FastQCFastQC Report
Tue 24 May 2016
ERR841030.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR841030.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1847080
Sequences flagged as poor quality0
Sequence length24-50
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATCAT117730.6373844121532365No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTA104080.5634839855339238No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCT93340.5053381553587284No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATC69500.3762695714316651No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAGAA58140.31476709184225915No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACACA50200.27178032353769194No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTA48070.2602486086146783No Hit
CGGCAAGTTTGAATTTCGTGGAGGCTCGGG42790.23166294908720791No Hit
ACAACCTTAGCCAAACCATTTACCCAAATAAAGTATAGGCGATAGAAAT34230.18531953136843018No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAG32500.17595339671264915No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACA31920.17281330532516187No Hit
AGCTTTAATTTATTAATGCAAACAGTACCTAACAAACCCACAGGTCCT30350.1643134027762739No Hit
AGGTTTTCTGTTTCCTGTGATACCTTGGGCTGGAATCTGAAGCAATGAA28620.1549472681204929No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCTA27810.15056196807934685No Hit
ATAGGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACATGC27420.14845052731879507No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCC26630.14417350629101067No Hit
ATGACCCACCAATCACATGCCTATCATATAGTAAAACCCAGCCCATGACC22410.12132663447170669No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACC21900.11856551963098513No Hit
ACAACCTTAGCCAAACCATTTACCCAAATAAAGTATAGGCGATAGAA21590.11688719492387986No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAA19000.10286506269354874No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCCGCT403.6379788E-12166.4562744
CGAGTTA251.6732574E-6152.1885744
GCACGTA201.311124E-4142.676844
AGCGTAC204.762258E-6123.3464943
CGACGAA204.762258E-6123.3464943
CGAATGG1450.093.573243
CGCCCGC551.4551915E-1189.70653543
CTATCAT30000.086.8743144
AACTCCG558.0788595E-786.4707944
CCGACGA204.2178202E-579.5068842
ATAAGTA1600.077.28326444
CCAGTAC505.2386044E-576.09428444
TAACGAG250.001783582774.0078943
AACGACA1000.074.0078943
AATCGGG250.001783582774.0078943
CATGACC5750.072.7858444
AATGCGA503.6379788E-1271.5561842
GAACGAC750.068.9059642
TCCGGGC3500.068.7216243
AGGGCGG851.3645877E-767.1420244
Produced by FastQC (version 0.11.5)