FastQCFastQC Report
Tue 24 May 2016
ERR841034.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR841034.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2995385
Sequences flagged as poor quality0
Sequence length24-50
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGC501081.6728400522804248No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTA330351.1028632379477095No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCT185650.6197867719842358No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATCAT110250.368066208517436No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAA66290.22130711077207105No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAA63380.21159216594861763No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAA61490.20528245951689014No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA50650.1690934554322733No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAA49130.16401898253479938No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACC49030.16368513563364975No Hit
AGTGCAGTGCTGCTGTGGGGAAAAAAAAAAAAAAAAAAAAAAAAA41300.13787877017478553No Hit
AAAAAAAAAAAAAAAAAAAAAAAA41170.13744476920329105No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTA38850.1296995210966203No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA36590.1221545811306393No Hit
TCAGTTCTTTGACCATTCGTTGGAGCTCCGGTTTTCAGCCTCTTTCCG35930.11995119158305194No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCTA34890.11647918381109607No Hit
AGTGCAGTGCTGCTGTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA32110.10719823995913715No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAA31770.10606316049522849No Hit
CCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCTAG30500.10182330485062854No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGCGG100.0024417376207.0779744
CGCGTAC850.0133.9916244
TACGACT152.7765802E-4118.1843543
CTATCAT22500.0113.6627844
CGAATGG1450.0105.95837443
GATCTAT554.878839E-794.1263444
AATCGCA3050.081.4732944
AAATCCG204.124945E-579.8679542
GCGTATA304.1078238E-879.8679542
GTATCCG251.2976761E-679.8679442
CGCAAAT454.512367E-878.7895643
CCTTAGC83100.077.2962643
GCGAAGT951.877197E-976.29188544
CTACGTC951.877197E-976.29188544
CAACGAC555.52013E-575.3010844
CTTGCGA552.1045707E-975.2082243
CGTACCA2350.072.9222643
GCGCGTA900.072.2237643
TTACGTT3450.072.02711544
CCCTATC22050.069.3637742
Produced by FastQC (version 0.11.5)