##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR841033.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1293918 Sequences flagged as poor quality 0 Sequence length 24-50 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.420675807895094 38.0 35.0 39.0 32.0 39.0 2 37.35794076595271 39.0 37.0 39.0 35.0 39.0 3 37.508406251400785 39.0 37.0 39.0 34.0 39.0 4 38.07745235787739 39.0 37.0 40.0 35.0 41.0 5 38.51726539085166 40.0 38.0 41.0 35.0 41.0 6 38.41694450498409 40.0 38.0 41.0 35.0 41.0 7 38.393440697169375 40.0 38.0 41.0 34.0 41.0 8 38.34731489939857 40.0 38.0 41.0 34.0 41.0 9 38.43212552882022 40.0 38.0 41.0 35.0 41.0 10 38.364627433886845 40.0 38.0 41.0 34.0 41.0 11 38.32061150706613 40.0 38.0 41.0 34.0 41.0 12 38.281629902358574 40.0 37.0 41.0 34.0 41.0 13 38.107203856813186 39.0 37.0 40.0 34.0 41.0 14 38.09756259670242 39.0 37.0 40.0 34.0 41.0 15 37.93603304073365 39.0 37.0 40.0 34.0 41.0 16 37.94236883635594 39.0 37.0 40.0 34.0 41.0 17 37.982098556477304 39.0 37.0 40.0 34.0 41.0 18 38.01355650048921 39.0 37.0 40.0 34.0 41.0 19 38.087900469736105 39.0 37.0 40.0 34.0 41.0 20 38.02466539610702 39.0 37.0 40.0 34.0 41.0 21 37.93621465966159 39.0 36.0 40.0 33.0 41.0 22 37.8253652858991 39.0 36.0 40.0 33.0 41.0 23 37.79952052603024 39.0 36.0 40.0 33.0 41.0 24 37.59099958420858 39.0 36.0 40.0 33.0 41.0 25 37.4860290089327 39.0 35.0 40.0 33.0 41.0 26 37.305042359826274 38.0 35.0 40.0 33.0 41.0 27 37.192093692730005 38.0 35.0 40.0 33.0 41.0 28 37.02147075716464 38.0 35.0 40.0 32.0 41.0 29 36.936069857556774 38.0 35.0 40.0 32.0 41.0 30 36.841639007885966 38.0 35.0 40.0 32.0 41.0 31 36.71581282563021 38.0 35.0 40.0 32.0 41.0 32 36.47270846133573 38.0 35.0 40.0 31.0 41.0 33 36.33574878830063 37.0 34.0 40.0 31.0 41.0 34 36.290615580735135 37.0 34.0 40.0 31.0 41.0 35 36.124638653727025 37.0 34.0 40.0 31.0 41.0 36 36.066519479386514 37.0 34.0 40.0 31.0 41.0 37 35.939723286792386 36.0 34.0 39.0 31.0 41.0 38 35.787608312193896 36.0 34.0 39.0 31.0 41.0 39 35.64652052700557 36.0 34.0 39.0 30.0 41.0 40 35.51432899168461 36.0 34.0 39.0 30.0 41.0 41 35.30706213411111 35.0 34.0 39.0 30.0 41.0 42 35.13357237489607 35.0 33.0 39.0 30.0 40.0 43 34.89450710255468 35.0 33.0 38.0 29.0 40.0 44 34.693501434585016 35.0 33.0 38.0 29.0 40.0 45 34.398659019931166 35.0 32.0 38.0 28.0 40.0 46 33.98484368112961 35.0 32.0 37.0 27.0 40.0 47 34.00900570396065 35.0 32.0 37.0 28.0 39.0 48 34.77676245163313 35.0 33.0 37.0 30.0 40.0 49 34.992006367726496 35.0 33.0 38.0 30.0 40.0 50 35.1885127604608 36.0 34.0 38.0 30.0 40.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 19 1.0 20 8.0 21 12.0 22 42.0 23 74.0 24 164.0 25 344.0 26 648.0 27 1357.0 28 2927.0 29 6026.0 30 12313.0 31 23334.0 32 41787.0 33 69205.0 34 102793.0 35 122123.0 36 182860.0 37 238923.0 38 219941.0 39 183167.0 40 85869.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 0.0 39.3242848464895 22.869764544584744 37.80595060892576 2 24.128808780772815 17.01143349114859 35.33307365690871 23.52668407116989 3 20.481205145921148 23.20038827808254 29.8188138661028 26.499592709893516 4 20.323776313491273 24.176184271337135 34.70583143599517 20.794207979176424 5 18.847021217727864 23.98042225241476 34.93552141634942 22.237035113507968 6 20.670088830976923 23.624758292256544 29.53641575432137 26.16873712244516 7 24.406106105641932 21.121353903415827 25.53948550062678 28.933054490315463 8 25.215740100995582 23.142347505792486 29.15864838420982 22.483264009002117 9 24.502248210473926 25.21844506375211 26.9572724082979 23.322034317476067 10 27.700905312392283 20.408943997996783 27.202264749389066 24.687885940221868 11 27.879819277574004 19.999876344559702 25.19263199058982 26.927672387276473 12 24.50588058903269 22.723541986431904 26.373618730089543 26.39695869444586 13 26.028156343755942 21.506540599945282 25.418921446335858 27.046381609962918 14 23.62282617600188 23.116766286580756 23.948658261188115 29.31174927622925 15 25.743826115719852 29.382464731149888 24.33399952701794 20.53970962611232 16 23.556593230792057 29.098520926364728 22.467575225014258 24.87731061782895 17 21.067254648285285 31.933090041254548 22.29638972485119 24.703265585608982 18 22.888081006678938 24.77413560982999 22.956400637443792 29.381382746047276 19 24.847787881457712 22.677093911669825 22.73598481511193 29.739133391760532 20 21.869314747920658 22.77740938761189 27.20388772704298 28.149388137424474 21 25.219758902805278 29.11397785640203 24.203929460754082 21.462333780038612 22 26.380265210005582 24.317460611878033 22.020097100434494 27.28217707768189 23 27.197936808978625 23.90599713428517 26.410869931479432 22.485196125256778 24 26.867081221530263 25.320847225249203 23.297689652667326 24.514381900553204 25 26.26586729559504 23.831075226620143 23.736114295039417 26.166943182745396 26 26.389460168313256 30.518077018719026 23.197811083151002 19.89465172981672 27 27.54032987517599 26.494309377924946 22.613045564303636 23.352315182595426 28 26.132261195064466 24.93957338931691 21.682676120496723 27.245489295121907 29 24.231480623696243 26.688857866641964 25.354351083098802 23.725310426562995 30 27.907468514015925 27.488084211338816 25.339222080712183 19.265225193933077 31 26.55442249849203 29.091939551698033 22.353484116735014 22.00015383307492 32 23.612331638602015 26.630373142792337 25.035922265930026 24.721372952675626 33 22.850127292121037 24.700670399692328 30.563668248299347 21.885534059887284 34 24.978216335351043 28.20897768063081 24.951844460500883 21.860961523517265 35 22.505045526300012 23.729126840867764 25.554175297902525 28.211652334929692 36 23.710713243164346 27.208899685605285 24.381612103357682 24.698774967872687 37 23.291266397663385 32.11941382046564 22.64228191318735 21.947037868683626 38 27.870766769228677 25.475690425214797 21.84570857385017 24.807834231706355 39 22.112279783842563 31.57938382649271 23.366358810740845 22.94197757892388 40 21.583137963448813 27.21909601686686 25.276339608433084 25.921426411251236 41 22.27188720354734 31.78264894853714 23.775120599761934 22.170343248153586 42 20.631031095011117 31.77859741016846 23.050624542907638 24.539746951912786 43 22.770365715475904 25.83186540972648 23.9166367741344 27.481132100663213 44 22.761323478661517 26.113909055231748 23.603225926880324 27.52154153922641 45 23.944927275835987 24.340787837865253 30.73251591701418 20.98176896928458 46 20.821711095375445 28.308733773514668 27.493363587054382 23.37619154405551 47 22.056271941448564 32.0581127710491 25.766261640636117 20.119353646866216 48 27.440041581070712 24.82868433862172 23.73272611769753 23.998547962610036 49 19.300714913196046 26.85559529991895 22.92648479171419 30.917204995170817 50 21.808600791975472 29.144389146546278 27.125416660246632 21.921593401231622 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 13312.0 1 10841.0 2 9614.5 3 10194.0 4 8529.5 5 5467.5 6 2627.5 7 2067.5 8 2366.0 9 2369.5 10 2141.0 11 1836.5 12 1517.5 13 1351.5 14 1487.5 15 1675.0 16 1715.5 17 1771.5 18 2015.0 19 2422.5 20 2889.5 21 3409.5 22 4037.0 23 5283.0 24 6381.5 25 6922.0 26 7957.0 27 9792.0 28 11615.5 29 13244.0 30 15614.5 31 19158.5 32 24402.0 33 28477.5 34 32743.0 35 38562.5 36 41302.5 37 40732.5 38 40096.0 39 43215.5 40 48835.5 41 55990.5 42 62802.0 43 66229.5 44 68907.5 45 74293.0 46 83039.0 47 93132.0 48 109658.5 49 125256.5 50 142874.5 51 156177.0 52 143255.5 53 125260.0 54 120141.5 55 116840.5 56 114178.5 57 113075.5 58 107628.5 59 93620.0 60 75392.0 61 63362.0 62 54802.0 63 43823.0 64 32730.5 65 25869.5 66 19356.0 67 13339.0 68 10324.0 69 7810.0 70 5514.0 71 3776.5 72 2532.0 73 1798.0 74 1403.5 75 1199.5 76 1005.5 77 779.0 78 468.0 79 235.5 80 204.0 81 193.0 82 172.0 83 120.0 84 51.5 85 15.5 86 6.5 87 5.0 88 3.5 89 2.0 90 1.5 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 >>END_MODULE >>Sequence Length Distribution warn #Length Count 24 7073.0 25 8039.0 26 7416.0 27 9115.0 28 10127.0 29 8420.0 30 8623.0 31 9542.0 32 8677.0 33 7264.0 34 8558.0 35 8932.0 36 7512.0 37 7121.0 38 7796.0 39 9084.0 40 11363.0 41 10263.0 42 15524.0 43 25242.0 44 63014.0 45 129980.0 46 139274.0 47 159914.0 48 193967.0 49 217375.0 50 194703.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 12.778972297157843 #Duplication Level Percentage of deduplicated Percentage of total 1 68.02821960052285 8.693307337000515 2 11.422913025207125 2.9194617820393067 3 4.452665040484492 1.7070144960262355 4 2.372402007603947 1.2126743813156993 5 1.488239245948568 0.9509084047759915 6 1.0470766533091167 0.8028338127382767 7 0.808691770011352 0.7233974808140227 8 0.6138014383937967 0.6275001261751971 9 0.5452619049209968 0.6271098099912837 >10 5.351964111345177 16.311669401160028 >50 2.319497159320591 21.173463252755877 >100 1.4756432009242502 30.62964722368424 >500 0.048453955851244034 4.221279509578917 >1k 0.022653797540841368 5.202658241146355 >5k 0.0012585443078245205 1.326839484665907 >10k+ 0.0012585443078245205 2.8702352561321347 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGC 21276 1.6443082173677157 No Hit CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTA 14417 1.1142128017385955 No Hit CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCT 8399 0.6491137769163116 No Hit ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATCAT 8101 0.6260829511607381 No Hit ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATC 4955 0.38294544167404737 No Hit TCAGTTCTTTGACCATTCGTTGGAGCTCCGGTTTTCAGCCTCTTTCCG 3848 0.2973913339176053 No Hit CTCTCGGCCTTAGCGCCATTTTTTTGGAAACCTCTGCGCCATGAGAGCC 3226 0.24932028150160984 No Hit ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTA 3200 0.24731088059676115 No Hit CCTTTCCGGCGGTGACGACCTACGCACACGAGAACATGCCTCTCGCAAA 2703 0.2089004094540767 No Hit CTCTTTTTCCGGCTGGAACCATGGAGGG 2371 0.18324190559216272 No Hit TCAGTTCTTTGACCATTCGTTGGAGCTCCGGTTTTCAGCCTCTTTC 2341 0.1809233660865681 No Hit CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACC 2276 0.17589986382444636 No Hit TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCTA 2248 0.1737358936192247 No Hit CTCTCGGCCTTAGCGCCATTTTTTTGGAAACCTCTGCGCCATGAGAG 2014 0.15565128547558657 No Hit CGGCACAGCTGGTTTGAGCAACTGAACTGGAAACAAGATGCAGGACCC 1983 0.1532554613198054 No Hit CCTTTCCGGCGGTGACGACCTACGCACACGAGAACATGCCTCTCGCA 1848 0.14282203354462958 No Hit CTTTTTCGCAACGGGTTTGCCGCCAGAACACAGGTGTCGTGAAAACTAC 1786 0.13803038523306732 No Hit CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTT 1773 0.13702568478064298 No Hit TCAGTTCTTTGACCATTCGTTGGAGCTCCGGTTTTCAGCCTCTT 1590 0.1228825937965157 No Hit TCCCTGGTGTGATTCCGTCCTGCGCGGTTGTTCTCTGGAGCAGCGTTCT 1589 0.1228053091463292 No Hit CTTTTCGATCCGCCATCTGCGGTGGAGCCGCCACCAAAATGCAGATTTT 1576 0.12180060869390485 No Hit CTCTTTCCCATCTTGCAAGATGGCGGG 1561 0.12064133894110755 No Hit CTCTTTTCCGTGGCGCCTCGGAGGCGTTCAGCTGCTTCAAGATGAAGCT 1489 0.11507684412768043 No Hit TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTC 1477 0.11414942832544256 No Hit TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1361 0.10518440890380998 No Hit CCTTTTGGCTCTCTGACCAGCACCATGGCGGTTGGCAAGAACAAGCGCC 1361 0.10518440890380998 No Hit CTCTTCCTGCTCTCCATCATGGCGCAGGATCAAGGTGAAAAGGAGAACC 1348 0.10417970845138563 No Hit CTCTCGGCCTTAGCGCCATTTTTTTGGAAACCTCTGCGCCATGAG 1330 0.10278858474802885 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.034623523283546564 0.0 0.0 0.0 0.0 2 0.07025174701951746 0.0 0.0 0.0 0.0 3 0.08988204816688539 0.0 0.0 0.0 0.0 4 0.10920321071350735 0.0 0.0 0.0 0.0 5 0.1235008709980076 0.0 0.0 0.0 0.0 6 0.15000950601197294 0.0 0.0 0.0 0.0 7 0.16763040625449216 0.0 0.0 0.0 0.0 8 0.1916659324624899 0.0 0.0 0.0 0.0 9 0.2140011963663849 0.0 0.0 0.0 0.0 10 0.23285865101188793 0.0 0.0 0.0 0.0 11 0.24978398940272878 0.0 0.0 0.0 0.0 12 0.2711918375043859 0.0 0.0 0.0 0.0 13 0.28858088379634567 0.0 0.0 0.0 0.0 14 0.3119981328028515 0.0 0.0 0.0 0.0 15 0.3352608125089843 0.0 0.0 0.0 0.0 16 0.3533454206526225 0.0 0.0 0.0 0.0 17 0.3860368276815069 0.0 0.0 0.0 0.0 18 0.41416844034938843 0.0 0.0 0.0 0.0 19 0.4354990038008591 0.0 0.0 0.0 0.0 20 0.46069379976165414 0.0 0.0 0.0 0.0 21 0.48395647946778697 0.0 0.0 0.0 0.0 22 0.5043596271170198 0.0 0.0 0.0 0.0 23 0.5267721756711012 0.0 0.0 0.0 0.0 24 0.550807701879099 0.0 0.0 0.0 0.0 25 0.5739930969350453 0.0 0.0 0.0 0.0 26 0.6001153086980783 0.0 0.0 0.0 0.0 27 0.6223732879517867 0.0 0.0 0.0 0.0 28 0.649500200167244 0.0 0.0 0.0 0.0 29 0.6770135356336336 0.0 0.0 0.0 0.0 30 0.706999979905991 0.0 0.0 0.0 0.0 31 0.7324266298173454 0.0 0.0 0.0 0.0 32 0.7586261262305648 0.0 0.0 0.0 0.0 33 0.783202644989868 0.0 0.0 0.0 0.0 34 0.8093248567529009 0.0 0.0 0.0 0.0 35 0.8330512443601527 0.0 0.0 0.0 0.0 36 0.8573959091688963 0.0 0.0 0.0 0.0 37 0.8742439629095506 0.0 0.0 0.0 0.0 38 0.8859139450877104 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGAATAC 30 1.0913936E-11 261.0341 44 CGACGGG 10 0.0012202328 261.03406 44 CACGAAT 10 0.0012202328 261.03406 44 CTATCAT 1270 0.0 155.18167 44 CACGACA 60 7.2759576E-12 152.26988 44 GTAATAT 30 1.8680458E-4 130.51704 44 CATTGCG 20 0.009706003 130.51703 44 ATGACGT 20 0.009706003 130.51703 44 ACCCGGT 30 1.9190338E-9 124.09765 43 ATCCACC 65 2.3519533E-9 120.47726 44 CGTGCGC 55 1.2244709E-7 118.65185 44 AGTCATA 45 6.4476862E-6 116.015144 44 CGAGTAC 35 3.4502154E-4 111.87174 44 AACTACC 140 0.0 111.87174 44 CTCTAGA 120 0.0 108.7642 44 GAGTTAC 85 1.2369128E-10 107.48461 44 TCGTGCG 35 5.6079443E-9 106.36941 43 CCCAACG 30 2.7838723E-7 103.4147 43 CGACTAT 20 7.176163E-4 93.073235 43 CGAAATG 155 0.0 92.62499 44 >>END_MODULE