FastQCFastQC Report
Tue 24 May 2016
ERR841027.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR841027.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences903726
Sequences flagged as poor quality0
Sequence length24-50
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGC284073.14331998858061No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTA185482.052391986066573No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATCAT127791.4140347848794876No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCT110041.2176256962840506No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATC76400.8453889785178251No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCTA56860.6291730015513552No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGCC48990.5420890845234064No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTA47500.5256017863821557No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAG38160.42225187722827495No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTC35490.39270752418321486No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTT31460.3481143620964761No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACC31420.3476717500658385No Hit
CCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCTAG21730.24044898564387882No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACT20050.22185928035709937No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA17610.1948599464882055No Hit
TCAGTTCTTTGACCATTCGTTGGAGCTCCGGTTTTCAGCCTCTTTCCG16640.1841266047452436No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACACA16380.18124962654609916No Hit
CCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCT14640.16199600321336333No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAGAA14540.16088947313676932No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCT14260.1577911889223061No Hit
ATTAATCCCCTGGCCCAACCCGTCATCTACTCTACCATCTTTGCAGGCAC14170.1567953118533715No Hit
CTCTTTCCTTGCCTAACGCAGCCATGGCTCGTGGTCCCAAGAAGCATCT12310.13621385242872286No Hit
CCTTTCCGGCGGTGACGACCTACGCACACGAGAACATGCCTCTCGCAAA12240.13543928137510705No Hit
TCCCTGGTGTGATTCCGTCCTGCGCGGTTGTTCTCTGGAGCAGCGTTCT12190.13488601633681005No Hit
CTGATGTTCGCCGACCGTTGACTATTCTCTACAAACCACAAAGACATTGG11960.13234099716064382No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACA10260.11352998585854562No Hit
AATGCCCCAACTAAATACTACCGTATGGCCCACCATAATTACCCCCATAC10110.1118701907436546No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10000.11065300765940118No Hit
TCAGTTCTTTGACCATTCGTTGGAGCTCCGGTTTTCAGCCTCTTTC9490.10500970426877174No Hit
ATAGGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACATGC9420.10423513321515593No Hit
CGGCACAGCTGGTTTGAGCAACTGAACTGGAAACAAGATGCAGGACCC9340.10334990915388072No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCAGGG100.0015671775240.0849344
TATCTCG201.7228376E-7240.0849344
ACGGTGT100.0015671775240.0849344
CATGTGT151.6444777E-5240.0849244
ACGTACC151.6444777E-5240.0849244
CGGTCCA251.8153514E-9240.0849244
GCACTTA251.8153514E-9240.0849244
CCTCGAC255.23949E-7192.0679344
ACGCTTA255.23949E-7192.0679344
AGTCATA451.8189894E-12186.7327144
CTCAAGG451.8189894E-12186.7327144
CTATCAT17900.0173.022144
ACTCTTT600.0160.0566144
TAGCATG150.0052727526160.0566144
GCTTAAT301.2992441E-6160.0566144
AACGCCC150.0052727526160.0566144
ACTTCTA11350.0148.0699944
ATCCCGG352.7984715E-6137.1913944
CTACCTT352.7984715E-6137.1913944
AATGTAT352.7984715E-6137.1913944
Produced by FastQC (version 0.11.5)