FastQCFastQC Report
Tue 24 May 2016
ERR841031.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR841031.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4313833
Sequences flagged as poor quality0
Sequence length24-50
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGC346040.8021636442579024No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAA239870.5560484144842881No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAA230030.5332380738892767No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTA226170.524290115078632No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAA216560.5020129430137884No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAA183500.4253757621122561No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA180740.41897773975024066No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATCAT175820.40757256945273496No Hit
AAAAAAAAAAAAAAAAAAAAAAAA142290.32984587025042467No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCT133150.3086582164863591No Hit
CGGCAAGTTTGAATTTCGTGGAGGCTCGGG128760.2984816519322839No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACACA123710.28677512550903106No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAA121720.28216205866105615No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATC104090.24129353176166068No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCTA86390.2002627361791706No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAGAA83900.19449060730909148No Hit
ATAGGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACATGC83790.19423561366422856No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACA77380.1793764385408522No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAA71610.16600086280576926No Hit
CAGAGTGTTGCTTAACACAAAGCACCCAACTTACACTTAGGAGATTTCAA71550.16586177536311675No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA69490.1610864398320473No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAA68340.15842059718120752No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTA65060.1508171503162037No Hit
TAGCTGTTAGTTGGTAGAGGGAAATTCAGGCTACCGTCGCGAAACCTG62880.14576363989982924No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAA62010.14374687198136785No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA61760.14316734097031572No Hit
CAAAGCACCCAACTTACACTTAGGAGATTTCAACTTAACTTGACCGCTCT59170.13716339969581576No Hit
AGGTTTTCTGTTTCCTGTGATACCTTGGGCTGGAATCTGAAGCAATGAA57480.1332457700611034No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAA57400.13306032013756675No Hit
CACTTAGGAGATTTCAACTTAACTTGACCGCTCTGACCAAAAAAAAAAAA55130.1277981785572135No Hit
ATAGGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACAT54490.12631457916892008No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAA54410.12612912924538341No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTC53690.12446007993355329No Hit
AACACAAAGCACCCAACTTACACTTAGGAGATTTCAACTTAACTTGACCG52610.12195650596580813No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAA52440.1215624248782927No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAA50650.11741298283915952No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAA50240.11646255198103404No Hit
AGCTTTAATTTATTAATGCAAACAGTACCTAACAAACCCACAGGTCCT49350.11439942158168849No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAG48810.1131476345978159No Hit
CCCGAAGCGTTTACTTTGAAAAAATTAGAGTGTTCAAAGCAGGCCCGAGC48560.11256810358676379No Hit
CTGATGTTCGCCGACCGTTGACTATTCTCTACAAACCACAAAGACATTGG46510.1078159492961364No Hit
CAGAGTGTTGCTTAACACAAAGCACCCAACTTACACTTAGGAGATTTC44300.10269289515843567No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAA44290.10266971391799358No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAA44100.10222927034959396No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTACGA100.0015808913239.4498144
TGTAACG150.005318835159.6332144
CTATCAT32750.0141.1108744
CCCGAGC10000.0123.3166544
ACTTCTA20850.0109.6760544
ACATTGG11200.0107.9662144
CGACGAA302.2338281E-7107.327843
GCGTATC603.4496952E-799.7707644
AACGACA3600.094.8062243
CGTGCGC1800.093.1193744
ATAACGT552.6866208E-587.0726644
ACCGTGA708.636853E-785.5177944
CGACACC850.083.3368843
GAACACA37800.081.71744
CCTTAGC63100.075.7247843
TGAACAC22100.074.5952543
CCTATCA34700.072.5622543
GCCCGAG9350.071.6283943
CCCTATC36350.071.0795342
TCGCAAA3400.070.0787443
Produced by FastQC (version 0.11.5)