FastQCFastQC Report
Tue 24 May 2016
ERR841038.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR841038.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2054885
Sequences flagged as poor quality0
Sequence length24-50
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGC340821.658584300337975No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTA214091.0418587901512737No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCT145810.7095774216075352No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATCAT105170.5118047968621116No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATC59140.2878019937855403No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAA57630.28045365069091455No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAA55140.26833618426335293No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAA54820.26677891950157795No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA44320.2156811695058361No Hit
TCAGTTCTTTGACCATTCGTTGGAGCTCCGGTTTTCAGCCTCTTTCCG41630.2025904126021651No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAA41310.2010331478403901No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGCC39970.19451210165045732No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTA38930.1894509911746886No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA37360.18181066093723006No Hit
AAAAAAAAAAAAAAAAAAAAAAAA36090.17563026641393556No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACC35960.1749976276044645No Hit
AAGATAAAATTTGAAATCTGGTTAGGCTGGTGTTAGGGTTCTTTGTTTTT31600.15377989522528024No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCTA29670.14438764213082483No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAA28240.13742861522664285No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTT25840.12574912951333042No Hit
ATAGGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACATGC25430.12375388403730622No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAG25080.12205062570411482No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTTTTT23640.11504293427612737No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTTTT23560.11465361808568363No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA22760.11076045618124615No Hit
CCTTTCCGGCGGTGACGACCTACGCACACGAGAACATGCCTCTCGCAAA22640.11017648189558053No Hit
TCAGTTCTTTGACCATTCGTTGGAGCTCCGGTTTTCAGCCTCTTTC22040.10725661046725242No Hit
AAAGATAAAATTTGAAATCTGGTTAGGCTGGTGTTAGGGTTCTTTGTTTT21250.10341211308662042No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21130.10282813880095479No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGACAA100.0034231339184.9647244
ATCGACT201.4665237E-4138.7235444
GACTAAG401.4330726E-7115.6029544
ACCGATT153.1219862E-4114.7494243
ATCCCGT206.8214013E-6114.7494143
CGAGTGC504.1763997E-9110.9788444
GATGCGT253.5649465E-4110.9788444
CTATCAT20600.0106.3996244
ACGAACA1500.0104.8133544
TACGCTT209.798558E-486.0620643
CCCGAGA1400.085.8764844
ACGATTG150.00142445778.3259542
CGCTATA204.5429144E-578.3259442
CGAGTCG955.2750693E-1177.8798844
GGATGCG305.1054114E-576.4996143
CCTTAGC60850.076.46818543
AACTACC5400.075.35644
GGCACGG1051.2732926E-1070.4627544
CCGTTAA451.4188117E-1069.6230642
CCGATTT400.002306253469.3617744
Produced by FastQC (version 0.11.5)