##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR841031.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4313833
Sequences flagged as poor quality	0
Sequence length	24-50
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.84685545314341	39.0	37.0	39.0	33.0	39.0
2	37.55955457710115	39.0	37.0	39.0	35.0	39.0
3	37.74971794225692	39.0	37.0	39.0	35.0	39.0
4	38.33957480505156	39.0	38.0	40.0	35.0	41.0
5	38.997067109459266	40.0	38.0	41.0	35.0	41.0
6	38.924854531920914	40.0	38.0	41.0	35.0	41.0
7	39.04287880407053	40.0	38.0	41.0	35.0	41.0
8	38.92974855540305	40.0	38.0	41.0	35.0	41.0
9	39.05415555029599	40.0	38.0	41.0	35.0	41.0
10	39.05036935829458	40.0	38.0	41.0	35.0	41.0
11	38.97274280205098	40.0	38.0	41.0	35.0	41.0
12	38.932987901942425	40.0	38.0	41.0	35.0	41.0
13	38.868519713210965	40.0	38.0	41.0	35.0	41.0
14	38.79841709217765	40.0	38.0	41.0	35.0	41.0
15	38.67687854397702	40.0	38.0	41.0	35.0	41.0
16	38.615313110173716	40.0	38.0	41.0	35.0	41.0
17	38.55925229372579	40.0	37.0	41.0	35.0	41.0
18	38.51540381836757	40.0	37.0	41.0	35.0	41.0
19	38.480282616410975	40.0	37.0	41.0	35.0	41.0
20	38.4058562304104	40.0	37.0	41.0	35.0	41.0
21	38.28939599655342	40.0	37.0	41.0	35.0	41.0
22	38.18771797610153	40.0	36.0	41.0	34.0	41.0
23	37.86491062588654	39.0	35.0	41.0	34.0	41.0
24	37.648775230751866	39.0	35.0	41.0	33.0	41.0
25	37.531603234766955	39.0	35.0	41.0	33.0	41.0
26	37.40686088866875	39.0	35.0	41.0	33.0	41.0
27	37.36555903653312	39.0	35.0	41.0	33.0	41.0
28	37.293107419762926	39.0	35.0	41.0	33.0	41.0
29	37.31886994243905	39.0	35.0	41.0	33.0	41.0
30	37.37007609420689	39.0	35.0	41.0	33.0	41.0
31	37.286801829038836	38.0	35.0	41.0	33.0	41.0
32	37.27684421991023	38.0	35.0	40.0	33.0	41.0
33	37.11102944400711	38.0	35.0	40.0	33.0	41.0
34	37.040968530641216	38.0	35.0	40.0	33.0	41.0
35	36.922694177424475	38.0	35.0	40.0	32.0	41.0
36	36.89317604897715	38.0	35.0	40.0	32.0	41.0
37	36.800459949880285	38.0	35.0	40.0	32.0	41.0
38	36.62864014370661	38.0	35.0	40.0	31.0	41.0
39	36.58138855863713	38.0	35.0	40.0	31.0	41.0
40	36.36623217360477	37.0	34.0	40.0	31.0	41.0
41	36.271565333952765	37.0	34.0	40.0	31.0	41.0
42	36.11615104274691	37.0	34.0	40.0	31.0	41.0
43	35.99823076711462	37.0	34.0	40.0	30.0	41.0
44	35.74403025623217	36.0	34.0	40.0	29.0	41.0
45	35.506663848892494	36.0	33.0	40.0	29.0	41.0
46	35.182907872696816	36.0	33.0	40.0	28.0	41.0
47	35.25379277570538	36.0	33.0	39.0	28.0	41.0
48	36.08026723981666	37.0	34.0	40.0	31.0	41.0
49	36.78430591314546	38.0	35.0	40.0	32.0	41.0
50	36.66171322106358	38.0	35.0	40.0	32.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	12.0
20	41.0
21	136.0
22	276.0
23	580.0
24	1102.0
25	1811.0
26	2961.0
27	5394.0
28	10025.0
29	18169.0
30	33357.0
31	60909.0
32	110176.0
33	201651.0
34	291436.0
35	273403.0
36	484749.0
37	736920.0
38	689249.0
39	574022.0
40	817453.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	51.61043554537229	18.21463649612769	30.174927958500014
2	29.534708459970517	28.868989596954727	25.69654411749365	15.899757825581101
3	23.21251193544117	27.294380658685675	25.8609918371898	23.63211556868335
4	24.396308341097118	31.96535424528488	23.923040136231513	19.715297277386494
5	18.009065255887283	30.509943245369026	24.170059434382367	27.31093206436132
6	18.635746909998602	36.27407458749562	25.646519000619634	19.443659501886142
7	30.77685204781919	28.68641414723287	19.575537578761164	20.961196226186782
8	18.588294910813655	28.192306007209826	29.117724306898296	24.10167477507822
9	25.624380915997442	32.21707933524547	23.98233774928237	18.176201999474713
10	24.505700614743315	26.254052950125796	23.324709139180865	25.91553729595003
11	20.404174199603926	27.364341642339884	30.976396165544656	21.25508799251153
12	21.202767932833748	34.47736618455096	22.7716511047136	21.548214777901695
13	18.841480418922103	28.11967918090478	28.976689640048654	24.062150760124464
14	23.676878543977015	29.506427346631174	24.232741508537767	22.583952600854044
15	17.20771295504485	33.23459670321034	24.227595273159626	25.33009506858518
16	20.267381699755184	33.252793976957385	27.468610861848386	19.01121346143905
17	22.148863898996556	33.754157845238794	23.82827522530427	20.26870303046038
18	26.542520306187097	29.62875475244406	20.43470389326615	23.394021048102697
19	26.80539557280034	27.63815845444179	23.64968694893845	21.90675902381942
20	27.935040600783573	28.18164263660647	23.70977272416433	20.173544038445623
21	27.619428012164587	33.865705974246104	19.772972203606397	18.74189380998291
22	28.19455458753271	32.78318840808163	18.05663316127444	20.96562384311122
23	27.25680386792905	31.503050767148384	21.68584180240635	19.55430356251621
24	23.679312574223434	35.332545325699904	20.78717465418805	20.200967445888608
25	22.04170895433962	34.26245224634773	22.101048297166933	21.594790502145724
26	22.400813353019384	38.622181881092146	22.309927314868577	16.66707745101989
27	22.643437329307073	37.16476900388264	19.632873682487904	20.558919984322387
28	22.50377824031587	35.079193431492264	20.404305300870554	22.012723027321314
29	22.14746089822491	37.07088095116331	21.453680887013608	19.32797726359817
30	24.4752905424332	34.84997793348609	22.60175282683127	18.072978697249447
31	20.97230278979109	36.42917594438133	22.13941326785302	20.45910799797456
32	20.645851862576787	35.59178956295116	23.527390530953316	20.234968043518727
33	20.280731830055394	33.804282233159114	26.034773829683715	19.880212107101766
34	20.01866603570208	35.62244656518653	23.675210978783813	20.683676420327576
35	19.604248205840292	34.49380772285035	22.540571986297582	23.361372085011777
36	19.872037586125984	35.156733292092404	23.430880564858466	21.540348556923142
37	18.38223209960353	38.600155106872286	21.412052577362775	21.605560216161408
38	20.611294171116917	35.598984963829864	22.193987696203354	21.595733168849858
39	21.00460268418575	36.57556807013593	23.246676664639267	19.17315258103906
40	18.959133816168695	35.029739466681136	24.245825690613138	21.76530102653703
41	20.427061109157577	36.58666219011174	22.34246147127889	20.64381522945179
42	19.970060091456403	38.28924886384961	21.211818503766473	20.528872540927516
43	20.41552615048853	33.07620138191588	22.237257167485087	24.2710153001105
44	19.8668951226679	34.15729354198877	21.136064687843174	24.83974664750016
45	18.39376897867531	33.31098439439363	26.413359989975703	21.881886636955358
46	19.259102530194834	33.849352023274264	24.758494225513534	22.133051221017368
47	20.060644038001353	33.98480114054529	24.39076292891358	21.563791892539776
48	20.64101302535153	33.486754538156774	22.835901286997952	23.036331149493748
49	18.682697049834765	34.23255705581509	21.33229260109277	25.75245329325738
50	18.46890435887807	35.507524769635175	23.649441058378347	22.37412981310841
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	251140.0
1	235770.5
2	162334.0
3	101259.5
4	93115.0
5	66912.0
6	31579.5
7	17357.5
8	16171.5
9	13213.5
10	10112.5
11	8371.0
12	7787.0
13	7723.0
14	8526.0
15	9438.5
16	9768.0
17	10285.0
18	11563.5
19	13307.0
20	15174.0
21	19085.0
22	24830.0
23	34113.0
24	46201.0
25	56573.0
26	69081.0
27	82159.0
28	90879.5
29	97185.0
30	102631.5
31	112416.0
32	128273.5
33	139136.0
34	161919.0
35	194250.5
36	201740.0
37	196712.0
38	194912.5
39	200855.0
40	214991.5
41	231879.5
42	250002.0
43	258312.0
44	260980.5
45	270885.5
46	283150.5
47	279931.0
48	292017.0
49	329861.0
50	370792.5
51	389009.0
52	362163.0
53	327478.0
54	297008.5
55	273208.5
56	254070.5
57	226974.0
58	195471.5
59	168034.5
60	151407.5
61	134748.0
62	116773.5
63	100348.5
64	84949.0
65	72805.0
66	59142.5
67	45500.5
68	35768.0
69	27257.0
70	19610.5
71	14168.0
72	10444.5
73	7150.0
74	4540.5
75	3077.0
76	2209.5
77	1489.5
78	1047.5
79	753.0
80	498.0
81	369.0
82	275.5
83	320.5
84	371.5
85	321.5
86	287.0
87	160.5
88	35.0
89	26.0
90	27.0
91	22.0
92	15.0
93	15.0
94	7.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
24	43479.0
25	50784.0
26	58223.0
27	67510.0
28	65759.0
29	58262.0
30	63502.0
31	38554.0
32	36488.0
33	27572.0
34	27141.0
35	26239.0
36	26436.0
37	27464.0
38	28343.0
39	37716.0
40	44270.0
41	42326.0
42	54117.0
43	83011.0
44	198431.0
45	372456.0
46	457940.0
47	446960.0
48	643295.0
49	595175.0
50	692380.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	11.801470021141192
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.33761511552596	8.418887261655884
2	12.337235811813754	2.9119503715373907
3	4.231458669455135	1.4981229789981831
4	2.0486641174506617	0.9670899266192665
5	1.2162580329994792	0.7176816357207754
6	0.8528542481561303	0.603896030521049
7	0.598086248744851	0.49408078542334066
8	0.4924774207759294	0.4649566013900853
9	0.38228825013455475	0.4060406991057726
>10	3.5419175015026476	9.268936756288545
>50	1.3108056092008733	11.441067859089395
>100	1.5104941625988246	30.457508031424712
>500	0.07573745376918309	6.182844637008102
>1k	0.053611905477062176	12.728537298427018
>5k	0.006524200137420265	4.89377840617618
>10k+	0.003971252257560161	8.544620720614276
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGC	34604	0.8021636442579024	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAA	23987	0.5560484144842881	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAA	23003	0.5332380738892767	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTA	22617	0.524290115078632	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAA	21656	0.5020129430137884	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAA	18350	0.4253757621122561	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA	18074	0.41897773975024066	No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATCAT	17582	0.40757256945273496	No Hit
AAAAAAAAAAAAAAAAAAAAAAAA	14229	0.32984587025042467	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCT	13315	0.3086582164863591	No Hit
CGGCAAGTTTGAATTTCGTGGAGGCTCGGG	12876	0.2984816519322839	No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACACA	12371	0.28677512550903106	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	12172	0.28216205866105615	No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATC	10409	0.24129353176166068	No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCTA	8639	0.2002627361791706	No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAGAA	8390	0.19449060730909148	No Hit
ATAGGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACATGC	8379	0.19423561366422856	No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACA	7738	0.1793764385408522	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAA	7161	0.16600086280576926	No Hit
CAGAGTGTTGCTTAACACAAAGCACCCAACTTACACTTAGGAGATTTCAA	7155	0.16586177536311675	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	6949	0.1610864398320473	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAA	6834	0.15842059718120752	No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTA	6506	0.1508171503162037	No Hit
TAGCTGTTAGTTGGTAGAGGGAAATTCAGGCTACCGTCGCGAAACCTG	6288	0.14576363989982924	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAA	6201	0.14374687198136785	No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	6176	0.14316734097031572	No Hit
CAAAGCACCCAACTTACACTTAGGAGATTTCAACTTAACTTGACCGCTCT	5917	0.13716339969581576	No Hit
AGGTTTTCTGTTTCCTGTGATACCTTGGGCTGGAATCTGAAGCAATGAA	5748	0.1332457700611034	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAA	5740	0.13306032013756675	No Hit
CACTTAGGAGATTTCAACTTAACTTGACCGCTCTGACCAAAAAAAAAAAA	5513	0.1277981785572135	No Hit
ATAGGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACAT	5449	0.12631457916892008	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	5441	0.12612912924538341	No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTC	5369	0.12446007993355329	No Hit
AACACAAAGCACCCAACTTACACTTAGGAGATTTCAACTTAACTTGACCG	5261	0.12195650596580813	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAA	5244	0.1215624248782927	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAA	5065	0.11741298283915952	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAA	5024	0.11646255198103404	No Hit
AGCTTTAATTTATTAATGCAAACAGTACCTAACAAACCCACAGGTCCT	4935	0.11439942158168849	No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAG	4881	0.1131476345978159	No Hit
CCCGAAGCGTTTACTTTGAAAAAATTAGAGTGTTCAAAGCAGGCCCGAGC	4856	0.11256810358676379	No Hit
CTGATGTTCGCCGACCGTTGACTATTCTCTACAAACCACAAAGACATTGG	4651	0.1078159492961364	No Hit
CAGAGTGTTGCTTAACACAAAGCACCCAACTTACACTTAGGAGATTTC	4430	0.10269289515843567	No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAA	4429	0.10266971391799358	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAA	4410	0.10222927034959396	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.16834216809041982	0.0	0.0	0.0	0.0
2	0.3341807622130945	0.0	0.0	0.0	0.0
3	0.45015650814484476	0.0	0.0	0.0	0.0
4	0.5362516351467477	0.0	0.0	0.0	0.0
5	0.5920256996504037	0.0	0.0	0.0	0.0
6	0.7145849178677061	0.0	0.0	0.0	0.0
7	0.7737202622354644	0.0	0.0	0.0	0.0
8	0.8377236670960605	0.0	0.0	0.0	0.0
9	0.9017270719566567	0.0	0.0	0.0	0.0
10	0.9649191334017798	0.0	0.0	0.0	0.0
11	1.0276475700380612	0.0	0.0	0.0	0.0
12	1.1059074377705396	0.0	0.0	0.0	0.0
13	1.1745470907195528	0.0	0.0	0.0	0.0
14	1.2293475431246412	0.0	0.0	0.0	0.0
15	1.283174383431162	0.0	0.0	0.0	0.0
16	1.3287487021403008	0.0	0.0	0.0	0.0
17	1.3732103213082194	0.0	0.0	0.0	0.0
18	1.4124097988957849	0.0	0.0	0.0	0.0
19	1.4473902907228908	0.0	0.0	0.0	0.0
20	1.481327626730103	0.0	0.0	0.0	0.0
21	1.5184407926778807	0.0	0.0	0.0	0.0
22	1.566402779152554	0.0	0.0	0.0	0.0
23	1.6201832569781909	0.0	0.0	0.0	0.0
24	1.6832130497402193	0.0	0.0	0.0	0.0
25	1.750879090590665	0.0	0.0	0.0	0.0
26	1.8289071459187225	0.0	0.0	0.0	0.0
27	1.8973613489441987	0.0	0.0	0.0	0.0
28	1.962732446990878	0.0	0.0	0.0	0.0
29	2.0317198185465224	0.0	0.0	0.0	0.0
30	2.093938267893078	0.0	0.0	0.0	0.0
31	2.1596570845463883	0.0	0.0	0.0	0.0
32	2.2248427326695306	0.0	0.0	0.0	0.0
33	2.2906310930441673	0.0	0.0	0.0	0.0
34	2.352849542390723	0.0	0.0	0.0	0.0
35	2.406096851686192	0.0	0.0	0.0	0.0
36	2.4506511958158788	0.0	0.0	0.0	0.0
37	2.48535351275768	0.0	0.0	0.0	0.0
38	2.5044316736415158	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTTACGA	10	0.0015808913	239.44981	44
TGTAACG	15	0.005318835	159.63321	44
CTATCAT	3275	0.0	141.11087	44
CCCGAGC	1000	0.0	123.31665	44
ACTTCTA	2085	0.0	109.67605	44
ACATTGG	1120	0.0	107.96621	44
CGACGAA	30	2.2338281E-7	107.3278	43
GCGTATC	60	3.4496952E-7	99.77076	44
AACGACA	360	0.0	94.80622	43
CGTGCGC	180	0.0	93.11937	44
ATAACGT	55	2.6866208E-5	87.07266	44
ACCGTGA	70	8.636853E-7	85.51779	44
CGACACC	85	0.0	83.33688	43
GAACACA	3780	0.0	81.717	44
CCTTAGC	6310	0.0	75.72478	43
TGAACAC	2210	0.0	74.59525	43
CCTATCA	3470	0.0	72.56225	43
GCCCGAG	935	0.0	71.62839	43
CCCTATC	3635	0.0	71.07953	42
TCGCAAA	340	0.0	70.07874	43
>>END_MODULE