FastQCFastQC Report
Tue 24 May 2016
ERR841036.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR841036.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3276102
Sequences flagged as poor quality0
Sequence length24-50
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGC360001.098866885096984No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTA243950.7446349350539146No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCT141840.4329535527282118No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACACA124970.38145942952936146No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAGAA85630.26137769825237434No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACA80360.24529150801776015No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAA78210.23872883078731982No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAA76640.2339365502050913No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAA69900.21336332018966442No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATCAT64690.19746027443589975No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAA61040.18631898518422196No Hit
CAGAGTGTTGCTTAACACAAAGCACCCAACTTACACTTAGGAGATTTCAA57810.1764597072984907No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGCC56600.17276629360135917No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA56580.17270524544107602No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCTA52460.16012932442274386No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAG51320.15664957928660342No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA47950.14636296427888998No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAA44920.13711416799599035No Hit
CACTTAGGAGATTTCAACTTAACTTGACCGCTCTGACCAAAAAAAAAAAA44730.13653421047330028No Hit
AAAAAAAAAAAAAAAAAAAAAAAA43240.13198612253220443No Hit
AACACAAAGCACCCAACTTACACTTAGGAGATTTCAACTTAACTTGACCG42730.13042939444498372No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAA40000.12209632056633156No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATC39360.12014277943727028No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAG37490.11443477645079426No Hit
CAGAGTGTTGCTTAACACAAAGCACCCAACTTACACTTAGGAGATTTC37370.11406848748909527No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACC36140.11031402563168058No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCC34690.10588803401115107No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTT34090.10405658920265609No Hit
CAAAGCACCCAACTTACACTTAGGAGATTTCAACTTAACTTGACCGCTCT32960.10060736814665722No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGTTG506.366463E-11116.7245944
ACGAACA1000.0100.04964444
CAGTACG252.23005E-590.4080743
CCTTAGC65350.077.8187343
CGTGCGC2250.074.1108544
GAACACA31400.069.301944
TCGTGCG3500.067.8060543
TGAACAC23250.065.132743
CCGGATA351.181842E-464.57719443
ATAGCGG351.949702E-763.78658342
TAATCGT3200.063.5681843
ACCTTAG59750.060.84190442
TTAATCG2550.058.36680642
CTTAGCC18850.057.499844
AACCTTA85550.056.32798841
CTATCAT21900.055.96384444
TCGACCG200.005457756655.8132642
GATAGGC450.002440865255.58313844
AACGACA4150.054.46269643
ACTTCTA18400.053.4685644
Produced by FastQC (version 0.11.5)