FastQCFastQC Report
Tue 24 May 2016
ERR841032.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR841032.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8016326
Sequences flagged as poor quality0
Sequence length24-50
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAAC1278201.5944960322222423No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATCAT1090361.3601742244514508No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTA846211.0556082674282457No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATC644230.8036474564532431No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCT467920.5837087962740038No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTA390090.4866194313953799No Hit
CGGCAAGTTTGAATTTCGTGGAGGCTCGGG288030.3593042498521143No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAGAA242780.3028569446901236No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACACA235890.29426198485440835No Hit
CAAAGCACCCAACTTACACTTAGGAGATTTCAACTTAACTTGACCGCTCT218200.27219451903527875No Hit
ATAGGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACATGC209740.26164105601493753No Hit
AACACAAAGCACCCAACTTACACTTAGGAGATTTCAACTTAACTTGACCG192110.23964843745127135No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACA149740.18679380055152447No Hit
ACAACCTTAACCAAACCATTTACCCAAATAAAGTATAGGCGATAGAAAT144500.18025714024105308No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCTA144270.17997022576177665No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAG141100.176015795764793No Hit
CAAAGCACCCAACTTACACTTAGGAGATTTCAACTTAACTTGACCGCT139800.17439410522975238No Hit
CAGAGTGTTGCTTAACACAAAGCACCCAACTTACACTTAGGAGATTTCAA136390.1701402862109151No Hit
ATAGGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACAT136370.1701153371257606No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACC134130.16732103958845987No Hit
AACACAAAGCACCCAACTTACACTTAGGAGATTTCAACTTAACTTGAC131900.16453921659373633No Hit
CTGATGTTCGCCGACCGTTGACTATTCTCTACAAACCACAAAGACATTGG130890.16327928779343556No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA128890.16078437927798844No Hit
AGGTTTTCTGTTTCCTGTGATACCTTGGGCTGGAATCTGAAGCAATGAA114280.14255907257264738No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCT112030.13975230049276938No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCC99520.12414664772864775No Hit
ACAACCTTAACCAAACCATTTACCCAAATAAAGTATAGGCGATAGAA94660.11808402003611129No Hit
AGCTTTAATTTATTAATGCAAACAGTACCTAACAAACCCACAGGTCCT94320.11765988558848531No Hit
ATGACCCCAATACGCAAAATTAACCCCCTAATAAAATTAATTAACCACTC94030.11729812385374548No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTC93920.11716090388539588No Hit
AGGTTTTCTGTTTCCTGTGATACCTTGGGCTGGAATCTGAAGCAATGAAG89130.11118559799090007No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAGAAT86810.10829150411298143No Hit
CAGAGTGTTGCTTAACACAAAGCACCCAACTTACACTTAGGAGATTTC85990.10726859162164812No Hit
CAAAGCACCCAACTTACACTTAGGAGATTTCAACTTAACTTGACCG83920.10468636130816038No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAA82950.10347633067816853No Hit
CTGATGTTCGCCGACCGTTGACTATTCTCTACAAACCACAAAGACATT80310.10018305143777836No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATCAT176050.0160.2734444
CGTGCGC6850.0149.0705444
CGTCCGC403.4606273E-8146.7145444
ACATTGG32050.0112.4277444
TCGTGCG17500.096.6147943
ACCACTC26650.091.166744
CACGACT656.2567597E-790.2858744
AACGTAT408.9591823E-488.0287244
ACTTCTA39300.087.80472644
AACGACA7950.084.9193243
CCAGAAT23900.083.9771944
CCTTAAC202450.082.0098543
GAACACA67150.080.75307544
CCTATCA180150.080.4879243
GTTACGA450.001430211778.2477544
AATCGCA15200.074.90163444
TCCAGAA59250.072.1971243
CCCTATC216850.070.2861442
AATGAAG37800.066.1379844
TGAACAC42700.065.48067543
Produced by FastQC (version 0.11.5)