##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR841037.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2142633
Sequences flagged as poor quality	0
Sequence length	24-50
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	37.347140644244725	39.0	37.0	39.0	34.0	39.0
2	37.87097463728039	39.0	38.0	39.0	35.0	39.0
3	38.0669069317984	39.0	38.0	39.0	35.0	39.0
4	38.672453005251015	39.0	38.0	40.0	35.0	41.0
5	39.31617593866985	40.0	39.0	41.0	36.0	41.0
6	39.22583148864038	40.0	39.0	41.0	35.0	41.0
7	39.261387741157726	40.0	39.0	41.0	35.0	41.0
8	39.22074895700757	40.0	38.0	41.0	35.0	41.0
9	39.212803125873634	40.0	39.0	41.0	35.0	41.0
10	39.179993027270655	40.0	39.0	41.0	35.0	41.0
11	39.10575212833929	40.0	38.0	41.0	35.0	41.0
12	39.045668576933146	40.0	38.0	41.0	35.0	41.0
13	38.92404018793699	40.0	38.0	41.0	35.0	41.0
14	38.895580344370686	40.0	38.0	41.0	35.0	41.0
15	38.85001771185266	40.0	38.0	41.0	35.0	41.0
16	38.77250373722425	40.0	38.0	41.0	35.0	41.0
17	38.740281233417015	40.0	38.0	41.0	35.0	41.0
18	38.682676874667756	40.0	38.0	41.0	35.0	41.0
19	38.66633996582709	40.0	38.0	41.0	35.0	41.0
20	38.55047831336491	40.0	38.0	41.0	35.0	41.0
21	38.469914353041325	40.0	38.0	41.0	35.0	41.0
22	38.42278589007077	40.0	37.0	41.0	35.0	41.0
23	38.30317417868576	40.0	37.0	41.0	34.0	41.0
24	38.076495134724425	40.0	37.0	41.0	34.0	41.0
25	38.02367171023672	39.0	37.0	41.0	34.0	41.0
26	37.875710395061866	39.0	36.0	41.0	33.0	41.0
27	37.74554001825393	39.0	36.0	41.0	33.0	41.0
28	37.64973004806286	39.0	36.0	41.0	33.0	41.0
29	37.606833214320076	39.0	36.0	41.0	33.0	41.0
30	37.54467082156289	39.0	36.0	41.0	33.0	41.0
31	37.49125722010569	39.0	35.0	41.0	33.0	41.0
32	37.40444423090069	39.0	35.0	40.0	33.0	41.0
33	37.2679142281512	38.0	35.0	40.0	32.0	41.0
34	37.202168154867344	38.0	35.0	40.0	32.0	41.0
35	37.04081588269448	38.0	35.0	40.0	32.0	41.0
36	36.99014899461094	38.0	35.0	40.0	32.0	41.0
37	36.934204582123265	38.0	35.0	40.0	32.0	41.0
38	36.709018669870886	38.0	35.0	40.0	31.0	41.0
39	36.66063249896968	38.0	35.0	40.0	31.0	41.0
40	36.47297373493523	37.0	34.0	40.0	31.0	41.0
41	36.36202150401753	37.0	34.0	40.0	31.0	41.0
42	36.22114995619973	37.0	34.0	40.0	31.0	41.0
43	36.04833452221272	37.0	34.0	40.0	30.0	41.0
44	35.7716806168546	36.0	34.0	40.0	29.0	41.0
45	35.551806659481144	36.0	33.0	40.0	29.0	41.0
46	35.22302305126483	36.0	33.0	40.0	28.0	41.0
47	35.347724877121244	36.0	33.0	39.0	29.0	41.0
48	36.28546892041828	37.0	34.0	40.0	31.0	41.0
49	36.853925105242965	38.0	35.0	40.0	32.0	41.0
50	37.01965221723438	38.0	35.0	40.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	3.0
21	12.0
22	36.0
23	94.0
24	189.0
25	343.0
26	719.0
27	1398.0
28	2924.0
29	6493.0
30	12791.0
31	24880.0
32	45845.0
33	77181.0
34	116656.0
35	133588.0
36	231331.0
37	377144.0
38	364630.0
39	334043.0
40	412333.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	42.80733098015386	19.096364146356375	38.096304873489764
2	21.927833651399936	19.57521423407555	35.04851274109939	23.448439373425124
3	21.81306831361227	23.38804638965236	28.41317201779306	26.385713278942312
4	19.557852418029594	25.602611366482268	32.99664478237757	21.84289143311057
5	16.77795497409029	26.051544991606125	31.21369828617407	25.95680174812952
6	18.79215899316402	26.623598161701047	28.115640896037725	26.46860194909721
7	23.51699987818726	22.24730040095527	22.949473848297867	31.286225872559605
8	22.566160420379973	24.24246242823666	29.315846437537367	23.875530713846
9	21.572943196525024	26.8027702364334	27.976466338378998	23.647820228662585
10	28.02514476347559	19.84170877607131	28.30522072608795	23.82792573436515
11	23.988009145756646	22.386661644808047	26.055978788714633	27.569350420720674
12	22.826867690360412	25.163292080351606	25.667344804266527	26.342495425021458
13	26.3922472957338	21.801447098033123	25.658803910889077	26.147501695344
14	19.871718581763652	24.341032738691133	23.762725581095783	32.02452309844943
15	22.243473333977402	32.06690086449709	23.55522387641747	22.134401925108033
16	20.35294891845687	31.889315622414106	22.267975896945487	25.48975956218354
17	20.765618750387958	34.15288572518019	21.02436581533095	24.057129709100906
18	20.385058943832192	27.925454335856863	21.93371426651228	29.755772453798667
19	23.721234574469825	24.557542052231998	22.957314668447655	28.76390870485053
20	22.27903705394251	23.314211999908522	27.14757963683001	27.25917130931895
21	21.888349521359935	30.57677166364935	24.38238373067156	23.152495084319153
22	26.976481739990003	24.342526228243475	22.131228259809312	26.549763771957213
23	26.1483884547657	24.48356764784263	26.334841291065715	23.033202606325958
24	23.246584926116608	26.897793509201062	24.583584776300935	25.272036788381396
25	23.009829658669727	25.042024536134534	24.1557784558435	27.792367349352244
26	23.899135713570224	31.310113077317066	24.615153456673227	20.175597752439486
27	24.038504548729318	27.830809539626145	22.35172969726848	25.77895621437606
28	23.880603456957797	25.838030756009296	22.759888086722242	27.52147770031067
29	22.826952466088475	29.09480143318715	25.74510599270836	22.333140108016014
30	24.837976108940502	29.705639333234213	25.38886952537176	20.067515032453525
31	25.730416615460243	29.406267666216053	23.086641268280694	21.776674450043014
32	21.22875390941936	28.742417683008377	25.70706375238875	24.321764655183518
33	23.35883166905277	24.274834919741142	29.820473346551736	22.545860064654356
34	23.876437383761804	28.927224147695433	24.67478430763618	22.52155416090658
35	21.146411382584226	24.859649726177878	25.312513276377253	28.68142561486064
36	21.282937024129847	24.95722100740009	26.668788009154014	27.091053959316046
37	20.804714270348345	33.76178695636585	22.444567371963306	22.988931401322496
38	25.921204632790833	25.797800259071895	22.25601744458654	26.02497766355073
39	20.75743319777192	31.618468492987358	25.227227367335143	22.396870941905583
40	20.703447224490393	25.965701087399157	27.335235115871598	25.99561657223885
41	20.538811487062837	30.632904727303362	23.733242767931422	25.095041017702375
42	20.47750258819782	31.590294921823155	22.821268349658887	25.11093414032014
43	21.457850602678654	24.55070825543892	24.497409960501294	29.494031181381132
44	20.05691348329671	26.46225240582597	22.933725890436072	30.547108220441245
45	22.19617630276028	24.957953163455276	30.920592286898334	21.925278246886112
46	18.94014498133289	28.763017083510213	27.49717701803393	24.79966091712297
47	20.178678459148987	30.645935413347992	27.045957152994294	22.129428974508734
48	25.23130931204392	26.08188656016305	22.94098183556135	25.745822292231686
49	18.774024345128154	28.71630402941989	22.273963145365254	30.2357084800867
50	16.81197364864507	30.86426296235445	29.16880873007881	23.15495465892167
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	32530.0
1	30825.5
2	35491.0
3	41250.5
4	39033.0
5	27341.5
6	12433.0
7	8120.0
8	8537.0
9	8206.5
10	7552.0
11	6505.5
12	5411.5
13	4856.0
14	5085.0
15	5360.5
16	5237.0
17	5438.0
18	6077.5
19	6346.5
20	6361.0
21	7594.5
22	9101.5
23	10015.5
24	10948.5
25	12648.0
26	15201.5
27	17922.0
28	20249.0
29	23282.5
30	28406.0
31	37691.5
32	51384.5
33	56800.5
34	55418.0
35	58900.5
36	61529.0
37	64089.0
38	68258.5
39	73205.5
40	82262.0
41	96431.5
42	106053.0
43	105635.5
44	107364.5
45	116088.0
46	133093.5
47	149281.5
48	169232.0
49	197499.0
50	237714.5
51	265774.5
52	241908.0
53	199725.5
54	180314.0
55	171809.0
56	162335.0
57	154893.5
58	146806.5
59	133509.0
60	113868.5
61	97822.0
62	85395.5
63	69381.5
64	54709.0
65	45389.5
66	32834.0
67	20835.0
68	14687.5
69	10986.5
70	8624.5
71	6211.5
72	4177.5
73	2751.5
74	1930.0
75	1522.5
76	1191.5
77	914.0
78	504.0
79	205.0
80	142.5
81	96.0
82	55.5
83	67.5
84	90.5
85	83.0
86	78.0
87	39.0
88	1.0
89	1.0
90	0.5
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
24	11735.0
25	14470.0
26	14962.0
27	16280.0
28	16789.0
29	17002.0
30	17145.0
31	15408.0
32	15574.0
33	13281.0
34	12791.0
35	13031.0
36	11795.0
37	11596.0
38	11759.0
39	13619.0
40	16216.0
41	15630.0
42	20451.0
43	35764.0
44	92365.0
45	196703.0
46	231898.0
47	245560.0
48	333682.0
49	349765.0
50	377362.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	11.465284838615169
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.32060654539703	7.94780499228548
2	11.448246891108791	2.6251482301870577
3	4.264903437553387	1.466949981622157
4	2.172660371157691	0.9964068005157712
5	1.3328611861151796	0.7640816574572501
6	0.9130068542265916	0.6280730185989523
7	0.6919238277303246	0.5553172640087145
8	0.5542904100441499	0.5084077947575183
9	0.44795305756978504	0.46223184594295486
>10	4.754158675930946	12.891402719728676
>50	2.272250404849606	18.847167636625795
>100	1.7288668433620191	32.56708790656186
>500	0.054694089952039185	4.3590447941485495
>1k	0.04002006581856525	8.721964249463111
>5k	0.0013340021939521752	0.9822266055843317
>10k+	0.0022233369899202922	5.676684502511834
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGC	38419	1.793074222230312	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTA	24406	1.1390658129507012	No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATCAT	21935	1.0237404165809076	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCT	14276	0.6662830265379092	No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATC	12310	0.5745267621659892	No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTA	8149	0.38032644881321254	No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCTA	6016	0.28077603584001554	No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAGAA	5101	0.23807156895277914	No Hit
TCAGTTCTTTGACCATTCGTTGGAGCTCCGGTTTTCAGCCTCTTTCCG	4006	0.18696622333362736	No Hit
ACATACCCATGGCCAACCTCCTACTCCTCATTGTACCCATTCTAATCGCA	4005	0.18691955178511674	No Hit
CCTTTCCGGCGGTGACGACCTACGCACACGAGAACATGCCTCTCGCAAA	3980	0.1857527630723507	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACC	3979	0.18570609152384007	No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTC	3869	0.18057222118766958	No Hit
ATAGGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACATGC	3575	0.16685078592554115	No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAG	3123	0.14575524599873146	No Hit
CTTTTTCGCAACGGGTTTGCCGCCAGAACACAGGTGTCGTGAAAACTAC	3061	0.14286160999107173	No Hit
TCAGTTCTTTGACCATTCGTTGGAGCTCCGGTTTTCAGCCTCTTTCCGG	2943	0.1373543672668161	No Hit
CAGAGTGTTGCTTAACACAAAGCACCCAACTTACACTTAGGAGATTTCAA	2917	0.13614090700553944	No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2701	0.126059852527241	No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTTTT	2670	0.12461303452341115	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAA	2576	0.1202259089634109	No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2574	0.12013256586638962	No Hit
TCCCTGGTGTGATTCCGTCCTGCGCGGTTGTTCTCTGGAGCAGCGTTCT	2569	0.11989920812383642	No Hit
CCTTTCCGGCGGTGACGACCTACGCACACGAGAACATGCCTCTCGCA	2559	0.11943249263873	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAA	2556	0.11929247799319809	No Hit
ATTAATCCCCTGGCCCAACCCGTCATCTACTCTACCATCTTTGCAGGCAC	2554	0.1191991348961768	No Hit
ACATACCCATGGCCAACCTCCTACTCCTCATTGTACCCATTCTAATCG	2500	0.1166788712766022	No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACACA	2410	0.11247843191064452	No Hit
ATAGGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACAT	2388	0.11145165784341043	No Hit
TCAGTTCTTTGACCATTCGTTGGAGCTCCGGTTTTCAGCCTCTTTC	2377	0.11093827080979338	No Hit
CTCTTCCTGCTCTCCATCATGGCGCAGGATCAAGGTGAAAAGGAGAACC	2337	0.10907140886936774	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAA	2270	0.10594441511915478	No Hit
CCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCTAG	2200	0.10267740672340994	No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCT	2177	0.1016039611076652	No Hit
CGGCACAGCTGGTTTGAGCAACTGAACTGGAAACAAGATGCAGGACCC	2164	0.10099723097702687	No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACT	2146	0.10015714310383533	No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTTT	2146	0.10015714310383533	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2144	0.10006380000681404	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.04293782462978961	0.0	0.0	0.0	0.0
2	0.0987569966485161	0.0	0.0	0.0	0.0
3	0.1364209362966033	0.0	0.0	0.0	0.0
4	0.16470389469405167	0.0	0.0	0.0	0.0
5	0.1871062379791593	0.0	0.0	0.0	0.0
6	0.22906396009022545	0.0	0.0	0.0	0.0
7	0.2592604519766101	0.0	0.0	0.0	0.0
8	0.29939798369576126	0.0	0.0	0.0	0.0
9	0.3301078626157629	0.0	0.0	0.0	0.0
10	0.3589042080468284	0.0	0.0	0.0	0.0
11	0.39106090497065993	0.0	0.0	0.0	0.0
12	0.42601789480512997	0.0	0.0	0.0	0.0
13	0.45719448921023803	0.0	0.0	0.0	0.0
14	0.4872509664510908	0.0	0.0	0.0	0.0
15	0.5187542616957734	0.0	0.0	0.0	4.667154851064088E-5
16	0.5511909879106688	0.0	0.0	0.0	4.667154851064088E-5
17	0.5896016723349262	0.0	0.0	0.0	4.667154851064088E-5
18	0.6323528107706733	0.0	0.0	0.0	4.667154851064088E-5
19	0.661895900977909	0.0	0.0	0.0	4.667154851064088E-5
20	0.6910189472485488	0.0	0.0	0.0	4.667154851064088E-5
21	0.7167816420264226	0.0	0.0	0.0	4.667154851064088E-5
22	0.7388572844719558	0.0	0.0	0.0	4.667154851064088E-5
23	0.7633598474400423	0.0	0.0	0.0	4.667154851064088E-5
24	0.7889358560238734	0.0	0.0	0.0	4.667154851064088E-5
25	0.8117582432455768	0.0	0.0	0.0	4.667154851064088E-5
26	0.8432615384902594	0.0	0.0	0.0	4.667154851064088E-5
27	0.8709844383055801	0.0	0.0	0.0	4.667154851064088E-5
28	0.8956270159191985	0.0	0.0	0.0	4.667154851064088E-5
29	0.9274570120034555	0.0	0.0	0.0	4.667154851064088E-5
30	0.9565800582740954	0.0	0.0	0.0	4.667154851064088E-5
31	0.9868698932575014	0.0	0.0	0.0	4.667154851064088E-5
32	1.0162262972706946	0.0	0.0	0.0	4.667154851064088E-5
33	1.0433424669553768	0.0	0.0	0.0	4.667154851064088E-5
34	1.0701786073489954	0.0	0.0	0.0	4.667154851064088E-5
35	1.093234352313252	0.0	0.0	0.0	4.667154851064088E-5
36	1.1132564466243169	0.0	0.0	0.0	4.667154851064088E-5
37	1.1322517668681478	0.0	0.0	0.0	4.667154851064088E-5
38	1.1452731289026166	0.0	0.0	0.0	4.667154851064088E-5
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTACA	10	0.00189124	225.51953	44
GTACGCT	10	0.00189124	225.51953	44
CTATCAT	2955	0.0	179.34718	44
AATCGCA	620	0.0	160.04613	44
TATTTCG	15	0.0063617625	150.34637	44
ACATTGG	440	0.0	112.759766	44
CGTTCAC	35	6.1765494E-4	96.65123	44
TCGTGCG	105	0.0	94.766235	43
TATAGAC	60	4.925205E-7	93.966484	44
CCTATCA	3055	0.0	90.24095	43
GGATCTA	50	2.2527518E-5	90.20782	44
CGTATTC	20	9.0513186E-4	87.79813	43
AGCCCGT	20	9.0513186E-4	87.79813	43
GATAGAC	20	9.0513186E-4	87.79813	43
ACTTCTA	1490	0.0	86.272575	44
CCGCCTA	40	0.0010499669	84.569824	44
CTATTCA	170	0.0	79.59513	44
CGAGGCC	120	7.094059E-11	75.17319	44
TCACGCT	90	6.238952E-8	75.17318	44
CCTTAGC	6580	0.0	74.45495	43
>>END_MODULE