FastQCFastQC Report
Tue 24 May 2016
ERR841039.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR841039.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences886849
Sequences flagged as poor quality0
Sequence length24-50
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGC111841.2610940532153727No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTA72280.8150203698713084No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATCAT71230.8031806993073228No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCT43650.4921920191599698No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATC43470.49016236134900076No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAA37870.42701745167441135No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAA35820.40390190438282053No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAA32230.3634215069307176No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAA30290.3415463060791634No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTA27550.310650403845525No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA26820.30241901383437314No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA26140.29475141765960156No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGCC22560.25438377897477477No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAA19910.22450270564662078No Hit
AAAAAAAAAAAAAAAAAAAAAAAA19790.22314960043930818No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCTA18880.21288855261718734No Hit
ATAGGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACATGC16430.1852626546345545No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACACA15710.1771440233906787No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAG14970.1687998746122508No Hit
CCCGAAGCGTTTACTTTGAAAAAATTAGAGTGTTCAAAGCAGGCCCGAGC14560.16417676515393262No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA14360.16192158980841156No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTT12970.14624812115704025No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA12670.1428653581387587No Hit
CCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCTAG12550.14151225293144606No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA12380.13959535388775315No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTC12250.13812948991316448No Hit
CTTTTTCGCAACGGGTTTGCCGCCAGAACACAGGTGTCGTGAAAACTAC11810.13316810415301816No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACC11810.13316810415301816No Hit
CCTTTCCGGCGGTGACGACCTACGCACACGAGAACATGCCTCTCGCAAA10740.12110291605448052No Hit
CTCTTTTTCCGGCTGGAACCATGGAGGG10490.1182839468725792No Hit
AAGATAAAATTTGAAATCTGGTTAGGCTGGTGTTAGGGTTCTTTGTTTTT10470.1180584293380271No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACA10110.11399911371608921No Hit
CTTTTCGATCCGCCATCTGCGGTGGAGCCGCCACCAAAATGCAGATTTT9890.11151842083601604No Hit
CTCTCGGCCTTAGCGCCATTTTTTTGGAAACCTCTGCGCCATGAGAGCC9720.10960152179232316No Hit
ATAGGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACAT9690.10926324549049499No Hit
TTACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA9410.10610600000676552No Hit
CTCTTTTTCCGGCTGGAACCATGGAGGGTGTAGAAGAGAAGAAGAAGGA9390.1058804824722134No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAA9090.10249771945393185No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA9090.10249771945393185No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATGCG100.0030285192.662744
CTGTCCG205.1623465E-7192.662744
ATTAGTC100.0030285192.662744
CGTGCAC100.0030285192.662744
CCAGGTA100.0030285192.662744
CGATCAG153.9571205E-5192.662744
TGGTCAT205.1623465E-7192.662744
GACTAGC205.1623465E-7192.662744
TGGGGTA302.0128937E-8160.5522644
TTTAACG201.245472E-4144.4970244
CTATCAT10950.0124.9228544
GATTATA251.409353E-7115.9119443
GTTACGC206.4818796E-6115.91193443
AATCGCA608.731149E-11112.3865844
CTATTGG358.363979E-6110.0929744
CGATACC358.363979E-6110.0929744
AATACAT358.363979E-6110.0929744
ATCTACC556.0808816E-9105.08874544
TTGGCGG359.002179E-999.3530943
GTACCAA401.6236729E-596.3313544
Produced by FastQC (version 0.11.5)