FastQCFastQC Report
Tue 24 May 2016
ERR841029.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR841029.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17006606
Sequences flagged as poor quality0
Sequence length24-50
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGC899350.5288239170120128No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTA546320.32123987584589186No Hit
CGGCAAGTTTGAATTTCGTGGAGGCTCGGG382560.22494788201714086No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCT352470.2072547573572293No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAGAA271560.1596791270403983No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATCAT244560.14380294339740687No Hit
CAAAGCACCCAACTTACACTTAGGAGATTTCAACTTAACTTGACCGCTCT182630.10738768217479724No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATCAT67500.077.4691944
CCTTAGC149500.074.95952643
CGTTGCG1552.910383E-1158.50274744
TCGTGCG38050.057.92399643
CTATACG59000.056.86665344
ACCTTAG142700.056.51097542
CCCTATC59300.053.8529842
AACCTTA203600.049.8007641
CCGCTCT74450.049.39620644
CCTATCA65550.045.50020243
CGCGTAA707.9710723E-443.180644
CAACCTT223700.039.9363940
GACAACC248150.039.39090738
CGTGCGC30250.038.63652444
ACAACCT252450.038.44388239
GACGCTA65250.038.28474431
CCCCTAT71750.038.17815841
CCGCTCG1854.4583066E-738.12341344
CCCATAC25900.037.73439844
GAACACA88500.037.34186644
Produced by FastQC (version 0.11.5)