FastQCFastQC Report
Tue 24 May 2016
ERR841040.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR841040.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences83413428
Sequences flagged as poor quality0
Sequence length24-50
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGCAAGTTTGAATTTCGTGGAGGCTCGGG3273600.3924547975656869No Hit
CACTTAGGAGATTTCAACTTAACTTGACCGCTCTGACCAAAAAAAAAAAA2092220.25082532275259084No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGC2032230.24363343513468838No Hit
CAGAGTGTTGCTTAACACAAAGCACCCAACTTACACTTAGGAGATTTCAA1969860.2361562217536486No Hit
CAAAGCACCCAACTTACACTTAGGAGATTTCAACTTAACTTGACCGCTCT1802010.2160335623660018No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAGAA1750710.20988347343787384No Hit
AACACAAAGCACCCAACTTACACTTAGGAGATTTCAACTTAACTTGACCG1656990.19864787237853357No Hit
AAACAGCCGGGGCTCCAGCGGGAGAACGATAATGCAAAGTGCTATGTTCT1358720.1628898407100593No Hit
CACTTAGGAGATTTCAACTTAACTTGACCGCTCTGACCAAAAAAAAAA1191820.14288107185811857No Hit
AGGTTTTCTGTTTCCTGTGATACCTTGGGCTGGAATCTGAAGCAATGAA1124570.13481882077787283No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACACA1077230.12914347555647754No Hit
CAAAGCACCCAACTTACACTTAGGAGATTTCAACTTAACTTGACCGCT1068270.12806930797760765No Hit
ATAGGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACATGC1037800.12441641890080335No Hit
AAACAGCCGGGGCTCCAGCGGGAGAACGATAATGCAAAGTGCTATGTTC1010570.121151956493144No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAG993950.11915947154215985No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA933900.11196039083779172No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAGAAT920060.11030118555971588No Hit
AGGTTTTCTGTTTCCTGTGATACCTTGGGCTGGAATCTGAAGCAATGAAG911200.10923900645828871No Hit
CACAAAGCACCCAACTTACACTTAGGAGATTTCAACTTAACTTGACCGCT903620.10833027986812867No Hit
AAAACAGCCGGGGCTCCAGCGGGAGAACGATAATGCAAAGTGCTATGTT892820.10703552430431226No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATCAT865980.1038178169586796No Hit
TAGCTGTTAGTTGGTAGAGGGAAATTCAGGCTACCGTCGCGAAACCTG842960.1010580694513598No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCT838590.10053417298711186No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGCGC98450.0109.9056244
CTATACG82450.089.87675544
ATGTTCT383750.084.2975744
CCAGAAT331000.067.3988444
AACGACA118050.063.80154843
ACGACAC75650.063.1923944
CTATCAT336900.062.81167244
TATGTTC505100.058.2891643
TCCGGGC133350.058.04895443
CCCGTTT89950.055.58520543
CAATGAA458200.055.193843
TCCAGAA702300.053.10953543
GCAATGA419550.052.9087542
TCGTGCG278250.050.35926443
CCCTATC237800.049.38860342
TCGCAAA73350.048.3724843
CCTTAGC533000.048.36321343
GAACACA538750.046.43499844
ACCTTAG396300.046.17741442
AACCTTA526900.044.61325541
Produced by FastQC (version 0.11.5)