##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR841034.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2995385
Sequences flagged as poor quality	0
Sequence length	24-50
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.24257549530361	39.0	35.0	39.0	31.0	39.0
2	37.125347159046335	39.0	37.0	39.0	33.0	39.0
3	37.230877833734226	39.0	37.0	39.0	33.0	39.0
4	37.818185976093226	39.0	37.0	39.0	34.0	41.0
5	38.51626785872267	40.0	37.0	41.0	34.0	41.0
6	38.478793210221724	40.0	37.0	41.0	35.0	41.0
7	38.473487381421755	40.0	37.0	41.0	35.0	41.0
8	38.450333429592526	40.0	37.0	41.0	35.0	41.0
9	38.51527599957935	40.0	37.0	41.0	35.0	41.0
10	38.483057102843205	40.0	37.0	41.0	35.0	41.0
11	38.44189010761555	40.0	37.0	41.0	34.0	41.0
12	38.351536446900816	40.0	37.0	41.0	34.0	41.0
13	38.216934050213915	40.0	36.0	41.0	34.0	41.0
14	38.193045635202154	40.0	36.0	41.0	34.0	41.0
15	38.10734980645226	40.0	36.0	41.0	34.0	41.0
16	38.007029146503704	40.0	36.0	41.0	34.0	41.0
17	38.03620436104207	40.0	36.0	41.0	34.0	41.0
18	38.040695603403236	40.0	35.0	41.0	34.0	41.0
19	38.05386719904119	40.0	36.0	41.0	34.0	41.0
20	37.95671841850046	40.0	35.0	41.0	34.0	41.0
21	37.79931327692434	39.0	35.0	41.0	33.0	41.0
22	37.75651210111555	39.0	35.0	41.0	33.0	41.0
23	37.67358019086027	39.0	35.0	41.0	33.0	41.0
24	37.5067535558868	39.0	35.0	41.0	33.0	41.0
25	37.43768520823444	39.0	35.0	41.0	33.0	41.0
26	37.28831685098402	38.0	35.0	40.0	33.0	41.0
27	37.18377858220787	38.0	35.0	40.0	33.0	41.0
28	37.045143555515324	38.0	35.0	40.0	33.0	41.0
29	36.97374295076043	38.0	35.0	40.0	32.0	41.0
30	36.878627788124106	38.0	35.0	40.0	32.0	41.0
31	36.79170832238988	38.0	35.0	40.0	32.0	41.0
32	36.684949680236116	38.0	35.0	40.0	32.0	41.0
33	36.57412156434753	37.0	35.0	40.0	32.0	41.0
34	36.49964996986854	37.0	35.0	40.0	32.0	41.0
35	36.347490083659636	37.0	34.0	40.0	31.0	41.0
36	36.28461075551579	37.0	34.0	40.0	31.0	41.0
37	36.24529827893114	37.0	34.0	40.0	31.0	41.0
38	36.05418892807317	36.0	34.0	40.0	31.0	41.0
39	35.98969369541718	36.0	34.0	40.0	31.0	41.0
40	35.7645539078655	36.0	34.0	39.0	31.0	41.0
41	35.64877492334939	36.0	34.0	39.0	30.0	41.0
42	35.51970357085126	35.0	34.0	39.0	30.0	41.0
43	35.33769968873197	35.0	34.0	39.0	30.0	41.0
44	35.072915467881586	35.0	33.0	39.0	29.0	41.0
45	34.84256398843763	35.0	33.0	39.0	29.0	41.0
46	34.53739704313295	35.0	32.0	39.0	28.0	41.0
47	34.46680664959812	35.0	33.0	38.0	28.0	40.0
48	35.36869866279448	36.0	34.0	39.0	30.0	40.0
49	35.912592224454336	36.0	34.0	39.0	31.0	41.0
50	36.1044733851936	37.0	34.0	39.0	31.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	8.0
21	38.0
22	82.0
23	209.0
24	569.0
25	1158.0
26	2262.0
27	4391.0
28	8809.0
29	17249.0
30	32624.0
31	61448.0
32	110829.0
33	184846.0
34	279323.0
35	210628.0
36	341109.0
37	496935.0
38	447279.0
39	411973.0
40	383616.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	46.928959048669874	18.827963684134094	34.243077267196036
2	25.037048659855078	21.212131328693975	34.88853686587868	18.862283145572274
3	22.120295053891237	24.6648761344535	29.5814728323738	23.63335597928146
4	20.63314064803022	25.841452768175046	30.510435219512683	23.014971364282054
5	18.11747070910751	27.250153152265906	31.69726095309952	22.93511518552707
6	21.370842145500497	28.063003587184955	26.65293443079938	23.91321983651517
7	25.727744513643486	25.056879165783364	24.188743684033938	25.026632636539208
8	24.227303001116717	25.78643479886559	29.8854070511804	20.100855148837297
9	25.807600692398474	28.223216715046647	25.051704538815546	20.917478053739337
10	28.846408725422606	22.71107053016557	25.46390530766496	22.978615436746864
11	25.270808260040027	23.691779186982643	26.86279059286202	24.17462196011531
12	24.855669638460498	25.821555492866526	25.00449858699299	24.318276281679985
13	22.86961442352152	24.18437028962888	25.001560734262874	27.944454552586727
14	19.71699798189548	25.38111127617986	27.988722651679165	26.91316809024549
15	22.971370958991916	32.575678919404346	23.788060633274185	20.664889488329546
16	20.8145196694248	33.62809789058836	24.131555709867012	21.425826730119834
17	23.075163960559326	34.25576344943972	21.303805687749655	21.365266902251296
18	22.951106452092134	27.669565014180147	22.53890568324272	26.840422850484995
19	24.03186902518374	26.229950407042836	22.613286772818853	27.124893794954573
20	22.747760304601915	26.542731568729895	24.045957364412253	26.663550762255937
21	22.324108587043067	33.81031152923581	23.686905022225858	20.178674861495267
22	23.243022182457345	29.104171917800215	21.860562164796846	25.79224373494559
23	23.336966700440843	28.802908474202816	27.342328281673307	20.51779654368303
24	22.26211321749959	30.192646354308376	23.71254446423415	23.83269596395789
25	22.49031399357077	29.744849779135873	23.362079767744127	24.402756459549227
26	23.23613592497843	34.96334917998888	23.35604345099478	18.44447144403791
27	24.105420215422317	30.191564581667546	22.69933523085446	23.003679972055682
28	23.52650857967098	29.333226869718477	22.110072134835253	25.030192415775293
29	22.062599679635298	30.598963852993606	26.97540280128007	20.36303366609103
30	24.09948683209977	30.33560991533833	26.78199838809728	18.78290486446462
31	23.45669075638321	33.38459105376271	23.60979318992468	19.548924999929397
32	21.302721194923624	29.98327180926305	24.78266159674174	23.93134539907159
33	22.229677299111664	28.487497513480964	29.58751561354745	19.69530957385992
34	22.955842075065224	32.359383513941665	24.57904365994392	20.10573075104919
35	20.524725517370722	28.778522767873856	24.741364614921054	25.955387099834365
36	20.268261952749057	29.281974843715798	25.93424606187236	24.51551714166278
37	21.755940504496575	35.67423606820015	22.42161157543996	20.148211851863316
38	25.907918550609484	28.601098965097304	23.283029324406883	22.207953159886326
39	20.77682701228514	34.01248653469705	23.698111177163593	21.512575275854225
40	20.92971440527646	30.009104498149885	24.099587752847746	24.96159334372591
41	21.087429300527507	34.06996629277675	23.869436879891833	20.973167526803906
42	20.828939367114916	35.00011546188603	22.504830155565646	21.666115015433405
43	21.209578821298468	28.342310619292384	24.110211649782922	26.33789890962623
44	21.042854417946952	28.441480505713752	23.873108839796387	26.642556236542912
45	21.19310130648692	28.133244649975254	29.914455764564963	20.759198278972864
46	21.37998120455707	29.026334817969197	27.64743705999703	21.9462469174767
47	20.400412143054673	33.593729091768736	25.61866062428076	20.387198140895833
48	26.266362887670404	27.617452482607657	23.657985847727616	22.45819878199432
49	19.454033226251674	28.66545114511511	23.963039362739373	27.917476265893843
50	21.12070623197273	29.065815925181365	26.928590158202955	22.88488768464295
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	81256.0
1	72835.5
2	51973.5
3	38149.5
4	34109.5
5	24096.5
6	12767.0
7	8671.0
8	8156.5
9	7728.5
10	7640.0
11	7285.5
12	6754.0
13	6152.5
14	5705.5
15	6107.5
16	7342.5
17	9020.5
18	11323.0
19	14471.5
20	17535.5
21	20406.5
22	22845.0
23	27560.0
24	33204.5
25	37173.5
26	43644.5
27	52821.0
28	60683.5
29	64893.5
30	67639.0
31	75227.0
32	88354.5
33	97431.0
34	104795.0
35	111701.0
36	114019.0
37	117552.0
38	120778.0
39	126034.5
40	135595.0
41	146726.0
42	157906.5
43	164621.0
44	169656.0
45	178282.5
46	192930.0
47	202545.0
48	224788.0
49	253479.0
50	284130.0
51	306872.0
52	276080.0
53	236869.5
54	223641.5
55	213849.5
56	204803.0
57	198789.5
58	190934.0
59	169687.0
60	141732.5
61	117197.0
62	94920.0
63	75363.0
64	58994.5
65	48234.0
66	35177.5
67	23467.5
68	17437.5
69	12945.0
70	9984.0
71	7687.5
72	5867.0
73	4197.0
74	2846.0
75	1804.0
76	1082.5
77	690.0
78	361.0
79	238.5
80	184.0
81	144.5
82	117.5
83	101.5
84	77.5
85	56.0
86	32.0
87	14.5
88	6.5
89	4.5
90	4.0
91	3.5
92	3.0
93	3.0
94	2.5
95	1.5
96	1.0
97	1.0
98	0.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
24	18668.0
25	21940.0
26	23233.0
27	26013.0
28	27561.0
29	22959.0
30	22267.0
31	21920.0
32	20779.0
33	18855.0
34	19923.0
35	19385.0
36	17929.0
37	18144.0
38	19486.0
39	23788.0
40	26985.0
41	27290.0
42	33268.0
43	53769.0
44	125206.0
45	263162.0
46	329303.0
47	317040.0
48	474473.0
49	429989.0
50	572050.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	14.679481429266552
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.2058911600934	10.89304001226766
2	10.906744993517977	3.2021072117218616
3	4.006734991659404	1.764503757061701
4	1.8791978168547256	1.1034259781774878
5	1.1161727301506352	0.8192418432049999
6	0.7147721173968635	0.6295490414090874
7	0.546385374796896	0.5614457773787715
8	0.37867327727271305	0.44469818731874367
9	0.3114311273559774	0.4114482705465856
>10	3.1179583093085546	10.497771954237425
>50	1.345349699198048	14.473914230560222
>100	1.3761114223565514	33.89901224600545
>500	0.05890607753248028	5.897999330389517
>1k	0.03305285461544727	8.824564557614577
>5k	0.0013090239451662284	1.161642707805797
>10k+	0.0013090239451662284	5.415634894300108
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGC	50108	1.6728400522804248	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTA	33035	1.1028632379477095	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCT	18565	0.6197867719842358	No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATCAT	11025	0.368066208517436	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAA	6629	0.22130711077207105	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAA	6338	0.21159216594861763	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAA	6149	0.20528245951689014	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA	5065	0.1690934554322733	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAA	4913	0.16401898253479938	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACC	4903	0.16368513563364975	No Hit
AGTGCAGTGCTGCTGTGGGGAAAAAAAAAAAAAAAAAAAAAAAAA	4130	0.13787877017478553	No Hit
AAAAAAAAAAAAAAAAAAAAAAAA	4117	0.13744476920329105	No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTA	3885	0.1296995210966203	No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3659	0.1221545811306393	No Hit
TCAGTTCTTTGACCATTCGTTGGAGCTCCGGTTTTCAGCCTCTTTCCG	3593	0.11995119158305194	No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCTA	3489	0.11647918381109607	No Hit
AGTGCAGTGCTGCTGTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA	3211	0.10719823995913715	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3177	0.10606316049522849	No Hit
CCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCTAG	3050	0.10182330485062854	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.07571647718072969	0.0	0.0	0.0	0.0
2	0.12492551041018099	0.0	0.0	0.0	0.0
3	0.15810989238445142	0.0	0.0	0.0	0.0
4	0.1895582704727439	0.0	0.0	0.0	0.0
5	0.21483048088976875	0.0	0.0	0.0	0.0
6	0.26941444922772867	0.0	0.0	0.0	0.0
7	0.2975243583045251	0.0	0.0	0.0	0.0
8	0.33257828292523334	0.0	0.0	0.0	0.0
9	0.36222388774731795	0.0	0.0	0.0	0.0
10	0.3916024150484829	0.0	0.0	0.0	0.0
11	0.4210477117298778	0.0	0.0	0.0	0.0
12	0.45857210341909305	0.0	0.0	0.0	0.0
13	0.4920235629142831	0.0	0.0	0.0	0.0
14	0.5222367074683221	0.0	0.0	0.0	0.0
15	0.5524498520223611	0.0	0.0	0.0	0.0
16	0.5828966894072047	0.0	0.0	0.0	0.0
17	0.6164149182826247	0.0	0.0	0.0	0.0
18	0.6500333012283896	0.0	0.0	0.0	0.0
19	0.6792115203888649	0.0	0.0	0.0	0.0
20	0.7106598984771574	0.0	0.0	0.0	0.0
21	0.7576989268491362	0.0	0.0	0.0	0.0
22	0.7949562410174318	0.0	0.0	0.0	0.0
23	0.837588490294236	0.0	0.0	0.0	0.0
24	0.8735438015480481	0.0	0.0	0.0	0.0
25	0.9070286457333532	0.0	0.0	0.0	0.0
26	0.9455211934359022	0.0	0.0	0.0	0.0
27	0.9787723447904025	0.0	0.0	0.0	0.0
28	1.011990111454788	0.0	0.0	0.0	0.0
29	1.0521852783532	0.0	0.0	0.0	0.0
30	1.0913121351679334	0.0	0.0	0.0	0.0
31	1.1312736092355407	0.0	0.0	0.0	0.0
32	1.1702335425996993	0.0	0.0	0.0	0.0
33	1.208959783133053	0.0	0.0	0.0	0.0
34	1.2431457058107722	0.0	0.0	0.0	0.0
35	1.27449392982872	0.0	0.0	0.0	0.0
36	1.298697830162066	0.0	0.0	0.0	0.0
37	1.3212324959896642	0.0	0.0	0.0	0.0
38	1.3331174456705899	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACGCGG	10	0.0024417376	207.07797	44
CGCGTAC	85	0.0	133.99162	44
TACGACT	15	2.7765802E-4	118.18435	43
CTATCAT	2250	0.0	113.66278	44
CGAATGG	145	0.0	105.958374	43
GATCTAT	55	4.878839E-7	94.12634	44
AATCGCA	305	0.0	81.47329	44
AAATCCG	20	4.124945E-5	79.86795	42
GCGTATA	30	4.1078238E-8	79.86795	42
GTATCCG	25	1.2976761E-6	79.86794	42
CGCAAAT	45	4.512367E-8	78.78956	43
CCTTAGC	8310	0.0	77.29626	43
GCGAAGT	95	1.877197E-9	76.291885	44
CTACGTC	95	1.877197E-9	76.291885	44
CAACGAC	55	5.52013E-5	75.30108	44
CTTGCGA	55	2.1045707E-9	75.20822	43
CGTACCA	235	0.0	72.92226	43
GCGCGTA	90	0.0	72.22376	43
TTACGTT	345	0.0	72.027115	44
CCCTATC	2205	0.0	69.36377	42
>>END_MODULE