##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR841027.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	903726
Sequences flagged as poor quality	0
Sequence length	24-50
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	37.00018147093256	39.0	37.0	39.0	33.0	39.0
2	37.628122904508665	39.0	37.0	39.0	35.0	39.0
3	37.929039332718105	39.0	38.0	39.0	35.0	39.0
4	38.50148828295302	39.0	38.0	40.0	35.0	41.0
5	39.12877797031401	40.0	38.0	41.0	35.0	41.0
6	39.11086546143411	40.0	38.0	41.0	35.0	41.0
7	39.07879821981441	40.0	38.0	41.0	35.0	41.0
8	39.067140925457494	40.0	38.0	41.0	35.0	41.0
9	39.003069514432475	40.0	38.0	41.0	35.0	41.0
10	38.919149166893504	40.0	38.0	41.0	35.0	41.0
11	38.8126755233334	40.0	38.0	41.0	35.0	41.0
12	38.774021108167744	40.0	38.0	41.0	35.0	41.0
13	38.6610676244791	40.0	38.0	41.0	35.0	41.0
14	38.60745292267789	40.0	38.0	41.0	35.0	41.0
15	38.56138364946898	40.0	38.0	41.0	35.0	41.0
16	38.546478689337256	40.0	38.0	41.0	35.0	41.0
17	38.55448222138126	40.0	38.0	41.0	35.0	41.0
18	38.46479131949285	40.0	37.0	41.0	35.0	41.0
19	38.42142861885129	40.0	37.0	41.0	34.0	41.0
20	38.20067365551063	40.0	37.0	41.0	34.0	41.0
21	38.110547887302126	40.0	37.0	41.0	34.0	41.0
22	38.050433427831	39.0	36.0	41.0	34.0	41.0
23	37.86525119339269	39.0	36.0	41.0	33.0	41.0
24	37.64658203924641	39.0	35.0	41.0	33.0	41.0
25	37.50258343045757	39.0	35.0	41.0	33.0	41.0
26	37.37327058549859	38.0	35.0	40.0	33.0	41.0
27	37.219744230797346	38.0	35.0	40.0	33.0	41.0
28	36.929690835842976	38.0	35.0	40.0	32.0	41.0
29	36.9145902965667	38.0	35.0	40.0	32.0	41.0
30	36.74334368453934	38.0	35.0	40.0	32.0	41.0
31	36.66494024180738	38.0	35.0	40.0	32.0	41.0
32	36.59653453242268	37.0	35.0	40.0	32.0	41.0
33	36.44178999229239	37.0	35.0	40.0	31.0	41.0
34	36.33959880402539	37.0	34.0	40.0	31.0	41.0
35	36.116190268253696	36.0	34.0	40.0	31.0	41.0
36	36.03703978028201	36.0	34.0	40.0	31.0	41.0
37	36.0156957164775	36.0	34.0	40.0	31.0	41.0
38	35.63680879232485	36.0	34.0	39.0	31.0	41.0
39	35.52851235391202	36.0	34.0	39.0	30.0	41.0
40	35.32486434303709	35.0	34.0	39.0	30.0	40.0
41	35.211455543337145	35.0	34.0	39.0	30.0	40.0
42	35.12536815803074	35.0	34.0	39.0	30.0	40.0
43	34.80612547261113	35.0	33.0	38.0	29.0	40.0
44	34.52132835313738	35.0	33.0	37.0	29.0	40.0
45	34.299161163168634	35.0	33.0	37.0	29.0	40.0
46	33.989482993261056	35.0	32.0	37.0	28.0	40.0
47	34.05524597670588	35.0	33.0	37.0	28.0	39.0
48	34.833087744025775	35.0	34.0	37.0	30.0	39.0
49	35.393360150789185	35.0	34.0	37.0	32.0	40.0
50	35.634342749228644	36.0	34.0	38.0	32.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	4.0
21	6.0
22	20.0
23	53.0
24	116.0
25	219.0
26	429.0
27	935.0
28	1981.0
29	4002.0
30	7549.0
31	14244.0
32	25511.0
33	43669.0
34	70539.0
35	80485.0
36	122758.0
37	162366.0
38	139226.0
39	129075.0
40	100539.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	40.41036774420566	20.102442554491073	39.48718970130327
2	23.453790197471356	17.88384975092008	37.77118285852128	20.891177193087284
3	22.87441104936673	26.834350234473725	25.664305331483217	24.626933384676327
4	19.789294542814968	28.982678378181	27.100802676917564	24.127224402086473
5	17.278799105038477	29.633760675248915	27.60161818958401	25.485822030128602
6	20.538304751661453	23.612466610454938	24.87114457258063	30.97808406530298
7	21.023739496263246	22.723922958949945	20.96055662888973	35.29178091589708
8	22.62488851709478	24.444909187076615	30.0096489422679	22.920553353560702
9	22.091983632207107	31.11086767449426	26.588257945439214	20.208890747859417
10	33.42727773683617	19.09804520396669	24.950814738095396	22.523862321101753
11	24.142937129174108	21.216607688613585	22.85283371287315	31.787621469339157
12	20.704616222173534	25.90320517501986	23.610806815340048	29.781371787466554
13	23.986584429351375	22.036878434392726	22.27422913582214	31.70230800043376
14	17.413574468367624	24.183546782985108	22.891783571569256	35.511095177078005
15	21.128306588501385	36.99240699061441	21.74375861710297	20.135527803781233
16	17.62093820472134	36.6956356240719	20.954028101437824	24.729398069768934
17	21.362559005716335	41.04673319125487	17.476204070702845	20.114503732325947
18	18.83004361941562	28.999608288352885	19.30529828731275	32.86504980491875
19	24.472572438991463	24.036488935805764	18.823072479933078	32.6678661452697
20	19.88290698729482	22.96005647729511	25.32714561714502	31.829890918265047
21	19.355313446774797	38.09240853975652	20.793138628301055	21.75913938516763
22	26.725357021929213	23.98713769438967	19.75565602848651	29.531849255194604
23	25.767765893644757	24.305486397425767	29.413450536999047	20.513297171930432
24	22.541234843304274	25.581315575738667	23.430553065862885	28.446896515094178
25	21.059573483749855	24.82217368481926	21.569030863263908	32.549221968166975
26	21.735235600747515	36.17100276124769	23.448349905665758	18.64541173233903
27	23.773084964365285	26.336400269467507	20.728979341199175	29.161535424968033
28	23.345591774650057	22.58698038007586	21.43773734920928	32.6296904960648
29	21.476751311436686	29.642177766569404	28.501966727108197	20.379104194885713
30	24.548436294145752	26.885496674139137	28.39040277828819	20.175664253426923
31	25.193702557035607	33.34955710265831	21.11976378808443	20.336976552221657
32	20.90714121046859	29.419216322766783	22.109063727624772	27.56457873913985
33	20.480214407369317	23.854576889828067	34.00940843526195	21.655800267540666
34	23.197169636764553	34.62846179139271	22.883562184668946	19.2908063871738
35	18.05595273551849	24.66066328328668	22.980206396462997	34.303177584731834
36	19.37668518755234	24.987187497386728	25.681750576114627	29.954376738946305
37	20.589574555253993	36.918333976108	21.305277119985007	21.186814348652998
38	30.781600440099872	23.402673243983386	20.647333708143663	25.168392607773082
39	19.657610582281258	35.414710199209495	23.064086870200185	21.863592348309062
40	18.359441208765563	25.75566271097759	26.657545081836336	29.22735099842051
41	18.857871233427577	33.0986737097651	22.365354111797604	25.678100945009717
42	18.597938816776466	36.12903468588029	20.711303894070838	24.561722603272404
43	19.957829034633352	23.57796986734459	21.307274491525746	35.15692660649631
44	19.37107884427861	24.46657961304971	21.3473608563368	34.814980686334884
45	18.98233117803672	22.2697693717368	36.3589087514263	22.38899069880018
46	18.159687880364555	26.137050769439902	32.47506683478878	23.228194515406766
47	18.894895826409353	33.89445269167424	26.893662434729947	20.316989047186464
48	27.52789192850148	24.19573315272604	20.836345997199366	27.440028921573113
49	15.691246337326728	29.450084802055482	21.343480357281344	33.51518850333645
50	19.1968560485158	27.881290562825832	29.775109054154697	23.146744334503673
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	15847.0
1	12498.0
2	8580.0
3	7434.5
4	6143.0
5	4199.0
6	2136.0
7	1429.5
8	1729.0
9	1997.5
10	2103.5
11	2147.5
12	2233.0
13	2381.0
14	2523.5
15	2518.0
16	2472.5
17	2756.5
18	3235.0
19	3464.5
20	3639.5
21	3998.0
22	4142.5
23	4218.0
24	4638.0
25	5301.5
26	5899.5
27	6107.0
28	6393.5
29	7610.5
30	9949.0
31	17055.5
32	28083.0
33	30277.5
34	26610.0
35	27437.0
36	26903.5
37	24671.0
38	25015.0
39	27900.5
40	31320.0
41	34053.0
42	37122.0
43	38965.5
44	38910.0
45	41894.5
46	49122.5
47	54058.0
48	66066.5
49	88588.5
50	115543.5
51	133489.5
52	118919.5
53	88811.0
54	73548.5
55	70714.5
56	69851.0
57	68499.5
58	66736.5
59	61120.0
60	51330.5
61	43126.5
62	36429.5
63	29424.0
64	23490.5
65	19654.0
66	14658.0
67	9696.5
68	7024.5
69	5143.5
70	3930.0
71	2932.0
72	2076.0
73	1414.5
74	991.5
75	671.0
76	421.5
77	277.0
78	228.0
79	255.5
80	219.0
81	121.0
82	23.5
83	6.0
84	4.5
85	3.5
86	3.0
87	1.5
88	1.0
89	1.0
90	1.0
91	1.0
92	1.0
93	1.0
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
24	4534.0
25	5031.0
26	5000.0
27	6232.0
28	6600.0
29	5577.0
30	5766.0
31	6112.0
32	5172.0
33	5189.0
34	5766.0
35	5674.0
36	4744.0
37	5244.0
38	6090.0
39	6809.0
40	8480.0
41	8494.0
42	10889.0
43	18214.0
44	45084.0
45	83014.0
46	98415.0
47	104552.0
48	140471.0
49	146189.0
50	150384.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	11.905996285233563
#Duplication Level	Percentage of deduplicated	Percentage of total
1	66.27235438774105	7.890384051541272
2	11.832016334822605	2.8174388505844155
3	4.697892106261814	1.6779925789674373
4	2.608560786797249	1.2423006014965594
5	1.5653717366291526	0.9318655040658153
6	1.1343714866042527	0.8103493623351065
7	0.9067501021922177	0.755703434383499
8	0.727638907845132	0.6930612907036439
9	0.5681180654604937	0.6087610419252045
>10	5.930257042243067	16.679785970905886
>50	2.1997083695913173	18.737755927988818
>100	1.4897940060378854	29.3480354900718
>500	0.04197916735861292	3.4483130907986013
>1k	0.01959027810068603	5.021473351631254
>5k	0.0018657407714939075	1.4800856845422155
>10k+	0.003731481542987815	7.8566937680584745
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGC	28407	3.14331998858061	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTA	18548	2.052391986066573	No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATCAT	12779	1.4140347848794876	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCT	11004	1.2176256962840506	No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATC	7640	0.8453889785178251	No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCTA	5686	0.6291730015513552	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGCC	4899	0.5420890845234064	No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTA	4750	0.5256017863821557	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAG	3816	0.42225187722827495	No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTC	3549	0.39270752418321486	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTT	3146	0.3481143620964761	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACC	3142	0.3476717500658385	No Hit
CCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCTAG	2173	0.24044898564387882	No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACT	2005	0.22185928035709937	No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1761	0.1948599464882055	No Hit
TCAGTTCTTTGACCATTCGTTGGAGCTCCGGTTTTCAGCCTCTTTCCG	1664	0.1841266047452436	No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACACA	1638	0.18124962654609916	No Hit
CCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCT	1464	0.16199600321336333	No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAGAA	1454	0.16088947313676932	No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCT	1426	0.1577911889223061	No Hit
ATTAATCCCCTGGCCCAACCCGTCATCTACTCTACCATCTTTGCAGGCAC	1417	0.1567953118533715	No Hit
CTCTTTCCTTGCCTAACGCAGCCATGGCTCGTGGTCCCAAGAAGCATCT	1231	0.13621385242872286	No Hit
CCTTTCCGGCGGTGACGACCTACGCACACGAGAACATGCCTCTCGCAAA	1224	0.13543928137510705	No Hit
TCCCTGGTGTGATTCCGTCCTGCGCGGTTGTTCTCTGGAGCAGCGTTCT	1219	0.13488601633681005	No Hit
CTGATGTTCGCCGACCGTTGACTATTCTCTACAAACCACAAAGACATTGG	1196	0.13234099716064382	No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACA	1026	0.11352998585854562	No Hit
AATGCCCCAACTAAATACTACCGTATGGCCCACCATAATTACCCCCATAC	1011	0.1118701907436546	No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1000	0.11065300765940118	No Hit
TCAGTTCTTTGACCATTCGTTGGAGCTCCGGTTTTCAGCCTCTTTC	949	0.10500970426877174	No Hit
ATAGGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACATGC	942	0.10423513321515593	No Hit
CGGCACAGCTGGTTTGAGCAACTGAACTGGAAACAAGATGCAGGACCC	934	0.10334990915388072	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.020913418447626824	0.0	0.0	0.0	0.0
2	0.05200691359991856	0.0	0.0	0.0	0.0
3	0.07524404520839281	0.0	0.0	0.0	0.0
4	0.11375129187386443	0.0	0.0	0.0	0.0
5	0.16929910171888382	0.0	0.0	0.0	0.0
6	0.20802765439967424	0.0	0.0	0.0	0.0
7	0.24642424805748644	0.0	0.0	0.0	0.0
8	0.29610744849655757	0.0	0.0	0.0	0.0
9	0.3445734658513753	0.0	0.0	0.0	0.0
10	0.3784332861951521	0.0	0.0	0.0	0.0
11	0.40056388772703233	0.0	0.0	0.0	0.0
12	0.4368580742393159	0.0	0.0	0.0	0.0
13	0.46739830435331065	0.0	0.0	0.0	0.0
14	0.49782788145964596	0.0	0.0	0.0	0.0
15	0.5334581499259732	0.0	0.0	0.0	0.0
16	0.570637560499532	0.0	0.0	0.0	0.0
17	0.6261853703445514	0.0	0.0	0.0	0.0
18	0.6754259587529848	0.0	0.0	0.0	0.0
19	0.743034946432879	0.0	0.0	0.0	0.0
20	0.7732432175238955	0.0	0.0	0.0	0.0
21	0.8107545871204325	0.0	0.0	0.0	0.0
22	0.833770412713588	0.0	0.0	0.0	0.0
23	0.8678515390726835	0.0	0.0	0.0	0.0
24	0.8942976079032804	0.0	0.0	0.0	0.0
25	0.9193051876343051	0.0	0.0	0.0	0.0
26	0.9633450846827467	0.0	0.0	0.0	0.0
27	0.9851437271916488	0.0	0.0	0.0	0.0
28	1.0171224464052158	0.0	0.0	0.0	0.0
29	1.0440111272664503	0.0	0.0	0.0	0.0
30	1.0707891551200253	0.0	0.0	0.0	0.0
31	1.0972352239506222	0.0	0.0	0.0	0.0
32	1.1210256205973934	0.0	0.0	0.0	0.0
33	1.1435988341599113	0.0	0.0	0.0	0.0
34	1.167278577799023	0.0	0.0	0.0	0.0
35	1.189851791361541	0.0	0.0	0.0	0.0
36	1.2093267207095957	0.0	0.0	0.0	0.0
37	1.2278057729887155	0.0	0.0	0.0	0.0
38	1.2395349918006122	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTCAGGG	10	0.0015671775	240.08493	44
TATCTCG	20	1.7228376E-7	240.08493	44
ACGGTGT	10	0.0015671775	240.08493	44
CATGTGT	15	1.6444777E-5	240.08492	44
ACGTACC	15	1.6444777E-5	240.08492	44
CGGTCCA	25	1.8153514E-9	240.08492	44
GCACTTA	25	1.8153514E-9	240.08492	44
CCTCGAC	25	5.23949E-7	192.06793	44
ACGCTTA	25	5.23949E-7	192.06793	44
AGTCATA	45	1.8189894E-12	186.73271	44
CTCAAGG	45	1.8189894E-12	186.73271	44
CTATCAT	1790	0.0	173.0221	44
ACTCTTT	60	0.0	160.05661	44
TAGCATG	15	0.0052727526	160.05661	44
GCTTAAT	30	1.2992441E-6	160.05661	44
AACGCCC	15	0.0052727526	160.05661	44
ACTTCTA	1135	0.0	148.06999	44
ATCCCGG	35	2.7984715E-6	137.19139	44
CTACCTT	35	2.7984715E-6	137.19139	44
AATGTAT	35	2.7984715E-6	137.19139	44
>>END_MODULE