##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR841038.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2054885
Sequences flagged as poor quality	0
Sequence length	24-50
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.414695226253535	39.0	35.0	39.0	32.0	39.0
2	37.13717312647666	39.0	37.0	39.0	33.0	39.0
3	37.38315331514902	39.0	37.0	39.0	34.0	39.0
4	37.907015234429174	39.0	37.0	39.0	35.0	41.0
5	38.72034542078997	40.0	38.0	41.0	35.0	41.0
6	38.530876423741475	40.0	37.0	41.0	35.0	41.0
7	38.69773101657757	40.0	38.0	41.0	35.0	41.0
8	38.536562386702904	40.0	37.0	41.0	35.0	41.0
9	38.52745287449176	40.0	37.0	41.0	35.0	41.0
10	38.51473294125949	40.0	37.0	41.0	35.0	41.0
11	38.47064580256316	40.0	37.0	41.0	35.0	41.0
12	38.496358677006256	40.0	37.0	41.0	35.0	41.0
13	38.41332726648937	40.0	37.0	41.0	34.0	41.0
14	38.35975589874859	40.0	37.0	41.0	35.0	41.0
15	38.10790628186005	40.0	36.0	41.0	34.0	41.0
16	38.120391652087584	40.0	36.0	41.0	34.0	41.0
17	38.11951617730433	40.0	36.0	41.0	34.0	41.0
18	37.99670979154551	39.0	35.0	41.0	34.0	41.0
19	38.044325108217734	40.0	35.0	41.0	34.0	41.0
20	38.003858610092536	40.0	35.0	41.0	34.0	41.0
21	37.8898142718449	39.0	35.0	41.0	34.0	41.0
22	37.78051034486115	39.0	35.0	41.0	34.0	41.0
23	37.72545860230621	39.0	35.0	41.0	33.0	41.0
24	37.54265226521192	39.0	35.0	41.0	33.0	41.0
25	37.489123997160675	39.0	35.0	40.0	33.0	41.0
26	37.355991495903055	39.0	35.0	40.0	33.0	41.0
27	37.26496383338704	38.0	35.0	40.0	33.0	41.0
28	37.17818619808693	38.0	35.0	40.0	33.0	41.0
29	36.98347859699632	38.0	35.0	40.0	32.0	41.0
30	36.978924637266644	38.0	35.0	40.0	32.0	41.0
31	36.91976080040659	38.0	35.0	40.0	32.0	41.0
32	36.821942182637486	38.0	35.0	40.0	32.0	41.0
33	36.70114257478669	38.0	35.0	40.0	32.0	41.0
34	36.63005969797361	38.0	35.0	40.0	32.0	41.0
35	36.52670530359117	37.0	35.0	40.0	31.0	41.0
36	36.51299868569172	37.0	35.0	40.0	32.0	41.0
37	36.37511441641429	37.0	34.0	40.0	31.0	41.0
38	36.32524719491575	37.0	34.0	40.0	31.0	41.0
39	36.149636054030196	37.0	34.0	40.0	31.0	41.0
40	36.083257410516005	36.0	34.0	40.0	31.0	41.0
41	35.8774970675008	36.0	34.0	39.0	31.0	41.0
42	35.81256813602262	36.0	34.0	39.0	31.0	41.0
43	35.54139089006614	36.0	34.0	39.0	30.0	41.0
44	35.389519292908666	36.0	34.0	39.0	29.0	41.0
45	35.03950179989975	35.0	33.0	39.0	29.0	41.0
46	34.78736807141937	35.0	33.0	39.0	28.0	41.0
47	34.68891684089454	35.0	33.0	38.0	28.0	40.0
48	35.50361693410262	36.0	34.0	39.0	31.0	41.0
49	36.271053596377364	36.0	35.0	39.0	32.0	41.0
50	36.274009919720434	37.0	35.0	39.0	31.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	8.0
21	25.0
22	61.0
23	162.0
24	364.0
25	780.0
26	1423.0
27	2736.0
28	5399.0
29	10683.0
30	19434.0
31	36874.0
32	67723.0
33	119917.0
34	195012.0
35	138808.0
36	229458.0
37	339875.0
38	306633.0
39	285311.0
40	294199.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	47.06336364322091	19.307309168152962	33.62932718862613
2	22.19077953267458	21.67892607128866	35.512060285612094	20.618234110424673
3	20.330626774734352	25.824608189752713	28.76968784141205	25.075077194100885
4	18.881835236521752	27.277438883441167	32.10140713470583	21.739318745331246
5	16.088929550802114	28.507970032386243	31.344576460483193	24.058523956328457
6	18.049866537543462	29.96148202940797	26.56893208135735	25.41971935169121
7	22.76521557167433	26.80680427371848	22.93520075332683	27.492779401280366
8	22.077439856731644	27.581786815320562	30.162661170819778	20.178112157128012
9	21.78642600437494	30.954919618372806	26.258160432335632	21.000493944916627
10	25.928847599744024	24.80751964221842	25.478554760972028	23.785077997065528
11	23.828292094204787	25.531161111205737	24.888108093640277	25.752438700949202
12	22.258131233621345	26.067103511875363	25.411105730977646	26.263659523525646
13	22.500626555743995	24.77111857841193	25.470427785496515	27.257827080347564
14	19.19134160792453	27.155728909403688	25.368524272647864	28.284405210023916
15	20.811188947313354	34.04866939025785	24.438885874391996	20.7012557880368
16	19.508439645040966	35.791297323207864	23.08231360879076	21.617949422960407
17	20.584752918046508	35.68613328726425	22.1260070514895	21.60310674319974
18	20.088569433325954	28.471374310484528	23.059051966411744	28.381004289777774
19	22.299885395046438	27.113828754407184	23.328166783055988	27.258119067490394
20	21.8456507298462	25.10938568338374	25.824267538086072	27.22069604868399
21	21.608508505342147	32.01629288257007	23.803521851587803	22.57167676049998
22	23.85432761444071	27.564608238417236	22.680149984062368	25.900914163079687
23	24.14105898870253	27.46241273842575	27.06969003131562	21.326838241556096
24	22.66292274263523	28.289904301213937	24.3196577910686	24.72751516508223
25	23.116165987942168	28.27359039135779	23.451878653563437	25.158364967136603
26	23.688326032534494	33.70721524414701	22.559320225485024	20.04513849783347
27	23.718653983407638	28.551178882662587	23.53785827309715	24.192308860832625
28	24.515494714396354	27.067018034419288	22.245803200531075	26.171684050653283
29	22.67789359476898	28.429603208990212	27.27722950812178	21.615273688119025
30	24.472065162067114	29.60879078972255	27.21524015723614	18.703903890974196
31	24.825850762906278	31.168383298425066	23.827255538315416	20.178510400353236
32	22.818402280297516	27.670236624125643	24.83977407584418	24.671587019732662
33	22.24431745084891	26.846700479892966	29.97606048783254	20.932921581425585
34	22.421021637277587	31.506553138491583	25.317375107641897	20.755050116588933
35	21.81712411628547	26.17503495472518	24.84145963055237	27.166381298436974
36	20.83106289658971	27.02915428307511	26.31183022116063	25.82795259917455
37	22.208643789493205	35.41884763129722	22.628359314439486	19.744149264770094
38	27.61123432381165	26.827811169594224	22.322491037337695	23.23846346925643
39	20.56407942484479	33.08331882932759	24.269775598024253	22.082826147803367
40	19.61414897073952	28.212112498096204	26.047143808354495	26.12659472280978
41	20.171984413322434	33.940956794511784	23.784623484875414	22.10243530729037
42	20.943268791529693	34.01193308727296	22.655770508067025	22.38902761313032
43	21.392376763040943	26.25211655703696	25.39278305120139	26.962723628720713
44	21.37739790897304	26.542538819844335	24.518046114602072	27.562017156580552
45	21.908470920606955	25.618360496377417	30.75231252942874	21.72085605358689
46	21.08328870752357	28.31883841841611	28.050880201731253	22.54699267232906
47	20.863474148306974	32.66467063444847	25.579241912467594	20.89261330477696
48	25.751380998118368	26.650265165072096	24.82678152835256	22.771572308456978
49	19.527721117083093	27.318822218793482	24.876034578673277	28.277422085450148
50	20.42233335855685	28.35948553202125	27.389297433392723	23.82888367602918
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	73206.0
1	65376.5
2	47747.5
3	36566.0
4	32214.0
5	21811.5
6	10647.0
7	7116.0
8	7226.0
9	6891.0
10	6348.0
11	5899.0
12	5495.0
13	4969.5
14	4641.5
15	4653.5
16	4745.5
17	5088.0
18	6062.0
19	7392.0
20	8277.0
21	9155.5
22	10512.5
23	12419.0
24	14068.5
25	15988.5
26	18948.0
27	22416.5
28	25456.0
29	29359.0
30	34607.0
31	43365.0
32	56700.5
33	61672.0
34	62878.5
35	68643.5
36	71949.5
37	75125.5
38	78847.5
39	83504.5
40	90559.0
41	100544.0
42	109563.0
43	114037.0
44	116636.0
45	123733.5
46	135869.5
47	141500.5
48	155605.0
49	181910.0
50	212239.0
51	230964.5
52	211511.0
53	176532.5
54	157158.0
55	147724.5
56	142666.5
57	139107.5
58	132489.0
59	117759.5
60	95748.0
61	77183.5
62	63161.0
63	49201.5
64	35062.5
65	26215.5
66	18899.5
67	12840.0
68	9430.5
69	6672.5
70	4730.0
71	3447.5
72	2579.0
73	1890.5
74	1386.0
75	1016.5
76	760.5
77	481.0
78	253.5
79	166.5
80	106.5
81	82.5
82	61.5
83	36.0
84	15.5
85	8.5
86	5.0
87	3.0
88	1.5
89	1.0
90	0.5
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
24	13556.0
25	16893.0
26	16645.0
27	19371.0
28	19701.0
29	17768.0
30	16834.0
31	15420.0
32	14314.0
33	12694.0
34	12830.0
35	12478.0
36	11315.0
37	10469.0
38	11770.0
39	14094.0
40	18221.0
41	16397.0
42	21819.0
43	33863.0
44	82508.0
45	170695.0
46	239779.0
47	193270.0
48	335734.0
49	270346.0
50	436101.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	12.120670315671337
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.35096603020072	8.40580195325385
2	11.403150163992606	2.7642764739569583
3	4.225280585779154	1.5363969891430735
4	2.1237476622536864	1.029649809914186
5	1.3128958125556252	0.795658865140608
6	0.8979633490025223	0.6530350625289414
7	0.6865528008235423	0.5825036107158047
8	0.5519612920177603	0.5352112678047413
9	0.4183402894375738	0.456350825523182
>10	5.0447622939652685	14.819996791729704
>50	2.327167658726208	20.287493385797365
>100	1.5666178018972778	30.908388675733615
>500	0.05833386455583658	4.936677666402638
>1k	0.02872500906158619	6.757427061354663
>5k	0.001767692865328381	1.2144578439558507
>10k+	0.001767692865328381	4.316673717044858
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGC	34082	1.658584300337975	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTA	21409	1.0418587901512737	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCT	14581	0.7095774216075352	No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATCAT	10517	0.5118047968621116	No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATC	5914	0.2878019937855403	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAA	5763	0.28045365069091455	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAA	5514	0.26833618426335293	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAA	5482	0.26677891950157795	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA	4432	0.2156811695058361	No Hit
TCAGTTCTTTGACCATTCGTTGGAGCTCCGGTTTTCAGCCTCTTTCCG	4163	0.2025904126021651	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAA	4131	0.2010331478403901	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGCC	3997	0.19451210165045732	No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTA	3893	0.1894509911746886	No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3736	0.18181066093723006	No Hit
AAAAAAAAAAAAAAAAAAAAAAAA	3609	0.17563026641393556	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACC	3596	0.1749976276044645	No Hit
AAGATAAAATTTGAAATCTGGTTAGGCTGGTGTTAGGGTTCTTTGTTTTT	3160	0.15377989522528024	No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCTA	2967	0.14438764213082483	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2824	0.13742861522664285	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTT	2584	0.12574912951333042	No Hit
ATAGGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACATGC	2543	0.12375388403730622	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAG	2508	0.12205062570411482	No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2364	0.11504293427612737	No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTTTT	2356	0.11465361808568363	No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2276	0.11076045618124615	No Hit
CCTTTCCGGCGGTGACGACCTACGCACACGAGAACATGCCTCTCGCAAA	2264	0.11017648189558053	No Hit
TCAGTTCTTTGACCATTCGTTGGAGCTCCGGTTTTCAGCCTCTTTC	2204	0.10725661046725242	No Hit
AAAGATAAAATTTGAAATCTGGTTAGGCTGGTGTTAGGGTTCTTTGTTTT	2125	0.10341211308662042	No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2113	0.10282813880095479	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.10200084189626184	0.0	0.0	0.0	0.0
2	0.17533827927110276	0.0	0.0	0.0	0.0
3	0.22132625426727043	0.0	0.0	0.0	0.0
4	0.2582139633118155	0.0	0.0	0.0	0.0
5	0.28906727140448246	0.0	0.0	0.0	0.0
6	0.3418196152096103	0.0	0.0	0.0	0.0
7	0.3750088204449397	0.0	0.0	0.0	0.0
8	0.4120911875847067	0.0	0.0	0.0	0.0
9	0.4492708837720846	0.0	0.0	0.0	0.0
10	0.48572061210238043	0.0	0.0	0.0	0.0
11	0.5227056501945364	0.0	0.0	0.0	0.0
12	0.5620265854293549	0.0	0.0	0.0	0.0
13	0.6013961851879789	0.0	0.0	0.0	0.0
14	0.6331254547091443	0.0	0.0	0.0	0.0
15	0.6647087306588934	0.0	0.0	0.0	0.0
16	0.6973139616085572	0.0	0.0	0.0	0.0
17	0.7317197799390234	0.0	0.0	0.0	0.0
18	0.7651523077933802	0.0	0.0	0.0	0.0
19	0.7925017701720535	0.0	0.0	0.0	0.0
20	0.8203865423125868	0.0	0.0	0.0	0.0
21	0.8458380882628468	0.0	0.0	0.0	0.0
22	0.8716302858797451	0.0	0.0	0.0	0.0
23	0.8992717354012512	0.0	0.0	0.0	0.0
24	0.9244799587324838	0.0	0.0	0.0	0.0
25	0.9525107244444336	0.0	0.0	0.0	0.0
26	0.9801521739659397	0.0	0.0	0.0	0.0
27	1.008864243011166	0.0	0.0	0.0	0.0
28	1.0385496025325018	0.0	0.0	0.0	0.0
29	1.0680403039586157	0.0	0.0	0.0	0.0
30	1.0988936120512827	0.0	0.0	0.0	0.0
31	1.1278003391917308	0.0	0.0	0.0	0.0
32	1.1548578144275714	0.0	0.0	0.0	0.0
33	1.183180567282354	0.0	0.0	0.0	0.0
34	1.2120872944228023	0.0	0.0	0.0	0.0
35	1.2370035306112022	0.0	0.0	0.0	0.0
36	1.2571993079904715	0.0	0.0	0.0	0.0
37	1.2798769760838198	0.0	0.0	0.0	0.0
38	1.2930163975112963	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCGACAA	10	0.0034231339	184.96472	44
ATCGACT	20	1.4665237E-4	138.72354	44
GACTAAG	40	1.4330726E-7	115.60295	44
ACCGATT	15	3.1219862E-4	114.74942	43
ATCCCGT	20	6.8214013E-6	114.74941	43
CGAGTGC	50	4.1763997E-9	110.97884	44
GATGCGT	25	3.5649465E-4	110.97884	44
CTATCAT	2060	0.0	106.39962	44
ACGAACA	150	0.0	104.81335	44
TACGCTT	20	9.798558E-4	86.06206	43
CCCGAGA	140	0.0	85.87648	44
ACGATTG	15	0.001424457	78.32595	42
CGCTATA	20	4.5429144E-5	78.32594	42
CGAGTCG	95	5.2750693E-11	77.87988	44
GGATGCG	30	5.1054114E-5	76.49961	43
CCTTAGC	6085	0.0	76.468185	43
AACTACC	540	0.0	75.356	44
GGCACGG	105	1.2732926E-10	70.46275	44
CCGTTAA	45	1.4188117E-10	69.62306	42
CCGATTT	40	0.0023062534	69.36177	44
>>END_MODULE