FastQCFastQC Report
Tue 24 May 2016
ERR841028.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR841028.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences985949
Sequences flagged as poor quality0
Sequence length24-50
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGC251062.546379173770651No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTA157071.5930844293163235No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCT106461.0797718746101472No Hit
CCCGAAGCGTTTACTTTGAAAAAATTAGAGTGTTCAAAGCAGGCCCGAGC42120.4272026240708191No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATCAT29290.2970741894357619No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACC26310.266849502357627No Hit
CCCGAAGCGTTTACTTTGAAAAAATTAGAGTGTTCAAAGCAGGCCC24070.24413027448681424No Hit
CCCGAAGCGTTTACTTTGAAAAAATTAGAGTGTTCAAAGCAGGCCCGA23030.23358206154679403No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCTA22470.2279022545790908No Hit
TGATCTAAAACACTCTTTACGCCGGCTTCTATTGACTTGGGTTAATCG21960.22272957323350395No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATC17550.17800109336284128No Hit
CTCTTTTTCCGGCTGGAACCATGGAGGG17360.17607401599879913No Hit
CCTTTCCGGCGGTGACGACCTACGCACACGAGAACATGCCTCTCGCAAA15330.15548471574087502No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTC14810.1502106092708649No Hit
TGATCTAAAACACTCTTTACGCCGGCTTCTATTGACTTGGG14640.1484863821556693No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA14340.14544362842297118No Hit
ATAGGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACATGC13510.13702534309583964No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACACA13140.1332726134921786No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTT12760.1294184587640943No Hit
TCAGTTCTTTGACCATTCGTTGGAGCTCCGGTTTTCAGCCTCTTTCCG12100.12272440055215839No Hit
ATTAATCCCCTGGCCCAACCCGTCATCTACTCTACCATCTTTGCAGGCAC11940.12110159856138604No Hit
CTCTTCCCTTTTGCGGCCATCACCGAAGCGGGAGCGGCCAAAATGAAGT11790.11958022169503697No Hit
CTTTTCGATCCGCCATCTGCGGTGGAGCCGCCACCAAAATGCAGATTTT11270.11430611522502684No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTTTTT11010.1116690619900218No Hit
CTCTTTCCCTAAGCAGCCTGAGGTGATCTGTGAAAATGGTTCGCTATTC10570.10720635651539785No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTA10480.10629353039558842No Hit
CCTTTCGGCCGGAACCGCCATCTTCCAGTAATTCGCCAAAATGACGAAC10400.10548212940020224No Hit
CCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCTAG10130.10274365104077392No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9990.10132369929884812No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGCC9970.10112084905000158No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTATA100.0013070123255.0940744
TAACCGT201.2733472E-7255.0940744
TCGTACC151.2910947E-5255.0940644
ATCGATG151.2910947E-5255.0940644
CCAACGA353.6379788E-11218.6520544
CATGACG204.067742E-5191.3205644
ATACCCG204.067742E-5191.3205644
CCCGAGC5900.0175.1069544
TATGGGG950.0161.1120544
CTATCAT5400.0155.8908144
GTGCAAC259.899974E-5153.0564444
TCGATTT259.899974E-5153.0564444
TGCACCT259.899974E-5153.0564444
CATGACC1050.0145.7680444
ACGAACA558.62201E-10139.1422144
TCTGGAC302.046444E-4127.5470344
CGAACGC205.4365682E-6120.08826443
TACGACG205.4365682E-6120.08826443
CCGGTTA152.6038676E-4120.0882643
GAGCGCC251.1413613E-7120.0882543
Produced by FastQC (version 0.11.5)