##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR841036.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3276102
Sequences flagged as poor quality	0
Sequence length	24-50
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.65039092189437	39.0	37.0	39.0	32.0	39.0
2	37.304758826190394	39.0	37.0	39.0	34.0	39.0
3	37.515863974931186	39.0	37.0	39.0	35.0	39.0
4	37.89229150984921	39.0	37.0	39.0	35.0	41.0
5	38.720158590910785	40.0	38.0	41.0	35.0	41.0
6	38.700410732022384	40.0	38.0	41.0	35.0	41.0
7	38.74955572201354	40.0	38.0	41.0	35.0	41.0
8	38.688515803232015	40.0	38.0	41.0	35.0	41.0
9	38.74114175932251	40.0	38.0	41.0	35.0	41.0
10	38.718535320328854	40.0	38.0	41.0	35.0	41.0
11	38.65872308005062	40.0	38.0	41.0	35.0	41.0
12	38.610209328036795	40.0	38.0	41.0	35.0	41.0
13	38.53173741232721	40.0	37.0	41.0	35.0	41.0
14	38.534043811822706	40.0	37.0	41.0	35.0	41.0
15	38.49057019592186	40.0	37.0	41.0	35.0	41.0
16	38.37281165238445	40.0	37.0	41.0	34.0	41.0
17	38.34368649083576	40.0	37.0	41.0	34.0	41.0
18	38.314128803071455	40.0	36.0	41.0	34.0	41.0
19	38.30418283679812	40.0	36.0	41.0	34.0	41.0
20	38.25457601747443	40.0	36.0	41.0	34.0	41.0
21	38.16914125384375	40.0	36.0	41.0	34.0	41.0
22	38.085860574548654	40.0	36.0	41.0	34.0	41.0
23	38.01962789925344	40.0	36.0	41.0	34.0	41.0
24	37.88386777945253	39.0	35.0	41.0	34.0	41.0
25	37.79601437063695	39.0	35.0	41.0	33.0	41.0
26	37.66700305717794	39.0	35.0	41.0	33.0	41.0
27	37.53719173344874	39.0	35.0	41.0	33.0	41.0
28	37.42378726807923	39.0	35.0	41.0	33.0	41.0
29	37.38750599280737	38.0	35.0	40.0	33.0	41.0
30	37.37305152639058	38.0	35.0	40.0	33.0	41.0
31	37.31151251312692	38.0	35.0	40.0	33.0	41.0
32	37.27505461117233	38.0	35.0	40.0	33.0	41.0
33	37.1822542449675	38.0	35.0	40.0	33.0	41.0
34	37.09808621508872	38.0	35.0	40.0	33.0	41.0
35	36.9960915201466	38.0	35.0	40.0	32.0	41.0
36	36.9359696591277	38.0	35.0	40.0	32.0	41.0
37	36.85067729163068	38.0	35.0	40.0	32.0	41.0
38	36.72242503320395	38.0	35.0	40.0	32.0	41.0
39	36.638564407483656	38.0	35.0	40.0	31.0	41.0
40	36.47574877169499	37.0	34.0	40.0	31.0	41.0
41	36.35463833761478	37.0	34.0	40.0	31.0	41.0
42	36.194688848847804	37.0	34.0	40.0	31.0	41.0
43	36.03555044993832	36.0	34.0	40.0	31.0	41.0
44	35.825833253961484	36.0	34.0	40.0	30.0	41.0
45	35.6234561765685	36.0	34.0	40.0	29.0	41.0
46	35.2414017313111	36.0	33.0	39.0	28.0	41.0
47	35.116985782765006	35.0	33.0	39.0	29.0	41.0
48	35.96783427371587	37.0	34.0	39.0	31.0	41.0
49	36.89170234225187	37.0	35.0	40.0	33.0	41.0
50	36.56235931259446	38.0	35.0	40.0	32.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	3.0
20	18.0
21	41.0
22	110.0
23	253.0
24	528.0
25	1116.0
26	1980.0
27	3894.0
28	7703.0
29	14400.0
30	27001.0
31	51873.0
32	95485.0
33	171826.0
34	274978.0
35	195882.0
36	304319.0
37	505679.0
38	532701.0
39	483821.0
40	602491.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	48.96633255008544	17.093576451526847	33.94009099838772
2	23.092229729111	26.43467755277461	31.80621360384994	18.66687911426445
3	23.21872151721772	24.883230131418372	26.193750988217097	25.70429736314681
4	19.44182446089896	27.70936313948711	30.31499019261305	22.53382220700088
5	17.525858474491944	30.09262226878162	29.374207518569325	23.007311738157117
6	21.261242781818147	30.86317214787574	26.245825068938633	21.62976000136748
7	24.266552140317977	31.716625428634394	21.387124088322036	22.629698342725593
8	22.61611512706259	28.595080372955422	27.013719353060434	21.775085146921555
9	21.634124944827725	32.22860582484917	25.223054715634618	20.914214514688492
10	25.503479439895337	26.63531233154523	26.34017500065627	21.52103322790316
11	22.889580361051028	28.293227744435306	25.526860885283792	23.290331009229874
12	21.770445486740034	28.08282526001938	27.309925026754357	22.836804226486233
13	20.541942833281748	28.556925272778443	26.518649297244103	24.382482596695706
14	17.659126608390093	29.654388050188913	26.975564252883455	25.710921088537535
15	18.8614396010869	33.90010445340225	26.438065725670324	20.80039021984053
16	19.819376808170198	37.8376802675863	22.41077963995016	19.932163284293345
17	20.868367346315836	37.93169443442237	20.7597016210118	20.440236598249996
18	20.68232307785289	31.109745667259443	23.50238179397345	24.70554946091422
19	19.477659730985177	28.893850069381234	23.962410205787243	27.666079993846342
20	20.96778427533697	28.568890712193944	26.221863666027495	24.241461346441596
21	20.625639861029967	33.913779241305676	24.14091502645522	21.319665871209136
22	23.130690070089393	29.68552261193333	22.65475861252183	24.529028705455445
23	24.06896976956151	28.4874524663762	25.346738288368307	22.09683947569398
24	22.079776514894835	30.684758899448184	23.979045829464408	23.256418756192573
25	22.007155850318963	29.942438870461057	24.627795714470768	23.422609564749212
26	23.37872854274518	33.442325506949565	23.459901035683146	19.71904491462211
27	23.373825201201846	28.98775101032619	24.473430964751447	23.16499282372052
28	21.11067894167281	29.418798324994906	25.410329198099152	24.060193535233136
29	21.614395893393176	31.08110244886032	25.503332570133242	21.80116908761326
30	23.364454134397786	29.840523410345533	26.5753870514283	20.21963540382838
31	21.504661841243603	31.984251225740667	24.102420294370273	22.408666638645457
32	20.341256851425314	30.366589201262357	26.32668655189385	22.965467395418475
33	20.36310308895694	27.483779709572133	30.191809281496873	21.96130791997405
34	20.204476042395804	31.316872508671246	26.580382299482963	21.898269149449987
35	20.462714324728193	28.893666540579606	26.40088916027002	24.242729974422183
36	20.75630835100795	30.17440054973009	24.743040826709482	24.32625027255248
37	20.159507327637748	33.42322920957177	23.681689542006033	22.73557392078445
38	23.224431960458425	29.170358776225108	24.31273342089782	23.29247584241865
39	20.214218726862896	34.24575016008147	25.22803425323798	20.31199685981765
40	21.083004179701824	30.4329954755108	24.35212931554636	24.13187102924101
41	20.55819893486349	33.531777232084536	23.818964314549895	22.09105951850208
42	19.973120296998687	34.07501530228994	23.851846292714733	22.100018107996643
43	21.091610962171504	28.342808953817745	25.638830306492427	24.926749777518324
44	21.32336304850228	29.831364616347155	23.17687577520334	25.668396559947226
45	20.847717718594314	28.736242073731066	29.15850838158715	21.25753182608747
46	19.807989258140317	31.506322032001787	26.77416664123613	21.911522068621768
47	21.191568876161423	32.39873040947504	24.498466997649913	21.911233716713614
48	23.134316525741415	29.87454896746676	24.189443856784003	22.801690650007814
49	18.280646013063972	30.62051710480181	24.486336503374208	26.612500378760007
50	20.79019132847302	33.17298648249174	25.17240805650147	20.864414132533774
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	100144.0
1	89405.0
2	59125.0
3	37653.5
4	32413.5
5	23058.0
6	13529.5
7	9843.0
8	9202.0
9	8550.0
10	8007.5
11	7438.0
12	7087.5
13	7390.5
14	8415.5
15	9073.0
16	9008.5
17	9581.5
18	11650.0
19	13754.5
20	15303.0
21	18484.5
22	22689.0
23	28117.5
24	33097.0
25	36193.5
26	41291.0
27	50358.0
28	59313.5
29	68297.0
30	80279.5
31	96960.0
32	116883.5
33	128364.5
34	145044.0
35	169310.0
36	174197.5
37	169976.0
38	172245.0
39	182465.0
40	197358.5
41	209951.5
42	216251.5
43	212940.5
44	210560.0
45	214749.0
46	219238.5
47	214161.0
48	223798.0
49	244924.5
50	272704.0
51	293750.0
52	275103.0
53	244051.0
54	219164.0
55	198460.5
56	177218.5
57	157680.5
58	143364.5
59	126126.0
60	108396.5
61	96807.0
62	87033.0
63	74150.0
64	58851.5
65	46281.5
66	34361.0
67	24458.0
68	17829.0
69	12774.0
70	9396.5
71	6516.0
72	4157.5
73	2684.5
74	1938.0
75	1439.5
76	1111.5
77	749.5
78	395.0
79	303.0
80	269.0
81	240.5
82	203.5
83	152.5
84	121.5
85	71.0
86	29.0
87	22.0
88	18.0
89	15.0
90	7.0
91	1.5
92	2.0
93	1.5
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
24	18348.0
25	21436.0
26	22257.0
27	26011.0
28	25926.0
29	22102.0
30	23335.0
31	20251.0
32	19734.0
33	16588.0
34	17243.0
35	15931.0
36	16420.0
37	15394.0
38	16955.0
39	19826.0
40	26702.0
41	26849.0
42	30400.0
43	50135.0
44	117910.0
45	248674.0
46	401579.0
47	290148.0
48	610863.0
49	373654.0
50	781431.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	14.286621331959312
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.88787505629962	10.698947132844339
2	11.032382785278184	3.15230950484992
3	3.709019661765911	1.589680782613241
4	1.6904515565947145	0.9660336507635949
5	0.9818751838049835	0.7013839473134875
6	0.6185764160744543	0.5302420212801743
7	0.45351338405542296	0.4535421790882683
8	0.35494080970585584	0.4056723954821273
9	0.26045537170222355	0.33489245424459413
>10	2.9112313471102382	9.90407529850973
>50	1.5279024773000467	16.027636804917282
>100	1.4872305446684533	34.737557268043396
>500	0.04931815329000524	4.755523494877623
>1k	0.03010328837182138	8.138423742017862
>5k	0.003842972983636772	3.620127390886059
>10k+	0.0012809909945455907	3.983951932268268
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGC	36000	1.098866885096984	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTA	24395	0.7446349350539146	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCT	14184	0.4329535527282118	No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACACA	12497	0.38145942952936146	No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAGAA	8563	0.26137769825237434	No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACA	8036	0.24529150801776015	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAA	7821	0.23872883078731982	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAA	7664	0.2339365502050913	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAA	6990	0.21336332018966442	No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATCAT	6469	0.19746027443589975	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAA	6104	0.18631898518422196	No Hit
CAGAGTGTTGCTTAACACAAAGCACCCAACTTACACTTAGGAGATTTCAA	5781	0.1764597072984907	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGCC	5660	0.17276629360135917	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA	5658	0.17270524544107602	No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCTA	5246	0.16012932442274386	No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAG	5132	0.15664957928660342	No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	4795	0.14636296427888998	No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAA	4492	0.13711416799599035	No Hit
CACTTAGGAGATTTCAACTTAACTTGACCGCTCTGACCAAAAAAAAAAAA	4473	0.13653421047330028	No Hit
AAAAAAAAAAAAAAAAAAAAAAAA	4324	0.13198612253220443	No Hit
AACACAAAGCACCCAACTTACACTTAGGAGATTTCAACTTAACTTGACCG	4273	0.13042939444498372	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	4000	0.12209632056633156	No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATC	3936	0.12014277943727028	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAG	3749	0.11443477645079426	No Hit
CAGAGTGTTGCTTAACACAAAGCACCCAACTTACACTTAGGAGATTTC	3737	0.11406848748909527	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACC	3614	0.11031402563168058	No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCC	3469	0.10588803401115107	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTT	3409	0.10405658920265609	No Hit
CAAAGCACCCAACTTACACTTAGGAGATTTCAACTTAACTTGACCGCTCT	3296	0.10060736814665722	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.07182316057314454	0.0	0.0	0.0	0.0
2	0.12795694395351548	0.0	0.0	0.0	0.0
3	0.16244915451350417	0.0	0.0	0.0	0.0
4	0.19010397112177826	0.0	0.0	0.0	0.0
5	0.21611048740240688	0.0	0.0	0.0	0.0
6	0.2599430664857199	0.0	0.0	0.0	0.0
7	0.29345850648117794	0.0	0.0	0.0	0.0
8	0.3274318076787597	0.0	0.0	0.0	0.0
9	0.3611303921550672	0.0	0.0	0.0	0.0
10	0.3928449114221718	0.0	0.0	0.0	0.0
11	0.4243152380481438	0.0	0.0	0.0	0.0
12	0.4546561737088772	0.0	0.0	0.0	0.0
13	0.4876832284220699	0.0	0.0	0.0	0.0
14	0.5193061754487498	0.0	0.0	0.0	0.0
15	0.5492808221477842	0.0	0.0	0.0	3.052408014158289E-5
16	0.5786144631638453	0.0	0.0	0.0	3.052408014158289E-5
17	0.6091690673855698	0.0	0.0	0.0	3.052408014158289E-5
18	0.6355113485477558	0.0	0.0	0.0	3.052408014158289E-5
19	0.6609684313858359	0.0	0.0	0.0	3.052408014158289E-5
20	0.6885621998338269	0.0	0.0	0.0	3.052408014158289E-5
21	0.7134393251492169	0.0	0.0	0.0	3.052408014158289E-5
22	0.7375533484610675	0.0	0.0	0.0	3.052408014158289E-5
23	0.7630409553792892	0.0	0.0	0.0	3.052408014158289E-5
24	0.7860866358861842	0.0	0.0	0.0	3.052408014158289E-5
25	0.8116658150448307	0.0	0.0	0.0	3.052408014158289E-5
26	0.8399921614162197	0.0	0.0	0.0	3.052408014158289E-5
27	0.8640451365677869	0.0	0.0	0.0	3.052408014158289E-5
28	0.8905400381306809	0.0	0.0	0.0	3.052408014158289E-5
29	0.9188969085822114	0.0	0.0	0.0	3.052408014158289E-5
30	0.9477726883961488	0.0	0.0	0.0	3.052408014158289E-5
31	0.9764347996490952	0.0	0.0	0.0	3.052408014158289E-5
32	1.0053105794630326	0.0	0.0	3.052408014158289E-5	3.052408014158289E-5
33	1.0325991071096077	0.0	0.0	3.052408014158289E-5	3.052408014158289E-5
34	1.0598571106760413	0.0	0.0	3.052408014158289E-5	3.052408014158289E-5
35	1.0840016580680334	0.0	0.0	3.052408014158289E-5	3.052408014158289E-5
36	1.1057348031288403	0.0	0.0	3.052408014158289E-5	3.052408014158289E-5
37	1.1261859368237008	0.0	0.0	3.052408014158289E-5	3.052408014158289E-5
38	1.1390670986434488	0.0	0.0	3.052408014158289E-5	3.052408014158289E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCCGTTG	50	6.366463E-11	116.72459	44
ACGAACA	100	0.0	100.049644	44
CAGTACG	25	2.23005E-5	90.40807	43
CCTTAGC	6535	0.0	77.81873	43
CGTGCGC	225	0.0	74.11085	44
GAACACA	3140	0.0	69.3019	44
TCGTGCG	350	0.0	67.80605	43
TGAACAC	2325	0.0	65.1327	43
CCGGATA	35	1.181842E-4	64.577194	43
ATAGCGG	35	1.949702E-7	63.786583	42
TAATCGT	320	0.0	63.56818	43
ACCTTAG	5975	0.0	60.841904	42
TTAATCG	255	0.0	58.366806	42
CTTAGCC	1885	0.0	57.4998	44
AACCTTA	8555	0.0	56.327988	41
CTATCAT	2190	0.0	55.963844	44
TCGACCG	20	0.0054577566	55.81326	42
GATAGGC	45	0.0024408652	55.583138	44
AACGACA	415	0.0	54.462696	43
ACTTCTA	1840	0.0	53.46856	44
>>END_MODULE