FastQCFastQC Report
Tue 24 May 2016
ERR841035.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR841035.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1174414
Sequences flagged as poor quality0
Sequence length24-50
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAA89190.7594425815768545No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAA84310.7178899434100752No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAA80550.6858739762979664No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA68010.5790973200251359No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAA65990.5618972525872478No Hit
AAAAAAAAAAAAAAAAAAAAAAAA53800.4581008060190018No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGC47100.4010510773883826No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAA41640.3545598059968631No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTA30930.2633653890365748No Hit
AGCTTTAATTTATTAATGCAAACAGTACCTAACAAACCCACAGGTCCT24020.20452753458320488No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA23730.20205821797083479No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAGAA23680.2016324737273227No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA22150.188604699875853No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACACA20160.17166007898407207No Hit
CACTTAGGAGATTTCAACTTAACTTGACCGCTCTGACCAAAAAAAAAAAA19370.16493331993658114No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCT18250.15539664888191046No Hit
CGGCAAGTTTGAATTTCGTGGAGGCTCGGG16930.14415700085319147No Hit
TCAGTTCTTTGACCATTCGTTGGAGCTCCGGTTTTCAGCCTCTTTCCG16320.13896292108234404No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAA16190.13785598604921262No Hit
AACACAAAGCACCCAACTTACACTTAGGAGATTTCAACTTAACTTGACCG16160.13760053950310536No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAA15410.13121437585042411No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATCAT14900.12687178456660086No Hit
TAGATGTTCGCGATTGTCTCGGAAGCCCCCAGCACCTGCTTGTGTGGTGT14430.1228697886775873No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAA13960.11886779278857369No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAG13940.11869749509116888No Hit
AGCTTTAATTTATTAATGCAAACAGTACCTAACAAACCCACAGGTC13660.1163133273275012No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA12890.10975686597741513No Hit
AGGTTTTCTGTTTCCTGTGATACCTTGGGCTGGAATCTGAAGCAATGAA12850.10941627058260546No Hit
CTCTTTTCCTCCTTGGCTGTCTGAAGATAGATCGCCATCATGAACGACA12790.10890537749039095No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAA12720.10830933554947404No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACA12670.10788359130596195No Hit
CACTTAGGAGATTTCAACTTAACTTGACCGCTCTGACCAAAAAAAAAA12650.10771329360855712No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA11850.10090138571236379No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTT11820.10064593916625654No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAAATC153.8556813E-5193.9398344
GCGAACC256.51562E-9193.9398344
GCGCGCA256.51562E-9193.9398344
ACCGCGA256.51562E-9193.9398344
CGACGTT204.998292E-7193.9398244
CCCGTAC100.0029697758193.9398244
TATACGA204.998292E-7193.9398244
TAACCGA201.2135769E-4145.4548644
CCCGAGA201.2135769E-4145.4548644
GCAACTA150.009984356129.2932344
TACGTAT150.009984356129.2932344
CTATCAT2350.0127.9177644
CGTACAC554.1836756E-11123.4162644
CCGACGT204.8035163E-6123.1201443
GTATAAC302.0281732E-9123.1201443
CGATCAT152.3581236E-4123.1201443
TAATCGT204.8035163E-6123.1201443
GCTCTAT401.0787153E-7121.2123944
CGGTCCT401.0787153E-7121.2123944
GTCATAG252.9506572E-4116.363944
Produced by FastQC (version 0.11.5)