FastQCFastQC Report
Tue 24 May 2016
ERR841037.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR841037.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2142633
Sequences flagged as poor quality0
Sequence length24-50
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGC384191.793074222230312No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTA244061.1390658129507012No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATCAT219351.0237404165809076No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCT142760.6662830265379092No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATC123100.5745267621659892No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTA81490.38032644881321254No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCTA60160.28077603584001554No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAGAA51010.23807156895277914No Hit
TCAGTTCTTTGACCATTCGTTGGAGCTCCGGTTTTCAGCCTCTTTCCG40060.18696622333362736No Hit
ACATACCCATGGCCAACCTCCTACTCCTCATTGTACCCATTCTAATCGCA40050.18691955178511674No Hit
CCTTTCCGGCGGTGACGACCTACGCACACGAGAACATGCCTCTCGCAAA39800.1857527630723507No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACC39790.18570609152384007No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTC38690.18057222118766958No Hit
ATAGGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACATGC35750.16685078592554115No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAG31230.14575524599873146No Hit
CTTTTTCGCAACGGGTTTGCCGCCAGAACACAGGTGTCGTGAAAACTAC30610.14286160999107173No Hit
TCAGTTCTTTGACCATTCGTTGGAGCTCCGGTTTTCAGCCTCTTTCCGG29430.1373543672668161No Hit
CAGAGTGTTGCTTAACACAAAGCACCCAACTTACACTTAGGAGATTTCAA29170.13614090700553944No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTTTTT27010.126059852527241No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTTTT26700.12461303452341115No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAA25760.1202259089634109No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25740.12013256586638962No Hit
TCCCTGGTGTGATTCCGTCCTGCGCGGTTGTTCTCTGGAGCAGCGTTCT25690.11989920812383642No Hit
CCTTTCCGGCGGTGACGACCTACGCACACGAGAACATGCCTCTCGCA25590.11943249263873No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAA25560.11929247799319809No Hit
ATTAATCCCCTGGCCCAACCCGTCATCTACTCTACCATCTTTGCAGGCAC25540.1191991348961768No Hit
ACATACCCATGGCCAACCTCCTACTCCTCATTGTACCCATTCTAATCG25000.1166788712766022No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACACA24100.11247843191064452No Hit
ATAGGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACAT23880.11145165784341043No Hit
TCAGTTCTTTGACCATTCGTTGGAGCTCCGGTTTTCAGCCTCTTTC23770.11093827080979338No Hit
CTCTTCCTGCTCTCCATCATGGCGCAGGATCAAGGTGAAAAGGAGAACC23370.10907140886936774No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAA22700.10594441511915478No Hit
CCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCTAG22000.10267740672340994No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCT21770.1016039611076652No Hit
CGGCACAGCTGGTTTGAGCAACTGAACTGGAAACAAGATGCAGGACCC21640.10099723097702687No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACT21460.10015714310383533No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTTT21460.10015714310383533No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAA21440.10006380000681404No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTACA100.00189124225.5195344
GTACGCT100.00189124225.5195344
CTATCAT29550.0179.3471844
AATCGCA6200.0160.0461344
TATTTCG150.0063617625150.3463744
ACATTGG4400.0112.75976644
CGTTCAC356.1765494E-496.6512344
TCGTGCG1050.094.76623543
TATAGAC604.925205E-793.96648444
CCTATCA30550.090.2409543
GGATCTA502.2527518E-590.2078244
CGTATTC209.0513186E-487.7981343
AGCCCGT209.0513186E-487.7981343
GATAGAC209.0513186E-487.7981343
ACTTCTA14900.086.27257544
CCGCCTA400.001049966984.56982444
CTATTCA1700.079.5951344
CGAGGCC1207.094059E-1175.1731944
TCACGCT906.238952E-875.1731844
CCTTAGC65800.074.4549543
Produced by FastQC (version 0.11.5)