002.0
31	29382.5
32	45100.5
33	54997.0
34	71225.0
35	87971.0
36	78277.5
37	65367.0
38	64334.0
39	68790.0
40	78257.5
41	86701.0
42	93962.0
43	98567.0
44	100729.0
45	107758.5
46	117884.0
47	120014.5
48	131964.0
49	166376.0
50	208909.5
51	226894.5
52	191982.5
53	137795.0
54	104220.0
55	92441.0
56	88085.5
57	81318.5
58	70066.0
59	61185.5
60	57253.0
61	53205.5
62	46536.5
63	36872.5
64	29895.0
65	26068.5
66	20394.5
67	13857.5
68	9553.0
69	7503.5
70	6193.5
71	4844.0
72	3475.0
73	2229.5
74	1360.0
75	892.5
76	639.5
77	454.5
78	297.5
79	188.5
80	132.5
81	102.0
82	72.0
83	92.0
84	101.0
85	79.0
86	76.5
87	50.0
88	25.0
89	25.0
90	22.0
91	10.0
92	1.0
93	1.0
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
24	7369.0
25	8341.0
26	8575.0
27	9976.0
28	8540.0
29	7688.0
30	13515.0
31	7724.0
32	10081.0
33	7880.0
34	8598.0
35	8405.0
36	8483.0
37	10230.0
38	9414.0
39	11218.0
40	14181.0
41	13447.0
42	17441.0
43	31451.0
44	83332.0
45	141816.0
46	181114.0
47	172682.0
48	266007.0
49	247523.0
50	274308.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	10.497549236623666
#Duplication Level	Percentage of deduplicated	Percentage of total
1	67.24477829508072	7.059053710584523
2	11.736282690063241	2.464044107877459
3	4.524180779311274	1.4247843145861956
4	2.407512408668212	1.0109192019110795
5	1.5387045669631858	0.8076313476156871
6	1.052841083692834	0.6631350668643445
7	0.7937050418291708	0.5832370429170717
8	0.6177862599781536	0.5188193345464205
9	0.4958869572564569	0.4685037974639236
>10	5.458989577486335	13.564145142503298
>50	2.386456482791492	18.017743747850336
>100	1.6275521609061225	28.573535765313895
>500	0.06882220566117318	4.870126635261505
>1k	0.03906125186174694	8.212468673002217
>5k	0.0037201192249282805	2.5543350457846676
>10k+	0.0037201192249282805	9.20751706591739
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGC	40758	2.5644623330831244	No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATCAT	30457	1.9163312546914157	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTA	25784	1.6223096519999824	No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATC	17729	1.1154951838468696	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCT	15494	0.9748706852345536	No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTA	11243	0.7074010012967655	No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCTA	8839	0.5561431513352406	No Hit
AGCTTTAATTTATTAATGCAAACAGTACCTAACAAACCCACAGGTCCT	6980	0.43917628649394497	No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACACA	6729	0.42338355756701374	No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTC	5746	0.3615339458730957	No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAGAA	5735	0.3608418342468158	No Hit
CGGCAAGTTTGAATTTCGTGGAGGCTCGGG	5216	0.32818674933415715	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACC	4554	0.28653421327986034	No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACA	4312	0.27130775750170355	No Hit
AGCTTTAATTTATTAATGCAAACAGTACCTAACAAACCCACAGGTC	4237	0.26658881459524997	No Hit
CGCAATAGATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAG	3924	0.24689509286565042	No Hit
AACACAAAGCACCCAACTTACACTTAGGAGATTTCAACTTAACTTGACCG	3485	0.21927354705320892	No Hit
AGGTTTTCTGTTTCCTGTGATACCTTGGGCTGGAATCTGAAGCAATGAA	3439	0.21637926207058408	No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACT	3195	0.2010269678149218	No Hit
AGCTTTAATTTATTAATGCAAACAGTACCTAACAAACCCACAGG	3152	0.1983214405485551	No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCT	3054	0.1921553551507891	No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAG	3012	0.1895127471231751	No Hit
ACAACCTTAGCCAAACCATTTACCCAAATAAAGTATAGGCGATAGAAAT	3007	0.18919815092941153	No Hit
CACTTAGGAGATTTCAACTTAACTTGACCGCTCTGACCAAAAAAAAAAAA	2991	0.1881914431093681	No Hit
AATGCCCCAACTAAATACTACCGTATGGCCCACCATAATTACCCCCATAC	2945	0.18529715812674324	No Hit
ATAGGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACATGC	2814	0.1770547378501377	No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2776	0.17466380677753457	No Hit
CCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCTAG	2736	0.172147037227426	No Hit
TAGCTGTTAGTTGGTAGAGGGAAATTCAGGCTACCGTCGCGAAACCTG	2591	0.16302374760828242	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTT	2562	0.16119908968445373	No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAA	2494	0.15692058144926915	No Hit
CGCAATAGATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCA	2256	0.1419458026261232	No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCC	2231	0.14037282165730533	No Hit
AACACAAAGCACCCAACTTACACTTAGGAGATTTCAACTTAACTTGAC	2227	0.14012114470229448	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGCC	2211	0.13911443688225106	No Hit
AGGTTTTCTGTTTCCTGTGATACCTTGGGCTGGAATCTGAAGCAATG	1990	0.12520928511790122	No Hit
ATAGGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACAT	1942	0.12218916165777094	No Hit
CACTTAGGAGATTTCAACTTAACTTGACCGCTCTGACCAAAAAAAAAA	1844	0.11602307626000495	No Hit
ACAACCTTAGCCAAACCATTTACCCAAATAAAGTATAGGCGATAGAA	1837	0.11558264158873595	No Hit
TCAGTTCTTTGACCATTCGTTGGAGCTCCGGTTTTCAGCCTCTTTCCG	1760	0.11073786020477695	No Hit
CCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCT	1738	0.10935363695221724	No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1731	0.10891320228094824	No Hit
TAGCTGTTAGTTGGTAGAGGGAAATTCAGGCTACCGTCGCGAAACCTGC	1676	0.10545264414954895	No Hit
AGGTTTTCTGTTTCCTGTGATACCTTGGGCTGGAATCTGAAGCAATGAAG	1663	0.10463469404576367	No Hit
TTGAGTTCCAGCCAGGAGGACCAGGGGCTGGG	1651	0.10387966318073111	No Hit
AATGCCCCAACTAAATACTACCGTATGGCCCACCATAATTACCCCCAT	1648	0.10369090546447296	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.021770056608439104	0.0	0.0	0.0	0.0
2	0.039764958891715364	0.0	0.0	0.0	0.0
3	0.05587228401241019	0.0	0.0	0.0	0.0
4	0.07298631695314844	0.0	0.0	0.0	0.0
5	0.0905407845651557	0.0	0.0	0.0	0.0
6	0.11954655363015694	0.0	0.0	0.0	0.0
7	0.13911443688225106	0.0	0.0	0.0	0.0
8	0.16661014421718714	0.0	0.0	0.0	0.0
9	0.1859263505142704	0.0	0.0	0.0	0.0
10	0.20385833355879393	0.0	0.0	0.0	0.0
11	0.21977690096323063	0.0	0.0	0.0	0.0
12	0.24645465819438145	0.0	0.0	0.0	0.0
13	0.2640091258063887	0.0	0.0	0.0	0.0
14	0.28288489743220296	0.0	0.0	0.0	0.0
15	0.3092480584695902	0.0	0.0	0.0	0.0
16	0.33517078483570845	0.0	0.0	0.0	0.0
17	0.36423947313946237	0.0	0.0	0.0	0.0
18	0.40985592123518017	0.0	0.0	0.0	0.0
19	0.4296125622035324	0.0	0.0	0.0	0.0
20	0.44842541459059393	0.0	0.0	0.0	0.0
21	0.4708246635865602	0.0	0.0	0.0	0.0
22	0.4890083235860946	0.0	0.0	0.0	0.0
23	0.5110929763882973	0.0	0.0	0.0	0.0
24	0.5302833442078752	0.0	0.0	0.0	0.0
25	0.5531230278751104	0.0	0.0	0.0	0.0
26	0.5780390464211852	0.0	0.0	0.0	0.0
27	0.6012562455209367	0.0	0.0	0.0	0.0
28	0.6263610217832697	0.0	0.0	0.0	0.0
29	0.6514657980456026	0.0	0.0	0.0	0.0
30	0.673613370086558	0.0	0.0	0.0	0.0
31	0.6976485192900949	0.0	0.0	0.0	0.0
32	0.7201106875248138	0.0	0.0	0.0	0.0
33	0.7435166443408234	0.0	0.0	0.0	0.0
34	0.7664192472468114	0.0	0.0	0.0	0.0
35	0.7848545842013567	0.0	0.0	0.0	0.0
36	0.8005214746507825	0.0	0.0	0.0	0.0
37	0.8173838306365099	0.0	0.0	0.0	0.0
38	0.8278284242694605	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATGATTC	10	0.0018840465	225.79662	44
ACGATAG	10	0.0018840465	225.79662	44
TGCTCTA	10	0.0018840465	225.79662	44
GTCTATC	15	2.1025604E-5	225.7966	44
GGCTCGA	30	2.910383E-11	225.7966	44
CTATCAT	4530	0.0	173.21043	44
GACGACT	20	6.621654E-5	169.34746	44
GGTAGTA	40	4.3550244E-8	141.12288	44
AATCGCA	185	0.0	140.36006	44
ACTTCTA	1730	0.0	133.78123	44
GAACACA	1225	0.0	122.57531	44
AACGTTC	15	2.568636E-4	120.51063	43
TATCGAA	15	2.568636E-4	120.51063	43
GCGCAAA	50	1.6487684E-7	112.898315	44
CCCCCAG	60	3.7016434E-9	112.8983	44
TCGTCAC	125	0.0	108.38238	44
CCCATAC	820	0.0	106.01427	44
ATCGTCA	70	0.0	103.29483	43
TCGTGCG	270	0.0	102.65721	43
CAGTTAT	45	1.3264329E-5	100.35406	44
>>END_MODULE