##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR841035.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1174414
Sequences flagged as poor quality	0
Sequence length	24-50
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.01307971464918	39.0	35.0	39.0	30.0	39.0
2	36.93328417406468	39.0	37.0	39.0	33.0	39.0
3	37.252582990325386	39.0	37.0	39.0	33.0	39.0
4	37.68734619989203	39.0	36.0	39.0	34.0	41.0
5	38.50202058217971	40.0	36.0	41.0	35.0	41.0
6	38.491009984554	40.0	36.0	41.0	35.0	41.0
7	38.50144753042794	40.0	36.0	41.0	35.0	41.0
8	38.40400999988079	40.0	36.0	41.0	35.0	41.0
9	38.292541642044455	40.0	35.0	41.0	35.0	41.0
10	38.24813651744615	40.0	35.0	41.0	34.0	41.0
11	38.115587859136554	40.0	35.0	41.0	34.0	41.0
12	37.90508968728234	39.0	35.0	41.0	34.0	41.0
13	37.692289942047694	39.0	35.0	41.0	34.0	41.0
14	37.58966003470667	39.0	35.0	41.0	34.0	41.0
15	37.5021789590383	39.0	35.0	40.0	34.0	41.0
16	37.38898803999271	38.0	35.0	40.0	34.0	41.0
17	37.25028822885286	38.0	35.0	40.0	33.0	41.0
18	37.17670259380423	38.0	35.0	40.0	33.0	41.0
19	37.00168254125036	37.0	35.0	40.0	33.0	41.0
20	36.84699347930117	37.0	35.0	40.0	33.0	41.0
21	36.67123092878661	37.0	35.0	40.0	33.0	41.0
22	36.499000352516234	37.0	35.0	40.0	33.0	41.0
23	36.35697292436909	36.0	35.0	40.0	33.0	41.0
24	36.13520019345818	36.0	35.0	40.0	32.0	41.0
25	36.01924997612786	36.0	35.0	40.0	32.0	41.0
26	35.782654104707596	35.0	34.0	39.0	32.0	41.0
27	35.673876788013416	35.0	34.0	39.0	31.0	41.0
28	35.572648635039386	35.0	34.0	39.0	31.0	41.0
29	35.51358079089641	35.0	34.0	39.0	31.0	41.0
30	35.48503536639886	35.0	34.0	39.0	31.0	41.0
31	35.359938970901055	35.0	34.0	38.0	31.0	41.0
32	35.269696205782886	35.0	34.0	38.0	31.0	40.0
33	35.17610710607621	35.0	34.0	38.0	31.0	40.0
34	35.08639865364806	35.0	34.0	38.0	31.0	40.0
35	34.96653614460766	35.0	34.0	37.0	31.0	40.0
36	34.88285120398213	35.0	34.0	37.0	31.0	39.0
37	34.72618125331036	35.0	34.0	37.0	31.0	39.0
38	34.557670575502065	35.0	34.0	36.0	30.0	39.0
39	34.430228456560386	35.0	33.0	36.0	30.0	39.0
40	34.183340601566904	35.0	33.0	36.0	30.0	39.0
41	34.104033811781356	35.0	33.0	36.0	30.0	38.0
42	33.95711436719848	35.0	33.0	35.0	30.0	37.0
43	33.80865820522774	35.0	33.0	35.0	29.0	37.0
44	33.56356864591806	35.0	32.0	35.0	29.0	37.0
45	33.381805342637435	35.0	32.0	35.0	29.0	37.0
46	33.09937067330058	35.0	32.0	35.0	27.0	37.0
47	33.101071485926894	35.0	32.0	35.0	27.0	36.0
48	33.62061282323769	35.0	33.0	35.0	29.0	37.0
49	34.23716794880523	35.0	34.0	35.0	31.0	37.0
50	33.85767609100443	35.0	33.0	35.0	30.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	2.0
20	11.0
21	27.0
22	81.0
23	203.0
24	421.0
25	812.0
26	1335.0
27	2759.0
28	5216.0
29	9511.0
30	16842.0
31	29590.0
32	52535.0
33	94744.0
34	159829.0
35	152796.0
36	205295.0
37	182845.0
38	124501.0
39	102628.0
40	32431.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	49.66894127624501	21.275291336785834	29.055767386969162
2	23.710889005069763	27.508527657197547	29.431529256292926	19.349054081439764
3	21.54351021019845	27.47080671722238	25.749182145308215	25.23650092727096
4	17.992973517005076	29.824661490751986	30.656054849482377	21.52631014276056
5	16.866879992915614	32.63006060895051	28.730839380320738	21.77222001781314
6	21.244637751252966	35.070511761610476	26.11217168732661	17.57267879980995
7	26.58440720223022	32.64445076438122	20.632076933687777	20.139065099700787
8	23.85981434145029	30.105141798377744	25.952687893706987	20.082355966464977
9	22.344249983395976	32.85596050455802	24.52440110557265	20.275388406473354
10	23.39694519990395	28.982539377085082	26.573678447293712	21.04683697571725
11	23.964888020749072	31.45798670656174	24.245879221467046	20.33124605122214
12	22.95604446132284	31.07583867358529	26.22610084689045	19.74201601820142
13	21.071274695294846	32.819857392708194	24.19598199612743	21.91288591586953
14	18.785539000727173	32.2562571631469	26.92525804358599	22.03294579253994
15	20.58626685308588	34.36897039715126	25.319691352453223	19.725071397309637
16	21.890321470963393	38.32847701066234	20.992597159093812	18.78860435928046
17	21.85149359595509	36.06249584899363	22.044526035963468	20.041484519087817
18	22.01089224072601	33.33134652686361	20.250610091500953	24.407151140909424
19	21.282018095833326	31.166947941696883	23.883315423692157	23.66771853877764
20	23.049878492592903	31.615341778963806	23.39430558559418	21.940474142849116
21	23.140562016460976	33.57776729500841	22.240623834525135	21.041046854005486
22	23.1449897565935	34.5781811184131	21.10209857852512	21.174730546468282
23	24.24289901176246	32.3550298276417	22.050060711129124	21.352010449466714
24	22.60633813970201	33.25650068885418	23.718382103755577	20.418779067688224
25	24.257103359046422	30.873943926712965	24.554924413498124	20.314028300742486
26	23.0556177919047	33.86541779382265	22.696351806922213	20.38261260735044
27	23.266635068407872	32.912357277267645	23.80445393964261	20.016553714681873
28	22.950221199151173	30.576916160126604	23.10470093155415	23.368161709168074
29	24.078570396874298	29.836419849017005	23.866759057974328	22.218250696134376
30	27.604527047457573	29.128723303609355	24.616608943905348	18.650140705027724
31	22.328800388538124	32.016652171785054	24.35375417433766	21.30079326533916
32	23.499507707821024	29.64160178081345	25.321131463415796	21.537759047949734
33	21.75455401422233	28.27582367693877	27.79534746223904	22.174274846599857
34	22.977062689512092	30.334024886232292	24.882556908033138	21.806355516222478
35	22.788294129625065	27.871249845331846	25.12483059574763	24.215625429295464
36	22.80853268848778	29.539075442219065	25.433877160062497	22.21851470923066
37	22.293414073061022	31.7749630426018	24.001754982331597	21.929867902005583
38	23.6245130535472	30.154569437867824	24.486691788262352	21.734225720322627
39	23.366843234988185	31.085243818605612	23.974711247662626	21.57320169874358
40	20.68684301323763	30.995660308058433	25.876968873410306	22.440527805293637
41	21.291277902082456	31.953994543320068	24.621120705523357	22.133606849074123
42	23.433185913862943	33.144167975531126	23.038046623129702	20.384599487476233
43	23.152334149763494	28.657430996655116	25.072217973251366	23.118016880330032
44	23.026135536623997	29.916734449248683	23.699109550922557	23.35802046320476
45	23.11495548178154	28.382578052117847	27.633783084092222	20.868683382008392
46	21.319868241383592	31.213551025116644	25.173820310237122	22.292760423262642
47	23.036121938370425	31.834793950209452	23.44136244861325	21.687721662806876
48	23.25363088912504	31.74442082890542	23.653382925965285	21.34856535600425
49	21.20334944516305	30.827421880318607	24.100483354891416	23.86874531962693
50	21.056010958200456	31.865248378742805	24.415812340817155	22.66292832223958
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	82704.0
1	78550.0
2	51342.5
3	26913.5
4	23643.5
5	16719.0
6	8171.0
7	4602.5
8	4240.5
9	3632.0
10	3022.0
11	2792.5
12	3025.5
13	3186.0
14	3339.5
15	3742.5
16	3961.5
17	3712.5
18	3508.5
19	3747.0
20	4136.0
21	4802.5
22	5770.0
23	6780.5
24	7588.0
25	8240.0
26	9793.5
27	11914.0
28	13677.0
29	16645.5
30	20843.5
31	24458.0
32	28182.0
33	34264.5
34	44854.5
35	51465.5
36	49781.5
37	48581.5
38	49100.5
39	51375.5
40	56506.5
41	65258.0
42	73602.5
43	74655.5
44	72719.5
45	71748.5
46	75005.0
47	80065.5
48	83650.5
49	90530.0
50	98978.0
51	100634.0
52	97536.0
53	91581.0
54	82998.0
55	79127.5
56	77056.5
57	71115.0
58	63205.5
59	55216.0
60	48629.5
61	42597.0
62	35628.0
63	28781.5
64	22054.0
65	16306.0
66	10670.0
67	6466.5
68	4642.0
69	3539.5
70	2763.0
71	2132.5
72	1602.0
73	1182.0
74	804.0
75	514.0
76	353.0
77	253.5
78	144.5
79	74.5
80	36.0
81	26.0
82	17.0
83	8.5
84	7.0
85	3.5
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
24	11971.0
25	15382.0
26	16197.0
27	18744.0
28	18222.0
29	15756.0
30	15698.0
31	11239.0
32	9322.0
33	7978.0
34	7514.0
35	7439.0
36	6976.0
37	6732.0
38	7330.0
39	9595.0
40	10435.0
41	10124.0
42	11973.0
43	21427.0
44	47869.0
45	88972.0
46	131248.0
47	101671.0
48	197375.0
49	133610.0
50	233615.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	13.119439502077382
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.52306937196859	8.989842631221935
2	11.333049254195918	2.9736650812897305
3	4.463617410813273	1.7568047574475207
4	2.283077355412649	1.1981078097159628
5	1.4683793503367772	0.9632157026421518
6	1.0121698149252238	0.7967460391644201
7	0.7680594725050955	0.7053556848469646
8	0.6155373273996692	0.6460403782472286
9	0.5248623162666904	0.6197309464662946
>10	5.205204298617785	16.046225191890994
>50	2.2368080260353516	21.10611850158509
>100	1.4960989243351008	31.145103289363462
>500	0.042707551809744185	3.9338252590392737
>1k	0.02335569239595385	5.250965977031126
>5k	0.004003832982163517	3.8682527500478554
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAA	8919	0.7594425815768545	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAA	8431	0.7178899434100752	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAA	8055	0.6858739762979664	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA	6801	0.5790973200251359	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAA	6599	0.5618972525872478	No Hit
AAAAAAAAAAAAAAAAAAAAAAAA	5380	0.4581008060190018	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGC	4710	0.4010510773883826	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	4164	0.3545598059968631	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTA	3093	0.2633653890365748	No Hit
AGCTTTAATTTATTAATGCAAACAGTACCTAACAAACCCACAGGTCCT	2402	0.20452753458320488	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2373	0.20205821797083479	No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAGAA	2368	0.2016324737273227	No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2215	0.188604699875853	No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACACA	2016	0.17166007898407207	No Hit
CACTTAGGAGATTTCAACTTAACTTGACCGCTCTGACCAAAAAAAAAAAA	1937	0.16493331993658114	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCT	1825	0.15539664888191046	No Hit
CGGCAAGTTTGAATTTCGTGGAGGCTCGGG	1693	0.14415700085319147	No Hit
TCAGTTCTTTGACCATTCGTTGGAGCTCCGGTTTTCAGCCTCTTTCCG	1632	0.13896292108234404	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAA	1619	0.13785598604921262	No Hit
AACACAAAGCACCCAACTTACACTTAGGAGATTTCAACTTAACTTGACCG	1616	0.13760053950310536	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1541	0.13121437585042411	No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATCAT	1490	0.12687178456660086	No Hit
TAGATGTTCGCGATTGTCTCGGAAGCCCCCAGCACCTGCTTGTGTGGTGT	1443	0.1228697886775873	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAA	1396	0.11886779278857369	No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAG	1394	0.11869749509116888	No Hit
AGCTTTAATTTATTAATGCAAACAGTACCTAACAAACCCACAGGTC	1366	0.1163133273275012	No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1289	0.10975686597741513	No Hit
AGGTTTTCTGTTTCCTGTGATACCTTGGGCTGGAATCTGAAGCAATGAA	1285	0.10941627058260546	No Hit
CTCTTTTCCTCCTTGGCTGTCTGAAGATAGATCGCCATCATGAACGACA	1279	0.10890537749039095	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1272	0.10830933554947404	No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACA	1267	0.10788359130596195	No Hit
CACTTAGGAGATTTCAACTTAACTTGACCGCTCTGACCAAAAAAAAAA	1265	0.10771329360855712	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1185	0.10090138571236379	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTT	1182	0.10064593916625654	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.08557459294592878	0.0	0.0	0.0	0.0
2	0.16569965957490287	0.0	0.0	0.0	0.0
3	0.23484052472126524	0.0	0.0	0.0	0.0
4	0.2933377837798255	0.0	0.0	0.0	0.0
5	0.3463003676727287	0.0	0.0	0.0	0.0
6	0.4158669770626031	0.0	0.0	0.0	0.0
7	0.46644539319183864	0.0	0.0	0.0	0.0
8	0.5191525305386346	0.0	0.0	0.0	0.0
9	0.5717745190367282	0.0	0.0	0.0	0.0
10	0.6313787131284198	0.0	0.0	0.0	0.0
11	0.6934522238324815	0.0	0.0	0.0	0.0
12	0.7530564179241732	0.0	0.0	0.0	0.0
13	0.812064570074948	0.0	0.0	0.0	0.0
14	0.8632390281451005	0.0	0.0	0.0	0.0
15	0.9104114903262392	0.0	0.0	0.0	0.0
16	0.9463443044786591	0.0	0.0	0.0	0.0
17	0.9803186951109234	0.0	0.0	0.0	0.0
18	1.0134415972561635	0.0	0.0	0.0	0.0
19	1.043413991999414	0.0	0.0	0.0	0.0
20	1.0667447765438764	0.0	0.0	0.0	0.0
21	1.09118249612147	0.0	0.0	0.0	0.0
22	1.117748936916624	0.0	0.0	0.0	0.0
23	1.1421015076455152	0.0	0.0	0.0	0.0
24	1.1691788415328837	0.0	0.0	0.0	0.0
25	1.1926799237747507	0.0	0.0	0.0	0.0
26	1.2191612157212022	0.0	0.0	0.0	0.0
27	1.2480266754313214	0.0	0.0	0.0	0.0
28	1.2748485627725827	0.0	0.0	0.0	0.0
29	1.3063536367924768	0.0	0.0	0.0	0.0
30	1.33394186377206	0.0	0.0	0.0	0.0
31	1.3648508958510372	0.0	0.0	0.0	0.0
32	1.390736145856572	0.0	0.0	0.0	0.0
33	1.4178134797439403	0.0	0.0	0.0	0.0
34	1.4403779246500807	0.0	0.0	0.0	0.0
35	1.4660077281095083	0.0	0.0	0.0	0.0
36	1.4857622610084689	0.0	0.0	0.0	0.0
37	1.506197984697049	0.0	0.0	0.0	0.0
38	1.5206732889764598	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGAAATC	15	3.8556813E-5	193.93983	44
GCGAACC	25	6.51562E-9	193.93983	44
GCGCGCA	25	6.51562E-9	193.93983	44
ACCGCGA	25	6.51562E-9	193.93983	44
CGACGTT	20	4.998292E-7	193.93982	44
CCCGTAC	10	0.0029697758	193.93982	44
TATACGA	20	4.998292E-7	193.93982	44
TAACCGA	20	1.2135769E-4	145.45486	44
CCCGAGA	20	1.2135769E-4	145.45486	44
GCAACTA	15	0.009984356	129.29323	44
TACGTAT	15	0.009984356	129.29323	44
CTATCAT	235	0.0	127.91776	44
CGTACAC	55	4.1836756E-11	123.41626	44
CCGACGT	20	4.8035163E-6	123.12014	43
GTATAAC	30	2.0281732E-9	123.12014	43
CGATCAT	15	2.3581236E-4	123.12014	43
TAATCGT	20	4.8035163E-6	123.12014	43
GCTCTAT	40	1.0787153E-7	121.21239	44
CGGTCCT	40	1.0787153E-7	121.21239	44
GTCATAG	25	2.9506572E-4	116.3639	44
>>END_MODULE