##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR841029.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	17006606
Sequences flagged as poor quality	0
Sequence length	24-50
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	37.19523654514016	39.0	37.0	39.0	34.0	39.0
2	37.842740873752234	39.0	37.0	39.0	35.0	39.0
3	37.90676628834701	39.0	37.0	39.0	35.0	39.0
4	38.412323129024095	39.0	38.0	40.0	35.0	41.0
5	39.11180173163299	40.0	38.0	41.0	35.0	41.0
6	39.10136955016186	40.0	38.0	41.0	35.0	41.0
7	39.1816135447602	40.0	38.0	41.0	35.0	41.0
8	39.09845527085181	40.0	38.0	41.0	35.0	41.0
9	39.19699198064564	40.0	39.0	41.0	35.0	41.0
10	39.19467264661744	40.0	39.0	41.0	35.0	41.0
11	39.15932520574652	40.0	39.0	41.0	35.0	41.0
12	39.13559736728187	40.0	39.0	41.0	35.0	41.0
13	39.02997929157646	40.0	38.0	41.0	35.0	41.0
14	39.039475366219456	40.0	38.0	41.0	35.0	41.0
15	38.924049043060094	40.0	38.0	41.0	35.0	41.0
16	38.942814868528146	40.0	38.0	41.0	35.0	41.0
17	38.86748467036868	40.0	38.0	41.0	35.0	41.0
18	38.89593849589977	40.0	38.0	41.0	35.0	41.0
19	38.87528528619996	40.0	38.0	41.0	35.0	41.0
20	38.81333865205086	40.0	38.0	41.0	35.0	41.0
21	38.71352961313974	40.0	38.0	41.0	35.0	41.0
22	38.608388293349066	40.0	38.0	41.0	35.0	41.0
23	38.55827082723031	40.0	38.0	41.0	35.0	41.0
24	38.44156723569653	40.0	38.0	41.0	34.0	41.0
25	38.34871584016502	40.0	37.0	41.0	34.0	41.0
26	38.262074235320966	40.0	37.0	41.0	34.0	41.0
27	38.18828560633169	40.0	37.0	41.0	34.0	41.0
28	38.09670317148992	40.0	37.0	41.0	34.0	41.0
29	38.05641654040539	39.0	37.0	41.0	34.0	41.0
30	38.04189253299129	39.0	36.0	41.0	34.0	41.0
31	37.95210573503643	39.0	36.0	41.0	33.0	41.0
32	37.906899738015206	39.0	36.0	41.0	33.0	41.0
33	37.80758984961676	39.0	36.0	41.0	33.0	41.0
34	37.70317261533837	39.0	36.0	41.0	33.0	41.0
35	37.63127859952429	39.0	36.0	41.0	33.0	41.0
36	37.565267833661295	39.0	35.0	41.0	33.0	41.0
37	37.479728948619254	39.0	35.0	41.0	33.0	41.0
38	37.34964079724322	38.0	35.0	41.0	32.0	41.0
39	37.250154055348204	38.0	35.0	41.0	32.0	41.0
40	37.06668395293197	38.0	35.0	40.0	32.0	41.0
41	36.954646321754154	38.0	35.0	40.0	31.0	41.0
42	36.815108796732055	38.0	35.0	40.0	31.0	41.0
43	36.66768489674053	38.0	35.0	40.0	31.0	41.0
44	36.46044472253809	38.0	34.0	40.0	31.0	41.0
45	36.21266572419799	37.0	34.0	40.0	30.0	41.0
46	35.84596307068899	37.0	34.0	40.0	29.0	41.0
47	35.88424615963355	37.0	34.0	40.0	29.0	41.0
48	36.72405462254004	38.0	35.0	40.0	31.0	41.0
49	37.26913563046644	38.0	35.0	40.0	33.0	41.0
50	36.93731195376697	38.0	35.0	40.0	32.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	5.0
20	21.0
21	80.0
22	210.0
23	427.0
24	957.0
25	1944.0
26	4139.0
27	9300.0
28	20174.0
29	43439.0
30	88201.0
31	170464.0
32	313559.0
33	545628.0
34	879675.0
35	918532.0
36	1478541.0
37	2570337.0
38	2919436.0
39	2907785.0
40	4133752.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	54.55781124111419	22.43785150311591	23.004337255769904
2	33.01410640077156	23.161717276216077	29.14475704323367	14.679419279778694
3	26.116516134965433	19.666657768163738	29.069239329705177	25.147586767165652
4	26.197008386035403	24.726244613416693	28.926347796850237	20.15039920369767
5	22.98465666812061	25.288038071793984	24.975259613823006	26.752045646262403
6	21.32228499913504	32.773211774295234	27.168178059749255	18.73632516682047
7	32.412575442742664	27.491575920556986	22.22851520168104	17.867333435019308
8	22.122091850660855	24.888093485554965	31.714205644559534	21.275609019224646
9	27.466168146660188	26.092155013175468	27.523539970291544	18.9181368698728
10	23.99060694414864	23.903946501729976	26.600910258049137	25.504536296072246
11	22.82362512543655	24.055469974432288	33.246198565428045	19.874706334703117
12	24.923221011882088	24.516925952185876	26.022346845690436	24.537506190241604
13	20.796730399939882	24.98686098801842	32.81476621496376	21.401642397077936
14	24.291913389420557	27.23190623690582	27.138989402118213	21.337190971555405
15	19.490179286801844	29.135213692843827	31.72327270944009	19.651334310914244
16	26.067840931929627	32.20181616484794	23.33265673350697	18.397686169715463
17	22.31911528967038	31.862900804546186	26.498685275592322	19.319298630191113
18	26.952632406489574	27.131539355942035	24.523123543874657	21.39270469369373
19	26.09715895105702	26.50735249584779	25.78796733457575	21.607521218519437
20	26.52884414444599	25.556768940257683	25.977452526388863	21.936934388907464
21	24.81553344623848	28.321629841956707	25.87912014895859	20.983716562846226
22	22.983004369008135	29.911029866864673	25.606784798801126	21.49918096532606
23	24.52915061359098	28.41592849272806	27.081817500799392	19.973103392881566
24	24.274408426937157	29.191127259607235	25.597411970383742	20.937052343071862
25	22.19857451192939	29.883013513503137	26.52108621291053	21.397325761656944
26	22.662075136799345	31.601146585603203	26.14115894060159	19.595619336995863
27	23.24101726862835	29.6608632074775	25.26410619136764	21.834013332526514
28	22.52456591999324	29.336218961434806	25.8957274407601	22.243487677811853
29	22.56529990743544	30.133223609378394	26.989646725047074	20.311829758139098
30	23.468495844763535	30.62022437715799	26.68189801864313	19.229381759435345
31	22.01530247655609	31.305912200113813	25.644922752370935	21.033862570959165
32	21.341908364418245	31.269489758124674	26.912596658618938	20.476005218838147
33	22.346372239477134	29.29061277524074	27.38541460142136	20.97760038386077
34	20.644534627894455	31.9160888811039	26.557634678794855	20.881741812206798
35	22.110506452441207	29.96470075696298	26.54834529895334	21.376447491642473
36	22.081744633992646	30.700182507191066	25.924595252753257	21.293477606063032
37	21.444245455393578	32.80338554349843	25.714395932372142	20.037973068735855
38	23.007911007505268	30.982960449345477	25.135276694288976	20.873851848860276
39	22.193902905751845	31.736408216855576	26.563111361949925	19.50657751544265
40	21.34298451486223	30.63954432644827	26.61442162087548	21.403049537814024
41	21.231160057197588	31.915070691517595	26.56505434625253	20.288714905032286
42	22.18307544183464	32.3358662352481	25.084229603774798	20.396828719142455
43	21.03918295054089	31.43906560648419	26.057993179506276	21.463758263468634
44	20.88507551433302	30.650776048684957	25.601348593099942	22.86279984388208
45	21.6374364686226	30.522350770384616	26.867274701177646	20.972938059815142
46	21.50504655007396	32.12712551739568	26.48494114429901	19.882886788231346
47	21.830117831640905	32.373749028009385	26.1675425095364	19.628590630813306
48	22.424876606480503	31.712566746945374	25.841590707524592	20.02096593904953
49	20.833411888464283	31.993837869239737	24.979221502142398	22.19352874015358
50	20.825938682764942	32.553184244653686	26.729526333370714	19.891350739210658
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	107744.0
1	110066.0
2	104773.5
3	95725.5
4	89130.5
5	72305.5
6	53286.5
7	46060.0
8	46111.5
9	45982.0
10	45545.0
11	44821.5
12	44661.0
13	46457.0
14	51693.5
15	59321.0
16	67564.0
17	77895.0
18	91418.0
19	107140.0
20	120059.5
21	141734.0
22	172817.0
23	206518.5
24	234306.0
25	257631.0
26	293828.5
27	337611.0
28	380349.0
29	417894.5
30	459305.0
31	520191.5
32	608175.5
33	687781.0
34	772162.5
35	860554.0
36	910071.5
37	946086.5
38	986167.5
39	1031261.5
40	1081794.0
41	1155017.0
42	1219630.0
43	1240506.0
44	1248261.5
45	1242127.0
46	1233787.5
47	1232036.5
48	1257433.0
49	1297801.5
50	1365143.5
51	1402122.5
52	1315559.5
53	1178756.5
54	1049026.5
55	962475.5
56	891096.5
57	807901.5
58	715455.0
59	611029.5
60	533010.5
61	469106.5
62	404888.0
63	336504.5
64	261128.0
65	201526.5
66	148296.5
67	105590.5
68	77444.0
69	56443.0
70	43079.0
71	32519.0
72	24891.5
73	17692.0
74	11024.0
75	7027.0
76	5544.5
77	4398.0
78	3206.0
79	2314.5
80	1625.5
81	1100.0
82	641.5
83	333.5
84	179.0
85	122.5
86	86.5
87	67.5
88	46.5
89	39.5
90	30.0
91	19.0
92	16.5
93	16.0
94	8.0
95	0.0
96	0.0
97	0.0
98	1.5
99	3.0
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
24	71915.0
25	73499.0
26	70976.0
27	80278.0
28	71821.0
29	63970.0
30	110276.0
31	67118.0
32	70000.0
33	67549.0
34	69905.0
35	71030.0
36	72160.0
37	75324.0
38	73901.0
39	103999.0
40	112503.0
41	105098.0
42	140331.0
43	261807.0
44	687784.0
45	1613202.0
46	1973135.0
47	1934008.0
48	2706143.0
49	2852132.0
50	3406742.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	11.932718330541832
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.16947963481566	8.373126358829467
2	12.478574671174613	2.9780663343552063
3	4.36475876549608	1.5625031078828466
4	2.021102931212749	0.964690079807768
5	1.127155283444831	0.6725013256064606
6	0.7429474129462081	0.5319229327855111
7	0.5311341613802971	0.4436512040435745
8	0.42866717426744233	0.4092131718466144
9	0.3285186160885947	0.3528108100912148
>10	3.772698210093005	10.451368480556512
>50	1.8084192202222256	16.060833794228863
>100	2.148005161013058	41.965446977395736
>500	0.050799187445769146	4.099836741402127
>1k	0.02459121425026576	5.511230273402439
>5k	0.0017018141349644876	1.362881725837969
>10k+	0.0014465420147198142	4.259916681927619
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGC	89935	0.5288239170120128	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTA	54632	0.32123987584589186	No Hit
CGGCAAGTTTGAATTTCGTGGAGGCTCGGG	38256	0.22494788201714086	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCT	35247	0.2072547573572293	No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAGAA	27156	0.1596791270403983	No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATCAT	24456	0.14380294339740687	No Hit
CAAAGCACCCAACTTACACTTAGGAGATTTCAACTTAACTTGACCGCTCT	18263	0.10738768217479724	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.039572857747160135	0.0	0.0	0.0	0.0
2	0.07291872346545807	0.0	0.0	0.0	0.0
3	0.09093525186624539	0.0	0.0	0.0	0.0
4	0.11146256930983173	0.0	0.0	0.0	0.0
5	0.12915569396974327	0.0	0.0	0.0	0.0
6	0.17044553157755285	0.0	0.0	0.0	0.0
7	0.194794893231489	0.0	0.0	0.0	0.0
8	0.2194500184222531	0.0	0.0	0.0	0.0
9	0.24142971266577234	0.0	0.0	0.0	0.0
10	0.26252739670690317	0.0	0.0	0.0	0.0
11	0.28497161632368034	0.0	0.0	0.0	0.0
12	0.3069748308392633	0.0	0.0	0.0	0.0
13	0.32872520243016157	0.0	0.0	0.0	0.0
14	0.35299224313187477	0.0	0.0	0.0	0.0
15	0.3761655911826263	0.0	0.0	0.0	0.0
16	0.4002209494357663	0.0	0.0	0.0	0.0
17	0.42555816251637746	0.0	0.0	0.0	0.0
18	0.45023092791118935	0.0	0.0	0.0	0.0
19	0.4753446984071954	0.0	0.0	0.0	0.0
20	0.5030339386941757	0.0	0.0	0.0	0.0
21	0.5300234508872611	0.0	0.0	0.0	0.0
22	0.5593885105587794	0.0	0.0	0.0	0.0
23	0.5892122155355396	0.0	0.0	0.0	0.0
24	0.6177187852767331	0.0	0.0	0.0	0.0
25	0.6480422960348466	0.0	0.0	0.0	0.0
26	0.6811647191685396	0.0	0.0	0.0	0.0
27	0.7113353481582392	0.0	0.0	0.0	0.0
28	0.7436580820417666	0.0	0.0	0.0	0.0
29	0.776357140278313	0.0	0.0	0.0	0.0
30	0.8107261378313815	0.0	0.0	0.0	0.0
31	0.8435898379723737	0.0	0.0	0.0	0.0
32	0.8788467257958467	0.0	0.0	0.0	0.0
33	0.9120691100858102	0.0	0.0	0.0	0.0
34	0.9459147815854616	0.0	0.0	0.0	0.0
35	0.9757914071743651	0.0	0.0	0.0	0.0
36	1.0020753112055398	0.0	0.0	0.0	0.0
37	1.0230730340904	0.0	0.0	0.0	0.0
38	1.0380436872589394	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTATCAT	6750	0.0	77.46919	44
CCTTAGC	14950	0.0	74.959526	43
CGTTGCG	155	2.910383E-11	58.502747	44
TCGTGCG	3805	0.0	57.923996	43
CTATACG	5900	0.0	56.866653	44
ACCTTAG	14270	0.0	56.510975	42
CCCTATC	5930	0.0	53.85298	42
AACCTTA	20360	0.0	49.80076	41
CCGCTCT	7445	0.0	49.396206	44
CCTATCA	6555	0.0	45.500202	43
CGCGTAA	70	7.9710723E-4	43.1806	44
CAACCTT	22370	0.0	39.93639	40
GACAACC	24815	0.0	39.390907	38
CGTGCGC	3025	0.0	38.636524	44
ACAACCT	25245	0.0	38.443882	39
GACGCTA	6525	0.0	38.284744	31
CCCCTAT	7175	0.0	38.178158	41
CCGCTCG	185	4.4583066E-7	38.123413	44
CCCATAC	2590	0.0	37.734398	44
GAACACA	8850	0.0	37.341866	44
>>END_MODULE