##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR841030.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1847080
Sequences flagged as poor quality	0
Sequence length	24-50
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	35.78818946661758	38.0	35.0	39.0	30.0	39.0
2	36.990736730406915	39.0	37.0	39.0	33.0	39.0
3	36.9843119951491	38.0	37.0	39.0	33.0	39.0
4	37.22969172964896	38.0	36.0	39.0	33.0	40.0
5	37.83699893886567	39.0	37.0	40.0	33.0	41.0
6	37.69237174350867	39.0	36.0	40.0	33.0	41.0
7	37.79152067046365	39.0	36.0	40.0	33.0	41.0
8	37.71404649500834	39.0	36.0	40.0	33.0	41.0
9	37.844227645797694	39.0	37.0	40.0	33.0	41.0
10	37.845054897459775	39.0	37.0	40.0	34.0	41.0
11	37.764136366589426	39.0	36.0	40.0	33.0	41.0
12	37.755186023344955	39.0	36.0	40.0	33.0	41.0
13	37.668943954782684	39.0	36.0	40.0	33.0	41.0
14	37.634213461246944	39.0	36.0	40.0	33.0	41.0
15	37.47407042467029	38.0	36.0	40.0	33.0	41.0
16	37.48758743530329	38.0	36.0	40.0	33.0	41.0
17	37.5238533252485	38.0	36.0	40.0	33.0	41.0
18	37.61084793295363	39.0	36.0	40.0	33.0	41.0
19	37.76602042142192	39.0	37.0	40.0	34.0	41.0
20	37.760632457717044	39.0	36.0	40.0	33.0	41.0
21	37.702372934577824	39.0	36.0	40.0	33.0	41.0
22	37.59375554929944	39.0	36.0	40.0	33.0	41.0
23	37.50927355609936	39.0	36.0	40.0	33.0	41.0
24	37.37020811226368	38.0	36.0	40.0	33.0	41.0
25	37.32929705860087	38.0	36.0	40.0	33.0	41.0
26	37.179472873116374	38.0	35.0	40.0	33.0	41.0
27	37.02957209394729	38.0	35.0	40.0	32.0	41.0
28	36.989112736343	38.0	35.0	40.0	32.0	41.0
29	36.869456432057504	38.0	35.0	40.0	32.0	41.0
30	36.9145512546727	38.0	35.0	40.0	32.0	41.0
31	36.88916673288006	38.0	35.0	40.0	32.0	41.0
32	36.76846957028638	38.0	35.0	40.0	31.0	41.0
33	36.67705956148243	38.0	35.0	40.0	31.0	41.0
34	36.60868466431915	38.0	35.0	40.0	31.0	41.0
35	36.49789524214485	38.0	34.0	40.0	31.0	41.0
36	36.564477624208834	38.0	34.0	40.0	31.0	41.0
37	36.39071300937899	38.0	34.0	40.0	31.0	41.0
38	36.33452643237385	38.0	34.0	40.0	31.0	41.0
39	36.27197755274858	37.0	34.0	40.0	31.0	41.0
40	36.13056814482054	37.0	34.0	40.0	31.0	41.0
41	35.922126814929115	37.0	34.0	40.0	30.0	41.0
42	35.83566043383098	37.0	34.0	40.0	30.0	41.0
43	35.66913574098686	36.0	34.0	39.0	30.0	41.0
44	35.42055561774598	36.0	33.0	39.0	29.0	41.0
45	35.18049113709323	36.0	33.0	39.0	29.0	40.0
46	34.84314842716021	36.0	32.0	39.0	28.0	40.0
47	34.80261212104595	36.0	33.0	39.0	28.0	40.0
48	35.51588697436276	36.0	34.0	39.0	30.0	40.0
49	36.1997724449006	37.0	34.0	39.0	31.0	40.0
50	35.62828777921733	36.0	34.0	39.0	30.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	1.0
20	3.0
21	20.0
22	66.0
23	132.0
24	298.0
25	572.0
26	943.0
27	2037.0
28	4358.0
29	9675.0
30	19581.0
31	38309.0
32	66025.0
33	103268.0
34	139979.0
35	178787.0
36	256499.0
37	342530.0
38	324292.0
39	256493.0
40	103212.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	55.150995084132795	15.336422894514584	29.512582021352625
2	24.321415423262664	25.4089156939602	31.142614288498606	19.127054594278537
3	24.82404660328735	23.865993893063646	25.27362106676484	26.036338436884165
4	19.64392446456028	26.38894904389631	30.387097472767827	23.58002901877558
5	17.6966888277714	26.489431968295907	29.60922104077788	26.204658163154814
6	20.71464148818676	29.674838122875023	27.679580743660264	21.930939645277952
7	24.377395673170625	27.679093488100136	24.6531823202027	23.29032851852654
8	22.188697836585312	27.07863221950322	26.793046321761913	23.939623622149554
9	21.86846265456829	28.67742599129437	27.576769820473395	21.877341533663948
10	25.6786928557507	24.50310760768348	26.869816142235315	22.94838339433051
11	22.918065270589256	25.198637850011913	26.651363232778223	25.231933646620615
12	21.766193126448233	27.060820321805227	26.87225242003595	24.30073413171059
13	22.070457153994415	25.616053446520997	28.33347770535115	23.980011694133445
14	18.892305693310522	27.961972410507396	26.579357688892742	26.566364207289343
15	20.707874049851657	30.289971197782446	27.712281005695477	21.28987374667042
16	22.256805335989778	32.53519067934253	22.718236351430367	22.489767633237324
17	21.82991532581155	35.0397925374104	21.1742317604002	21.95606037637785
18	21.459167984061327	29.325367607250364	23.92213656149165	25.293327847196657
19	23.547003919700284	24.61550122355285	27.010145743551984	24.82734911319488
20	22.625008120926	25.753730212010307	27.85071572427832	23.770545942785372
21	21.58666652229465	29.71500963683219	25.89525088247396	22.803072958399202
22	25.548812179223425	28.64992312190051	23.164670723520366	22.636593975355694
23	24.434891829265652	27.46421378608398	26.095946033739743	22.004948350910627
24	22.14121748922624	29.525521363449336	24.868874114819064	23.46438703250536
25	22.552433174899612	27.84773587916556	26.152947090543595	23.44688385539124
26	23.166656517161716	31.63348327169566	27.061103860158475	18.138756350984146
27	23.14169352346475	30.29518564301797	25.163127455741467	21.399993377775818
28	23.04346739181761	29.78342689373002	23.518667174050115	23.65443854040226
29	23.683060873456487	30.78353773650869	24.665594540547346	20.867806849487476
30	23.964634059188207	30.950262706680693	25.49202988953208	19.593073344599023
31	24.3890396031737	30.038990226903824	23.77138219503059	21.800587974891883
32	22.520216942798196	29.489418609302938	25.646456391411444	22.343908056487425
33	20.835229321441695	27.654968948034757	27.715390969067844	23.794410761455705
34	21.646741729339652	30.751814980654697	25.223840368647565	22.377602921358083
35	20.443127792077807	28.474454474877938	25.80928333242925	25.27313440061501
36	21.916297748916733	28.471623668666844	27.106941133644124	22.5051374487723
37	18.668941939161297	30.991541047736966	24.7036173757972	25.635899637304536
38	21.668889709297478	28.487358429896748	25.669890078481345	24.17386178232443
39	21.199336219145966	29.897646142957395	27.45239637713252	21.450621260764116
40	20.391045423437234	29.095668950553637	27.079373075745195	23.433912550263937
41	20.17266088688905	30.295552370791206	25.730000073244153	23.801786669075586
42	19.95478412529884	31.629311119952014	25.606398951573073	22.809505803176073
43	20.915149043983305	26.735785807439594	26.431628387610846	25.917436760966257
44	20.18410768894158	29.005197706190945	24.38495082150726	26.42574378336022
45	19.002842464650367	28.389350554535596	29.00635863905262	23.601448341761415
46	19.444838564621822	31.49275456972962	27.00038511171618	22.06202175393238
47	22.27121091643589	29.84489960059591	26.276493652491016	21.607395830477184
48	21.027551165442617	30.37474489159317	24.69286629552756	23.904837647436654
49	18.80098044511668	32.492943766205705	24.280871866897147	24.425203921780465
50	19.175655214398326	30.595143322458622	28.191519806024278	22.037681657118775
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	22307.0
1	18751.5
2	15125.5
3	14442.5
4	12540.5
5	8842.0
6	5218.5
7	4114.5
8	4102.5
9	3910.0
10	3569.5
11	3043.5
12	2681.0
13	2642.0
14	2969.0
15	3312.5
16	3768.5
17	4651.0
18	5322.5
19	5935.5
20	7002.0
21	8552.5
22	10011.5
23	11866.0
24	14111.5
25	16205.0
26	18858.0
27	22598.5
28	26627.5
29	31822.0
30	37620.0
31	43745.0
32	52951.5
33	63537.5
34	83644.5
35	106378.0
36	109441.5
37	101679.0
38	98889.0
39	103961.5
40	113243.0
41	120935.5
42	127297.0
43	134107.0
44	138743.0
45	140096.5
46	144816.5
47	143945.0
48	147897.0
49	168379.0
50	187091.0
51	189914.5
52	174999.0
53	149169.0
54	126417.5
55	114986.0
56	105040.0
57	93822.0
58	81758.5
59	68123.0
60	56120.0
61	45645.5
62	37762.5
63	31936.5
64	26425.5
65	22349.5
66	17640.0
67	12741.5
68	9133.0
69	6871.5
70	5266.5
71	4249.5
72	3568.0
73	2703.5
74	1897.5
75	1292.5
76	960.5
77	737.5
78	553.5
79	468.0
80	413.5
81	259.5
82	138.0
83	86.0
84	39.5
85	26.0
86	8.0
87	2.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
24	10981.0
25	13350.0
26	10669.0
27	11995.0
28	12360.0
29	10648.0
30	15097.0
31	12210.0
32	13648.0
33	10897.0
34	10776.0
35	11269.0
36	11954.0
37	11846.0
38	10757.0
39	13112.0
40	17155.0
41	14590.0
42	17361.0
43	34173.0
44	81976.0
45	158175.0
46	237951.0
47	172463.0
48	329283.0
49	208006.0
50	384378.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	12.843804184881638
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.19549129002262	8.758895364208517
2	11.363869030418408	2.9191061721866958
3	4.2658873884277835	1.6437066687516773
4	2.2596425018223414	1.1608962328496881
5	1.4320309714342787	0.9196362691893852
6	1.0141386723006407	0.7815239114007164
7	0.7577769313409465	0.681291696537453
8	0.6078291964794299	0.6245471341948596
9	0.506353149770872	0.5853150633649611
>10	5.4527288034133585	17.240361095385484
>50	2.627287257559862	24.095841182860607
>100	1.4511099292309928	28.84524937523375
>500	0.04098660225374749	3.6130423723207525
>1k	0.02210513379977393	5.214613132508838
>5k	0.001842094483314494	1.6039958590235441
>10k+	9.21047241657247E-4	1.3119784699830823
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATCAT	11773	0.6373844121532365	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTA	10408	0.5634839855339238	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCT	9334	0.5053381553587284	No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATC	6950	0.3762695714316651	No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAGAA	5814	0.31476709184225915	No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACACA	5020	0.27178032353769194	No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTA	4807	0.2602486086146783	No Hit
CGGCAAGTTTGAATTTCGTGGAGGCTCGGG	4279	0.23166294908720791	No Hit
ACAACCTTAGCCAAACCATTTACCCAAATAAAGTATAGGCGATAGAAAT	3423	0.18531953136843018	No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAG	3250	0.17595339671264915	No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACA	3192	0.17281330532516187	No Hit
AGCTTTAATTTATTAATGCAAACAGTACCTAACAAACCCACAGGTCCT	3035	0.1643134027762739	No Hit
AGGTTTTCTGTTTCCTGTGATACCTTGGGCTGGAATCTGAAGCAATGAA	2862	0.1549472681204929	No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCTA	2781	0.15056196807934685	No Hit
ATAGGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACATGC	2742	0.14845052731879507	No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCC	2663	0.14417350629101067	No Hit
ATGACCCACCAATCACATGCCTATCATATAGTAAAACCCAGCCCATGACC	2241	0.12132663447170669	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACC	2190	0.11856551963098513	No Hit
ACAACCTTAGCCAAACCATTTACCCAAATAAAGTATAGGCGATAGAA	2159	0.11688719492387986	No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAA	1900	0.10286506269354874	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.03053468176797973	0.0	0.0	0.0	0.0
2	0.06442601295017	0.0	0.0	0.0	0.0
3	0.08797669835632457	0.0	0.0	0.0	0.0
4	0.10681724668124824	0.0	0.0	0.0	0.0
5	0.12289668016545033	0.0	0.0	0.0	0.0
6	0.1503454100526236	0.0	0.0	0.0	0.0
7	0.17243432877839618	0.0	0.0	0.0	0.0
8	0.19809645494510253	0.0	0.0	0.0	0.0
9	0.21850704896376985	0.0	0.0	0.0	0.0
10	0.23583169110163069	0.0	0.0	0.0	0.0
11	0.2544556813998311	0.0	0.0	0.0	0.0
12	0.27405418281828614	0.0	0.0	0.0	0.0
13	0.2926781731164866	0.0	0.0	0.0	0.0
14	0.3130887671351539	0.0	0.0	0.0	0.0
15	0.33550252290101135	0.0	0.0	0.0	0.0
16	0.35526344283950884	0.0	0.0	0.0	0.0
17	0.38309114927344784	0.0	0.0	0.0	0.0
18	0.41254304090781124	0.0	0.0	0.0	0.0
19	0.4334950299932867	0.0	0.0	0.0	0.0
20	0.4574788314528878	0.0	0.0	0.0	0.0
21	0.4789722156051714	0.0	0.0	0.0	0.0
22	0.4983000194902224	0.0	0.0	0.0	0.0
23	0.5201182406825909	0.0	0.0	0.0	0.0
24	0.5409619507547048	0.0	0.0	0.0	0.0
25	0.5645126361608593	0.0	0.0	0.0	0.0
26	0.5903371808476081	0.0	0.0	0.0	0.0
27	0.6162700045477185	0.0	0.0	0.0	0.0
28	0.6428525023279988	0.0	0.0	0.0	0.0
29	0.6693808606015982	0.0	0.0	0.0	0.0
30	0.6956926608484744	0.0	0.0	0.0	0.0
31	0.7220586006020313	0.0	0.0	0.0	0.0
32	0.746746215648483	0.0	0.0	0.0	0.0
33	0.7740325270156139	0.0	0.0	0.0	0.0
34	0.8019143729562336	0.0	0.0	0.0	0.0
35	0.8244905472421336	0.0	0.0	0.0	0.0
36	0.8432769560603763	0.0	0.0	0.0	0.0
37	0.8600602031314291	0.0	0.0	0.0	0.0
38	0.8734868007882712	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCCCGCT	40	3.6379788E-12	166.45627	44
CGAGTTA	25	1.6732574E-6	152.18857	44
GCACGTA	20	1.311124E-4	142.6768	44
AGCGTAC	20	4.762258E-6	123.34649	43
CGACGAA	20	4.762258E-6	123.34649	43
CGAATGG	145	0.0	93.5732	43
CGCCCGC	55	1.4551915E-11	89.706535	43
CTATCAT	3000	0.0	86.87431	44
AACTCCG	55	8.0788595E-7	86.47079	44
CCGACGA	20	4.2178202E-5	79.50688	42
ATAAGTA	160	0.0	77.283264	44
CCAGTAC	50	5.2386044E-5	76.094284	44
TAACGAG	25	0.0017835827	74.00789	43
AACGACA	100	0.0	74.00789	43
AATCGGG	25	0.0017835827	74.00789	43
CATGACC	575	0.0	72.78584	44
AATGCGA	50	3.6379788E-12	71.55618	42
GAACGAC	75	0.0	68.90596	42
TCCGGGC	350	0.0	68.72162	43
AGGGCGG	85	1.3645877E-7	67.14202	44
>>END_MODULE