##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR841040.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	83413428
Sequences flagged as poor quality	0
Sequence length	24-50
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	37.30737136231831	39.0	37.0	39.0	34.0	39.0
2	37.8815814163638	39.0	38.0	39.0	35.0	39.0
3	38.11627197481921	39.0	38.0	39.0	35.0	39.0
4	38.61758573211977	39.0	38.0	40.0	35.0	41.0
5	39.245519630244665	40.0	39.0	41.0	35.0	41.0
6	39.203548546164534	40.0	38.0	41.0	35.0	41.0
7	39.23694948731756	40.0	39.0	41.0	35.0	41.0
8	39.20141344628589	40.0	38.0	41.0	35.0	41.0
9	39.25924746792567	40.0	39.0	41.0	35.0	41.0
10	39.23276799030487	40.0	39.0	41.0	35.0	41.0
11	39.154416336899615	40.0	39.0	41.0	35.0	41.0
12	39.09082697092847	40.0	38.0	41.0	35.0	41.0
13	39.0235772830245	40.0	38.0	41.0	35.0	41.0
14	39.001134409678016	40.0	38.0	41.0	35.0	41.0
15	38.982900546899955	40.0	38.0	41.0	35.0	41.0
16	38.930477836254376	40.0	38.0	41.0	35.0	41.0
17	38.91445386946572	40.0	38.0	41.0	35.0	41.0
18	38.93094471551991	40.0	38.0	41.0	35.0	41.0
19	38.903992040705965	40.0	38.0	41.0	35.0	41.0
20	38.81522687210505	40.0	38.0	41.0	35.0	41.0
21	38.717876407141546	40.0	38.0	41.0	35.0	41.0
22	38.66925445145355	40.0	38.0	41.0	35.0	41.0
23	38.55044444402884	40.0	38.0	41.0	35.0	41.0
24	38.4148086324902	40.0	38.0	41.0	34.0	41.0
25	38.307940889197354	40.0	37.0	41.0	34.0	41.0
26	38.17041040554683	40.0	37.0	41.0	34.0	41.0
27	38.06405496065545	40.0	37.0	41.0	34.0	41.0
28	37.974442431792646	39.0	36.0	41.0	33.0	41.0
29	37.92026452111844	39.0	36.0	41.0	33.0	41.0
30	37.87396769037302	39.0	36.0	41.0	33.0	41.0
31	37.78519976643966	39.0	36.0	41.0	33.0	41.0
32	37.71368916742031	39.0	36.0	41.0	33.0	41.0
33	37.619874401826806	39.0	36.0	41.0	33.0	41.0
34	37.516036286502	39.0	35.0	41.0	33.0	41.0
35	37.43920445422776	39.0	35.0	41.0	33.0	41.0
36	37.41494255061069	38.0	35.0	41.0	33.0	41.0
37	37.32854925341299	38.0	35.0	41.0	33.0	41.0
38	37.21189875656363	38.0	35.0	40.0	32.0	41.0
39	37.10737618054665	38.0	35.0	40.0	32.0	41.0
40	36.87866120070779	38.0	35.0	40.0	31.0	41.0
41	36.75957138829603	38.0	35.0	40.0	31.0	41.0
42	36.62464674689235	38.0	34.0	40.0	31.0	41.0
43	36.53024157084439	38.0	34.0	40.0	31.0	41.0
44	36.29527117723395	37.0	34.0	40.0	30.0	41.0
45	36.06416713381782	37.0	34.0	40.0	30.0	41.0
46	35.74769690025546	37.0	34.0	40.0	29.0	41.0
47	35.85990929883317	37.0	34.0	40.0	30.0	41.0
48	36.62662675621274	38.0	35.0	40.0	31.0	41.0
49	37.189632647147214	38.0	35.0	40.0	33.0	41.0
50	37.0420414675253	38.0	35.0	40.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	7.0
19	38.0
20	168.0
21	536.0
22	1358.0
23	2835.0
24	5958.0
25	12351.0
26	22920.0
27	47890.0
28	103381.0
29	216180.0
30	439791.0
31	857466.0
32	1558458.0
33	2698959.0
34	4288449.0
35	4459966.0
36	7511546.0
37	1.3184654E7
38	1.4384372E7
39	1.422699E7
40	1.9389155E7
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	51.32097316513595	19.87375701667602	28.80526981818803
2	27.052722254742967	26.792936743949667	27.334031877936965	18.8203091233704
3	25.470542944236747	21.98825949222468	26.23568713660827	26.3055104269303
4	20.398401561916383	25.053149715894662	30.5743351058537	23.97411361633525
5	20.625400984599267	25.63293885967617	28.68045418298838	25.06120597273619
6	23.79391241419787	27.05781615880839	27.612145373044733	21.536126053949012
7	27.957805546608157	25.049874463857307	24.56637197550495	22.425948014029586
8	23.056840440606276	23.74926612535334	27.288446891308677	25.905446542731703
9	24.67155168350113	25.351186861664527	26.68126048002727	23.296000974807075
10	26.803955353567293	22.02618144407157	27.24836941121758	23.921493791143554
11	25.64386036262651	24.529627292142937	26.686256078577657	23.140256266652894
12	24.388719523671895	24.944740312075414	28.157702618336224	22.508837545916467
13	23.35658714325948	24.033131691938138	27.548235998645204	25.062045166157183
14	20.17495432509979	27.61806528320596	27.627989344833065	24.578991046861184
15	20.908313467227362	27.82340752138852	27.139763396368267	24.128515615015846
16	21.977302023842014	30.888785676090425	23.797602467554743	23.336309832512818
17	22.610882267061367	30.663363937039012	22.31730363605246	24.408450159847163
18	23.820046096175307	27.3198495091222	23.995266085935228	24.864838308767265
19	23.999157545713143	25.40932498302312	26.299536568620578	24.291980902643157
20	24.56066426139446	25.528473664935582	26.735897965972576	23.174964107697384
21	25.169698097049793	27.17026687837359	24.759810854434612	22.900224170142007
22	26.330517192028125	28.790703818095093	23.231178078426414	21.647600911450372
23	26.732450079860044	26.525238838044157	23.438335372093807	23.303975710001993
24	25.3852497226226	27.68566111441913	24.6976781723921	22.23141099056617
25	23.956506404013243	27.266287900656234	26.676796431035694	22.10040926429483
26	25.052530016270435	30.091703520133134	24.673441585716677	20.182324877879754
27	25.833699555179656	29.24219723770901	23.038934897678097	21.885168309433233
28	25.64322459082236	27.54612891026621	24.137527184975944	22.673119313935487
29	25.85378587968068	28.95037504017044	23.71058987160924	21.48524920853964
30	26.81094386298589	28.079372009469484	24.882537461710836	20.227146665833786
31	23.15686974215169	28.53113185562274	25.89489617971073	22.417102222514842
32	22.806247322385264	28.9864323178554	27.563881470320595	20.64343888943874
33	23.18480033202797	27.975887039486754	26.928262563381335	21.911050065103943
34	22.37824477377088	27.58712497820095	27.271542339294825	22.763087908733347
35	22.89479873436246	27.60800394277393	26.554214347335993	22.942982975527613
36	22.724316683950025	28.42605252959488	25.440918294278397	23.408712492176694
37	21.350356268884514	30.20937226264086	25.78662348186026	22.653647986614367
38	22.24135671351888	29.871264028178953	24.961598953980378	22.925780304321794
39	22.971946476185245	30.01317893272534	25.333470179980395	21.681404411109025
40	21.747149967269245	29.374347256289408	26.994065688845875	21.884437087595472
41	22.228246847769594	30.080122304981945	26.11557513164462	21.576055715603843
42	22.32541765584113	31.183354564346534	24.832112048588183	21.65911573122415
43	21.18641941660633	28.675302819449875	26.840901802433358	23.29737596151043
44	20.304997916368492	30.030068946411298	25.42389956004834	24.24103357717187
45	20.137308269906168	28.877239481688427	27.669943659566748	23.315508588838654
46	21.384965570310218	29.188219771051994	27.316777198898357	22.11003745973943
47	22.682948283757934	29.220742290980397	26.285270796842635	21.811038628419034
48	21.184351449815185	30.068152833256477	25.655124462411543	23.092371254516795
49	19.242260431358915	31.117612048738195	24.328643483981846	25.31148403592104
50	19.640917583812328	32.592973584821316	25.732135549348122	22.03397328201823
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	771197.0
1	673994.0
2	577107.0
3	533768.5
4	429095.5
5	299136.5
6	186566.5
7	144454.5
8	147089.5
9	148293.0
10	145334.5
11	141451.5
12	140982.0
13	144848.5
14	158461.5
15	177035.0
16	196441.0
17	225664.5
18	263755.0
19	310386.5
20	356247.0
21	422021.5
22	501685.0
23	623713.5
24	766463.5
25	886698.0
26	1058670.0
27	1241579.0
28	1358985.5
29	1487593.0
30	1673402.0
31	1882775.5
32	2191548.0
33	2573737.5
34	3076857.5
35	3445164.5
36	3594461.5
37	3802932.5
38	3946026.0
39	4236955.5
40	4672303.5
41	5135057.5
42	5561478.0
43	5587268.5
44	5529106.0
45	5536895.0
46	5624945.0
47	5814437.5
48	6103963.0
49	6489576.0
50	6944604.5
51	7353629.5
52	7441129.5
53	7213511.0
54	6693680.0
55	6208413.5
56	5755745.0
57	5282970.0
58	4711814.0
59	4111606.5
60	3747797.5
61	3353604.0
62	2850631.0
63	2330472.5
64	1868498.5
65	1538706.5
66	1164090.0
67	811996.0
68	578145.0
69	420015.0
70	323486.0
71	233727.0
72	170209.5
73	117367.5
74	72317.0
75	46820.5
76	35116.0
77	26699.0
78	18578.5
79	12923.5
80	9626.5
81	6789.0
82	4893.5
83	4326.0
84	3813.0
85	3152.5
86	2631.0
87	1409.0
88	367.0
89	347.5
90	297.0
91	212.0
92	171.0
93	155.0
94	89.0
95	27.0
96	30.0
97	26.0
98	11.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
24	409852.0
25	372724.0
26	376520.0
27	429724.0
28	412129.0
29	344505.0
30	751255.0
31	376167.0
32	395446.0
33	384541.0
34	373395.0
35	389992.0
36	385173.0
37	376750.0
38	380896.0
39	574187.0
40	614587.0
41	540064.0
42	749941.0
43	1398882.0
44	4016564.0
45	7912296.0
46	9858204.0
47	9204749.0
48	1.3536055E7
49	1.3815958E7
50	1.5032872E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	7.2485952218008745
#Duplication Level	Percentage of deduplicated	Percentage of total
1	65.55761703959237	4.752006296258409
2	12.140852256255812	1.7600824730657247
3	4.123430459637745	0.8966723498147502
4	1.938857834178998	0.562159825303243
5	1.2426475146145446	0.45037244184088593
6	0.9143747189610678	0.3976759331278028
7	0.6177486016607354	0.31344666935905247
8	0.4751188041230359	0.2755155114683187
9	0.3671998913949894	0.23955150403903677
>10	5.99871351410171	11.38900095787445
>50	4.063286448832171	21.41024618749468
>100	2.3913654889385794	28.08953307880068
>500	0.09795193163449084	4.831538738470017
>1k	0.05892649183485986	8.305774980644152
>5k	0.00657851382401017	3.2961902571120425
>10k+	0.005330490414766901	13.03023279532658
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGGCAAGTTTGAATTTCGTGGAGGCTCGGG	327360	0.3924547975656869	No Hit
CACTTAGGAGATTTCAACTTAACTTGACCGCTCTGACCAAAAAAAAAAAA	209222	0.25082532275259084	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGC	203223	0.24363343513468838	No Hit
CAGAGTGTTGCTTAACACAAAGCACCCAACTTACACTTAGGAGATTTCAA	196986	0.2361562217536486	No Hit
CAAAGCACCCAACTTACACTTAGGAGATTTCAACTTAACTTGACCGCTCT	180201	0.2160335623660018	No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAGAA	175071	0.20988347343787384	No Hit
AACACAAAGCACCCAACTTACACTTAGGAGATTTCAACTTAACTTGACCG	165699	0.19864787237853357	No Hit
AAACAGCCGGGGCTCCAGCGGGAGAACGATAATGCAAAGTGCTATGTTCT	135872	0.1628898407100593	No Hit
CACTTAGGAGATTTCAACTTAACTTGACCGCTCTGACCAAAAAAAAAA	119182	0.14288107185811857	No Hit
AGGTTTTCTGTTTCCTGTGATACCTTGGGCTGGAATCTGAAGCAATGAA	112457	0.13481882077787283	No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACACA	107723	0.12914347555647754	No Hit
CAAAGCACCCAACTTACACTTAGGAGATTTCAACTTAACTTGACCGCT	106827	0.12806930797760765	No Hit
ATAGGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACATGC	103780	0.12441641890080335	No Hit
AAACAGCCGGGGCTCCAGCGGGAGAACGATAATGCAAAGTGCTATGTTC	101057	0.121151956493144	No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAG	99395	0.11915947154215985	No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	93390	0.11196039083779172	No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAGAAT	92006	0.11030118555971588	No Hit
AGGTTTTCTGTTTCCTGTGATACCTTGGGCTGGAATCTGAAGCAATGAAG	91120	0.10923900645828871	No Hit
CACAAAGCACCCAACTTACACTTAGGAGATTTCAACTTAACTTGACCGCT	90362	0.10833027986812867	No Hit
AAAACAGCCGGGGCTCCAGCGGGAGAACGATAATGCAAAGTGCTATGTT	89282	0.10703552430431226	No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATCAT	86598	0.1038178169586796	No Hit
TAGCTGTTAGTTGGTAGAGGGAAATTCAGGCTACCGTCGCGAAACCTG	84296	0.1010580694513598	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCT	83859	0.10053417298711186	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.044169147442303894	0.0	0.0	0.0	1.198847744274459E-6
2	0.08020890833068268	0.0	0.0	0.0	1.198847744274459E-6
3	0.09769889807190277	0.0	0.0	0.0	1.198847744274459E-6
4	0.11676177605361093	2.397695488548918E-6	0.0	0.0	1.198847744274459E-6
5	0.13799936384343298	2.397695488548918E-6	0.0	0.0	1.198847744274459E-6
6	0.16952066758364132	2.397695488548918E-6	0.0	0.0	1.198847744274459E-6
7	0.1933573572830504	2.397695488548918E-6	0.0	0.0	1.198847744274459E-6
8	0.21699623710465418	2.397695488548918E-6	0.0	0.0	1.198847744274459E-6
9	0.23956214819513233	2.397695488548918E-6	0.0	0.0	1.198847744274459E-6
10	0.25901465169372967	2.397695488548918E-6	0.0	0.0	1.198847744274459E-6
11	0.27994053907004035	2.397695488548918E-6	0.0	3.716428007250823E-5	1.198847744274459E-6
12	0.3005547260328397	2.397695488548918E-6	0.0	3.716428007250823E-5	1.198847744274459E-6
13	0.3221459739072227	2.397695488548918E-6	0.0	3.716428007250823E-5	1.198847744274459E-6
14	0.3445836082890635	2.397695488548918E-6	0.0	3.716428007250823E-5	1.198847744274459E-6
15	0.36754753683064073	2.397695488548918E-6	0.0	3.716428007250823E-5	3.596543232823377E-6
16	0.3904191540959089	2.397695488548918E-6	0.0	3.716428007250823E-5	3.596543232823377E-6
17	0.41886541337205324	3.596543232823377E-6	0.0	3.716428007250823E-5	4.795390977097836E-6
18	0.4412407076711917	3.596543232823377E-6	0.0	3.716428007250823E-5	4.795390977097836E-6
19	0.4710260798776907	3.596543232823377E-6	0.0	3.716428007250823E-5	4.795390977097836E-6
20	0.4950366025000195	3.596543232823377E-6	0.0	3.716428007250823E-5	4.795390977097836E-6
21	0.5194391483347262	3.596543232823377E-6	0.0	3.716428007250823E-5	4.795390977097836E-6
22	0.5424857973706584	3.596543232823377E-6	0.0	3.716428007250823E-5	4.795390977097836E-6
23	0.5667900376903344	3.596543232823377E-6	0.0	3.716428007250823E-5	4.795390977097836E-6
24	0.5920581515964073	3.596543232823377E-6	0.0	3.716428007250823E-5	5.994238721372295E-6
25	0.6187265196677926	3.596543232823377E-6	0.0	3.716428007250823E-5	5.994238721372295E-6
26	0.6535542454867099	3.596543232823377E-6	0.0	3.716428007250823E-5	7.193086465646754E-6
27	0.6791436505882482	3.596543232823377E-6	0.0	3.716428007250823E-5	7.193086465646754E-6
28	0.706447407964099	3.596543232823377E-6	0.0	3.716428007250823E-5	7.193086465646754E-6
29	0.7378871900576967	3.596543232823377E-6	0.0	3.716428007250823E-5	7.193086465646754E-6
30	0.7664689191289441	3.596543232823377E-6	0.0	3.716428007250823E-5	7.193086465646754E-6
31	0.7964449081267827	3.596543232823377E-6	0.0	3.716428007250823E-5	7.193086465646754E-6
32	0.8258454502073694	3.596543232823377E-6	0.0	3.716428007250823E-5	7.193086465646754E-6
33	0.8545350755756016	3.596543232823377E-6	0.0	3.716428007250823E-5	7.193086465646754E-6
34	0.8805320889101932	3.596543232823377E-6	0.0	3.716428007250823E-5	7.193086465646754E-6
35	0.9053050786978807	3.596543232823377E-6	0.0	3.716428007250823E-5	7.193086465646754E-6
36	0.926033156196386	3.596543232823377E-6	0.0	3.716428007250823E-5	7.193086465646754E-6
37	0.9446308812533157	3.596543232823377E-6	0.0	3.716428007250823E-5	7.193086465646754E-6
38	0.9583708752504453	3.596543232823377E-6	0.0	3.716428007250823E-5	7.193086465646754E-6
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTGCGC	9845	0.0	109.90562	44
CTATACG	8245	0.0	89.876755	44
ATGTTCT	38375	0.0	84.29757	44
CCAGAAT	33100	0.0	67.39884	44
AACGACA	11805	0.0	63.801548	43
ACGACAC	7565	0.0	63.19239	44
CTATCAT	33690	0.0	62.811672	44
TATGTTC	50510	0.0	58.28916	43
TCCGGGC	13335	0.0	58.048954	43
CCCGTTT	8995	0.0	55.585205	43
CAATGAA	45820	0.0	55.1938	43
TCCAGAA	70230	0.0	53.109535	43
GCAATGA	41955	0.0	52.90875	42
TCGTGCG	27825	0.0	50.359264	43
CCCTATC	23780	0.0	49.388603	42
TCGCAAA	7335	0.0	48.37248	43
CCTTAGC	53300	0.0	48.363213	43
GAACACA	53875	0.0	46.434998	44
ACCTTAG	39630	0.0	46.177414	42
AACCTTA	52690	0.0	44.613255	41
>>END_MODULE