##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR841028.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	985949
Sequences flagged as poor quality	0
Sequence length	24-50
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.660332329562685	39.0	37.0	39.0	32.0	39.0
2	37.49688777005707	39.0	37.0	39.0	35.0	39.0
3	37.741368975474394	39.0	37.0	39.0	35.0	39.0
4	38.392655198189765	39.0	38.0	40.0	35.0	41.0
5	38.94724372153124	40.0	38.0	41.0	35.0	41.0
6	38.786354060909844	40.0	38.0	41.0	35.0	41.0
7	38.88974581849568	40.0	38.0	41.0	35.0	41.0
8	38.770117926992164	40.0	38.0	41.0	35.0	41.0
9	38.79632110788692	40.0	38.0	41.0	35.0	41.0
10	38.66855486439968	40.0	38.0	41.0	35.0	41.0
11	38.64964212144847	40.0	38.0	41.0	35.0	41.0
12	38.65216659279537	40.0	38.0	41.0	35.0	41.0
13	38.55977946120945	40.0	38.0	41.0	35.0	41.0
14	38.52070746052788	40.0	38.0	41.0	35.0	41.0
15	38.33935832380782	40.0	37.0	41.0	34.0	41.0
16	38.34803422895099	40.0	37.0	41.0	34.0	41.0
17	38.355386536220436	40.0	37.0	41.0	34.0	41.0
18	38.26420636361516	40.0	37.0	41.0	34.0	41.0
19	38.21404251132665	40.0	37.0	41.0	34.0	41.0
20	38.14242420246889	40.0	37.0	41.0	34.0	41.0
21	38.00434606658154	39.0	36.0	41.0	34.0	41.0
22	37.879360900005985	39.0	36.0	41.0	33.0	41.0
23	37.82729938363952	39.0	36.0	41.0	33.0	41.0
24	37.6173757466157	39.0	35.0	41.0	33.0	41.0
25	37.481309088089965	39.0	35.0	40.0	33.0	41.0
26	37.32009439610421	38.0	35.0	40.0	33.0	41.0
27	37.20112274551791	38.0	35.0	40.0	33.0	41.0
28	37.01391154188252	38.0	35.0	40.0	32.0	41.0
29	36.887179853755555	38.0	35.0	40.0	32.0	41.0
30	36.70556108670689	38.0	35.0	40.0	32.0	41.0
31	36.48735815356314	37.0	35.0	40.0	31.0	41.0
32	36.39855838176466	37.0	34.0	40.0	31.0	41.0
33	36.243298023450805	37.0	34.0	40.0	31.0	41.0
34	36.1683732522144	37.0	34.0	40.0	31.0	41.0
35	36.068823704618914	36.0	34.0	39.0	31.0	41.0
36	35.9816890865312	36.0	34.0	39.0	31.0	41.0
37	35.844380248752714	36.0	34.0	39.0	31.0	41.0
38	35.59114201802653	36.0	34.0	39.0	31.0	41.0
39	35.47357592479445	36.0	34.0	39.0	30.0	41.0
40	35.28052264083195	35.0	34.0	39.0	30.0	40.0
41	35.15127752886727	35.0	34.0	39.0	30.0	40.0
42	34.981071711094415	35.0	33.0	38.0	29.0	40.0
43	34.60803725500182	35.0	33.0	37.0	29.0	40.0
44	34.42924903578932	35.0	33.0	37.0	29.0	40.0
45	34.14066408988302	35.0	32.0	37.0	28.0	40.0
46	33.86518829434383	35.0	32.0	37.0	28.0	39.0
47	33.84512877881533	35.0	32.0	36.0	28.0	39.0
48	34.58405030999812	35.0	34.0	37.0	30.0	39.0
49	35.02252826553864	35.0	34.0	37.0	31.0	39.0
50	35.16719708801945	35.0	34.0	38.0	31.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	7.0
21	16.0
22	26.0
23	72.0
24	131.0
25	275.0
26	498.0
27	1012.0
28	2040.0
29	4360.0
30	8533.0
31	16276.0
32	29031.0
33	50897.0
34	77931.0
35	92380.0
36	141184.0
37	178271.0
38	161215.0
39	141043.0
40	80751.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	38.5444886094514	22.056211832457866	39.39929955809073
2	23.998604390287937	18.339183872593814	35.209934793787504	22.45227694333074
3	20.02649224249936	27.41967383708488	27.20465257330754	25.349181347108217
4	19.82871324987398	26.78566538431501	32.36070019848897	21.02492116732204
5	17.669169500653684	29.582463190286717	31.750729500207413	20.997637808852183
6	19.562168022889622	25.55111876983495	28.79155007003405	26.09516313724138
7	25.790887763971565	23.141866364284564	23.553246668945352	27.513999202798523
8	23.446953138549762	24.287463144645415	31.104752882755598	21.16083083404923
9	24.85625524241112	30.009970089730807	24.68758526049522	20.446189407362855
10	28.327225850424316	20.00417871512624	27.240557067353382	24.428038367096068
11	24.128529974674144	22.370325442796737	25.85306136524303	27.648083217286086
12	21.65324981312421	24.43838372978724	26.404509766732353	27.503856690356194
13	22.514247694353358	23.658424522972282	26.04110354592378	27.786224236750584
14	21.123404963137038	22.953519908230547	24.590115715924455	31.33295941270796
15	22.207436692972962	32.92817376963717	24.57449624676327	20.289893290626594
16	21.38041622842561	32.819344611131	24.503701509915825	21.296537650527565
17	21.616533918082983	33.782071892156694	22.147393019314386	22.454001170445935
18	22.838199541761288	24.261295462544208	23.505881135839683	29.394623859854818
19	22.498729650316598	24.559181052975358	23.100687763768715	29.841401532939333
20	22.752292461374775	24.083294369181367	24.74904888589572	28.415364283548133
21	22.370325442796737	33.374748592472834	24.021830743780864	20.23309522094956
22	21.85143450624728	27.378089536071336	22.063108740918647	28.707367216762737
23	25.294513205044073	24.962244497433435	29.97132711732554	19.77191518019695
24	23.99475023555985	26.047696179011286	23.891195183523692	26.06635840190517
25	24.52321496333835	24.96184903643315	24.805674904826606	25.709261095401892
26	23.03318343467496	34.47912184511932	23.171409235660615	19.316285484545105
27	24.846325240691673	27.047171020268117	22.66666528872666	25.43983845031355
28	25.00213510974464	24.28140538130977	24.04373121363804	26.67272829530755
29	24.045815970835207	27.070044276998246	27.90502100319148	20.979118748975072
30	26.265688236036315	26.831562127906373	28.626411901482594	18.276337734574717
31	24.426571407180823	31.7856875474604	24.20100749740639	19.586733547952385
32	22.69475863407481	24.921880896671446	24.793353676288238	27.590006792965504
33	22.934795721081272	24.818641531333853	34.12527889807953	18.121283849505346
34	23.05199035946156	32.43169551491579	25.531220605610656	18.985093520012
35	20.77507749671811	25.72935167514258	24.04152279672142	29.454048031417884
36	21.288394388222017	26.668273374263652	25.588077193044807	26.45525504446952
37	20.972195660415974	36.578906458824726	21.714024969216265	20.734872911543036
38	28.885100541246107	27.035186623006897	22.550495673406925	21.52921716234007
39	22.480273054256045	32.98234238564266	21.615812843117364	22.921571716983934
40	20.14255296278254	26.73525584288756	24.97295541817899	28.14923577615091
41	21.80560031324608	33.08026468831899	23.23184021677738	21.882294781657546
42	21.602016337757608	33.72983925151523	22.77190354389823	21.896240866828933
43	22.139106974298357	25.09300130397244	24.02589942712855	28.741992294600653
44	22.862055812207483	25.997905074442514	22.998165426462663	28.141873686887344
45	20.978834787865537	25.493565543986467	31.162060826671638	22.365538841476358
46	20.374827555842554	26.42387507704952	30.597904253133347	22.603393113974583
47	21.288772186054704	33.12910337350604	25.35783402301946	20.224290417419795
48	27.026898264746833	26.911258031474066	23.55509260963625	22.506751094142842
49	19.356789496161124	27.03173658107775	22.87646081478318	30.73501310797794
50	21.74575063879286	27.99056351006058	26.371852597321972	23.891833253824586
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	16460.0
1	14744.5
2	13716.0
3	13774.5
4	12009.0
5	7926.5
6	3847.0
7	2932.0
8	3266.5
9	3383.5
10	3267.0
11	3070.0
12	2877.0
13	2593.5
14	2459.5
15	2447.5
16	2435.0
17	2620.0
18	2661.5
19	2657.5
20	2959.5
21	3365.0
22	3743.5
23	4255.5
24	4821.5
25	5363.5
26	6045.5
27	7392.5
28	9243.5
29	10934.5
30	12302.5
31	15900.5
32	22812.0
33	26502.5
34	28753.0
35	33477.5
36	35752.5
37	37424.5
38	40587.0
39	43261.0
40	45537.5
41	48947.0
42	53592.0
43	57882.5
44	59336.0
45	57716.0
46	63204.5
47	70494.0
48	80170.0
49	93087.0
50	110421.5
51	123538.0
52	109490.0
53	91265.5
54	85374.0
55	81610.5
56	76885.5
57	72084.5
58	65553.0
59	56490.5
60	47196.0
61	38724.5
62	32124.5
63	25413.0
64	18274.0
65	13570.0
66	9438.0
67	6250.0
68	4663.0
69	3357.5
70	2531.0
71	1995.5
72	1515.5
73	1140.0
74	791.0
75	599.0
76	522.5
77	413.0
78	355.5
79	253.0
80	166.0
81	107.0
82	59.0
83	43.0
84	33.0
85	21.0
86	9.5
87	4.0
88	1.5
89	2.5
90	5.0
91	5.5
92	4.5
93	3.0
94	1.0
95	0.5
96	1.0
97	1.0
98	2.0
99	3.0
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
24	5633.0
25	6547.0
26	6141.0
27	7490.0
28	8854.0
29	8141.0
30	8153.0
31	7560.0
32	7291.0
33	6912.0
34	6737.0
35	6098.0
36	6251.0
37	5451.0
38	6521.0
39	7687.0
40	8709.0
41	9964.0
42	11466.0
43	18538.0
44	48350.0
45	96075.0
46	102131.0
47	117472.0
48	140980.0
49	167774.0
50	153023.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	13.03807991203727
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.79575344255228	8.969645309928099
2	11.116753896099333	2.8988225131958956
3	4.250570463979616	1.6625783214333474
4	2.315476289254537	1.207574595749127
5	1.5322015639569115	0.9988483216109351
6	1.049348511236054	0.8208893847043809
7	0.7497778743064203	0.6842964689039182
8	0.6877788735356773	0.7173852731975311
9	0.5107956979216847	0.5993815615404997
>10	5.375781876179467	16.950986807983615
>50	2.1606085469381475	20.156156770373354
>100	1.3747133308701764	29.276648069066702
>500	0.05791653588218414	5.230047952384805
>1k	0.020109908292425052	4.429830059613969
>5k	0.0	0.0
>10k+	0.0024131889950910063	5.396908590313822
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGC	25106	2.546379173770651	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTA	15707	1.5930844293163235	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCT	10646	1.0797718746101472	No Hit
CCCGAAGCGTTTACTTTGAAAAAATTAGAGTGTTCAAAGCAGGCCCGAGC	4212	0.4272026240708191	No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATCAT	2929	0.2970741894357619	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACC	2631	0.266849502357627	No Hit
CCCGAAGCGTTTACTTTGAAAAAATTAGAGTGTTCAAAGCAGGCCC	2407	0.24413027448681424	No Hit
CCCGAAGCGTTTACTTTGAAAAAATTAGAGTGTTCAAAGCAGGCCCGA	2303	0.23358206154679403	No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCTA	2247	0.2279022545790908	No Hit
TGATCTAAAACACTCTTTACGCCGGCTTCTATTGACTTGGGTTAATCG	2196	0.22272957323350395	No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATC	1755	0.17800109336284128	No Hit
CTCTTTTTCCGGCTGGAACCATGGAGGG	1736	0.17607401599879913	No Hit
CCTTTCCGGCGGTGACGACCTACGCACACGAGAACATGCCTCTCGCAAA	1533	0.15548471574087502	No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTC	1481	0.1502106092708649	No Hit
TGATCTAAAACACTCTTTACGCCGGCTTCTATTGACTTGGG	1464	0.1484863821556693	No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1434	0.14544362842297118	No Hit
ATAGGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACATGC	1351	0.13702534309583964	No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACACA	1314	0.1332726134921786	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTT	1276	0.1294184587640943	No Hit
TCAGTTCTTTGACCATTCGTTGGAGCTCCGGTTTTCAGCCTCTTTCCG	1210	0.12272440055215839	No Hit
ATTAATCCCCTGGCCCAACCCGTCATCTACTCTACCATCTTTGCAGGCAC	1194	0.12110159856138604	No Hit
CTCTTCCCTTTTGCGGCCATCACCGAAGCGGGAGCGGCCAAAATGAAGT	1179	0.11958022169503697	No Hit
CTTTTCGATCCGCCATCTGCGGTGGAGCCGCCACCAAAATGCAGATTTT	1127	0.11430611522502684	No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1101	0.1116690619900218	No Hit
CTCTTTCCCTAAGCAGCCTGAGGTGATCTGTGAAAATGGTTCGCTATTC	1057	0.10720635651539785	No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTA	1048	0.10629353039558842	No Hit
CCTTTCGGCCGGAACCGCCATCTTCCAGTAATTCGCCAAAATGACGAAC	1040	0.10548212940020224	No Hit
CCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCTAG	1013	0.10274365104077392	No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	999	0.10132369929884812	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGCC	997	0.10112084905000158	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.07535886744649065	0.0	0.0	0.0	0.0
2	0.1417923239437334	0.0	0.0	0.0	0.0
3	0.1836809003305445	0.0	0.0	0.0	0.0
4	0.226482302837165	0.0	0.0	0.0	0.0
5	0.2520414341918294	0.0	0.0	0.0	0.0
6	0.30082691903942294	0.0	0.0	0.0	0.0
7	0.3304430553710182	0.0	0.0	0.0	0.0
8	0.37060740464263364	0.0	0.0	0.0	0.0
9	0.3991078646055729	0.0	0.0	0.0	0.0
10	0.4285211506883216	0.0	0.0	0.0	0.0
11	0.45458740766510236	0.0	0.0	0.0	0.0
12	0.48724629772939576	0.0	0.0	0.0	0.0
13	0.5208180139134986	0.0	0.0	0.0	0.0
14	0.5514484014893265	0.0	0.0	0.0	0.0
15	0.5842087166780432	0.0	0.0	0.0	0.0
16	0.6137234278852152	0.0	0.0	0.0	0.0
17	0.6497293470554765	0.0	0.0	0.0	0.0
18	0.6819825366220768	0.0	0.0	0.0	0.0
19	0.7077445182255877	0.0	0.0	0.0	0.0
20	0.7374620796816063	0.0	0.0	0.0	0.0
21	0.7623112351653077	0.0	0.0	0.0	0.0
22	0.7901017192572841	0.0	0.0	0.0	0.0
23	0.8176893531004139	0.0	0.0	0.0	0.0
24	0.8419299578375757	0.0	0.0	0.0	0.0
25	0.8672862389433936	0.0	0.0	0.0	0.0
26	0.899945129007687	0.0	0.0	0.0	0.0
27	0.9287498643438961	0.0	0.0	0.0	0.0
28	0.958264575551068	0.0	0.0	0.0	0.0
29	0.9891992385001658	0.0	0.0	0.0	0.0
30	1.017598273338682	0.0	0.0	0.0	0.0
31	1.043461680066616	0.0	0.0	0.0	0.0
32	1.0708464636608992	0.0	0.0	0.0	0.0
33	1.0994483487482618	0.0	0.0	0.0	0.0
34	1.124906054978503	0.0	0.0	0.0	0.0
35	1.1448868044898874	0.0	0.0	0.0	0.0
36	1.1692288343514725	0.0	0.0	0.0	0.0
37	1.1891081587384338	0.0	0.0	0.0	0.0
38	1.2032062510332684	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTCTATA	10	0.0013070123	255.09407	44
TAACCGT	20	1.2733472E-7	255.09407	44
TCGTACC	15	1.2910947E-5	255.09406	44
ATCGATG	15	1.2910947E-5	255.09406	44
CCAACGA	35	3.6379788E-11	218.65205	44
CATGACG	20	4.067742E-5	191.32056	44
ATACCCG	20	4.067742E-5	191.32056	44
CCCGAGC	590	0.0	175.10695	44
TATGGGG	95	0.0	161.11205	44
CTATCAT	540	0.0	155.89081	44
GTGCAAC	25	9.899974E-5	153.05644	44
TCGATTT	25	9.899974E-5	153.05644	44
TGCACCT	25	9.899974E-5	153.05644	44
CATGACC	105	0.0	145.76804	44
ACGAACA	55	8.62201E-10	139.14221	44
TCTGGAC	30	2.046444E-4	127.54703	44
CGAACGC	20	5.4365682E-6	120.088264	43
TACGACG	20	5.4365682E-6	120.088264	43
CCGGTTA	15	2.6038676E-4	120.08826	43
GAGCGCC	25	1.1413613E-7	120.08825	43
>>END_MODULE